Fisiologia e formação de partículas lipídicas durante o crescimento da levedura Yarrowia lipolytica IMUFRJ 50682.

A levedura Yarrowia lipolytica tem sido muito investigada, especialmente por ser um microrganismo oleaginoso, ou seja, capaz de acumular grandes quantidades de lipídios, o que ocorre majoritariamente em organelas denominadas partículas lipídicas. Estes lipídios apresentam várias potenciais aplicações biotecnológicas, como por exemplo na produção de óleo microbiano (single cell oil) e na produção de biodiesel. Durante este projeto de mestrado, objetivou-se estudar a fisiologia de duas linhagens da levedura Y. lipolytica, sendo uma tradicionalmente estudada pela comunidade científica internacional (linhagem w29) e outra isolada da Baía da Guanabara, no Rio de Janeiro (linhagem IMUFRJ 50682). Foram realizados cultivos em frascos agitados tipo Erlenmeyer com defletores tampados com algodão (volume total 500 mL, volume de meio 100 mL, 28 oC e 200 rotações por minuto), durante os quais foi possível: 1) escolher um meio de cultivo de composição totalmente definida, com tiamina como único fator de crescimento, adequado a estudos de fisiologia quantitativa com esta levedura; 2) verificar que Y. lipolytica não é capaz de crescer com sacarose ou xilose como única fonte de carbono; 3) verificar que Y. lipolytica cresce com velocidade específica de crescimento máxima (Máx) de 0,49 h-1 num meio complexo contendo glicose, extrato de levedura e peptona (meio YPD), 0,31 h-1 em meio definido com glicose como única fonte de carbono e 0,35 h-1 no mesmo meio, mas com glicerol como única fonte de carbono, sem excreção de metabólitos para o meio de cultivo; 4) verificar que ocorreu limitação por oxigênio nos cultivos em frasco agitado, sendo este o motivo pelo qual as células deixaram de crescer exponencialmente; 5) verificar que o uso de ureia, em vez de sulfato de amônio, como fonte de nitrogênio, contribui para uma variação menor do pH durante os cultivos, sem prejuízo ao crescimento da levedura; 6) observar que, ao se restringir a oferta de nitrogênio à levedura (aumento da relação C/N inicial no meio de 12,6 para 126), as células têm sua morfologia alterada e apresentam maior quantidade de partículas lipídicas; 7) determinar uma composição elementar para a biomassa de Y. lipolytica (CH1,98O0,58N0,13), em que os átomos de carbono encontram-se em média mais reduzidos do que na biomassa de leveduras como Saccharomyces cerevisiae e Dekkera bruxellensis. Foram também realizados cultivos em biorreator em batelada (1 L de volume útil, 28 oC, aerobiose plena e pH controlado em 5,0), durante os quais foi possível: a) estabelecer um protocolo de cultivo para Y. lipolytica em biorreator (que envolvem agitação mecânica, aeração e uso de anti-espumante, entre outras diferenças em relação aos cultivos em frasco); b) confirmar os valores dos principais parâmetros fisiológicos apresentados por esta levedura, anteriormente obtidos a partir de cultivos em frasco; c) confirmar que o fator de conversão de substrato a células (Yx/s) é maior para cultivos realizados com glicerol como fonte única de carbono (0,53 g/g para a linhagem IMUFRJ 50682), do que com glicose (0,48 g/g para a mesma linhagem). Finalmente, cultivos realizados em quimiostato com glicerol como fonte de carbono e energia, limitados por amônio (fonte de nitrogênio, relação C/N 126), às vazões específicas de 0,25 h-1 e 0,15 h-1, permitiram observar que o número de partículas lipídicas por célula de Y. lipolytica permaneceu em torno de 2 em ambas as situações e houve uma diminuição no teor de nitrogênio nas células quando a velocidade específica de crescimento diminuiu de 0,25 para 0,15 h-1.

Fundamento del vigor y elegancia de la lengua castellana, expuesto en el propio y vario uso de sus partículas ... /

Mode of access: Internet.

PENSAR A CIBERCULTURA AMBIENTALISTA: Comunicação, mobilização e as estratégias discursivas do Greenpeace Brasil

Nossa Tese postula a existência de uma cibercultura ambientalista, própria do movimento ambiental, que conta com uma dinâmica comunicativa caracterizada por estratégias de discurso e mobilização específicas. O movimento ambiental, aqui representado pela organização de espectro internacional Greenpeace, soube se apropriar das ferramentas digitais, difundir a problemática em um cenário de redes sociais digitais, ciberativismo, interatividade e composição de uma esfera pública em rede, que colocamos em debate. Para entender esse panorama realizamos uma ampla discussão teórica, em permanente diálogo com nosso objeto de estudo, abrangendo a trajetória do ambientalismo e seu lugar enquanto movimento social; as tecnologias da sociabilidade, a Internet e suas mídias como espaço de resistência e controle, assinalando a cibercultura como a própria cultura contemporânea, pautada pelas influências tecnológicas. Realizamos entrevistas com voluntários, seguidores, além de responsáveis pela comunicação do Greenpeace que nos permitiram traçar as motivações da participação e confirmar que o engajamento na causa ambiental foi fortemente impulsionado pelas facilidades do ciberespaço. As estratégias discursivas foram desvendadas com as coordenadas metodológicas da Análise do Discurso, focada na identificação do ethos e das cenas de enunciação, com base em um protocolo de análise que formulamos para compreender a maneira de dizer que leva os sujeitos aderirem maciçamente ao discurso ambiental. Na primeira etapa da análise realizamos diagnóstico de perspectiva quantitativa e caráter exploratório para levantar as campanhas/temáticas principais e avaliar a repercussão dos assuntos nas redes sociais digitais e na mídia convencional. Posteriormente, selecionamos os textos das principais campanhas que passaram pela fase qualitativa, que abarcou os itens lexicais, as técnicas argumentativas e os elementos de destacabilidade, além de aspectos externos ao texto linguístico, como fotos, vídeos, cores e cenas predominantes. O discurso na cibercultura ambiental desvela o ethos do amigo, do parceiro, que oscila entre o drama e a agressividade para chamar atenção à causa. Problemas graves como denúncias ambientais são tratados com um ethos lúdico, até mesmo infantil, usando de linguagem coloquial e de códigos da cultura contemporânea desenhos animados, jogos virtuais, belos animais que cantam e dançam que para os nossos olhos revelam uma cenografia esquizofrênica, mas é justamente o que garante o êxito das campanhas.

Experiências subjetivadoras no limite da pele: Uma análise de práticas discursivas inscritas em tatuagens

This research aims to study the constitution of subjectivities in discursive practices included in imagistic and verbal texts of tattoos. Seeks to grasp the effects of meanings which translate emotions, experiences and disaffection that marked and / or transformed the lives of tattooed subjects. This thesis was anchored in Foucault's theories (1990, 2010, 2012) that address ways of subjectivity from the writing itself, transgression technologies as overshoot, opening new possibilities for discursive subject producing care of itself. Still notions from this analysis of French discourse (AD), as interdiscourse and discursive memory are used. The nature of qualitative research intends to contribute to the understanding of discourse as social practice constitutive of meaning in ways of being of the subject, as well as production of discourses about social norms. The corpus is composed of testimonials and imagistic and verbal texts of five subjects, among the ten respondents who tattooed experiences in the skin. The results show data demonstrating the constitution itself through lived experiences and printed on the skin. The meanings generated in the skin are types of subjectivities that reflect happiness, resilience, protection and immortality. We conclude that our subjects are positioned discursively in order to rebuild their bodies and experiences as necessary and that the images and statements recorded in bodies produce effects felt around the reflection about the ways of life and the existence choices of each one of the tattooed subjects.

Representações discursivas de Câmara Cascudo por Mário de Andrade

This piece of research aims to address the issue on the construction and reconstruction of Câmara Cascudo´s discursive representations in Mário de Andrade´s discourse. In order to describe, analyze and interpret these representations, we recur to some semantic categories from the Discourse Textual Analysis (DTA) by articulating them with other categories, notably, Grize´s Logic (1996,1997), Text Linguistics and Semantics. Therefore, the purpose is to analyze how these representations are constructed discursively, in written letters, by means of semantic categories such as referentiation, predication, modification, connection and spatial and temporal locations. In the theoretical foundation, proposals of Textual Analysis of Discourses, conceived by the linguist Jean-Michel Adam (1990, 2008a, 2011a), Text Linguistics, Semantics and Logic, focusing, especially, on the phenomenon of discursive representations, are articulated. The research approach is of qualitative nature supported by some quantitative data (OLIVEIRA, 2012); option which makes the analysis richer and comprehensive. As a hypothesis, it is presented the fact that these categories used by Mário de Andrade, in his discourse, do not only enable the (re)construction of the interlocutor´s images, discursively constructed, but they also provide a multiplicity of information and viewpoints about the RN writer´s personality. The study corpus is constituted of 20 texts written by Mário de Andrade and sent to Câmara Cascudo between 1924 and 1944, of which 35 fragments were selected and analyzed. However, it can be verified that, in the analyzed corpus, a set of discursive representations is constructed for Câmara Cascudo, from semantic categories proposed for analysis and used in Mário de Andrade´s discourse. These categories enable to construct and reconstruct the representations that emerge in the texts. Therefore, the analysis points out the construction of a set of different representations, highlighting the representation of the writer, the intellectual and the friend.

Análise textual das representações discursivas no discurso político brasileiro: o discurso da primeira posse da presidenta Dilma Rousseff (1°/01/2011)

Cette recherche décrit la représentation discursive que la présidente Dilma Rousseff fait de soi-même, dans son discours devant le Congrès National, lors de la journée d’investiture, le 1/1/2011. Notre travail se situe dans le domaine théorique et méthodologique de la linguistique du texte et, plus spécialement, dans la perspective de l’Analyse Textuelle des Discours – ATD (ADAM, 2011 [2008a]), qui se définit comme « une théorie de la production co(n)textuelle de sens qui doit se fonder sur l’analyse de textes concrets ». Elle nous fournit la notion théorique et analytique de « représentation discursive », qui se focalise sur la dimension sémantique du texte. Nous nous appuyons, aussi, sur des travaux récents sur les représentations discursives, réalisés dans la recherche brésilienne sur l’ATD (RODRIGUES, PASSEGGI, SILVA NETO, 2010, 2012; RAMOS, 2011; OLIVEIRA, 2013; QUEIROZ, 2013; ZAMBLANO-OLIVEIRA, PASSEGGI, 2013). Les principales opérations sémantiques de construction de la représentation discursive utilisées dans notre travail sont la Référenciation et la Prédication. L’approche méthodologique est, en même temps, qualitative et quantitative, priorisant l’identification des occurrences, ainsi que la description détaillée de leurs valeurs sémantiques et textuelles. Les résultats de la recherche sont de trois ordres : méthodologique, théorique et descriptif-interprétatif. Méthodologique : nous proposons une approche que nous désignons comme « marquage textuel » (ou « mappage textuel ») qui permet de marquer (étiqueter) les valeurs sémantiques des formes linguistiques, permettant leur identification dans le flux textuel, c.-à-d., dans la dimension séquentiellecompositionnelle du texte. Théorique : nous introduisons la notion de « domaines de la représentation discursive » qui organisent et articulent les différents éléments qui composent la représentation discursive de la présidente. Quant aux résultats descriptifsinterprétatifs du discours de l’investiture, ils indiquent que la représentation discursive de la présidente se configure au moyen de différents domaines conceptuels, explicités par les référenciations et les prédications. Ils mettent en relief les désignations et les actions / états, aussi bien de la femme – domaine du genre – que de la présidente – domaine du rôle politique et institutionnel. La présidente se représente explicitement et avec emphase comme l’agent responsable par les actions exprimées par les prédications verbales (verbes d’action), consciente de l’importance de son rôle politique et social. Les prédications nominales signalent clairement une représentation discursive qui englobe les domaines conceptuels politique, moral, éthique, comportemental et émotionnel (forte, accueillante, pionnière, consolidatrice, infatigable, humble, engagée, démocrate, victorieuse et courageuse). Le discours d’investiture réalise, donc, des désignations positives de la présidente, lesquelles se situent dans un temps présent et prospectif – avec des perspectives de futur – comme leader politique du Brésil, avec une participation active dans la transformation du pays, tenant compte aussi de son histoire de vie, sa biographie de luttes. Ainsi, la description empirique et l’interprétation de ce discours particulier contribue à l’analyse textuelle des représentations discursives dans le discours politique brésilien contemporain. Elle soulève, aussi, des questions théoriques et méthodologiques qui nous semblent pertinentes pour le développement de cette aproche.

Representações discursivas de Lula nas capas das revistas Época e Veja

This doctoral dissertation proposes to analyze the discursive representations of Lula, as they appear on the covers of the magazines, Época and Veja, targeting the verbo visual elements that comprise the genre, magazine covers. In this way, we seek to describe and interpret the discursive representations (Drs), using a theoretical framework based on the Textual Discourse Analysis –TDA, developed by Jean Michel Adam (2011a), focusing on the semantic level of the text, that is, on the dimension that allows for the comprehension of Drs present in a text. For a discussion about the Drs and their categories of analysis referencing, predication, modification, relation and spatial localization and time we use as a starting point, the study by Brize about the logical discursive operations (1990, 1996), and continue through the studies that discuss linguistic, textual, and discursive operations in concrete utterances, such as Castilho (2010), Rodrigues; Passeggi; Silva Neto (2010), Neves (2011), Rodrigues et al. (2012), Passeggi (2001; 2012), Queiroz (2013), among others. In addition, we rely on Multimodal Discourse for the verbo visual aspects present on magazine covers (KRESS; van LEEUWEN, 2006; DIONISIO, 2011; DIONISIO; VASCONCELOS, 2013). Using aresearch approach that is qualitative with quantitative support, and which is documental, and based on deductive inductive methods, we describe and interpret a corpus (SEVERINO, 2007; CHIZZOTTI, 2010; OLIVEIRA, M., 2013), aiming to reconstruct Lula’s Drs. The cor pusis comprised of forty one magazine covers sixteen from Época and twenty four from Veja. The covers date from the election period in which the candidate, Lula, was elected President of Brazil in 2002, the last mandate after his reelection in 2006, and in the year 2010 a period of 9 years. Based on the analysis carried out, we can affirm that the magazines, Época and Veja, construct diverse Drs by Lula, such as: candidate; elected candidate; governing member and member of a political party; reelected president; politics; workers party acronym PT; international governments as allies; accomplices and participants in scandals of corruption; friend, brother, cousin, nephew, father, parent and man; among others that unfold throughout these by the mediation of the modifiers of the referents and processes, and by the very processes and connections, and analogies made on the object of discourse, Lula. Nonetheless, the reconstruction of these is derived from the description and interpretation of the textual linguistic and discursive choices that the magazines make to produce the proposition utterances, as well as by the choices of images and other visual resources, all operating as co(n)textually articulated to produce the magazine’s desired effect. In conclusion, the Drs verified demand the reflection, description and interpretation of the referencing, prediction, the relationship and spatial temporal localization, which was only possible through the textual discursive analysis of the verbo visual arrangements that comprise texts in the genre magazine cover.

Operações linguístico-discursivas em relatos retextualizados por alunos do ensino fundamental

The following paper attempted to investigate and discuss the possible improvements in the writing of students from the public network in the 6th grade of elementary school. This research was run in the professional master degree In Portuguese Language. Also based upon Genetic Criticism, this study attempting to analyze in which way and in which proportions the alterations done in the texts reveal and perspective capacity in the students as they replace, broad, remove or just move terms in their assignments. We tried to develop our analysis in the pragmatic perspective in which Textual Linguistic is included, investigating the texts and appreciating them as a construction process of meaning. Our theoretical discussion will be based on and socio-interactional conception of language (MARCUSCHI, 2008), as well as in the postulates of Analyze of Discursion (ADAM, 2010, 2008). It has also been used the theoretical assumptions taken from Genetic Criticism which regard the relation between text and genesis, due to the fact that it considers the text and a the result of a construction of progressive elaboration and the writing as an activity of constant movement (DE BIASI, [2000] 2010; GRÉSILLON, 1989; [1990] 2008; [1992] 2002; SALLES, 2008a). To assemble the data in this research we took into consideration the social variables related to the students and the sociocultural context. Nonetheless, this investigation point towards a real classroom situation where we tried to analyze the language functioning in application conditions, thus, giving us authority to describe more precisely the reality we lived in, which provided us with a more attentive look to the observed particularities. This description of the reality appreciated built a path that point to a research of qualitative data approach to which meanings deriving from interpretation were attributed. To conduct this research we resourced to the Action-Research process. The data are comprised of ten personal reports that were created from rewriting assignments, which constitutes a range of twenty texts assessed starting from the linguistic operations acknowledged by Generative Grammar and continued by Lebrave and Grésillon (2009). As result of this analysis, we identified the operation of adding more often than the other ones. The replacement and removal operations display a very close offspring. Nevertheless, the moving operation was scarcely used. These results, besides demonstrating linguistic creativity of the students, revealed that for a text to be seen as “concluded”, the writing student articulates new elaborations through these linguistic operators and that these movements of coming and going, of erasures and amendments contribute significantly for the teacher to approach the relationship the student keeps with his textual and discursive expression, and then, with that, to hold information that eventually provides individual and collective directions in school productions.

Representações discursivas sobre lampião e seu bando em notícias de jornais mossoroenses (1927): “O mais audaz e miserável de todos os bandidos” e o seu “grupo de asseclas”

In this doctoral thesis analyzed the discursive representations of the bandit Lampião, the Lantern and his bandits gang in news mossoroenses newspapers published in the twenties of the last century (1927), when the gang invasion of the city of Mossoro in the state of Rio Grande do Norte, on June 13 of that year. To this end, we take as basis the theoretical assumptions of linguistics Textual, especially the narrower context of what is known today as Textual Analysis of the Discourses (ADT), theoretical and descriptive approach to linguistic studies of the text proposed by the French linguist Jean-Michel Adam. In this approach, we are interested in, specifically, the semantic level of the text, highlighting the notion of discursive representation, studied based on benchmarking operations, predication, modification, spatial location and temporal connection and analogy (ADAM, 2011; CASTILHO, 2010; KOCH, 2002, 2006; MARCUSCHI, 1998, 2008; NEVES, 2007; RODRIGUES, PASSEGGI & SILVA NETO, 2010). The corpus of this research consists of three reports in the twenties of the last century in newspapers The Mossoroense, Correio do Povo and the Northeast, and reconstituted through the collection held in the Municipal Museum Lauro Scotland files, Memorial Resistance Mossoro, both located in Natal, and in the news collection of Lampião newspapers in Natal, north of Rio Grande Raimundo Nonato historian. The discursive representations are built from the use of semantic analysis operations. Lampião to, the following representations are built: bandit, head of bandits, briber, defeated, Captain and Lord. To the outlaws of Lampião bunch of the following discursive representations were built: group, gang, gangsters, mates, bloodthirsty pack, brigands, bandits, criminals, burglar horde, and wild beasts. These representations reveal mainly the views of the newspapers of that time, which represented mainly the interests of traders, politicians, the government itself and generally Mossoró population.

Practicas discursivas sobre el cuerpo en la Universidad de los Llanos

El estudio aquí presentado intentó comprender las concepciones que de cuerpo subyace en la formación de maestros del programa de Licenciatura en Educación Física y Deportes de la Universidad de los Llanos, y cómo estos se develaron en el currículo. Para dicho análisis se acudió a la aplicación de una metodología que permitiera vislumbrar la tendencia y la agenda investigativa de docentes, para prospectar con objetividad dicha situación. Se debió, entonces, abordar la metodología del rastreo arqueológico a partir de la descripción de las condiciones conceptuales del saber objeto de estudio. Los resultados se antojan inquietantes e interesantes en tanto revelación documental partiendo de ciertas categorías de análisis que da cuenta del trasegar del cuerpo en el currículo a partir de 1998