878 resultados para Wolan, Andrzej, 1530-1616.
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In this work, we report the findings of a study on scanning electrochemical microscopy (SECM) to investigate the interfacial electron-transfer (ET) reaction between the 7,7,8,8-tetracyanoquinodimethane radical anion (TCNQ(.-)) in 1,2-dichloroethane and ferricyanide in an ice-like matrix (a mixture of insulting ice and conductive liquid) under low temperatures. Experimental results indicate that the formed liquid/ice-like matrix interface is superficially similar in electrochemical characteristics to a liquid/liquid interface at temperatures above -20 degreesC. Furthermore, imaging data show that the surface of the ice-like matrix is microscopically flat and physically stable and can be applied as either a conductive or an insulting substrate for SECM studies. Perchlorate ion was selected as the common ion in both phases, the concentrations of which controlled the interfacial potential difference. The effect of perchlorate concentration in the DCE phase on interfacial reactions has been studied in detail. The apparent heterogeneous rate constants for TCNQ(.-) oxidation by Fe(CN)(6)(3-) in another phase under different temperatures have been calculated by a best-fit analysis, where the experimental approach curves are compared with the theoretically derived relationships. Reaction rate data obey Butler-Volmer formulation before and after the freezing point, which is similar to most other known cases of ET reactions at liquid/liquid interfaces. However, there is a sharp change observed for heterogeneous rate constants around the freezing point of the aqueous phase, which reflects the phase transition. At temperatures below -20 degreesC, surface-confined voltammograms for the reduction of ferricyanide were obtained, and the ice-like matrix became an insulating one, which indicates that the aqueous phase is really a frozen phase.
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Hydrotalcite-like compounds containing carbonate ion as the interlayer anion were prepared by coprecipitation under low supersaturation condition by mixing an aqueous solution of metal nitrates with an aqueous solutions of NaOH and Na2CO3, at room temperature, maintaining pH = 8-10 with vigorous stirring, Following the mixing, the resulting heavy slurry was aged at 353 K for 18 h with vigorous stirring, The precipitate was then filtered, washed several times with hot distilled water and dried in air at 353 K overnight, In this way, CuMI AlCO3-HTLcs and M-I AlCO3-HTLcs were synthesized and characterized by means of XRD and IR, The catalysis of the above mentioned HTLcs were investigated in the phenol hydroxylation with H2O2. The results indicated that all of the copper-containing HTLcs had a higher catalytic activity in the reaction, However, those catalysts that did not contain copper had no catalytic activity in this reaction, This means that copper was the active center in the phenol hydroxylation. Meanwhile, the mechanism was also proposed, which could be used to explain the main reason for higher activity for CuCuAlCO3-HTLcs in the phenol hydroxylation and the effect of Mg2+, Zn2+, Co2+, Ni2+ on activity of CuMI AlCO3-HTLcs.
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合成了一系列二元、三元过渡金属水滑石类化合物,并经XRD、IR进行了表征.苯酚过氧化氢羟化结果表明,含铜水滑石类化合物对该反应有较高的催化活性,并初步提出了反应机理.
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In addition to 10 known compounds (7-16), one new brominated diterpene, 10-hydroxykahukuene B (1), two new sesquiterpenes, 9-deoxyelatol (2) and isodactyloxene A (3), one new brominated C-15-acetogenin, laurenmariallene (4), and two new naturally occurring halogenated sesquiterpenes (5 and 6) that were previously obtained as intemediates in a biomimetic synthetic study of rhodolaureol and rhodolauradiol have been isolated and identified from the organic extract of the marine red alga Laurencia mariannensis. The structures of these compounds were established by spectroscopic methods. The antibacterial and antifungal activities of new compounds 1-4 were evaluated.
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alpha-葡萄糖苷酶是一类能够催化碳水化合物alpha-1,4-糖苷键水解的酶,是动物体内许多代谢途径中的关键酶,具有多方面的靶向药理活性。研究表明,alpha-葡萄糖苷酶与糖尿病,艾滋病,恶性肿瘤、溶酶体贮积症等疾病的发生机制有重要关系,已经成为治疗这些疾病的重要靶点。 海带(Laminaria japonica) 属褐藻门、海带属植物。自二十世纪四十年代从日本引入中国以来,已成为中国沿海重要的海洋经济作物。海带藻体叶片部分大都用来提炼碘、甘露醇和藻酸盐,但其假根部分一直被当作化工工业和食品工业的废料,而未得到有效的利用。在中国的沿海一带,海带根作为一种抗糖尿病的民间药物,有很长的历史。因此从海带根中提取分离降血糖成分并研究其作用机制将具有重要的意义。 本论文第一部分进行了海带根中具有alpha-葡萄糖苷酶抑制作用的活性组分的筛选。首先我们按照海带根中所含成分的极性不同进行了提取分离,即将海带根乙醇提取物依次用石油醚、乙酸乙酯、正丁醇萃取。结果乙醇粗体物、石油醚部位、乙酸乙酯部位、正丁醇部位与水部位的酶抑制IC50分别为1589µg/L、970µg/L、 380µg/L、1616µg/L、1106µg/L。乙酸乙酯部位的酶抑制活性最强。 本论文第二部分对海带根乙酸乙酯部位的提取物的体内降糖活性进行了研究,结果表明乙酸乙酯部位可以降低糖尿病大鼠餐后血糖,与阴性对照差异极显著(P<0.01)。小鼠服药后表现出的生命体征与伺服拜糖平后表现出的生命体征类似。 在以上工作基础上我们以alpha-葡萄糖苷酶的抑制活性为导向,用天然产物化学的方法对活性成分进行追踪,通过光谱、波谱与理化性质鉴定出了具有显著alpha-葡萄糖苷酶抑制活性的化合物:丁基-异丁基-邻苯酸二甲酯,并对其进行了酶抑制动力学研究。研究表明丁基-异丁基-邻苯酸二甲酯对alpha-葡萄糖苷酶表现为非竞争性抑制类型,IC50达到13.6μg/mL,且Km=2.39 g/L 本研究工作为进一步研究海带根降血糖成分的作用机理提供了研究基础。
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扇贝养殖是我国重要的海水养殖产业,然而自1997 年以来,养殖扇贝陆续爆发的大规模死亡,不但造成了巨大的经济损失,而且严重影响了该产业的健康发展。丝氨酸蛋白酶抑制因子及丝氨酸蛋白酶在无脊椎动物的免疫应答中起着核心作用,它们的协同作用直接导致外界病源入侵的信号转导和级联放大,并进一步激活一系列防御体系,如黑化反应、血液凝结和抗菌肽的合成等。因此,克隆扇贝参与免疫防御的丝氨酸蛋白酶抑制剂基因并对其功能进行研究,将有助于进一步研究扇贝的免疫防御机制,丰富和发展无脊椎动物免疫学的内容。 运用大规模EST技术和RACE技术从栉孔扇贝中克隆出一个Kazal型丝氨酸蛋白酶抑制剂基因,定名为CfKZSPI。该基因cDNA序列全长1788bp,其中5' 非编码区(Untranslated Region, UTR)为97 bp,3' UTR161 bp,有一个典型的多聚腺苷酸信号序列(AATAAA)和一个ploy A 尾巴,开放阅读框(Open Reading Frame, ORF)含有1530 bp,编码509 个氨基酸残基。对其推测氨基酸序列进行分析,发现其中包括22个氨基酸残基组成的信号肽序列和12个Kazal型丝氨酸蛋白酶抑制剂结构域。采用QRT-PCR(quantitative real time PCR)对鳗弧菌浸泡刺激后栉孔扇贝血淋巴中CfKZSPI 的 mRNA表达量进行了检测,发现其mRNA 的表达量在鳗弧菌刺激后3h明显上升,达到空白组的43.6倍;然后在6h时有所下降,为空白组的15.0倍;随着菌刺激时间的增长,CfKZSPI基因的 mRNA 表达量急剧增加,在刺激后8h,12h,24h分别达到空白组的174.1,207.8,675.4倍。统计分析发现3h(P=0.019<0.05)和12h(P=0.020<0.05)时,CfKZSPI基因mRNA表达量与空白组差异均显著。为了研究栉孔扇贝CfKZSPI的蛋白活性,将其第十二个结构域克隆到pET-32a(+)载体中,转化大肠杆菌Rosetta-gami(DE3)表达菌株,获得可溶性表达的蛋白rCfKZSPI-12,对其进行抑制蛋白酶活性的分析,发现其对胰蛋白酶有很强的抑制活性,而对凝血酶没有抑制活性。当rCfKZSPI-12与胰蛋白酶分子比率为1:1时,约90%的蛋白酶活性被抑制。运用狄更斯作图法研究rCfKZSPI-12对胰蛋白酶的抑制能力,结果发现其对胰蛋白酶的抑制常数为173 nmol L-1。 采用同样方法从海湾扇贝cDNA文库中克隆出一个Kunitz型丝氨酸蛋白酶抑制剂基因,定名为Aikunitz。该基因全长632 bp,其中5' UTR 为105 bp,3' UTR 为 245 bp,有一个典型的多聚腺苷酸信号序列(AATAAA)和一个ploy A 尾巴,ORF 含有282 bp,编码93 个氨基酸残基。推测的氨基酸序列N末端有一个20个氨基酸残基组成的信号肽序列,成熟蛋白包括一个Kunitz型丝氨酸蛋白酶抑制剂结构域。采用QRT-PCR对鳗弧菌和藤黄微球菌感染后海湾扇贝血淋巴中Aikunitz 的mRNA的表达量进行了检测,结果发现其在鳗弧菌刺激后3h到9h持续上升,9h时表达量为PBS对照组的4.49倍(P=0.008<0.05),然后开始下降,在72h时表达量为对照组的0.24倍(P=0.021<0.05);而在藤黄微球菌刺激后3h到12h其表达量上升,其中6h时为空白组的5.95倍(P=0.0004<0.01);12h以后迅速下降,其中24h的表达量为对照组的0.38倍(P=0.028<0.05)。将Aikunitz基因编码的成熟蛋白按照重组CfKZSPI-12的方法进行重组表达,并对重组蛋白进行抑制蛋白酶和抑菌活性分析。结果发现其对胰蛋白酶和弹性蛋白酶两种丝氨酸蛋白酶都没有抑制作用。抑菌实验同样发现,重组Aikunitz 对供试的革兰氏阳性菌藤黄微球菌和革兰氏阴性菌鳗弧菌和大肠杆菌都不显示明显抑菌活性。
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温度跃层是反映海洋温度场的重要物理特性指标,对水下通讯、潜艇活动及渔业养殖、捕捞等有重要影响。本文利用中国科学院海洋研究所“中国海洋科学数据库”在中国近海及西北太平洋(110ºE-140ºE,10ºN-40ºN)的多年历史资料(1930-2002年,510143站次),基于一种改进的温跃层判定方法,分析了该海域温跃层特征量的时空分布状况。同时利用Princeton Ocean Model(POM),对中国近海,特别是东南沿海的水文结构进行了模拟,研究了海洋水文环境对逆温跃层的影响。最后根据历史海温观测资料,利用EOF分解统计技术,提出了一种适于我国近海及毗邻海域,基于现场有限层实测海温数据,快速重构海洋水温垂直结构的统计预报方法,以达到对现场温跃层的快速估计。 历史资料分析结果表明,受太阳辐射和风应力的影响,20°N以北研究海域,温跃层季节变化明显,夏季温跃层最浅、最强,冬季相反,温跃层厚度的相位明显滞后于其他变量,其在春季最薄、秋季最厚。12月份到翌年3月份,渤、黄及东海西岸,呈无跃层结构,西北太平洋部分海域从1月到3月份,也基本无跃层结构。在黄海西和东岸以及台湾海峡附近的浅滩海域,由于风力搅拌和潮混合作用,温跃层出现概率常年较低。夏季,海水层化现象在近海陆架海域得到了加强,陆架海域温跃层强度季节性变化幅度(0.31°C/m)明显大于深水区(约0.05°C/m),而前者温跃层深度和厚度的季节性变化幅度小于后者。20°N以南研究海域,温跃层季节变化不明显。逆温跃层主要出现在冬、春季节(10月-翌年5月)。受长江冲淡水和台湾暖流的影响,东南沿海区域逆温跃层持续时间最长,出现概率最大,而在山东半岛北及东沿岸、朝鲜半岛西及北岸,逆温跃层消长过程似乎和黄海暖流有关。多温跃层结构常年出现于北赤道流及对马暖流区。在黑潮入侵黄、东、南海的区域,多温跃层呈现明显不同的季节变化。在黄海中部,春季多温跃层发生概率高于夏季和秋季,在东海西部,多跃层主要出现在夏季,在南海北部,冬季和春季多温跃层发生概率大于夏季和秋季。这些变化可能主要受海表面温度变化和风力驱动的表层流的影响。 利用Princeton Ocean Model(POM),对中国东南沿海逆温跃层结构进行了模拟,模拟结果显示,长江冲淡水的季节性变化以及夏季转向与实际结果符合较好,基本再现了渤、黄、东海海域主要的环流、温盐场以及逆温跃层的分布特征和季节变化。通过数值实验发现,若无长江、黄河淡水输入,则在整个研究海域基本无逆温跃层出现,因此陆源淡水可能是河口附近逆温跃层出现的基本因素之一。长江以及暖流(黑潮和台湾暖流)流量的增加,均可在不同程度上使逆温跃层出现概率及强度、深度和厚度增加,且暖流的影响更加明显。长江对东南沿海逆温跃层的出现,特别是秋季到冬季初期,有明显的影响,使长江口海域逆温跃层位置偏向东南。暖流对于中国东南沿海的逆温跃层结构,特别是初春时期,有较大影响,使长江口海域的逆温跃层位置向东北偏移。 通过对温跃层长期变化分析得出,黄海冷水团区域,夏季温跃层强度存在3.8年左右的年际变化及18.9年左右的年代际变化,此变化可能主要表现为对当年夏季和前冬东亚地区大气气温的热力响应。东海冷涡区域,夏季温跃层强度存在3.7年的年际变化,在El Nino年为正的强度异常,其可能主要受局地气旋式大气环流变异所影响。谱分析同时表明,该海域夏季温跃层强度还存在33.2年的年代际变化,上世纪70年代中期,温跃层强度由弱转强,而此变化可能与黑潮流量的年代际变化有关。 海洋水温垂直结构的统计预报结果显示,EOF分解的前四个主分量即能够解释原空间点温度距平总方差的95%以上,以海洋表层附近观测资料求解的特征系数推断温度垂直结构分布的结果最稳定。利用东海陆架区、南海深水区和台湾周边海域三个不同区域的实测CTD样本廓线资料,对重构模型的检验结果表明,重构与实测廓线的相关程度超过95%的置信水平。三个区重构与实测温度廓线值的平均误差分别为0.69℃,0.52℃,1.18℃,平均重构廓线误差小于平均气候偏差,统计模式可以很好的估算温度廓线垂直结构。东海陆架海区温度垂直重构廓线与CTD观测廓线获得的温跃层结果对比表明,重构温跃层上界、下界深度和强度的平均绝对误差分别为1.51m、1.36m和0.17℃/m,它们的平均相对误差分别为24.7%、8.9%和22.6%,虽然温跃层深度和强度的平均相对误差较大,但其绝对误差量值较小。而在南海海区,模型重构温跃层上界、下界和强度的平均绝对预报误差分别为4.1m、27.7m和0.007℃/m,它们的平均相对误差分别为16.1%、16.8%和9.5%,重构温跃层各特征值的平均相对误差都在20%以内。虽然南海区温跃层下界深度平均绝对预报误差较大,但相对于温跃层下界深度的空间尺度变化而言(平均温跃层下界深度为168m),平均相对误差仅为16.8%。因此说模型重构的温度廓线可以达到对我国陆架海域、深水区温跃层的较好估算。 基于对历史水文温度廓线观测资料的分析及自主温跃层统计预报模型,研制了实时可利用微机简单、快捷地进行温跃层估算及查询的可视化系统,这是迄今进行大范围海域温跃层统计与实时预报研究的较系统成果。
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目的:采用高效液相-蒸发光散射检测器(HPLC-ELSD)法测定藏药那保胶囊中黄芪甲苷的含量。方法:色谱柱为KromasilC_(18)柱(4.6mm×250mm,5μm);流动相为乙腈-水(36:64);ELSD(蒸发光散射检测器)检测。结果:黄芪甲苷在1-10μg范围内具良好的线性关系(r=0.999),平均回收率为99.05,RSD为2.48%。结论:该法简便可行,重复性好,适用于那保胶囊的质量控制。
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Reservoir prediction techniques from prestack seismic are among the most important ones for exploration of lithologic hydrocarbon reservoir. In this paper, we set the turbidite fan sandstone reservoir in Liao-Zhong depress as our researching target, and aims to solve the apllication difficulties on pre-stack inversion in the area, where the drilling data is scarce and the reservoir is lateral varied. Meanwhile, AVO analysis and pre-stack inversion for gas-bearing detection is systematically researched. The seismic reflection characters of gas-bearing sandstone in turbidite fan with different fluid content are defined, after analyzing results from AVO seismic simulation and porous fluid replacement of real log data, and under the guides of the seismic characters from classical gas-bearing sandstone reservoir and numerical simulation for complicate gas-bearing sandstone. It is confirmed that detecting gas-bearing sandstone in turbidite fan via AVO technologies is feasible. In terms of AVO analysis, two AVO characters, fluid detection factor and product of intercept and gradient, can effectively identify top and bottom boundaries and lateral range of tuibidite gas sand by comparing real drilling data. Cross-plotting of near and far angle stack data could avoid the correlation existing in P-G analysis. After comparing the acoustic impedance inversions with routine stacked data and AVO intercept, impedance derived from AVO intercept attribute could reduce the acoustic impedance estimating error which is caused by AVO. On the aspect of elastic impedance inversion, the AVO information in the pre-stack gathers is properly reserved by creating partial angle stack data. By the far angle elastic impedance alone, the gas sand, with abnormally low range of values, can be identified from the background rocks. The boundary of gas sand can also be clearly determined by cross-plotting of near and far angle elastic impedances. The accuracy of far angle elastic impedance is very sensitive to the parameter K, and by taking the statistical average of Vp/Vs on the targeted section in key wells, the accuracy of low frequency trends is gurranteed; the intensive absorsion within the area of the gas sand, which tends to push the spectral of seismic data to the lower end, will cause errors on the inversion result of elastic impedance. The solution is to confine the inversion on the interested area by improving the wavelet. On the aspect of prestack AVA simultaneous inversion, the constraint of local rock-physical trends between velocities of P-wave、S-wave and density successfully removes the instability of inversion, thus improves the precision of the resulting elastic parameters. Plenty of data on rock properties are derived via AVO analysis and prestack seismic data inversion. Based on them, the fluid anomaly is analysized and lithological interpretation are conducted. The distribution of gas sand can be consistently determined via various of ways, such as cross-plotting of P and G attributes, near and far partial angle stack data, near and far angle elastic impedances, λρ and Vp/Vs, etc. The shear modulo and density are also reliable enough to be used for lithological interpretation. We successfully applied the AVO analysis and pre-stack inversion techniques to gas detecting for turbidite fan sand reservoir in Liao-Zhong depression.
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Numerical modeling of groundwater is very important for understanding groundwater flow and solving hydrogeological problem. Today, groundwater studies require massive model cells and high calculation accuracy, which are beyond single-CPU computer’s capabilities. With the development of high performance parallel computing technologies, application of parallel computing method on numerical modeling of groundwater flow becomes necessary and important. Using parallel computing can improve the ability to resolve various hydro-geological and environmental problems. In this study, parallel computing method on two main types of modern parallel computer architecture, shared memory parallel systems and distributed shared memory parallel systems, are discussed. OpenMP and MPI (PETSc) are both used to parallelize the most widely used groundwater simulator, MODFLOW. Two parallel solvers, P-PCG and P-MODFLOW, were developed for MODFLOW. The parallelized MODFLOW was used to simulate regional groundwater flow in Beishan, Gansu Province, which is a potential high-level radioactive waste geological disposal area in China. 1. The OpenMP programming paradigm was used to parallelize the PCG (preconditioned conjugate-gradient method) solver, which is one of the main solver for MODFLOW. The parallel PCG solver, P-PCG, is verified using an 8-processor computer. Both the impact of compilers and different model domain sizes were considered in the numerical experiments. The largest test model has 1000 columns, 1000 rows and 1000 layers. Based on the timing results, execution times using the P-PCG solver are typically about 1.40 to 5.31 times faster than those using the serial one. In addition, the simulation results are the exact same as the original PCG solver, because the majority of serial codes were not changed. It is worth noting that this parallelizing approach reduces cost in terms of software maintenance because only a single source PCG solver code needs to be maintained in the MODFLOW source tree. 2. P-MODFLOW, a domain decomposition–based model implemented in a parallel computing environment is developed, which allows efficient simulation of a regional-scale groundwater flow. The basic approach partitions a large model domain into any number of sub-domains. Parallel processors are used to solve the model equations within each sub-domain. The use of domain decomposition method to achieve the MODFLOW program distributed shared memory parallel computing system will process the application of MODFLOW be extended to the fleet of the most popular systems, so that a large-scale simulation could take full advantage of hundreds or even thousands parallel processors. P-MODFLOW has a good parallel performance, with the maximum speedup of 18.32 (14 processors). Super linear speedups have been achieved in the parallel tests, indicating the efficiency and scalability of the code. Parallel program design, load balancing and full use of the PETSc were considered to achieve a highly efficient parallel program. 3. The characterization of regional ground water flow system is very important for high-level radioactive waste geological disposal. The Beishan area, located in northwestern Gansu Province, China, is selected as a potential site for disposal repository. The area includes about 80000 km2 and has complicated hydrogeological conditions, which greatly increase the computational effort of regional ground water flow models. In order to reduce computing time, parallel computing scheme was applied to regional ground water flow modeling. Models with over 10 million cells were used to simulate how the faults and different recharge conditions impact regional ground water flow pattern. The results of this study provide regional ground water flow information for the site characterization of the potential high-level radioactive waste disposal.
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对湖南香花岭锡多金属矿床成矿早、晚两期方解石进行了系统的C、O、Sr同位素研究。结果表明,成矿早期方解石具有相对较低的δ^13 CPDB(-5.4‰~-1.4‰)、δ^18 OSMOW(+6.1‰~+13.9‰)和较高的^87Sr/^86Sr值(0.7101~0.7230);而成矿晚期方解石则具有相对较高的δ^13 CPDB(+0.2‰~+0.6‰)、δ^18 OSMOW(+19.4‰~+21.5‰)和较低的^87Sr/^86Sr值(0.7101)。分析认为,成矿早期方解石为岩浆热液与海相碳酸盐岩相互作用的产物,成矿流体中的碳源于岩浆碳和海相碳酸盐岩;模拟计算结果显示,成矿早期方解石为成矿溶液发生0.05~0.1摩尔分数的CO2去气作用的产物。而成矿晚期方解石主要为矿区碳酸盐岩低温淋滤的产物,流体中的碳主要源于矿区碳酸盐岩围岩。成矿流体中的Sr源于矿区花岗岩和碳酸盐岩,从成矿早期到晚期,岩浆来源的Sr逐渐减少,而碳酸盐岩围岩提供的Sr比例不断增大。
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In Phys. Rev. Letters (73:2), Mantegna et al. conclude on the basis of Zipf rank frequency data that noncoding DNA sequence regions are more like natural languages than coding regions. We argue on the contrary that an empirical fit to Zipf"s "law" cannot be used as a criterion for similarity to natural languages. Although DNA is a presumably "organized system of signs" in Mandelbrot"s (1961) sense, and observation of statistical featurs of the sort presented in the Mantegna et al. paper does not shed light on the similarity between DNA's "gramar" and natural language grammars, just as the observation of exact Zipf-like behavior cannot distinguish between the underlying processes of tossing an M-sided die or a finite-state branching process.
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Q. Shen. Rough feature selection for intelligent classifiers. LNCS Transactions on Rough Sets, 7:244-255, 2007.
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Burnley, M, Doust, J and Jones, A (2005) Effects of Prior Warm-up Regime on Severe-Intensity Cycling Performance. Medicine and Science in Sports and Exercise, 37 (5). pp. 838-845. ISSN 1530-0315 RAE2008