Proteomic identification of putative microRNA394 target genes in Arabidopsis thaliana identifies MLP family members critical for normal development

Expression of the F-Box protein Leaf Curling Responsiveness (LCR) is regulated by microRNA, miR394, and alterations to this interplay in Arabidopsis thaliana produce defects in leaf polarity and shoot apical meristem (SAM) organisation. Although the miR394-LCR node has been documented in Arabidopsis, the identification of proteins targeted by LCR F-box itself has proven problematic. Here, a proteomic analysis of shoot apices from plants with altered LCR levels identified a member of the Major Latex Protein (MLP) family gene as a potential LCR F-box target. Bioinformatic and molecular analyses also suggested that other MLP family members are likely to be targets for this post-translational regulation. Direct interaction between LCR F-Box and MLP423 was validated. Additional MLP members had reduction in protein accumulation, in varying degrees, mediated by LCR F-Box. Transgenic Arabidopsis lines, in which MLP28 expression was reduced through an artificial miRNA technology, displayed severe developmental defects, including changes in leaf patterning and morphology, shoot apex defects, and eventual premature death. These phenotypic characteristics resemble those of Arabidopsis plants modified to over-express LCR. Taken together, the results demonstrate that MLPs are driven to degradation by LCR, and indicate that MLP gene family is target of miR394-LCR regulatory node, representing potential targets for directly post-translational regulation mediated by LCR F-Box. In addition, MLP28 family member is associated with the LCR regulation that is critical for normal Arabidopsis development.

Infrared spectroscopic study of the phase transitions in CsNO3, RbNO3 and NH4NO3

Phase transitions of CsNO3 (II-I), RbNO3 (IV-III-II) and NH4NO3 (V-IV-III-II-I) have been studied by i.r. spectroscopy. The study has provided useful information on the changes in the dispositions of the ions during the phase transitions.

Infrared and Nuclear Quadrupole Resonance Studies of the Phase Transitions of Para-dichlorobenzene

The α→γ→α→β transitions of para-dichlorobenzene have been studied by employing infrared and n.q.r. spectroscopy as well as differential scanning calorimetry. The γ phase is associated with considerably higher values of some of the intramolecular vibration frequencies. The α→γ transition shows athermal nucleation behaviour as in martensitic transitions. Intermolecular vibration bands around 46 and 85 cm–1 present in γ and α phases disappear in the β phase. The α→β transition seems to be associated with some orientational disorder.

An infrared spectroscopic study of the low-spin-high-spin transition in FexMn1-x(Phen)2(NCS)2: A composition-induced change in the order of the spin-state transition

Infrared spectroscopy provides a valuable tool to investigate the spin-state transition in Fe(II) complexes of the type Fe(Phen)2(NCS)2. With progressive substitution of Fe by Mn, the first-order transition changes over to a second-order transition, with a high residual population of the high-spin state even at very low temperatures

Drosophila “enhancer-trap” transposants: Gene expression in chemosensory and motor pathways and identification of mutants affected in smell and taste ability

We have isolated about a thousandDrosophila P-element transposants that allow thein situ detection of genomic enhancer elements by a histochemical assay for β-galactosidase activity. We summarize the β-galactosidase staining patterns of over 200 such transposants in the adult. Our aim was to identify genes that are likely to be involved in the chemosensory and motor pathways ofDrosophila. Based on β-galactosidase expression patterns in the tissues of our interest, we have chosen some strains for further analysis. Behavioral tests on a subset of the transposants have, in addition, identified several strains defective in their chemosensory responses.

Geochemistry of water and ground water in the Nhecolandia, Pantanal of Mato Grosso, Brazil: Variability and associated processes

A distinctive feature of the Nhecolandia, a sub-region of the Pantanal wetland in Brazil, is the presence of both saline and freshwater lakes. Saline lakes used to be attributed to a past and phase during the Pleistocene. However, recent studies have shown that saline and fresh water lakes are linked by a continuous water table, indicating that saline water could come from a contemporary concentration process. This concentration process could also be responsible for the large chemical variability of the waters observed in the area. A regional water sampling has been conducted in surface and sub-surface water and the water table, and the results of the geochemical and statistical analysis are presented. Based on sodium contents, the concentration shows a 1: 4443 ratio. All the samples belong to the same chemical family and evolve in a sodic alkaline manner. Calcite or magnesian calcite precipitates very early in the process of concentration, probably followed by the precipitation of magnesian silicates. The most concentrated solutions remain under-saturated with respect to the sodium carbonate salt, even if this equilibrium is likely reached around the saline lakes. Apparently, significant amounts of sulfate and chloride are lost simultaneously from the solutions, and this cannot be explained solely by evaporative concentration. This could be attributed to the sorption on reduced minerals in a green sub-surface horizon in the "cordilhieira" areas. In the saline lakes, low potassium, phosphate, magnesium, and sulfate are attributed to algal blooms. Under the influence of evaporation, the concentration of solutions and associated chemical precipitations are identified as the main factors responsible for the geochemical variability in this environment (about 92 % of the variance). Therefore, the saline lakes of Nhecolandia have to be managed as landscape units in equilibrium with the present water flows and not inherited from a past and phase. In order to elaborate hydrochemical tracers for a quantitative estimation of water flows, three points have to be investigated more precisely: (1) the quantification of magnesium involved in the Mg-calcite precipitation; (2) the identification of the precise stoichiometry of the Mg-silicate; and (3) the verification of the loss of chloride and sulfate by sorption onto labile iron minerals.

A Phase Transition for the Uniform Distribution in the Pattern Maximum Likelihood Problem

In this paper, we consider the setting of the pattern maximum likelihood (PML) problem studied by Orlitsky et al. We present a well-motivated heuristic algorithm for deciding the question of when the PML distribution of a given pattern is uniform. The algorithm is based on the concept of a ``uniform threshold''. This is a threshold at which the uniform distribution exhibits an interesting phase transition in the PML problem, going from being a local maximum to being a local minimum.

Estudios de Historia de España, 2005, vol. 7 (número completo)

Contenido: Problemas para la localización del texto base de un fragmento neotestamentario incluído en Almaqãmi’ Assulbãn de Alhazrağī (S. XII) / Juan Pedro Monferrer Sala – Pax alphonsina: el ideal de imperium y el conocimiento en algunos textos alfonsíes / Santiago Disalvo – El niño en al-Andalus a través de la medicina y el derecho / Camilo Álvarez de Morales – Algunos aspectos de la castellanización de Álava. El conflicto en torno a la sucesión del señorío de Ayala en 1332 / Juan de la Cruz Ojeda – As potencialidades da função de Aia na Baixa Idade Média / Fátima Regina Fernandes – Los que comercian con dinero. 2ª parte / María del Carmen Carlé – Ysabel y la excelente señora en 1504: ¿Razón de estado o usurpación a un trono? Un nuevo documento / István Szászdi León-Borja – La elite de los artesanos (siglo XV y XVI) / María Concepción Rodríguez de Monteagudo – Los milagros de Guadalupe como fuente histórica para la reconstrucción de la vida en la frontera (España, Siglos XV y XVI) / Gerardo Rodríguez – El pensamiento agónico de Unamuno referido a la idea de lo nacional / Evelyn Klein -- Reseñas

El Cancioneiro Geral portugués y algunas distinciones de su congénere, El Cancionero General

Integran este número de la revista ponencias presentadas en Studia Hispanica Medievalia VIII : Actas de las X Jornadas Internacionales de Literatura Española Medieval, 2011, y de Homenaje al Quinto Centenario del Cancionero General de Hernando del Castillo.

Efecto de tres laminas de riego y tres dosis de aplicacion de biofertilizante en el cultivo orgánico de fresa (Fragaria spp.) cv Festival en el Castillito, Las Sabanas, Madriz

El presente estudio se realizó con productores de fresa de la comunidad de El Castillito, municipio de Las Sabanas, Madriz, durante el período de abril 2009–abril 2010, con el propósito de conocer los efectos de normas de riego y dosis de biofertilizante en el cultivo de fresa (Fragaria spp.). Se utilizó un diseño de Bloques Completo al Azar (BCA), con un arreglo en franjas con tres réplicas. Se trabajó en parcelas de tres productores, Humberto, Cristian, Celso, las láminas de establecidas en tiempo de 15, 25 y 30 min por día, y las dosis de biofertilizante de 200, 400 y 600 l ha-1. Las variables fueron sujetas a un análisis de varianza (ANDEVA) y agrupaciones de medias mediante LSD (∞=0.05). Las variables de hojas y coronas no fueron afectadas significativamente por los factores. El volumen y peso de fruto fueron afectadas por las normas de riego; no así en la longitud, diámetro y grados Brix. Las dosis de biofertilizante de 600 l ha-1 mostraron efecto significativo en el peso del fruto. De igual manera, los factores evaluados afectaron de manera significativa los rendimientos obtenidos, con valores promedios de 1509.24 kg ha-1 y 1188.24 kg ha-1, para los niveles de 30 minutos y 600 l ha-1, respectivamente. El mayor rendimiento total fue de 3132 kg ha-1 en el tratamiento 30 minutos y 400 l ha-1 en el productor Humberto. Basados en la información mensual de las propiedades psicométricas del aire, se tomaron variables como temperatura, humedad relativa, velocidad del viento y precipitaciones. Se constató que El Castillito cuenta con un ambiente propicio para el desarrollo del cultivo de fresa.

Sapientia, 1963, Vol. XVIII, nº 69-70 (número completo)

Contenido: Materialismo dialéctico y totalitarismo / Octavio N. Derisi – Luis Lavelle / Regis Jolivet – La continuidad entre la dogmática jurídica, la teoría general y la filosofía del derecho / Avelino M. Quintas – Lógica y logística / Carlos A. Lungarzo Melcon – La ética existencialista y marxista / Octavio N. Derisi – Ontología del arte moderno / Ángel T. Lo Celso – Notas y comentarios -- Bibliografía

Ernani Sátyro

Apresenta dados e depoimentos relacionados à vida política do ex-Deputado Ernani Sátyro, representante do povo paraibano na Câmara dos Deputados durante oito mandatos, de 1946 a 1986, ano de sua morte. Os discursos reunidos, uma pequena parte do universo de seus pronunciamentos na tribuna do plenário e nas comissões, evidenciam sua constante preocupação com os problemas brasileiros, com as aflições do Nordeste e com os anseios paraibanos.

Sucessores bem sucedidos? Um balanço realista (e completo) da diplomacia na era militar

Apresenta uma resenha sobre o volume 2 da publicação - Os sucessores do Barão: relações exteriores do Brasil, 1912-1964 - que aborda a história das relações internacionais e da política externa do Brasil durante o regime militar, cobrindo tanto os episódios diplomáticos quanto os relativos ao quadro político e econômico mundial.

Ausência de política pública de cotas raciais para ingresso em instituições públicas de ensino superior no Brasil

Descreve o atual panorama normativo para as cotas raciais no Brasil com base em estudo realizado com utilização de a análise documental e bibliográfica. Os resultados indicaram que a ausência de uma norma federal implicou baixa adesão ao sistema de cotas, o que é ratificado pelo insignificante número de Instituições Públicas de Ensino Superior (Ipes) que adotaram norma de cota racial - apenas 17,79%. Verificou-se, ainda, que essa ausência cria lacunas na adoção de diretrizes nacionais para a interpretação e a compreensão das ações afirmativas. Tais lacunas refletem diretamente no ciclo da política pública, comprometendo a avaliação e o acompanhamento da efetividade e do sucesso da política, o que é extremamente perigoso para a segurança jurídica na área de direitos humanos e para a garantia da equidade de fato nos espaços político, econômico e social.