Burial depth and cultivation influence emergence and persistence of Phalaris paradoxa seed in an Australian sub-tropical environment

Emergence and persistence characteristics of Phalaris paradoxa seeds in no- and minimum-till situations and at different burial depths were studied in a sub-tropical environment. Three experiments were carried out using naturally shed seeds. In the first experiment, seedlings emerged from May through to September each year, although the majority of seedlings emerged in July. In the second experiment with greater seed density, cultivation in March of each year stimulated seedling emergence, altered the periodicity of emergence and accelerated the decline of seeds in the seedbank compared with plots that received no cultivation. The majority of seedlings in the cultivated plots emerged in May whereas the majority of seedlings in the undisturbed plots emerged in July. Emergence accounted for only 4-19% of the seedbank in both experiments over 2 years. Seed persistence was short in both field experiments, with less than 1% remaining 2 years after seed shed. In the third experiment, burial depth and soil disturbance significantly influenced seedling emergence and persistence of seed. Seedlings emerged most from seed mixed in the top 10 cm when subjected to annual soil disturbance, and from seed buried at 2.5 and 5.0 cm depths in undisturbed soil. Emergence was least from seed on the soil surface, and buried at 10 and 15 cm depths in undisturbed soil. Seeds persisted longest when shed onto the soil surface and persisted least when the soil was tilled. These results suggest that strategic cultivation may be a useful management tool, as it will alter the periodicity of emergence allowing use of more effective control options and will deplete the soil seedbank more rapidly.

Lasers - An effective artificial source of radiation for the cultivation of anoxygenic photosynthetic bacteria

The laser diode (LD) is a unique light source that can efficiently produce all radiant energy within the narrow wavelength range used most effectively by a photosynthetic microorganism. We have investigated the use of a single type of LID for the cultivation of the well-studied anoxygenic photosynthetic bacterium, Rhodobacter capsulatus (Rb. capsulatus). An array of vertical-cavity surface-emitting lasers (VCSELs) was driven with a current of 25 mA, and delivered radiation at 860 nm with 0.4 nm linewidth. The emitted light was found to be a suitable source of radiant energy for the cultivation of Rb. capsulatus. The dependence of growth rate on incident irradiance was quantified. Despite the unusual nearly monochromatic light source used in these experiments, no significant changes in the pigment composition and in the distribution of bacteriochlorophyll between LHII and LHI-RC were detected in bacterial cells transferred from incandescent light to laser light. We were also able to show that to achieve a given growth rate in a light-limited culture, the VCSEL required only 30% of the electricity needed by an incandescent bulb, which is of great significance for the potential use of laser-devices in biotechnological applications and photobioreactor construction. (c) 2006 Wiley Periodicals, Inc.

Effect of cultivation on soil C contents and saturated hydraulic conductivity

The irrigation of pasture with saline, Na-contaminated industrial wastewater typically results in an increase in soil ESP. From current knowledge (derived largely from cultivated agricultural soils), although these sodic soils are likely to remain stable whilst irrigated with effluent (due to the effluent’s large electrolyte concentration), during rainfall periods of low electrolyte concentration these soils would be expected to disperse. However, effluent irrigated pasture soils have been observed to maintain their structure even during intense rainfall events. Three soil types were collected (Sodosol, Vertosol and Dermosol), each with a cultivated/non-cultivated pair. The soils were equilibrated with various SAR solutions and then leached with deionised water to allow the measurement of saturated hydraulic conductivity (Ksat). At low SARs, Ksat tended to be greater in non-cultivated than cultivated soils and is attributable to a loss of structure associated with cultivation. In addition, as SAR increased, the reduction in relative Ksat tended to be significantly greater in cultivated than non-cultivated soils. The relatively rapid saturated hydraulic conductivity in the non-cultivated soils at large SARs is due to a greater aggregate stability due to greater soil C content. For the sustainable disposal of saline effluent, it is therefore necessary to ensure that soils remain undisturbed and preferably under pasture, thus maximising soil structural stability and hydraulic conductivity.

Parameter Identification of a Fed-Batch Cultivation of S. Cerevisiae using Genetic Algorithms

Fermentation processes as objects of modelling and high-quality control are characterized with interdependence and time-varying of process variables that lead to non-linear models with a very complex structure. This is why the conventional optimization methods cannot lead to a satisfied solution. As an alternative, genetic algorithms, like the stochastic global optimization method, can be applied to overcome these limitations. The application of genetic algorithms is a precondition for robustness and reaching of a global minimum that makes them eligible and more workable for parameter identification of fermentation models. Different types of genetic algorithms, namely simple, modified and multi-population ones, have been applied and compared for estimation of nonlinear dynamic model parameters of fed-batch cultivation of S. cerevisiae.

Molecular diversity, cultivation, and improved detection by fluorescent in situ hybridization of a dominant group of human gut bacteria related to Roseburia spp. or Eubacterium rectale

Peer reviewed

Molecular diversity, cultivation, and improved detection by fluorescent in situ hybridization of a dominant group of human gut bacteria related to Roseburia spp. or Eubacterium rectale

Peer reviewed

Education and Self-Cultivation: Foundational Changes for Existential Flourishing

The strained relationship between pedagogy and self-cultivation is often overlooked in favour of more pressing and profitable concerns within educational institutions. However, the end of education is not a well-paying job. It is often thought that education will produce a certain type of learned individual. Students are entered into educational institutions from a very young age and spend most of their youth within them, subsequently forming a part of themselves, their self-identify and agency, through these institutions. A significant amount of trust is placed in the hands of educators not only to impart information about various subject matters but to teach students how to think. This, however, is a difficult task which cannot be completely accomplished; thinking is a skill which an educator can promote but only the individual can cultivate. If self-cultivation is not the end of pedagogical practice and education, there is something deeply contradictory between the theoretical and practical values and aims of educational institutions.This thesis will address the problems caused by the neglect of self-cultivation within the pedagogical practices of contemporary educational institutions. I argue that self-cultivation, existential learning and flourishing should be the focus of these institutions. Educational institutions can make alternative efforts not only to improve the learning environment for these students but to prepare them to cope with existential questions. By acknowledging and focusing on the significance of self-cultivation, educational institutions can and should make efforts not only to teach useful, marketable skills and information but to also nurture the agency and mental well-being of the student him- or herself. My hope is that this thesis will be the first step towards answering a larger concern: How can educational institutions alleviate the struggles of students, particularly the seemingly growing number who are suffering from depression, by appealing to notions of self-cultivation?

The expansion of soybean cultivation in Campeche, México: Problematic and perspectives

The soybean is the grain in which greater food dependency has Mexico, reason why as of 2008, the government has promoted his culture, granting excellent subsidies, as much to producers as to buyers of the grain, thus contributing to a recent process of expansion in certain states, as it happens in Campeche. The objetive of this article is the analysis of the characteristics and effects of those supports, as well as of the rest of factors that until today they have taken to the producers of the mentioned state to initiate or to expand the cultivation of the soybean. The findings of the investigation reveal that although the producers have improved their levels of income, the process is vulnerable, as it depends on variables like the governmental supports, the international prices of the soybean and exchange rate. Although the study of the negative effects of genetically modified soybeans (GM) in other areas (environment, biodiversity, deforestation, human and animal health) is not the purpose of this investigation, some information will be provided, as on the conflict between soybean producers and beekeepers in the state of Campeche.

Hydrological and economic effects of oil palm cultivation in Indonesian peatlands

Oil palm has increasingly been established on peatlands throughout Indonesia. One of the concerns is that the drainage required for cultivating oil palm in peatlands leads to soil subsidence, potentially increasing future flood risks. This study analyzes the hydrological and economic effects of oil palm production in a peat landscape in Central Kalimantan. We examine two land use scenarios, one involving conversion of the complete landscape including a large peat area to oil palm plantations, and another involving mixed land use including oil palm plantations, jelutung (jungle rubber; (Dyera spp.) plantations, and natural forest. The hydrological effect was analyzed through flood risk modeling using a high-resolution digital elevation model. For the economic analysis, we analyzed four ecosystem services: oil palm production, jelutung production, carbon sequestration, and orangutan habitat. This study shows that after 100 years, in the oil palm scenario, about 67% of peat in the study area will be subject to regular flooding. The flood-prone area will be unsuitable for oil palm and other crops requiring drained soils. The oil palm scenario is the most profitable only in the short term and when the externalities of oil palm production, i.e., the costs of CO2 emissions, are not considered. In the examined scenarios, the social costs of carbon emissions exceed the private benefits from oil palm plantations in peat. Depending upon the local hydrology, income from jelutung, which can sustainably be grown in undrained conditions and does not lead to soil subsidence, outweighs that from oil palm after several decades. These findings illustrate the trade-offs faced at present in Indonesian peatland management and point to economic advantages of an approach that involves expansion of oil palm on mineral lands while conserving natural peat forests and using degraded peat for crops that do not require drainage.

Jatropha cultivation in Malawi and Mozambique: impact on ecosystem services, local human well-being, and poverty alleviation

Jatropha-based biofuels have undergone a rapid boom-and-bust cycle in southern Africa. Despite strong initial support by governments, donors, and the private sector, there is a lack of empirical studies that compare the environmental and socioeconomic impacts of Jatropha’s two dominant modes of production: large plantations and smallholder-based projects. We apply a rapid ecosystem services assessment approach to understand the impact of two Jatropha projects that are still operational despite widespread project collapse across southern Africa: a smallholder-based project (BERL, Malawi) and a large plantation (Niqel, Mozambique). Our study focuses on changes in provisioning ecosystem services such as biofuel feedstock, food, and woodland products that can have important effects on human well-being locally. Qualitative information is provided for other regulating and cultural ecosystem services. Although at this stage no impact is tremendously positive or negative, both projects show some signs of viability and local poverty alleviation potential. However, their long-term sustainability is not guaranteed given low yields, uncertain markets, and some prevailing management practices.

Optimizing immobilized cultivation of Haematococcus pluvialis for astaxanthin production

Die Produktion von Astaxanthin mittels Haematococcus pluvialis ist eines der wichtigsten Fachgebiete der Mikroalgen Biotechnologie. Kommerzielles Interesse ist zurückzuführen auf dessen Anwendungen als Pigment in der Tierzucht und neuerdings auch im Bereich des Gesundheitswesens, begründet auf seiner starken anti-oxidativen Wirkung. Allerdings begrenzen die Herausforderungen in der industriellen Produktion von H. pluvialis, in Bezug auf den Metabolismus der Alge wie gleichermaßen auch die Kultivierungsstrategie, immer noch den Markt für natürliches Astaxanthin. Die derzeit gängigen Kultivationstechnologien basieren auf Suspensionssystemen, welche weitgehende Nachteile mit sich führen, wie hoher Wasser-, Energie und Technikaufwand. Diese Probleme können durch die Anwendung einer immobilisierten Kultivation vermieden werden, weshalb diesem Verfahren immer mehr Aufmerksamkeit zu Teil wird. In dieser Arbeit wurde H. pluvialis als immobilisierte Kultur in einem Twin-Layer PSBR verwendet. Insgesamt wurden 26 verschiedene Arten von H. pluvialis gefunden, die als Biofilm wachsen und Astaxanthin produzieren konnten, wobei der Stamm CCAC 0125 zur weiteren Optimierung des Prozesses ausgewählt wurde. Biomasse und Astaxanthin Produktion stiegen zunehmenden in Abhängigkeit zu der Lichtintensität, bis einschließlich 1,015 µmol Photonen m-2 s-1, ohne Anzeichen von Photoinhibition. Maximale Biomasse Produktivität von 19.4 g m-2 d-1 wurde unter starken Lichtverhältnissen verzeichnet. Nährstofflimitierungen und Salinität unterstützen die Astaxanthin Produktion hinderten allerdings das Wachstum der Biomasse. Nichtdestotrotz, wurde die höchste Astaxanthin Produktivität von 0.507 g m-2 d-1 mit einem Biomasseanteil von 3.5% in der Trockenmasse durch starke Belichtung sowie Stickstoff- und Phosphatmangel erreicht. Diese Ergebnisse bestärken die Verwendung der immobilisierten Kultivation von H. pluvialis als Alternative zu den derzeitigen Technologien. Die Verknüpfung von hoher Biomasse mit der Produktion von Astaxanthin bei starkem Lichtintensitäten wurde nur durch die Anordnung in einem Biofilm ermöglicht. Es kann einen Durchbruch in der kommerziellen Herstellung von H. pluvialis darstellen, da hierbei das Vorziehen der Kulturen bei geringen Licht entfällt, was wiederum den derzeit verwendeten komplexen Zwei-Stufen Prozess stark vereinfacht.

The book of choice ferns for the garden, conservatory, and stove. Describing and giving explicit cultural directions for the best and most striking ferns and selaginellas in cultivation. Illustrated with coloured plates and numerous wood engravings, specially prepared for this work.

vol.I. Introduction to Athyrium.--vol.II. Blechnum to Nothochlaena.--vol.III. Ochropteris to Woodwardia, and Selaginella.

Firepolished glass microtools for human embryonic stem cell (HESC) cultivation

Abstract not available