Restauração transcirúrgica como alternativa na resolução das perfurações endodônticas

The endodontic perforations are iatrogenic communications that result in a communication between the endodonto and periodontium. This occurrence represents about 10 % of cas- es of failure in endodontic treatment. The management may vary and is related to the drilling site endodontic. This paper aims to report a case of iatrogenic perforation and one of the possible alternatives for the treatment of endodontic perforations -level cervical tooth. During the opening coronary perforation occurred due to improper slope cervical penetration drill. Considering the drilling site, we opted for sealing com- posite resin after surgical access and absolute isolation. After sealing, the channel was filled with medication and instru- mented based on calcium hydroxide. Later the canal was obturated by lateral condensation technique with Sealapex. The radiographic follow-up showed regression of the lesion and the clinical silence confirmed the success of the treatment.

Avaliação da incorporação de enxerto osseo homogeno fresco congelado em humanos: análise histologica e imuno-histoquímica

Allogeneic, fresh-frozen bone has been used in order to replace bone autografts. However, its osteoinduction and osteoconduction properties are not well-defined in the scientific literature. This work aimed to evaluate samples of homogenous bone grafts in humans by qualitative histological and immunohistochemical analysis. For this, ten pre-selected patients underwent surgical augmentation of bone defects. The homogenous fresh frozen block bone graft was stabilized and fixed by bicortical screws. After six months, the reopening procedure was performed for installation of osseointegrated implants. At this time surgical bone graft samples were removed by means of drill trephine. The samples were fixed in 10% formalin, processed with decalcified paraffin, and stained with hematoxylin and eosin. Immunohistochemistry was performed for the expression of Caspase 3 enzyme. The slides were brought to light microscopy for qualitative histology and immunohistochemistry. The results showed non-vital bone tissue, with few areas of deposition of new bone formation on the amorphous matrix, presence of chronic inflammatory infiltrate with areas of osteomyelitis, and expressive immunolabeling of Caspase 3. Given the methods employed and the results it was concluded that the allograft fresh-frozen block is not incorporated into the recipient bed after a healing period of six months.

Modelamento matemático para otimização de perfuração em seções de pré-pal: um foco em operações no oceano Atlântico sul

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Caracterização morfocultural e molecular de isolados de Colletotrichum spp. provenientes de diferentes frutas tropicais

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Importância econômica e generalidades para o controle de Telchin licus Drury, 1773 (Lepidoptera: Casttniidae) em cana-de-açúcar

The sugar cane plantation expands its borders each year, throughout the national territory. Thus, increases the amount of biomass that will to be exploited by man in sugar and alcohol produce and also by other organisms, which will have food in abundance. The growth of mechanized harvesting, with the consequent decrease in burning of straw and the expansion of the sucroalcooleiro sector are causing changes into entomofauna in certain areas or regions of sugar cane plantation. One of the new threats to the sugar cane plantations in southcentral region, causing uncertainty and concern to farmers, is the giant worm, Telchin licus, known in Brazil since 1927, in the Northeast of Brazil, is considered a major pest of cane sugar. In 2007 it was first recorded in the state of São Paulo, which accounts for 60% of the country's crops. Whereas until then there is not much information about their management and control, the aim of this review is to gather information on the basis for its control within the context of Integrated Pest Management of cane sugar.

Modelos de previsão de ocorrência de adultos de diatraea saccharalis (Fabricius, 1794) (Lepidoptera: Crambidae) em cana-de-açúcar (Saccharum spp.)

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Avaliação in vitro do tempo de fotopolimerização com led de alta potência de bráquetes ortodônticos e remoção de adesivo remanescente sob luz ultravioleta

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Caracterização e comparação de Cotesia flavipes (Hymenoptera: braconidae) em situação de criação massal utilizando microssatélites e morfologia

Pós-graduação em Agronomia (Entomologia Agrícola) - FCAV

Morfologia do testículo e espermatogênese em Telchin licus (Drury, 1773) (Lepidoptera: Castiniidae)

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Morfologia do testículo e espermatogênese em Telchin licus (Drury, 1773) (Lepidoptera: Castiniidae)

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

The medial amygdaloid nucleus is involved in the cardiovascular pathway activated by noradrenaline into the lateral septal area of rats

We have previously reported that noradrenaline (NA) microinjected into the lateral septal area (LSA) caused pressor and bradicardic responses that were mediated by vasopressin release into the circulation through the paraventricular nucleus of hypothalamus (PVN). Although PVN is the final structure involved in the cardiovascular responses caused by NA in the LSA, there is no evidence of direct connections between these areas, suggesting that some structures could be links in this pathway. In the present study, we verified the effect of reversible synaptic inactivation of the medial amygdaloid nucleus (MeA), bed nucleus of stria terminalis (BNST) or diagonal band of Broca (DBB) with Cobalt Chloride (CoCl2) on the cardiovascular response to NA microinjection into the LSA of unanesthetized rats. Male Wistar rats had guide cannulae implanted into the LSA and the MeA, BNST or DBB for drug administration, and a femoral catheter for blood pressure and heart rate recordings. Local microinjection of CoCl2 (1 mm in 100 nL) into the MeA significantly reduced the pressor and bradycardic responses caused by NA microinjection (21 nmol in 200 nL) into the LSA. In contrast, microinjection of CoCl2 into the BNST or DBB did not change the cardiovascular responses to NA into the LSA. The results indicate that synapses within the MeA, but not in BNST or DBB, are involved in the cardiovascular pathway activated by NA microinjection into the LSA.

Agronomic characterization of banana mutants obtained by gamma radiation

Banana is one of the most economically important fruit, explored almost exclusively by small producers as a continuous source of food and income. Although Brazil is one of the main banana producers, the national banana production is undergoing serious problems especially in the phases of production and post-harvest limiting its participation in the international market. One of the main factors leading to great production losses is the toppling over due to the tall height of plants of main commercial cultivars. A strategy to solve this problem is reducing height by inducing mutation. The objective of the present work was to characterize irradiated Prata type banana mutants (cvs. Pacovan and Preciosa) during two production cycles in order to select short plants in height with good agronomic characteristics. In vitro plants of both cultivars were irradiated with gamma rays in the doses of 20 Gy ('Pacovan', 200 plants) and 30 Gy ( 'Preciosa', 200 plants) subcultivated four times and afterwards evaluated in the field during two production cycles. Four possible mutants were selected from each cultivar with height smaller than the average height of the controls after two evaluation cycles. It was observed that some of these mutants presented greater precocity and bunch weight compared to the controls. From the results obtained it is possible to select mutant plants with superior agronomic characteristics for 'Pacovan' as well as 'Preciosa' submitted to gamma radiation.

Brain Pathways Involved in the Modulatory Effects of Noradrenaline in Lateral Septal Area on Cardiovascular Responses

We have previously reported that stimulation of alpha-1 adrenoceptors by noradrenaline (NA) injected into the lateral septal area (LSA) of anaesthetized rats causes pressor and bradycardic responses that are mediated by acute vasopressin release into the circulation through activation of the paraventricular nucleus (PVN). Although the PVN is the final structure of this pathway, the LSA has no direct connections with the PVN, suggesting that other structures may connect these areas. To address this issue, the present study employed c-Fos immunohistochemistry to investigate changes caused by NA microinjection into the LSA in neuronal activation in brain structures related to systemic vasopressin release. NA microinjected in the LSA caused pressor and bradycardic responses, which were blocked by intraseptal administration of alpha-1 adrenoceptor antagonist (WB4101, 10 nmol/200 nL) or systemic V-1 receptor antagonist (dTyr(CH2)5(Me)AVP, 50 mu g/kg). NA also increased c-Fos immunoreactivity in the prelimbic cortex (PL), infralimbic cortex (IL), dorsomedial periaqueductal gray (dmPAG), bed nucleus of the stria terminalis (BNST), PVN, and medial amygdala (MeA). No differences in the diagonal band of Broca, cingulate cortex, and dorsolateral periaqueductal gray (dlPAG) were found. Systemic administration of the vasopressin receptor antagonist dTyr AVP (CH2)5(Me) did not change the increase in c-Fos expression induced by intra-septal NA. This latter effect, however, was prevented by local injection of the alpha-1 adrenoceptor antagonist WB4101. These results suggest that areas such as the PL, IL, dmPAG, BNST, MeA, and PVN could be part of a circuit responsible for vasopressin release after activation of alpha-1 adrenoceptors in the LSA.

Influência do preparo do orifício piloto na ancoragem dos parafusos pediculares

OBJETIVO: Avaliamos a influência do diâmetro e do modo de preparação do orifício piloto na resistência ao arrancamento e no torque de inserção dos parafusos pediculares com diâmetro interno cônico. MÉTODOS: Ensaios mecânicos foram realizados com parafusos pediculares com alma cônica e diâmetro de 4,2mm e 5,2mm inseridos nos pedículos vertebrais lombares de suínos. O orifício piloto foi confeccionado com de broca e sondas (pontiaguda e cortante) com diferentes diâmetros. RESULTADOS: Testando o parafuso de 4,2mm a perfuração com orifício igual ou inferior ao menor diâmetro interno do parafuso, aumentou o torque e a resistência ao arrancamento. Perfurações com diferentes instrumentos apresentaram comportamento semelhante. Perfurações com sondas permitiram que orifícios confeccionados com dimensões superiores ao menor diâmetro interno do parafuso apresentassem resistência semelhante a das perfurações com dimensões iguais ao menor diâmetro interno do implante, realizadas com sondas e brocas. CONCLUSÕES: Nos de 4,2 mm o diâmetro e o modo de preparação do orifício influenciam o torque e a resistência, nos de 5,2 mm apenas o torque de inserção. Não há correlação entre força de arrancamento e torque de inserção. Nível de evidência II, Estudos terapêuticos - Investigação dos Resultados do Tratamento.

Reorganization of CA3 area of the mouse hippocampus after pilocarpine induced temporal lobe epilepsy with special reference to the CA3-septum pathway

We showed that when CA3 pyramidal neurons in the caudal 80% of the dorsal hippocampus had almost disappeared completely, the efferent pathway of CA3 was rarely detectable. We used the mouse pilocarpine model of temporal lobe epilepsy (TLE), and injected iontophoretically the anterograde tracer phaseolus vulgaris leucoagglutinin (PHA-L) into gliotic CA3, medial septum and the nucleus of diagonal band of Broca, median raphe, and lateral supramammillary nuclei, or the retrograde tracer cholera toxin B subunit (CTB) into gliotic CA3 area of hippocampus. In the afferent pathway, the number of neurons projecting to CA3 from medial septum and the nucleus of diagonal band of Broca, median raphe, and lateral supramammillary nuclei increased significantly. In the hippocampus, where CA3 pyramidal neurons were partially lost, calbindin, calretinin, parvalbumin immunopositive back-projection neurons from CA1-CA3 area were observed. Sprouting of Schaffer collaterals with increased number of large boutons in both sides of CA1 area, particularly in the stratum pyramidale, was found. When CA3 pyramidal neurons in caudal 80% of the dorsal hippocampus have almost disappeared completely, surviving CA3 neurons in the rostral 20% of the dorsal hippocampus may play an important role in transmitting hyperactivity of granule cells to surviving CA1 neurons or to dorsal part of the lateral septum. We concluded that reorganization of CA3 area with its downstream or upstream nuclei may be involved in the occurrence of epilepsy.