The downy mildew resistance locus Pp523 is located on chromosome C8 of Brassica oleracea L.

We have previously constructed a genetic map of Brassica oleracea L. containing the Pp523 locus that confers downy mildew resistance to adult plants. In this work, 44 SSR markers of reference for the Brassica C genome chromosomes were added to the map, allowing the nine major linkage groups to be assigned to the nine chromosomes of B. oleracea. Locus Pp523 was located on chromosome C8, and a locus determining flower colour was mapped to chromosome C3. In comparison with the first version of the map, the new map is denser and more compact. The available genomic information on B. oleracea was enriched with the chromosome location of two phenotypic traits and 421 DNA markers (RAPD, ISSR, AFLP, SCAR, BAC-end derived STS, SSR and other PCR markers). Conversely, the genomic information on B. oleracea chromosome C8 is being used as an additional tool for the map-based cloning of Pp523, the first gene for adult plant resistance to downy mildew precisely located to a specific chromosome of this crop species.

Volatility and VaR Forecasting in the Madrid Stock Exchange

This paper provides an empirical study to assess the forecasting performance of a wide range of models for predicting volatility and VaR in the Madrid Stock Exchange. The models performance was measured by using different loss functions and criteria. The results show that FIAPARCH processes capture and forecast more accurately the dynamics of IBEX-35 returns volatility. It is also observed that assuming a heavy-tailed distribution does not improve models ability for predicting volatility. However, when the aim is forecasting VaR, we find evidence of that the Student’s t FIAPARCH outperforms the models it nests the lower the target quantile.

Fiscal policy under financial stress: A threshold VAR approach

The purpose of the project is to measure the impact of fiscal policy on the Portuguese GDP and how it may vary according to the state of the financial market. A Threshold VAR model is presented in which the two regimes are found using a financial stress index that divides the economy into a situation of financial stress and financial stability.

Var går gränsen? : vårdnadshavares uppfattning om lärares ansvarsområden.

Syftet med denna avhandling är att undersöka vårdnadshavares uppfattning om lärares ansvarsområden utöver undervisning. Ansvarsområdena som undersökts har namngetts tillgänglighet, fostran och lärares externa uppgifter. Med tillgänglighet avses kontakten mellan hem och skola, med fostran avses hemmets respektive skolans ansvar för fostran och med lärares externa uppgifter avses uppgifter som lärare har trots att uppgifterna enligt lag inte är en del av läraruppdraget. Skillnader i vårdnadshavares uppfattning har analyserats utgående från vårdnadshavares utbildningsgrad och familjesituation. Utgående från avhandlingens syfte har följande forskningsfrågor utformats: 1. Vad är vårdnadshavares uppfattning om ansvarområdena tillgänglighet, fostran och lärares externa uppgifter? 2. Hurdana skillnader finns det i vårdnadshavares uppfattning om lärares ansvarsområden utgående från vårdnadshavares utbildningsgrad? 3. Hurdana skillnader finns det i vårdnadshavares uppfattning om lärares ansvarsområden utgående från vårdnadshavares familjesituation? För att besvara ovanstående forskningsfrågor användes webbenkät som datainsamlingsmetod, vilket innebär att undersökningen är kvantitativ. Undersökningen har även kvalitativa inslag på grund av ett fåtal öppna frågor i webbenkäten. Forskningsansatsen är positivistisk. För att analysera materialet gjordes envägs variansanalys (one-way ANOVA) och engrupps t-test i statistikprogrammet SPSS. Respondenterna för undersökningen är vårdnadshavare till elever i årskurs tre och fyra i svenskspråkiga skolor från tre städer i Svenskfinland. Det totala antalet respondenter i undersökningen är 79. Resultatet visar att vårdnadshavarna uppfattar ansvarsområdet tillgänglighet vara mindre viktigt än det neutrala mittenvärdet samt att ansvaret för fostran och externa uppgifter inte enbart är lärarens. Vid analys av ansvarsområdena utgående från utbildningsgrad visar endast resultatet för analysen av ansvarsområdet tillgänglighet på signifikanta skillnader. Skillnaden kan ses mellan vårdnadshavarna med examen på andra stadiet respektive universitetsexamen. Resultatet visar dock att vårdnadshavarna med examen på andra stadiet anser att lärare har större ansvar för både fostran och externa uppgifter jämfört med vad vårdnadshavarna med yrkeshögskoleexamen respektive universitetsexamen gjorde. Vid analys av ansvarsområdena utgående från familjesituation visar resultatet inga signifikanta skillnader. Resultaten visar dock att vårdnadshavarna med äldre barn som går eller har gått i grundskolan anser att lärare har större ansvar för fostran jämfört med vad vårdnadshavarna vars barn är det första i grundskolan anser. Vid motsvarande analys av externa uppgifter anser vårdnadshavarna vars barn är det första i grundskolan att lärare har större ansvar.

1.° Petri Pauli Vergerii, Justinopolitani, liber de politia Venetorum, eorumque legibus ac moribus, urbis situ, naturaque regionis. — 2.° Ejusdem liber de situ veteris Romae. — 3.° Anonymi chronica Italica. — 4.° Historiae ecclesiasticae omnium temporum libri sex : authore anonymo. — 5.° Sexti Rufi, viri Consularis, historiae Romanae breviarium. — 6.° Benevenuti de Rambaldis, Imolensis, liber augustalis de vitis Caesarum.

Faurianus

The determination of sinigrin in Brassica, and investigations into the use of allyl-isothiocyanate as a nematicide

The goal of this thesis was to study factors related to the development of Brassica juncea as a sustainable nematicide. Brassica juncea is characterized by the glycoside (glucosinolate) sinigrin. Various methods were developed for the determination of sinigrin in Brassica juncea tissue extracts. Sinigrin concentrations in plant tissues at various stages of growth were monitored. Sinigrin enzymatically breaks down into allylisothiocyanate (AITC). AITC is unstable in aqueous solution and degradation was studied in water and in soil. Finally, the toxicity of AITC against the root-lesion nematode (Pratylenchus penetrans) was determined. A method was developed to extract sinigrin from whole Brassica j uncea tissues. The optimal time of extraction wi th boiling phosphate buffer (0.7mM, pH=6.38) and methanol/water (70:30 v/v) solutions were both 25 minutes. Methanol/water extracted 13% greater amount of sinigrin than phosphate buffer solution. Degradation of sinigrin in boiling phosphate buffer solution (0.13%/minute) was similar to the loss of sinigrin during the extraction procedure. The loss of sinigrin from boiling methanol/water was estimated to be O.Ol%/minute. Brassica juncea extract clean up was accomplished by an ion-pair solid phase extraction (SPE) method. The recovery of sinigrin was 92.6% and coextractive impurities were not detected in the cleaned up extract. Several high performance liquid chromatography (HPLC) methods were developed for the determination of sinigrin. All the developed methods employed an isocratic mobile phase system wi th a low concentration of phosphate buffer solution, ammonium acetate solution or an ion-pair reagent solution. A step gradient system was also developed. The method involved preconditioning the analytical column with phosphate buffer solution and then switching the mobile phase to 100% water after sample injection.Sinigrin and benzyl-glucosinolate were both studied by HPLC particle beam negative chemical ionization mass spectrometry (HPLCPB- NCI-MS). Comparison of the mass spectra revealed the presence of fragments arising from the ~hioglucose moiety and glucosinolate side-chain. Variation in the slnlgrin concentration within Brassica juncea plants was studied (Domo and Cutlass cuItivars). The sinigrin concentration in the top three leaves was studied during growth of each cultivar. For Cutlass, the minimum (200~100~g/g) and maximum (1300~200~g/g) concentrations were observed at the third and seventh week after planting, respectively. For Domo, the minimum (190~70~g/g) and maximum (1100~400~g/g) concentrations were observed at the fourth and eighth week after planting, respectively. The highest sinigrin concentration was observed in flower tissues 2050±90~g/g and 2300±100~g/g for Cutlass and Domo cultivars, respectively. Physical properties of AITC were studied. The solubility of AITC in water was determined to be approximately 1290~g/ml at 24°C. An HPLC method was developed for the separation of degradation compounds from aqueous AITC sample solutions. Some of the degradation compounds identified have not been reported in the literature: allyl-thiourea, allyl-thiocyanate and diallyl-sulfide. In water, AITC degradation to' diallyl-thiourea was favored at basic pH (9.07) and degradation to diallyl-sulfide was favored at acidic pH (4 . 97). It wap necessary to amend the aqueous AITC sample solution with acetonitrile ?efore injection into the HPLC system. The acetonitrile amendment considerably improved AITC recovery and the reproducibility of the results. The half-life of aqueous AITC degradation at room temperature did not follow first-order kinetics. Beginning with a 1084~g/ml solution, the half-life was 633 hours. Wi th an ini tial AITC concentration of 335~g/ml the half-life was 865 hours. At 35°C the half-life AITC was 76+4 hours essentially independent of the iiisolution pH over the range of pH=4.97 to 9.07 (1000~g/ml). AITC degradation was also studied in soil at 35°C; after 24 hours approximately 75% of the initial AITC addition was unrecoverable by water extraction. The ECso of aqueous AITC against the root-lesion nematode (Pratylenchus penetrans) was determined to be approximately 20~g/ml at one hour exposure of the nematode to the test solution. The toxicological study was also performed with a myrosinase treated Brassica juncea extract. Myrosinase treatment of the Brassica juncea extract gave nearly quantitative conversion of sinigrin into AITC. The myrosinase treated extract was of the same efficacy as an aqueous AITC solution of equivalent concentration. The work of this thesis was focused upon understanding parameters relevant to the development of Brassica juncea as a sustainable nematicide. The broad range of experiments were undertaken in support of a research priority at Agriculture and Agri-Food Canada.

Biochemical and histological investigations of viral localisation in the hypersensitive reaction of Phaselous vulgaris L. var Pinto to tobacco mosaic virus infection

STOBBS, Lorne,W ABSTRACT Biochemical and Histological Investigations of viral localisation in the hypersensitive reaction of Phaseolus vulgaris L. var Pinto to tobacco mosaic virus infection. The infection of Phaseolus vulgaris L. var Pinto with tobacco mosaic virus (TMV) results in the production of distinct necrotic lesions confining the virus to restricted areas of the leaf surface. Biochemical and histological changes in the leaf tissue as a result of infection have been described. Trace accumulations of fluorescent metabolites, detected prior to lesion expression represent metabolites produced, by the cell in response to virus infection. These substances, are considered to undergo oxidation and in diffusing into adjacent cells, react with cellular constituents causing the death of these cells. Such cellular necrosis in advance of infection effectively limits virus spread. Chromatographic studies on extracts from TMV infected Pinto bean leaf tissue suggests that a number of extra-fluorescent metabolites produced on lesion'expression represent end products of phenolic oxidation r,eactionsoccurring earlier in these cells. Inhibition of phenolic oxidation by ascorbate infiltration or elevated temperature treatment resulted in the absence of extra-fluorescent metabolites and the continued movement of virus in the absence of necrosis. Further studies with i ascorbate infiltration indicated that irreversible necrotic events were determined as early as 12 tci 18 hrs after viral inoculation. Histochemical tests indicated that callose formation was initiated at this time, and occurred in response to necrotisation. Inhibition of necrosis by either ascorbate infiltration or elevated temp8rature treatment resulted in the absence of callose deposition. Scanning electron'micrographs of infected tissue revealed severe epidermal and palisade cell damage. Histochemical tests indicated extensive callose formation in cells bordering the lesion, and suggested the role of callose iTh the blockage of intercellular connections limiting virus movement. The significance of these cellular changes is discussed. ii

Producción y composición quimica del forraje de Huizachillo (Desmanthus virgatus (L) var. de pressus Willd) bajo cultivo.

Tesis (Maestría en Ciencias en Producción Agrícola) UANL

Efecto de la tensión hídrica edáfica en diferentes etapas del desarrollo sobre el crecimiento de Phaseolus acutifolius var. latifolius F. y Phaseolus vulgaris L.

Tesis (Maestría en Ciencas Especialidad en Producción Agrícola) UANL

Purificación y caracterización de una proteína antitumoral a partir de Bacillus thuringiensis var. thuringiensis.

Tesis (Maestría en Ciencias con Especialidad en Inmunobiología) UANL

Efecto de tres dosis de polímero y tres dosis de fertilizante en la producción de plántula de chile serrano (Capsicum annuum L.) var. Tampiqueño 74

Tesis (Maestría en Ciencias en Producción Agrícola) UANL