Participação das citocinas Interleucina-12, Interferon-γ, Interleucina-4 e Interleucina-10 e investigação de polimorfismos nos genes do INF-γ (IFNG+874) e da IL-10 (IL10-1082) na malária causada por Plasmodium vivax

A resposta imune na malária é complexa, e os mecanismos de ativação e regulação de linfócitos T efetores e de memória ainda são pouco compreendidos. No presente estudo, determinamos a concentração das citocinas Interferon-γ (IFN-γ), Interleucina-10 (IL-10), Interleucina-4 (IL-4) e Interleucina-12 (IL-12) no soro de indivíduos infectados por Plasmodium vivax, investigamos os polimorfismos no gene do IFN-γ (IFNG+874) e da IL-10 (IL10-1082) e analisamos a associação destes polimorfismos com a concentração das citocinas e com a densidade parasitária. A concentração das citocinas foi determinada por ELISA, e a genotipagem dos polimorfismos IFNG+874 e IL10-1082 foi realizada pelas técnicas de ASO-PCR e PCR-RFLP, respectivamente. Os indivíduos infectados apresentaram níveis séricos de IFN-γ e IL-10 aumentados. A produção de IFN-γ foi maior nos indivíduos primoinfectados, porém não foi associada com a redução da parasitemia. A produção de IL-10 foi alta e associada com altas parasitemias. As citocinas IL-4 e IL-12 não foram detectadas. As freqüências dos genótipos homozigoto mutante AA, heterozigoto AT e selvagem TT do gene do IFN-γ foram 0,51, 0,39 e 0,10, respectivamente. As freqüências dos genótipos homozigoto mutante AA, heterozigoto AG e selvagem GG para IL10 foram 0,49, 0,43 e 0,08, respectivamente. Apenas o polimorfismo do IFN-γ foi associado com níveis reduzidos desta citocina. Na malária causada por P. vivax, houve produção de citocina que caracteriza o perfil Th1 (IFN-γ), com possível participação da IL-10 na imunorregulação.

Análise de associações de polimosfismos HPA, indicadores de autoimunidade e manifestações reumatológicas na Hepatite C

Pós-graduação em Fisiopatologia em Clínica Médica - FMB

Molecular characterization of accessions of crabgrass (Digitaria nuda) and response to ametryn

Digitaria species are sugar cane crop weeds in Brazil and are being controlled with herbicides, although there are some reports of control failure, notably to the triazine group. Molecular techniques are recommended to analyze the genetic variability in weeds. RAPD (Random Amplified Polymorphic DNA), PCR-RFLP (Polymerase Chain Reaction - Restriction Fragment Length Polymorphism) and, in combination with sequencing, allow the localization of resistance genes, as well as possible mutations related to the onset of resistant individuals in some species. Thus, the objective of this work was to characterize ten accessions of Digitaria spp. by RAPD and PCR-RFLP markers, to sequence a conserved region of the psbA gene and evaluate the accessions response to ametryn. As showed by molecular analysis there was high genetic similarity among the accessions, all of them presented similar genetics profiles and were susceptible to ametryn.

Association among XRCC1, XRCC3, and BLHX gene polymorphisms and chromosome instability in lymphocytes from patients with endometriosis and ovarian cancer

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

High occurrence of Cryptosporidium ubiquitum and Giardia duodenalis genotype E in sheep from Brazil

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Occurrence of Mycobacterium bovis and non-tuberculous mycobacteria (NTM) in raw and pasteurized milk in the northwestern region of Paraná, Brazil

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Polimorfismos dos genes ADA e CNTNAP2 em indivíduos com Transtornos do Espectro do Autismo

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Association of Interleukin 4 Gene Polymorphisms With Dental Implant Loss

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Diferentes métodos de diagnóstico molecular na diferenciação das espécies de Sarcocystis spp

The Sarcocystis genus includes obligatory two-host life cycle protozoan parasites. It is the most numerous of the six genera of the Sarcocystidae family. The infection caused by parasites of this genus is a zoonotic and cosmopolitan disease known as sarcosistosis or sarcosporidiosis. The sarcositosis though frequently asymptomatic in its definitive hosts can be fatal in its intermediate hosts. The usual diagnoses of sarcosistosis takes place through a histological demonstration of schizonts in blood vessels and organs, and the presence of cysts in muscle tissue by necropsy or biopsy, this second method still more common and based on morphological features of the sarcocyst. However, these methods can be inadequate to a precise identification of the infector species once that, besides the genus being of numerous species, these often present similar morphological features. Another factor that makes the diagnostic more difficult is the non specificity of some Sacocystis species to their hosts. Consequently, molecular diagnostic methods have been used in order to identify the infector species and the parasite specific biological cycles, demonstrating also new species and coevolutive aspects between parasite and host. Among the most employed molecular techniques the Polimerase Chain Reaction (PCR), the nested-PCR and the Restriction Fragment Length Polymorphism (RFLP) stands out

Prevalence of the germline TP53 p.R337H mutation in families with multiple cases of cancer

Germline mutations in TP53 gene are associated with Li-Fraumeni syndrome (LFS) and its variants Li-Fraumeni-like (LFL). They predispose carriers to a wide variety of early onset tumors. In Brazil, there is a high frequency of a germline mutation in this gene (NC_000017.9: c.1010G>A; p.R337H) in Southern and Southeastern regions, due to a founder effect. It is estimated to be present in 0,3% ofthe local population, but only few families have been detected. Due to this significant divergence, the purpose of this study was to verify the effectiveness of wider criteria for detection of these individuals. Herein, clinical criteria were established, DNA samples were collected, analyzed by Restriction Fragment Length Polymorphism (RFLP) and sequenced. Thus, assessing the prevalence of this mutation in families with multiple cases of cancer. Based on our proposed criteria, one out of 31 patients (3,22%) was found to carry p.R337H mutation. The patient developed ductal invasive breast cancer at age 47, invasive adenocarcinoma of the lung at age 48 and soft-tissue sarcoma at age 49. In addition, an extensive cancer family history was referred, atypical for LFS, including a case of Ewing’s sarcoma. These outcomes indicate that the proposed criteria may detect probable carriers who did not fit previous LFS criteria. Nevertheless, additional studies, which might include a larger number of families and more stringent parameters, will be useful to improve screening sensibility

Ocorrência de Angiostrongylus cantonensis (Nematoda, Angiostrongylidae) em Achatina fulica (Mollusca, Gastropoda) na Baixada Santista

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Achados clínicos, genéticos e microbiológicos de uma família com periodontite agressiva de Maceió

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

An atypical Toxoplasma gondii genotype in a rural Brazilian dog co-infected with Leishmania (Viannia) braziliensis

Toxoplasmosis and leishmaniasis are two worldwide zoonoses caused by the protozoan parasites Toxoplasma gondii and Leishmania spp., respectively. This report describes the clinical and laboratorial findings of a co-infection with both parasites in a 4-year-old female dog suspected of ehrlichiosis that presented anemia, thrombocytopenia, hypoalbuminemia, hyperglobulinemia, tachyzoite-like structures to the lung imprints, and polymerase chain reaction (PCR) results positive for T. gondii (kidney, lung, and liver) and Leishmania spp. Co-infection with Toxoplasma gondii and Leishmania braziliensis was confirmed by sequencing; restriction fragment length polymorphism-polymerase chain reaction (RFLP-PCR) confirmed an atypical T. gondii genotype circulating in dogs that has been reported to cause human congenital toxoplasmosis.

TLR2 and TLR4 polymorphisms influence mRNA and protein expression in colorectal cancer

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Influence of functional polymorphisms in TNF-α, IL-8, and IL-10 cytokine genes on mRNA expression levels and risk of gastric cancer

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)