Brain Pathways Involved in the Modulatory Effects of Noradrenaline in Lateral Septal Area on Cardiovascular Responses


Autoria(s): Scopinho, America A.; Aguiar, Daniele C.; Resstel, Leonardo B. M.; Guimarães, Francisco S.; Correa, Fernando M. A.
Contribuinte(s)

UNIVERSIDADE DE SÃO PAULO

Data(s)

06/11/2013

06/11/2013

2012

Resumo

We have previously reported that stimulation of alpha-1 adrenoceptors by noradrenaline (NA) injected into the lateral septal area (LSA) of anaesthetized rats causes pressor and bradycardic responses that are mediated by acute vasopressin release into the circulation through activation of the paraventricular nucleus (PVN). Although the PVN is the final structure of this pathway, the LSA has no direct connections with the PVN, suggesting that other structures may connect these areas. To address this issue, the present study employed c-Fos immunohistochemistry to investigate changes caused by NA microinjection into the LSA in neuronal activation in brain structures related to systemic vasopressin release. NA microinjected in the LSA caused pressor and bradycardic responses, which were blocked by intraseptal administration of alpha-1 adrenoceptor antagonist (WB4101, 10 nmol/200 nL) or systemic V-1 receptor antagonist (dTyr(CH2)5(Me)AVP, 50 mu g/kg). NA also increased c-Fos immunoreactivity in the prelimbic cortex (PL), infralimbic cortex (IL), dorsomedial periaqueductal gray (dmPAG), bed nucleus of the stria terminalis (BNST), PVN, and medial amygdala (MeA). No differences in the diagonal band of Broca, cingulate cortex, and dorsolateral periaqueductal gray (dlPAG) were found. Systemic administration of the vasopressin receptor antagonist dTyr AVP (CH2)5(Me) did not change the increase in c-Fos expression induced by intra-septal NA. This latter effect, however, was prevented by local injection of the alpha-1 adrenoceptor antagonist WB4101. These results suggest that areas such as the PL, IL, dmPAG, BNST, MeA, and PVN could be part of a circuit responsible for vasopressin release after activation of alpha-1 adrenoceptors in the LSA.

CAPES [PNPD0176087]

CNPq

FAPESP

Identificador

CELLULAR AND MOLECULAR NEUROBIOLOGY, NEW YORK, v. 32, n. 7, pp. 1147-1157, OCT, 2012

0272-4340

http://www.producao.usp.br/handle/BDPI/42005

10.1007/s10571-012-9840-4

http://dx.doi.org/10.1007/s10571-012-9840-4

Idioma(s)

eng

Publicador

SPRINGER/PLENUM PUBLISHERS

NEW YORK

Relação

CELLULAR AND MOLECULAR NEUROBIOLOGY

Direitos

closedAccess

Copyright SPRINGER/PLENUM PUBLISHERS

Palavras-Chave #LATERAL SEPTUM #CARDIOVASCULAR REGULATION #C-FOS #VASOPRESSIN #RAT #MEDIAL PREFRONTAL CORTEX #METHYL-D-ASPARTATE #HYPOTHALAMIC PARAVENTRICULAR NUCLEUS #ACUTE IMMOBILIZATION STRESS #PERIAQUEDUCTAL GRAY AREA #ACUTE RESTRAINT STRESS #UNANESTHETIZED RATS #STRIA TERMINALIS #BED NUCLEUS #PRESSOR-RESPONSE #CELL BIOLOGY #NEUROSCIENCES
Tipo

article

original article

publishedVersion