634 resultados para Bruner, Jerome S.


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This report describes a tumor-associated antigen, termed CML66, initially cloned from a chronic myelogenous leukemia (CML) cDNA expression library. CML66 encodes a 583-aa protein with a molecular mass of 66 kDa and no significant homology to other known genes. CML66 gene is localized to human chromosome 8q23, but the function of this gene is unknown. CML66 is expressed in leukemias and a variety of solid tumor cell lines. When examined by Northern blot, expression in normal tissues was restricted to testis and heart, and no expression was found in hematopoietic tissues. When examined by quantitative reverse transcription–PCR, expression in CML cells was 1.5-fold higher than in normal peripheral blood mononuclear cells. The presence of CML66-specific antibody in patient serum was confirmed by Western blot and the development of high titer IgG antibody specific for CML66 correlated with immune induced remission of CML in a patient who received infusion of normal donor lymphocytes for treatment of relapse. CML66 antibody also was found in sera from 18–38% of patients with lung cancer, melanoma, and prostate cancer. These findings suggest that CML66 may be immunogenic in a wide variety of malignancies and may be a target for antigen-specific immunotherapy.

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We have cloned the Saccharomyces cerevisiae C-4 sterol methyl oxidase ERG25 gene. The sterol methyl oxidase performs the first of three enzymic steps required to remove the two C-4 methyl groups leading to cholesterol (animal), ergosterol (fungal), and stigmasterol (plant) biosynthesis. An ergosterol auxotroph, erg25, which fails to demethylate and concomitantly accumulates 4,4-dimethylzy-mosterol, was isolated after mutagenesis. A complementing clone consisting of a 1.35-kb Dra I fragment encoded a 309-amino acid polypeptide (calculated molecular mass, 36.48 kDa). The amino acid sequence shows a C-terminal endoplasmic reticulum retrieval signal KKXX and three histidine-rich clusters found in eukaryotic membrane desaturases and in a bacterial alkane hydroxylase and xylene monooxygenase. The sterol profile of an ERG25 disruptant was consistent with the erg25 allele obtained by mutagenesis.

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Unlike most normal adult tissues, cyclic growth and tissue remodeling occur within the uterine endometrium throughout the reproductive years. The matrix metalloproteinases (MMPs), a family of structurally related enzymes that degrade specific components of the extracellular matrix are thought to be the physiologically relevant mediators of extracellular matrix composition and turnover. Our laboratory has identified MMPs of the stromelysin family in the cycling human endometrium, implicating these enzymes in mediating the extensive remodeling that occurs in this tissue. While the stromelysins are expressed in vivo during proliferation-associated remodeling and menstruation-associated endometrial breakdown, none of the stromelysins are expressed during the progesterone-dominated secretory phase of the cycle. Our in vitro studies of isolated cell types have confirmed progesterone suppression of stromal MMPs, but a stromal-derived paracrine factor was found necessary for suppression of the epithelial-specific MMP matrilysin. In this report, we demonstrate that transforming growth factor beta (TGF-beta) is produced by endometrial stroma in response to progesterone and can suppress expression of epithelial matrilysin independent of progesterone. Additionally, we find that an antibody directed against the mammalian isoforms of TGF-beta abolishes progesterone suppression of matrilysin in stromal-epithelial cocultures, implicating TGF-beta as the principal mediator of matrilysin suppression in the human endometrium.

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En los estudios de la Diplomatura de Enfermería existe una división, tanto a nivel secuencial-cronológico (transmisión gradual y lógica de los contenidos), como en la dimensión espacio-ambiental (aula, sala simulación y servicio sanitario: comunitario u hospitalario). Estas divisiones tienen consecuencias en la estructura curricular de la disciplina: módulo teórico, módulo práctico y módulo práctico-clínico, confiriéndole a estos estudios una peculiaridad asentada en las diferencias en los tipos de escenarios, ambientes, instituciones, normas, y personas que se manifiestan en los contrastes percibidos (Merleau Ponty, 1988) por los alumnos en su proceso de enseñanza-aprendizaje según se dé éste en un módulo u otro. La enorme variabilidad de la práctica se ve también afectada por las características del escenario en el que tienen lugar las prácticas clínicas: centros de atención primaria y/o instituciones hospitalarias, en los que el alumno debe iniciar sus prácticas de enfermería con el que va a constituir su objeto-sujeto de trabajo durante toda su vida profesional: el ser humano (individuo, familia y comunidad) atendiendo a sus necesidades fisiológicas, psicológicas y sociales desde una perspectiva holística. Los contrastes entre el aula y el servicio sanitario suponen a veces contradicciones que desenfocan la visión lógica con la que el alumno pretende interpretar su realidad formativa en esta compleja fase de sus estudios, y esta visión borrosa puede derivar en situaciones de disminución o pérdida de significado que esta experiencia educativa debe tener en el alumno (Bruner, 1991).

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Manuscript notebook, possibly kept by Harvard students, containing 17th century English transcriptions of arithmetic and geometry texts, one of which is dated 1689-1690; 18th century transcriptions from John Ward’s “The Young Mathematician’s Guide”; and notes on physics lectures delivered by John Winthrop, the Hollis Professor of Mathematics and Natural Philosophy at Harvard from 1738 to 1779. The notebook also contains 18th century reading notes on Henry VIII, Tudor succession, and English history from Daniel Neal’s “The History of the Puritans and David Hume’s “History of England,” and notes on Ancient history, taken mainly from Charles Rollin’s “The Ancient History of the Egyptians, Carthaginians, Assyrians, Babylonians, Medes and Persians, Macedonians and Grecians.” Additionally included are an excerpt from Plutarch’s “Lives and transcriptions of three articles from “The Gentleman’s Magazine, and Historical Chronicle,” published in 1769: “A Critique on the Works of Ovid”; a book review of “A New Voyage to the West-Indies; and “Genuine Anecdotes of Celebrated Writers, &.” The flyleaf contains the inscription “Semper boni aliquid operis facito ut diabolus te semper inveniat occupatum,” a variation on a quote of Saint Jerome that translates approximately as “Always good to do some work so that the devil may always find you occupied.” In the seventeenth and eighteenth centuries, Harvard College undergraduates often copied academic texts and lecture notes into personal notebooks in place of printed textbooks. Winthrop used Ward’s textbook in his class, while the books of Hume, Neal, and Rollin were used in history courses taught at Harvard in the 18th century.

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Reviews of the sport psychology literature have identified a number of models of athlete development in sport (Alfermann & Stambulova, 2007; Durand-Bush &Salmela, 2001). However, minimal research has investigated the origins of knowledge from which each model was developed. The purpose of this study was to systematically examine the influential texts responsible for providing the basis of athlete development models in sport. A citation path analysis of the sport psychology literature was used to generate a knowledge development path of seven athlete development models in sport. The analysis identified influential texts and authors in the conceptualization of athlete development. The popula-tion of 229 texts (articles, books, book chapters) was selected in two phases. Phase1 texts were articles citing seven articles depicting models of athlete development(n  75). Phase 2 included texts cited three or more times by Phase 1 articles (n  154). The analysis revealed how the scholarship of Benjamin Bloom (1985) has been integrated into the field of sport psychology, and how two articles appearing in 1993 and 2003 helped shape present conceptualizations of athlete development

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Previous research has suggested that current formal coach education programs do not fully meet the learning needs of coaches. The purpose of the present study was to examine actual and preferred sources of coaching knowledge for developmental-level coaches. Structured quantitative interviews were conducted with coaches (N = 44) from a variety of sports. Learning by doing, interaction with coaching peers, and formal coach education were the top actual sources of coaching knowledge. Discrepancies were found between actual and preferred usage of learning by doing, formal coach education, and mentoring. Coaches indicated they would prefer more guided learning and less self-directed learning by doing. Further, differences in preferred sources were identified between coaches wishing to move to an elite level versus coaches wishing to stay at a developmental level. Findings highlight the importance of both experiential and formally guided sources of coaching knowledge and the context-specific nature of coach learning.

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Low concentrations of organic carbon in slowly accumulating sediments from Sites 597, 600, and 601 reflect a history of low marine productivity in the subtropical South Pacific since late Oligocene times. The distributions of n-alkanes, n-alkanoic acids, and n-alkanols provide evidence of the microbial alteration of sediment organic matter. Landderived hydrocarbons, possibly from eolian transport, dominate n-alkane distributions in these samples.

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Back Row: Coaches Bob Patek, Jerry Zuver, Mike Gittleson, Milan Vooletich, Dennis Brown, Jack Harbaugh, Bill McCartney, Jerry Hanlon, Don Nehlen, Tirrel Burton, Paul Schudel, Bob Thornbladh, Barry Pierson, Mike Smith, Curt Stephenson, Trainer Lindsy McLean, Eqp. Mgr. Jon Falk.

9th Row: Kevin Smith, Doug Agnew, * , Mike Butts, Steve Zarnata, Brad Fischer, Kevin Gilligan, * , Karl Tech, Jerome Jelinek, Bill Welch, * , Vince Shaw, Mgr. Nick Uriah

8th Row: Marion Body, Dave Brewster, Rich Strenger, Sanford Washington, Steve Reilly, Tom Neal, Norm Betts, Mike Petsch, Mike Lemirande, Gary Snell, Jeff Reeves, Tony Jackson, Jeff Felten, Tony Kelsie

7th Row: Mike Webster, Butch Woolfolk, Cedric Coles, Bubba Paris, Chuck Rowland, Ed Muransky, Mark Warth, Tom Garrity, Robert Thompson, Jim Paciorek, Gary Lee, Zeke Wallace, Brian Carpenter, John Sandberg

6th Row: Tom Moss, Tim Carrier, Jay Allen, Jim Breaugh, Larry Jones, David Angood, Tom Wandersleben, Fred Motley, Dave Payne, Rod Vaughn, Gasper Calindrino, Kevin Long, Bryan Virgil, *

5th Row: Brad Bates, Irvin Johnson, Kelly Keough, Tom Keller, Rick Novak, Ben Needham, Oliver Johnson, Jeff Jackson, Dan Kwiatkowski, John Prepolec, Greg Wunderli, Kurt Becker, Tony Osbun, Mike Kligis, Chuck Christian

4th Row: Craig Page, B.J. Dickey, Rodney Peaster, Dan Murray, Andy Cannavino, Dave Nicolau, Stanley Edwards, Michael Davis, Mike Trgovac, Stuart Harris, Roger Gaudette, Jim Kozlowski, Alan Mitchell, Rick Jones, Head Coach Bo Schembechler

3rd Row: Gerald Diggs, Tony Leoni, Roosevelt Smith, Gary Weber, Lawrence Reid, Mel Owens, George Lilja, John Powers, Chris Godfrey, John Wangler, Gene Bell, Michael Harden, Mike Leoni, Gary Quinn, Jim Humphries</p>

2nd Row: Gregg Willner, William Jackson, Mike Jolly, Ralph Clayton, Chip Pederson, Rock Lindsay, John Arbeznik, Ron Simpkins, Doug Marsh, Dale Keitz, Tom Melita, Mark Torzy, Tim Malinak, Ed Kasparek, Chuck Netts</p>

Front Row: Mark Braman, Mark DeSantis, Mark Schmerge, Curtis Greer, Greg Bartnick, Harlan Huckleby, Co-Captain Russell Davis, Bill Dufek, Rick Leach, Co-Captain Jerry Meter, Gene Johnson, Jon Giesler, Tom Seabron, Steve Nauta, Bob Hollway

* = left the team

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front row: Lloyd Carr. Jason Carr, Jay Riemersma, Mike Sullivan, Trent Zenkewicz, Joe Marinaro, Ed Davis, Jason Horn, Amani Toomer, Mercury Hayes, Jean-Agnus Charles, Todd Vander Least, Todd Richards.

2nd row: Charles Winters, Jarrett Irons, Steve King, Tyrone Noble, Remy Hamilton, Rod Payne, Jon Runyan, Thomas Guynes, Mark Bolach, Eric Wendt, Mike Vanderbeek, Woodrow Hankins, Deollo Anderson.

3rd row: Bryan Williams, Clarence Thompson, Tshimanga Biakabutuka, Earnest Sanders, Glen Steele, Damon Denson, Zach Adami, John Partchenko, Rob Swett, Brian Griese, Scot Loeffler, George Howell, Mike Hynes.

4th row: William Carr, Nate DeLong, Joe Ries, Brent Blackwell, Mike Elston, Ben Huff, Jeff Springer, Josh Cockrell, Sean Parini, Colby Keefer, Thomas Mondry, Matt De Young, Paul Peristeris.

5th row: Anthony Williams, Kraig Baker, Todd Brooks, Scott Dreisbach, Chris Howard, Chris Floyd, Mark Campbell, Rasheed Simmons, Jon Janson, Noah Parker, Chris Singletary, Sam Sword, Jerome Tuman, Clint Copenhaver, Juaquin Feazell.

5th row: Tyrone Butterfield, Marcus Ray, Andre Weathers, Jay Feely, Nate Miller, David Crispin, Matt Sygo, Terrance Quinn, Darren Petterson, Ed Kiser, Patrick Hill, Eric Mayes, Scott Parachek.

6th row Daydrion Taylor, Rob Renes, David Bowens, Tom Brady, Steve Frazier, DiAllo Johnson, Aaron Shea, Eric Moltane, Jeff Potts, Chris Ziemann, Ron Acheson, Pat Kratus,Tai Streets, Josh Williams, James Hall, Brent Washington, Charles Woodson.

7th row: Paul Barry, Harold Goodwin. Jason Cole, Brian Townsend, Jim Plocki, Joe Allore, Kevin Bryant, J.H. Ford, Clarence Williams, Unwana Ubom, Jeff Smokovitch, Kenneth Jackson, Tate Schanski, Bob Bland, Brian Hagens, Gordon Grace, Shomy Shembechler, Matt Hamilton.

8th row: Todd Jager, Paul Schmidt, Chad Brown, Mike Gittleson, John Milligan, Vance Bedford, Brady Hoke, Jim Herrmann, Greg Mattison, Mike DeBord, Fred Jackson, Bobby Morrison, Kit Cartwright, Erik Campbell, Scoff Draper, Jon Falk, Phil Bromley, Steve Connelley.