Biodentine Reduces Tumor Necrosis Factor Alpha-induced TRPA1 Expression in Odontoblastlike Cells

INTRODUCTION: The transient receptor potential (TRP) ion channels have emerged as important cellular sensors in both neuronal and non-neuronal cells, with TRPA1 playing a central role in nociception and neurogenic inflammation. The functionality of TRP channels has been shown to be modulated by inflammatory cytokines. The aim of this study was to investigate the effect of inflammation on odontoblast TRPA1 expression and to determine the effect of Biodentine (Septodent, Paris, France) on inflammatory-induced TRPA1 expression.

METHODS: Immunohistochemistry was used to study TRPA1 expression in pulp tissue from healthy and carious human teeth. Pulp cells were differentiated to odontoblastlike cells in the presence of 2 mmol/L beta-glycerophosphate, and these cells were used in quantitative polymerase chain reaction, Western blotting, calcium imaging, and patch clamp studies.

RESULTS: Immunofluorescent staining revealed TRPA1 expression in odontoblast cell bodies and odontoblast processes, which was more intense in carious versus healthy teeth. TRPA1 gene expression was induced in cultured odontoblastlike cells by tumor necrosis factor alpha, and this expression was significantly reduced in the presence of Biodentine. The functionality of the TRPA1 channel was shown by calcium microfluorimetry and patch clamp recording, and our results showed a significant reduction in tumor necrosis factor alpha-induced TRPA1 responses after Biodentine treatment.

CONCLUSIONS: In conclusion, this study showed TRPA1 to be modulated by caries-induced inflammation and that Biodentine reduced TRPA1 expression and functional responses.

ACTITUDES HACIA LA VARIEDAD CANARIA : Un estudio sociolingüístico de estudiantes universitarios en Las Palmas de Gran Canaria.

En este estudio se analizan las actitudes lingüísticas hacia la variedad canaria en comparación con la variedad español peninsular considerado estándar de veinte estudiantes universitarios en Las Palmas de Gran Canaria. La investigadora utiliza una versión modificada del método matched guise que consiste en una grabación de cuatro versiones de un mismo texto leído por dos hablantes de la variedad canaria y dos hablantes de la variedad español peninsular considerado estándar. Los informantes escuchan el texto grabado y contestan consecutivamente un cuestionario, evaluando las voces de la grabación. Según el resultado obtenido existe una diferencia entre la valoración de la dimensión de estatus y la dimensión de solidaridad. Los informantes valoran más positivamente a la variedad español peninsular estándar al nivel estatus, mostrando actitudes desfavorables hacia la variedad canaria. En la dimensión de solidaridad se manifiestan actitudes contradictorias hacia la variedad canaria, indicando una posible oposición entre una variedad culta y una vulgar.

Lesões de cárie não cavitadas no esmalte

Tendo em conta os conceitos atuais da doença cárie dentária a necessidade de diagnóstico precoce das lesões de desmineralização que afetam o esmalte, pretendeu-se realizar uma revisão bibliográfica descritiva com os seguintes objetivos: descrever os principais conceitos acerca das lesões não cavitadas de cárie no esmalte, relacionadas com prevalência, gravidade, formas de deteção e registo; pretendeu-se ainda efetuar uma revisão da ação química dos agentes remineralizantes e infiltrantes, em lesões não cavitadas do esmalte, focando-se essencialmente na sua identificação, descrição, modos de apresentação, mecanismo de ação, modo de atuação clínica, principais evidências in vitro e in vivo sobre a ação dos remineralizantes e infiltrantes. Para tal, foi utilizada a metodologia PICO para a formulação das questões, avaliação e síntese da evidência empírica a incluir neste estudo. Os achados resultam da análise de 148 artigos, quer de perfil qualitativo, quer do perfil quantitativo, dos quais 104 são de revisões de literatura e 44 são empíricos, destes 44 artigos, 13 são relativos a infiltrantes e 31 são relativos a remineralizantes. Foram colocadas as palavras-chave: “enamel remineralization”, “ICDAS”, “white spot lesion”, “non-cavitated caries lesions”, “resin infiltration”, “infiltrants”, “dental caries detection”, “remineralizing agents”, “demineralization-remineralization” e “dental toothpaste”. Os critérios de inclusão foram: estudos observacionais, in vivo e in vitro, revisões narrativas, sistemáticas e meta-análises, escritas em nomenclatura Inglesa, sem período temporal definido, dando no entanto mais relevo clínico a publicações entre os anos de 2005 e 2016. Os critérios de exclusão foram todos os artigos que se referissem a lesões na dentina ou lesões cavitadas de esmalte, lesões odontopediátricas, lesões de cárie de raiz e materiais restauradores que não fossem infiltrantes. Foi possível concluir que ambas as técnicas (atuação por agentes remineralizantes e por infiltração resinosa) são eficazes na remineralização de lesões cariosas incipientes no esmalte. Os agentes remineralizantes apresentam uma vasta gama de formulações de acordo com as necessidades de cada paciente, enquanto os infiltrantes por serem uma técnica ainda recente apenas apresentam um composto disponível comercialmente para a sua aplicação em consultório dentário.

Yes, nós temos arquitetura moderna! Reconstituição e análise da arquitetura residencial moderna em Natal das décadas de 50 e 60

Brazilian architecture was recognized because of the consecration of the icons of the Carioca and Paulista schools which are represented nationally and internationally by names like Niemeyer, Lucio Costa and Vilanova Artigas, among others. Because of this, classic studies dedicated to the Brazilian case look to present the Southeastern region with the title of father of modern Brazil, at the cost of subjugating various other modern movements and peripheral sayings, whether their values are known or forgotten. On the other hand, there has been an effort, in the sense of registering and analyzing these regional productions of modern Brazilian architecture, an assignment that DOCOMOMO Brasil participates firmly through initiatives like the creation of a Library to aid in the documentation and registration of modernity in Brazil. Inside this context of insertions of the National-Modern scheme, this work has as its objective to present modern potiguar (northern Brazil) architecture through its contemporary residential examples, investigating specifically its constructive, formal aspects, that together that together demonstrate one more architectural emphasis of modern Brazilian architecture: the potiguar. This way, by contributing to the work of the register and the documentation of the Modern Movement and attributing to the modern architecture of Natal it s real worth, we can say: Yes, we have modern architecture

Neuromorphic model for sound source segregation

While humans can easily segregate and track a speaker's voice in a loud noisy environment, most modern speech recognition systems still perform poorly in loud background noise. The computational principles behind auditory source segregation in humans is not yet fully understood. In this dissertation, we develop a computational model for source segregation inspired by auditory processing in the brain. To support the key principles behind the computational model, we conduct a series of electro-encephalography experiments using both simple tone-based stimuli and more natural speech stimulus. Most source segregation algorithms utilize some form of prior information about the target speaker or use more than one simultaneous recording of the noisy speech mixtures. Other methods develop models on the noise characteristics. Source segregation of simultaneous speech mixtures with a single microphone recording and no knowledge of the target speaker is still a challenge. Using the principle of temporal coherence, we develop a novel computational model that exploits the difference in the temporal evolution of features that belong to different sources to perform unsupervised monaural source segregation. While using no prior information about the target speaker, this method can gracefully incorporate knowledge about the target speaker to further enhance the segregation.Through a series of EEG experiments we collect neurological evidence to support the principle behind the model. Aside from its unusual structure and computational innovations, the proposed model provides testable hypotheses of the physiological mechanisms of the remarkable perceptual ability of humans to segregate acoustic sources, and of its psychophysical manifestations in navigating complex sensory environments. Results from EEG experiments provide further insights into the assumptions behind the model and provide motivation for future single unit studies that can provide more direct evidence for the principle of temporal coherence.

Sistema de realidade aumentada no procedimento cirúrgico de remoção de pedras nos rins

A inovação tecnológica e as facilidades que gera tem tido um impacto crescente em diversas área, inclusivamente na medicina. A rápida evolução por parte de algumas tecnologias, como é o caso da Realidade Aumentada (RA), criam excelentes oportunidades, nomeadamente para intervenções cirúrgicas laparoscópicas, que apresentam especialmente problemas ao nível da exposição do doente a radiação. O presente documento detalha todo o processo de investigação e desenvolvimento realizado com a pretensão de criar um sistema de navegação por RA que auxilie o procedimento cirúrgico laparoscópico de remoção de pedras nos rins. Com este objetivo em perspetiva, e numa parceria com a empresa ECmedica LTD, foram desenvolvidos quatro protótipo funcionais. Com o intuito de compreender as melhores práticas de sistemas de input, interface e sistema de registo a aplicar, estes integraram aspetos inovadores tais como a utilização de uma sonda ultra-som, como substituta do raioX, e um registo feito através de sensores magnéticos. Apoiados numa metodologia de design centrado no utilizador e em instrumentos de análise como entrevistas e observação natural, os protótipos foram testados, obtendo respostas esclarecedoras relativamente ao objetivos dos protótipos. Foi observado que a RA é vista pelos médicos como uma solução com potencial, com as soluções apresentadas ao nível de inputs, interface e registo a serem bem recebidas. A projeção bidimensional oferecida pela imagem ultra-som foi encarada como insuficientes, sendo sugerida a sua substituição por um aumento tridimensional capaz de facilitar a correta inserção da agulha.

Environmental factors affecting maerl bed structure in Brittany (France)

This study used a large spatial scale approach in order to better quantify the relationships between maerl bed structure and a selection of potentially forcing physical factors. Data on maerl bed structure and morpho-sedimentary characteristics were obtained from recent oceanographic surveys using underwater video recording and grab sampling. Considering the difficulties in carrying out real-time monitoring of highly variable hydrodynamic and physicochemical factors, these were generated by three-dimensional numerical models with high spatial and temporal resolution. The BIOENV procedure indicated that variation in the percentage cover of thalli can best be explained (correlation = 0.76) by a combination of annual mean salinity, annual mean nitrate concentration and annual mean current velocity, while the variation in the proportion of living thalli can best be explained (correlation = 0.47) by a combination of depth and mud content. Linear relationships showed that the percentage cover of maerl thalli was positively correlated with nitrate concentration (R2 = 0.78, P < 0.01) and negatively correlated with salinity (R2 = 0.81, P < 0.01), suggesting a strong effect of estuarine discharge on maerl bed structure, and also negatively correlated with current velocity (R2 = 0.81, P < 0.01). When maerl beds were deeper than 10 m, the proportion of living thalli was always below 30% but when they were shallower than 10 m, it varied between 4 and 100%, and was negatively correlated with mud content (R2 = 0.53, P < 0.01). On the other hand, when mud content was below 10%, the proportion of living thalli showed a negative correlation with depth (R2 = 0.84, P < 0.01). This large spatial scale explanation of maerl bed heterogeneity provides a realistic physical characterization of these ecologically interesting benthic habitats and usable findings for their conservation and management.

Branching dendrites with resonant membrane: a "sum-over-trips" approach

Dendrites form the major components of neurons. They are complex branching structures that receive and process thousands of synaptic inputs from other neurons. It is well known that dendritic morphology plays an important role in the function of dendrites. Another important contribution to the response characteristics of a single neuron comes from the intrinsic resonant properties of dendritic membrane. In this paper we combine the effects of dendritic branching and resonant membrane dynamics by generalising the "sum-over-trips" approach [Abbott, L.F., Fahri, E., Gutmann, S.: The path integral for dendritic trees. Biological Cybernetics 66, 49--60 (1991)]. To illustrate how this formalism can shed light on the role of architecture and resonances in determining neuronal output we consider dual recording and reconstruction data from a rat CA1 hippocampal pyramidal cell. Specifically we explore the way in which an $I_{h}$ current contributes to a voltage overshoot at the soma.

A educação pré-escolar e a promoção da interculturalidade : o papel dos contos tradicionais

Relatório de Estágio apresentado à Escola Superior de Educação do Instituto Politécnico de Castelo Branco para cumprimento dos requisitos necessários à obtenção do grau de Mestre em Educação Pré-Escolar e Ensino do 1º Ciclo do Ensino Básico.

Organización financiera para la administración de fondos de las Asociaciones de Municipios, de la Zona Paracentral a investigarse en el periodo de mayo 2012 a mayo 2013

Esta investigación está dirigida a la Administración de Fondos de los diferentes tipos de Asociatividad Municipal: Asociación Municipal, Asociación de Municipios, Asociaciones de Municipalidades y Micro-Regiones de la Zona Paracentral. Los tipos de Asociatividad Municipal a los que se hace referencia en esta investigación administran recursos y obligaciones del sector público, por tanto son sujetos de derechos, atribuciones, y deberes propios, los que en conjunto conforman el ente contable gubernamental. Para el control, organización y registro de las diferentes transacciones de estas instituciones únicamente podrá realizarse en el Sistema de Contabilidad Gubernamental de los Entes (SICGE), aplicación informática proporcionada por el Ministerio de Hacienda. Para conocer el funcionamiento del control interno institucional se realizó un estudio a través de entrevista, encuestas y observación de los procesos, con el objeto de realizar un diagnóstico situacional sobre los procedimientos de controles aplicados. El control interno institucional fomenta la seguridad razonable en las cifras, oportunidad y consistencia en la información financiera, fundamental para la toma de decisiones. Con el objeto de ayudar al fortalecimiento institucional de las mancomunadas se incluye una propuesta de procedimientos de control interno financiero contables, con sus herramientas de control ofimatizadas, diseñados para la administración de los fondos de las Asociaciones de Municipios, que por ser entes contables, es preciso que cada mancomunidad cuente con su propio control interno financiero, que servirá para la transparencia de la gestión y la confiabilidad de la información financiera. EXECUTIVE SUMMARY This research is about at the Fund Management of different types of Municipal Associativity: Municipal Association, Association of Municipalities, Associations of Municipalities and Micro-Regions Paracentral Zone. Municipal Associative types referred to in this investigation about manage resources and obligations of the public sector, so they are subjects of rights, powers, and duties, which together make up the government accounting entity. For control, organization and registration of the several transactions of these institutions may only take place in the Government Accounting System of the Entities (SICGE) for acronym is spanish, computer application provided by the Ministry of Finance. For the functioning of the institutional internal control study was conducted through interviews, surveys and observation of processes in order to conduct a situational analysis on the procedure applied control. The Constitutional internal control promotes reasonable safety in numbers, timeliness and consistency financial information, critical for taking decision. In order to help the institutional strengthening of the asocitiation municipal is included a proposed of procedures of financial-accounting internal control, with its office automatization tools, which is designed for the administration of found the Association of Municipalities (political division of several cities), who is Gubernamental accounting entity, each association must have its own financial internal control, which will serve for management transparency and reliability of financial information.

Yes, nós temos arquitetura moderna! Reconstituição e análise da arquitetura residencial moderna em Natal das décadas de 50 e 60

Brazilian architecture was recognized because of the consecration of the icons of the Carioca and Paulista schools which are represented nationally and internationally by names like Niemeyer, Lucio Costa and Vilanova Artigas, among others. Because of this, classic studies dedicated to the Brazilian case look to present the Southeastern region with the title of father of modern Brazil, at the cost of subjugating various other modern movements and peripheral sayings, whether their values are known or forgotten. On the other hand, there has been an effort, in the sense of registering and analyzing these regional productions of modern Brazilian architecture, an assignment that DOCOMOMO Brasil participates firmly through initiatives like the creation of a Library to aid in the documentation and registration of modernity in Brazil. Inside this context of insertions of the National-Modern scheme, this work has as its objective to present modern potiguar (northern Brazil) architecture through its contemporary residential examples, investigating specifically its constructive, formal aspects, that together that together demonstrate one more architectural emphasis of modern Brazilian architecture: the potiguar. This way, by contributing to the work of the register and the documentation of the Modern Movement and attributing to the modern architecture of Natal it s real worth, we can say: Yes, we have modern architecture

Las relaciones entre el arte mural los espacios públicos y emblemáticos de la ciudad de Loja

La propuesta mural “Loja: Identidad Cultural” constituye una aspiración y requerimiento de la comunidad lojana, evidenciadas en el trabajo investigativo realizado en el Barrio Zamora de la ciudad de Loja, sector de la intersección de las calles José María Egas y Matilde Hidalgo de Procel, sobre el tema: “Las relaciones entre el arte mural, los espacios públicos y emblemáticos de la ciudad de Loja”. En el trabajo se pretende acercarse a la noción de espacio público con un recorrido por la historia que ayuda a dilucidar la diferencia entre lo público y lo privado. Así mismo, se advierte en las comunidades que las relaciones y acciones sociales, entre ellas el arte, constituyen elementos generadores de espacios propicios que fomentan el diálogo, la convivencia y la identidad. En uno de los capítulos se hace referencia al Muralismo como uno de los principales movimientos y corrientes de intervención del espacio público, sus orígenes, desarrollo y cómo se convirtió en una herramienta de poder político y de gestión en algunos países del mundo. También se realizó un breve estudio de los muralistas latinoamericanos, nacionales y locales, mediante un análisis comparativo y de registro de obras muralísticas que se encuentran en lugares públicos e importantes de la ciudad de Loja. La obra mural que fuera presentada y entregada a la comunidad lojana, en el acto público realizado el 18 de febrero del 2016 con la asistencia de representantes de las entidades seccionales, delegados de instituciones culturales, artistas plásticos, docentes y estudiantes de varios colegios de la ciudad; proyectó en su contenido el proceso histórico, aspectos culturales, personajes y elementos que buscan configurar la conciencia y la identidad lojana. La técnica que se utilizó en los 250 m2 es el acrílico y esmalte sobre ladrillo, mampostería y latón.

Technical performance profile of the four-time Costa Rican senior basketball league championship team

The purpose of this study was to analyze and compare the technical performance profile of the four-time Costa Rican Senior Basketball League championship team. A total of 142 games was recorded throughout the 2007, 2008 and 2009 seasons. Performance indicators selected were: two and three-point shots (converted, missed, effectiveness rates), free throws (converted, missed, effectiveness rates), points, offensive and defensive rebounds, fouls, turnovers, assists and ball steals. The information was described based on absolute and relative frequency values. Data was compared by season and by playing period based on the following non-parametric techniques: U-test, Friedman test and Chi-square. In all cases, SPSS version 15.0 was used with a significance level of p ≤ 0.05. Results showed a better profile of technical performance in the 2008 season, characterized by better percentages of two-point shots, free throws, fewer turnovers and more ball steals and assists. In relation to the playing period, the team showed a better technical performance profile during the second half of the matches. In general, the effectiveness rate of two-point shots and free throws was above 60% in both playing periods, while the three-point shot percentage ranged between 26.4% and 29.2%. In conclusion, the team showed a similar technical performance profile to that reported in the literature, as well as a clear evidence of the importance of recording and following up on technical performance indicators in basketball.

Por dentro da ordem : os comunistas na Assembleia Constituinte de 1946

Dissertação (mestrado)—Universidade de Brasília, Instituto de Ciências Humanas, Programa de Pós-Graduação em História, 2016.

Valeur diagnostique des micro-éveils autonomiques comme substitut aux éveils cérébraux dans l'apnée du sommeil

Les troubles respiratoires du sommeil ont une prévalence élevée dans la population générale, l’apnée obstructive du sommeil étant le plus important de ces troubles. Malgré tout, une grande proportion des patients avec apnée ne sont pas diagnostiqués. La méthode la plus complète pour diagnostiquer l’apnée est l’enregistrement d’une nuit de sommeil par polysomnographie, aussi appelée enregistrement de type 1, qui est un processus long et coûteux. Pour surmonter ces difficultés, des appareils d’enregistrements portables (ou de type 3) ont été développés. Toutefois, ces enregistrements de type 3 ne capturent pas l’activité cérébrale, mesurée avec l’électroencéphalographie (EEG). Le présent mémoire décrit une étude comparative entre les enregistrements de type 1 et de type 3. Tous les événements respiratoires d’apnée, d’hypopnée et d’éveils liés à un effort respiratoire (RERA, en anglais) seront analysés ainsi que les éveils cérébraux (ou éveils EEG) et les éveils autonomiques. Ces éveils autonomiques se définissent par une hausse de la fréquence cardiaque suite à un événement respiratoire. Pour enrichir les analyses, les variables respiratoires suivantes ont été étudiées : une chute de la saturation en oxygène de 4 % (ODI), l’index d’apnée-hypopnée (IAH), l’indice de perturbations respiratoires avec apnées + hypopnées + RERAs et les éveils EEG (RDIe, en anglais) et le RDI incluant les éveils autonomiques définis par une augmentation de la fréquence cardiaque de 5 bpm (RDIa5). L’objectif de la présente étude est d’évaluer la proportion d’événements respiratoires avec éveils autonomiques ainsi que leur impact sur le RDI des enregistrements de type 1 et 3. L’hypothèse suggère que les événements avec éveils autonomiques auraient un plus grand impact sur le RDI des enregistrements de type 3 contrairement au type 1. Cette étude inclut 72 sujets ayant suivi un enregistrement de polysomnographie complète de type 1 ainsi que 79 sujets différents ayant suivi un enregistrement ambulatoire de type 3. Les analyses suivantes ont été effectuées : 1) les pourcentages d’événements associés avec seulement des éveils autonomiques dans les enregistrements de type 1 et de type 3 ; 2) les fréquences de migration entre les catégories basses et élevées de sévérité de l’AHI en prenant en compte les événements associés avec seulement des éveils autonomiques ; 3) les Bland-Altman (B-A) pour mesurer l’accord entre l’AHI, le RDIe et le RDIa5 (type 1), et entre l’AHI et le RDIa5 (type 3) et ; 4) les corrélations de Pearson et les coefficients de corrélation intraclasse (ICC) pour mesurer l’accord entre l’AHI, le RDIe et le RDIa5 (type 1), et entre l’AHI et le RDIa5 (type 3). L’utilisation du critère de RDIa5 permet d’ajouter 49 % d’événements comptés avec l’AHI pour les enregistrements de type 1, et 51 % d’événements pour ceux de type 3. La présente étude montre que les éveils autonomiques ont un impact similaire autant pour le RDI des enregistrements de type 3 que de type 1. En conclusion, on peut affirmer que le RDIa5 est acceptable et fiable pour les enregistrements de type 3.