Partial purification, immobilization and preliminary biochemical characterization of lipases from Rhizomucor pusillus

Lipases have important applications in biotechnological processes, motivating us to produce, purify, immobilize and perform a biochemical characterization of the lipase from Rhizomucor pusillus. The fungus was cultivated by solid state fermentation producing lipolytic activity of about 0.5 U/mL(4U/g). A partial purification by gel filtration chromatography in Se-phacryl S-100 allowed obtaining a yield of about 85% and a purification factor of 5.7. Our results revealed that the purified enzyme is very stable with some significant differences in its properties when compared to crude extract. The crude enzyme extract has an optimum pH and temperature of 7.5 ° C and 40 ° C, respectively. After purification, a shift of the optimum pH from 7 to 8 was observed, as well as a rise in optimumtemperature to 60 ° C and an increase in stability. The enzyme was immobilized on CNBr-Agarose and Octyl-Agarose supports, having the highest immobilization yield of 94% in the second resin. The major advantage of immobilization in hydrophobic media such as Octyl is in its hyper activation, which in this case was over 200%, a very interesting finding. Another advantage of this type of immobilization is the possibility of using the derivatives in biotechnological applications, such as in oil enriched with omega-3 as the results obtained in this study display the hydrolysis of 40% EPA and 7% DHA from sardine oil, promising results compared to the literature.

Educação matemática em escolas inclusivas: a sala de recursos em destaque

Pós-graduação em Educação Matemática - IGCE

Jogos de empresas e gestão financeira: algumas evidências

This article aims to discuss the use of business games for educational purposes taking into account evidence about the difficulty in managing financial resources. The use of business games is justified because the young generation tha are coming to the education system have grown up using business games, allowing a more motivating learning environment and stimulating investment and on how the concept to provide learning. Instead of pushing content well defined and wel-formatted for the student, promotes the use of games promote at the same time, a chalenge whose solution entails learning effort. Thus, the use of games affect educators, students and aducational institutions, requiring them new attitudes and patterns of thought about schooling. The elaboration of this article was based on experiences gained with the development and use of games for educational purposes, specifically the game "Mercado Virtual". Several articles have been published on research with indergraduates and postgraduate courses in business administration and engineering field. They have shown that the evaluation of the player, based on their decisions during the game, to detect specific aspects of learning. This exploratory research is based on data obteained in various experiments with the game Mercado Virtual.

Conflito, resistência e philía no Héracles furioso de Eurípides

In Heracles Mainomenos, by Euripides, the hero is submited to the ultimate test: win himself, accepting stay alive after committing an irremediable error. The dramatic action has dual motivation, human and divine. The human conflict requires resilience of families and friends of Heracles and the opposition of Lico is a key element to the action. In the subsequent clash between Heracles and the goddess Hera, victory lies with the deity, motivating the disaster, but confirms the heroics of Heracles, that resists the urge to annihilation after the murder of children. His friend, King Theseus, provides him the support needed to dissuade him from his purpose of annihilation and strengthens him for endurance. The valuation of philia is an important element in shaping the sense of this euripidean text.

Blog para o basquetebol: uma ferramenta no processo ensino-aprendizagem-treinamento dos sistemas táticos

Pós-graduação em Desenvolvimento Humano e Tecnologias - IBRC

Formação profissional e mediação pedagógica na era digital: educar para os meios e os meios para educar

Pós-graduação em Televisão Digital: Informação e Conhecimento - FAAC

Os efeitos da matriz bioparental nos processos de adoção de crianças e adolescentes

This paper is the result of a homonymous scientific research, funded by CNPq-PIBIC where we understand the adoption process as a process of dissidence in relation to the bio-parental matrix. Founded on a heteronormative naturalization of human sexuality - which presupposes a continuum and naturalized organization among sex / gender / desire – this bioparental matrix sets the binary relation of distinction between the legitimate/illegitimate child as their origin or not arising from “blood ties”. Considering our experience in the Project developed at the Department of Clinical Psychology at UNESP, Assis, SP called “Ties of love: Adoption, Gender, Citizenship and Rights”, we prepared a content analysis - as proposed by Bardin (1977) -, of transcripts of psychological sessions that were made from 2005 to 2012 in the "Center for Research and Applied Psychology “Dra. Betti Katzenstein. Our general objective was to analyze the effects of the bioparental matrix and its impact on children/adolescents and their families as well as estimate the possibilities of escape to the subjection to this bioparental matrix. The results showed us several aspects that may be significant for understanding the discursive crossings related to the practice of adoption. It was observed that there is still a great ambivalence pervading this theme, revealing that there is a discrepancy between what we say and what we do in relation to practices of caring among the adopted children. On the one hand, it was noticed that relatives rationally seek to enhance the bonding of the “emotional ties”, but their practices and beliefs, are still supported in modes of subjectivation that prioritize the biological discourse. This fact reveals a strained and conflictive field that probably weaknesses those families seeking to prioritize the ties of affection. However, as can be seen in this study, it is comforting and motivating to realize the power of resistance of individuals to absolute truths that govern their ways of feeling, affiliating and/ or exert their parenting.

Aprendendo isometria com mosaicos

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Redes sociais como ferramentas pedagógicas no ensino de biologia

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

A compreensão de alunos do ensino médio sobre as relações CTSA e a preservação de um ecossistema terrestre a partir de uma aprendizagem centrada em eventos

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

La Casa Nel Vicolo: um romance de Maria Messina à moda do “feuilleton”

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Especificidades da docência na infância: o foco no trabalho com bebês

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Sobre o uso de sistemas de computação algébrica na solução de problemas de mecânica das estruturas

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Estrutura informacional e clivagem: análise da veiculação de informação em construções clivadas do português brasileiro contemporâneo escrito

Pós-graduação em Linguística e Língua Portuguesa - FCLAR

Resolução de problemas com aplicações em funções

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)