Influência das variações de ângulos de projeção na detecção de reabsorções radiculares linguais e apicais simuladas: comparação entre subtração radiográfica digital e radiografia digital direta

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Produção in vitro de embriões bovinos do sexo feminino por sexagem de espermatozóides em gradiente de densidade, modificações na maturação e na fecundação in vitro

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Viabilidade espermática e geração de metabólicos reativos do oxigênio(ROS) no sêmen ovino criopreservado em diluidor aditivado de lauril de sódio (OE), trolox-C e catalase

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Caracterização imunofenotípica e potencial de diferenciação de células-tronco mesenquimais provenientes de medula óssea, tecido adiposo e cordão umbilical de equinos

Pós-graduação em Medicina Veterinária - FMVZ

Caracterização molecular de genes do sistema imune de búfalo

Pós-graduação em Genética e Melhoramento Animal - FCAV

Efeito da curcumina na angiogênese em modelo experimental de câncer de mama

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Utilização da técnica de imuno-histoquímica para confirmar casos de pitiose cutânea equina diagnosticados por meio de caracterização clínica e avaliação histopatológica

Twelve granulomatous lesions in the thoracic and pelvic limbs of horses from pantaneira breed were evaluated. It was performed suggestive diagnostic of cutaneous pythiosis according to the clinic characteristics of the lesions and histopathological exams using hematoxylin and eosin (HE) and Grocott's methenamine silver (GMS) stains. The confirmation of the diagnoses was performed by the labelled streptavidin biotin (LSAB) method (immunohistochemistry). Extensive focal pyogranulomatous dermatitis associated with pseudo hyphae characteristic of Pythium insidiosum (subcutaneous pythiosis) was the histopathological diagnosis for all 12 horses used in this study. It was confirmed in 100% of the cases by the positive result to Pythium insidiosum in the immunohistochemistry exam, characterized by the visualization of branched and septated structures. It was observed 100% of parity between the techniques. We can conclude that clinical characterization of the granulomatous lesions with pythiosis aspects in horses associated with histopathology suggestive of equine pythiosis constitute a reliable method of diagnostic that can be confirmed by immunohistochemistry technique.

Avaliação in vitro da resistência estrutural em dentes fragilizados utilizando reforços intra-radiculares

Pós-graduação em Odontologia Restauradora - ICT

Expressão da proteína nuclear de proliferação celular (PCNA), da B-Catenina e do Agnor, na etapa inicial do processo de carcinogênese quimicamente induzido pelo DMBA, em borda lateral de língua de hamsters sírio dourado(Mesocricetus auratus)

The oral cancer model in hamsters shows many simílarities with developmental oral cancer in humans. The proliferating capacity is one the most characteristics of neoplásica ce/Is and detection of these ce/Is allow us, throughout of its counting, to achieve an estimated tumour growing index, with a consequent repercussion about prognostic and in the treatment of those lesions. 40 golden Syrian hamsters, both genders, aged between two to six mouth and weight 150g in average were used. The left síde of tangue of each animal was painted for eight consecutive weeks, with a solution of DMBA. Tongues were removed, fixed in 10% buffered fonnal solution. The histological slides were stained by AgNOR technique and by PCNA and β-catenin immunohistochemical antibodies. Statistical analyzes were performed by ANOVA one-way test and Tukey test. We may conclude that an association between AgNOR and PCNA might indicate the higher proliferating activity of the analyzed celIs. The experimental carcinogenesis model in hamster tongue is an available methodology for immunohistochemistry study. And finally, PCNA and β-catenin immunohistochemical antibodies may be used to analyze possible premalignant areas in oral leukoplakia

Caracterização de Aeromonas spp isoladas de amostras de ostras e água por método microbiológico e molecular

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

AVALIAÇÃO ROBUSTA DA MODELAGEM NEUTROSFÉRICA SOBRE O TERRITÓRIO BRASILEIRO BASEADA EM MODELOS DE PREVISÃO NUMÉRICA DE TEMPO DA AMÉRICA DO SUL

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Comparison of the performance of natural latex membranes prepared with different procedures and PTFE membrane in guided bone regeneration (GBR) in rabbits

This work assessed the performance of membranes made of natural latex extracted from Hevea brasiliensis prepared with three different methods: polymerized immediately after collection without the use of ammonia (L1); polymerized after preservation in ammonia solution (L2); and polymerized after storage in ammonia, followed by Soxhlet technique for the extraction of substances (L3). Polytetrafluoroethylene (PTFE) membrane was used as control. Two 10-mm diameter bone defects were surgically made in the calvaria of thirty adult male New Zealand rabbits. Defects (total n = 60) were treated with guided bone regeneration (GBR) using L1, L2, L3 or PTFE membranes (n = 15 for each membrane). Ten animals were euthanized after 7, 20 and 60 days postoperatively so that five samples (n = 5) of each treatment were collected at each time, and bone regeneration was assessed microscopically. The microscopic analysis revealed defects filled with blood clot and new bone formation at the margins of the defect in all 7-day samples, while 20-day defects were mainly filled with fibrous connective tissue. After 60 days defects covered with L1 membranes showed a significantly larger bone formation area in comparison to the other groups (P < 0.05, ANOVA, Tukey). Additionally, bone tissue hypersensitization for L1 and PTFE membranes was also investigated in six additional rabbits. The animals were subjected to the same surgical procedure for the confection of one 10-mm diameter bone defect that was treated with L1 (n = 3) or PTFE (n = 3). Fifty-three days later, a second surgery was performed to make a second defect, which was treated with the same type of membrane used in the first surgery. Seven days later, the animals were euthanized and samples analyzed. No differences among L1 and PTFE samples collected from sensitized and non-sensitized animals were found (P > 0.05, Kruskal-Wallis). Therefore, the results demonstrated that latex membranes presented performance comparable to PTFE membranes, and that L1 membranes induced higher bone formation. L1 and PTFE membranes produced no hypersensitization in the bone tissue.

Structural, dielectric, ferroelectric and optical properties of PBCT, PBST and PCST complex thin films on LaNiO3 metallic conductive oxide layer coated Si substrates by the CSD technique

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Crescimento, composição mineral e sintomas visuais de deficiência nutricional em ipeca

The objective was to evaluate the effect of the omission of macronutrient and micronutrient boron in dry matter production, the characterization of the symptoms of nutritional deficiencies and mineral composition in plants of ipeca, an experiment was conducted in greenhouse conditions by the technique the missing element and visual diagnosis. A randomized block experimental design with four repetitions and the treatments were: complete and omissions of N, P, K, Ca, Mg, S and B. The analytical results demonstrated that the production of dry matter was affected in all of the treatments with omission of nutrients and that the ipeca plants presented characteristic symptoms of nutritional deficiencies due to the omissions of N, P, K, Ca, Mg, S and B and the concentrations of the macronutrients and of the micronutrient boron in the different parts of the ipeca plant varied when a certain nutrient was omitted in the solution.

A metacognição de usuários no processo de busca da informação em catálogo coletivo de biblioteca universitária

It was observed the metacognitive trajectory of three users from a public university library the state of Sao Paulo to identify the metacognitive strategies that were used during the process of searching for information in a online collective catalog. Its configuration was based on participants' oral reports obtained by the technique of individual verbal protocol to provide an actual configuration of the task ans a closer examination of its natural recovery of information systems. The data collection sessions were recorded, and the recorded data were transcribed and categorized into four units of analyses: 1- expertise, 2-regulation, 3-assessment of knowledge itself and 4- organization of cognitive process. The results showed that knowledge of meta-cognitive strategies affects the quality of the results obtained in the process of searching for information and interferes in the way it implements retrieval on-line catalog, a fact the emphasizes the need for studies to move forward on issues meta-cognition of users of information.