Limited effects of increased CO2 and temperature on metal and radionuclide bioaccumulation in a sessile invertebrate, the oyster Crassostrea gigas

This study investigated the combined effects of reduced pH and increased temperature on the capacities of the Pacific cupped oyster Crassostrea gigas to bioconcentrate radionuclide and metals. Oysters were exposed to dissolved radiotracers (110mAg, 241Am, 109Cd,57Co,54Mn, and 65Zn) at three pH (7.5, 7.8, 8.1) and two temperatures (21 and 24°C) under controlled laboratory conditions. Although calcifying organisms are recognized as particularly vulnerable to ocean acidification, the oyster did not accumulate differently the studied metals when exposed under the different pH conditions. However, temperature alone or in combination with pH somewhat altered the bioaccumulation of the studied elements. At pH 7.5, Cd was accumulated with an uptake rate constant twofold higher at 24°C than 21°C. Bioaccumulation of Mn was significantly affected by an interactive effect between seawater pH and temperature, with a decreased uptake rate at pH 7.5 when temperature increased (27 ± 1 vs. 17 ± 1 /day at 21 and 24°C, respectively). Retention of Co and Mn tended also to decrease at the same pH with decreasing temperature. Neither pH nor temperature affected strongly the elements distribution between shell and soft tissues. Significant effects of pH were found on the bioaccessibility of Mn, Zn, and 241Am during experimental in vitro simulation of human digestion.

Nouueau commentaire sur l'ordonnance de la marine, du mois d'Août 1681 : tome second / par M. ***, auocat en Parlement.

Aut. tomado del catálogo en línea de la Bibliothèque nationale de France, 06-05-2015

Nouueau commentaire sur l'ordonnance de la marine, du mois d'Août 1681 : tome second / par M. ***, auocat en Parlement.

Aut. tomado del catálogo en línea de la Bibliothèque nationale de France, 06-05-2015

Telomerase inhibitors based on quadruplex ligands selected by a fluorescence assay

The reactivation of telomerase activity in most cancer cells supports the concept that telomerase is a relevant target in oncology, and telomerase inhibitors have been proposed as new potential anticancer agents. The telomeric G-rich single-stranded DNA can adopt in vitro an intramolecular quadruplex structure, which has been shown to inhibit telomerase activity. We used a fluorescence assay to identify molecules that stabilize G-quadruplexes. Intramolecular folding of an oligonucleotide with four repeats of the human telomeric sequence into a G-quadruplex structure led to fluorescence excitation energy transfer between a donor (fluorescein) and an acceptor (tetramethylrhodamine) covalently attached to the 5′ and 3′ ends of the oligonucleotide, respectively. The melting of the G-quadruplex was monitored in the presence of putative G-quadruplex-binding molecules by measuring the fluorescence emission of the donor. A series of compounds (pentacyclic crescent-shaped dibenzophenanthroline derivatives) was shown to increase the melting temperature of the G-quadruplex by 2–20°C at 1 μM dye concentration. This increase in Tm value was well correlated with an increase in the efficiency of telomerase inhibition in vitro. The best telomerase inhibitor showed an IC50 value of 28 nM in a standard telomerase repeat amplification protocol assay. Fluorescence energy transfer can thus be used to reveal the formation of four-stranded DNA structures, and its stabilization by quadruplex-binding agents, in an effort to discover new potent telomerase inhibitors.

Ordonnances des roys de France de la troisième race, recueillies par ordre chronologique.

Index B: Table chronologique des ordonnances des rois de France de la troisième race jusqu'au règne de Louis XII inclusivement, suivie d'une table alphabétique pour en faciliter l'usage, par J. M. Pardessus ... . Paris, Imprimerie royale, 1847. 2 p. l., viii, 607 p. 40 cm.

Ëtudes de moeurs et de critique sur les Poëtes Latins de la Décadence /

Contiene: Vol. 1- Vol. 2.

Historia de una enfermedad freqüente, aunque poco conocida, propia del sistema linfático y consideraciones generales sobre las afecciones de los absorventes /

Mode of access: Internet.

Les grecs anciens et les grecs modernes /

Mode of access: Internet.

Maître italien au Grammaire française et italienne de Vénéroni ... /

Mode of access: Internet.

Démonstrations élémentaires de botanique, a l'usage de l'École Royale Vétérinaire ; Tome premier : introduction a la botanique contenant un abrégé des principes [et] de lñ'histoire de cette science [et] les élémens de la physique des végétaux : suivie d'une instruction sur la formation d'un herbier ...

Mode of access: Internet.

Démonstrations élémentaires de botanique : tome premier : introduction a la botanique contenant un abrégé des principes & de l'histoire de cette science & les élémens de la physique des végétaux : suivie d'une instruction sur la formation d'un herbier ...

Mode of access: Internet.

Démonstrations élémentaires de botanique... : tome second contenant la description des plantes usuelles... /

Port. a dos tintas.

Démonstrations élémentaires de botanique contenant les principes généraux de cette science, les fondements des méthodes ... : la description des plantes ... suivant la méthode de Tournefort & celle de Linné ... /

Grab. xil. en cabecera de texto.

Exhortations et instructions chretiennes :

Mode of access: Internet.

Exhortations et instructions chretiennes :

Mode of access: Internet.