Tartarugas marinhas da Bacia Potiguar/RN: diagnóstico, biologia alimentar e ameaças

This work aimed to study the diversity and distribution of marine sea turtles stranded in Potiguar Basin, Rio Grande do Norte, as well as aspects related to feeding behavior associated with human impacts. The study was conducted through the analysis of data from stranded animals, recorded in a daily monitoring in an area bounded on the north by the municipality of Aquiraz, in the state of Ceará, and the east by the municipality of Caicara do Norte, in the state of Rio Grande do Norte. Stranded dead animals were necropsied and for the analysis of the diet of animals, esophagus, stomach and intestines were fixed in 10% formalin and after that, the stomach contents were sorted and stored in 70% alcohol. Representative fragments of these organs were removed for making histological slides, with a view to histological characterization of the digestive tract. 2.046 occurrences of turtles were recorded during the period from 01/01/2010 to 31/12/2012. The Chelonia mydas species showed the highest number of records and it was observed in 66.81 % (N = 1,367) of cases; followed by Eretmochelys imbricata with 4.45 % (N = 91) and by Lepidochelys olivacea with 1.22% (N = 25). The Caretta caretta and Dermochelys coriacea species were, respectively, 0.93 % (N = 19) and 0.05 % (N = 1) records of strandings. In 26.54 % of cases, it was not possible to identify the species. Regarding the spatial distribution, the stretch A was the one that had the highest number of strandings and a larger number of records were registered in the warm months of the year. The dietary analysis showed that C. mydas fed preferentially on algae; C. caretta had a diet with a predominance of the item "coral´s fragments" and E. imbricata species showed preference for an animal origin material. Related to this anthropic interaction, 57.14 % (n = 76) of animals that died at the rehabilitation s base, showed cause of death due to complications from ingesting debris. According to the data presented, the Potiguar Basin presents itself as an area with important diversity and distribution of marine sea turtle as well is characterized as a feeding and nidification area for these species

Avaliação de diferentes formas de preservação e desenvolvimento in situ de folículos ovarianos pré-antrais caprinos

This study evaluated the effect of indole-3-acetic acid (IAA) in the activation of goats preantral follicles (FOPA). Were used four pairs of ovaries of adult mixed breed goats. Each ovarian pair was divided into 23 fragments. One fragment was fixed for histology and other fragment was using to follicular isolation procedure. The remaining fragments were cultured in 1.0 ml of Minimum Essential Medium (MEM) or MEM supplemented with IAA at concentrations of 10, 40, 100, 500 or 1000 ng/mL. The in vitro culture was performed at 39°C in incubator with 5% CO2 for 1, 3 and 5 days. After in vitro culture was evaluated histological integrity and viability of FOPA. The addition of 100 ng/ml of IAA to MEM showed a significant increase in follicles transition in the third day of in vitro culture, characterizing follicular activation. Moreover, this concentration was obtained maintaining the histological integrity of PAF by the fifth day of in vitro culture. The viability test confirmed the results of histology. Thus, we conclude that IAA can promote the activation of goats FOPA

Ultramorphology and histochemistry of fat body cells from last Instar larval of the Pachycondyla (=Neoponera) villosa (Fabricius) (Formicidae: Ponerinae)

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Structural and ultrastructural characteristics of interrenal gland and chromaffin cell of matrinxa, Brycon cephalus Gunther 1869 (Teleostei-Characidae)

This work presents the structure and ultrastructure of the interrenal gland and chromaffin cells, as well as the morphology of the head kidney of Brycon cephalus, the head kidney is composed of fused bilateral lobes located anterior to the swim bladder and ventrolateral to the spinal column, the parenchyma revealed lympho-haematopoietic tissue, melano-macrophage centres, interrenal gland and chromaffin cells. The interrenal gland consisted of cords or strands of cells grouped around the posterior cardinal vein and their branches. Chromaffin cells are found in small groups, closely associated with the interrenal gland and/or under the endothelium of the posterior cardinal vein. So far, the ultrastructural analysis has revealed only one interrenal cell type which contained abundant smooth endoplasmic reticulum and numerous mitochondria with tubulo-vesicular cristae, characteristic of steroid-producing cells. Two types of chromaffin cells were observed. The first type was characterized by the presence of vesicles with round, strongly electron-dense granules, which were eccentrically located, Such cells were interpreted as noradrenaline cells, Meanwhile, cells which contained smaller vesicles and electron-lucent granules, with a small halo separating the granule from the vesicular limiting membrane, were identified as adrenaline cells.

Abordagem inicial de lesões mamárias por biopsia helicóide: estudo experimental

OBJECTIVE: Evaluating the kit-Bh performance in carrying out of breast biopsies. METHODS: They were randomly selected a sample of 30 patients with breast cancer undergoing mastectomy, based on the results of a pilot study from February 2008 to April 2010. They were excluded women with had not palpable, stone-hard consistency tumors, previous surgical manipulation or that contains liquid. Using the helicoid biopsy Kit (kit Bh) and an equipment Core biopsy with cannula and needle and 14 gauge respectively, it was collected a fragment of sound equipment in the area and in tumors in each specimen, totaling 120 fragments for histological study. For data analysis, it was defined a 95% confidence level and used the SPSS-13 version, the Kappa index and the parametric Student t test. RESULTS: Mean age of patients was 51.6 years (± 11.1 years). The infiltrating ductal carcinoma showed a higher incidence, 26 cases (86.7%). The Core biopsy had a sensitivity of 93.3%, specificity of 100% and accuracy 96.7%, while the helicoid biopsy had a sensitivity of 96.7%, specificity of 100% and accuracy 98.3%. By comparing the histology of tumors and the fragments of biopsies, there was high degree of agreement in diagnoses (kappa of 0.93 with p <0.05) CONCLUSION: Both devices provided the histological diagnosis of lesions with high accuracy. Results of this study showed that the helicoid biopsy is a reliable alternative in 22 the preoperative diagnosis of breast lesions. Further studies in vivo better will define the role of Kit Bh in the diagnosis of these lesions

Sexagem histológica e desempenho de Oreochromis niloticus testando diâmetros de ração de acordo com o aparato bucal

Estudou-se o tamanho da boca de larvas de tilápia-do-nilo e testou-se o efeito de diferentes granulometrias da ração sobre o ganho de peso, comprimento e sobrevivência das larvas aos 30 e 60 dias de arraçoamento. Avaliou-se também o método de sexagem por meio de microscopia de luz aos 35 dias de idade. A medida da boca das larvas apresentou valores médios de 918,2±152,9μm aos cinco dias de idade. de acordo com esse dado, testaram-se três granulometrias: 0,25, 0,35 e 0,50mm. Aos 30 e aos 60 dias de arraçoamento, 10% das larvas foram medidas, pesadas e contadas para cálculo da taxa de sobrevivência. O tamanho dos grânulos testados não afetou o desempenho das larvas de tilápia nilótica com alimentação iniciada aos cinco dias pós-eclosão. Quanto à sexagem histológica aos 35 dias de idade, as gônadas apresentaram-se, em sua maioria, indiferenciadas. Recomenda-se que essa análise deva ser realizada de acordo com o tamanho dos animais e não com a idade.

Crescimento e estruturas do sistema digestório de larvas de pacu alimentadas com dieta microencapsulada produzida experimentalmente

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Efetividade de métodos de identificação sexual em tilápias-do-nilo (oreochromis niloticus) revertidas sexualmente com hormônio em ração com diferentes granulometrias

Three methods of sexual identification were evaluated in Nile tilapia (Oreochromis niloticus) at ages of 30, 60 and 90 days that were submitted to sexual reversion under administration of androgen hormone 17 alpha-methyltestosterone added to crumble diets with different granules sizes (0.25, 0.35 and 0.50 mm) in dosage of 60mg/kg. The techniques of sexual identification employed were: a) Macroscopic examination of urogenital papilla; b) Microscopic examination of gonads through stain with carmine-acetate and c) Microscopic examination of gonads through histological routine. In the presence of three methods of sexual identification evaluated, histology of gonads has shown the most confident diagnosis. The grain diameters of rations did not interfere in morphological characteristics of gonads, neither in secondary sexual characters of fish.

Intraoperative topical administration of mitomycin C, in different concentrations, on the cicatrization of mioplasties of the dorsal rectus of rabbits

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Sex-related macrostructural organization of the deer's brachial plexus

We describe the morphological organization of the deer brachial plexus in order to supply data to veterinary neuroclinics and anaesthesiology. The deer (Mazama gouazoubira) brachial plexus is composed of four roots: three cervical (C6, C7 and C8) and one thoracic (T1). Within each sex group, no variations are observed between the left and the right brachial plexus, though sex-related differences are seen especially in its origin. The origin of axillary and radial nerves was: C6, C7, C8 and T1 in males and C8-T1 (radial nerve) and C7, C8 and T1 (axillary nerve) in females; musculocutaneous nerve was: C6-C7 (males) and C8-T1 (females); median and ulnar nerves was: C8-T1 (males) and T1 (females); long thoracic nerve was: C7 (males) and T1 (females); lateral thoracic nerve was: C6, C7, C8 and T1 (males) and T1 (females); thoracodorsal nerve was: C6, C7, C8 and T1 (males) and C8-T1 (females); suprascapular nerve was: C6-C7 (males) and C6 (females) and subscapular nerve was: C6-C7 (males) and C7 (females). This study suggests that in male deer the origin of the brachial plexus is more cranial than in females and the origin of the brachial plexus is slightly more complex in males, i.e. there is an additional number of roots (from one to three). This sexual dimorphism may be related to specific biomechanical functions of the thoracic limb and electrophysiological studies may be needed to shed light on this morphological feature.

Canine spinal cord neuron and axon myelin sheath morphometry

This inedited morphometric study has been developed from healthy canine spinal cord neuron cytoplasm and nucleus, and white matter axonal myelin sheath, from cervical, thoracic and lumbar regions. For the morphometric study, the parameters were area, perimeter, maximum and minimum diameters and roundness for neurons and myelin thickness for axon. For each parameter, 300 neurons were analysed. The results revealed that lumbar neurons had the highest mean values for the analysed parameters, indicating the presence of large neurons in this region, with large axons as a result of myelin thickness, which is proportional to axon calibre. We conclude that these morphometric results can contribute for the establishment of normal patterns, for canine spinal cord cervical, thoracic and lumbar segments.

Morfologia do útero de cutias nulíparas e não nulíparas

Descreveu-se a morfologia do útero de cutias nulíparas e não nulíparas por meio de ovariossalpingoisterectomia. A análise macroscópica do útero foi realizada in loco e na peça retirada. Segmentos das porções proximal, média e distal do órgão foram colhidas, fixadas e preparadas histologicamente, sendo as amostras analisadas à microscopia de luz, e realizada a histomorfometria das camadas uterinas. Topograficamente, o útero localiza-se na região sublombar, caudalmente aos rins, em continuação aos ovários e às tubas uterinas, estendendo-se até à entrada da pelve, onde se posiciona dorsalmente à bexiga. Caracteriza-se como do tipo duplo, embora culmine em apenas um óstio cervical externo. Microscopicamente, a mucosa uterina é formada por variações do epitélio, de cilíndrico a pseudoestratificado, que se apoia em tecido conjuntivo frouxo, onde se observam glândulas endometriais revestidas por epitélio cilíndrico, além da presença de vasos sanguíneos. A camada muscular subdivide-se em interna ou submucosa, média ou vascular e externa ou subserosa. A camada serosa é composta por tecido conjuntivo e mesotélio. Na histomorfometria, verificou-se que a espessura uterina total e a espessura da camada mucosa, em média, foram maiores nas fêmeas não nulíparas.

Effect of probiotic and prebiotic inclusion in weaned piglet diets on structure and ultra-structure of small intestine

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Estudo comparativo dos efeitos do extrato de Ginkgo biloba L. e Panax ginseng C.A. Meyer na reprodução de ratos machos e fêmeas Wistar

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)