p53蛋白质Gly249和Ser249替换型三维结构的分子动力学研究

p53蛋白质核心区249位点精氨酸被其他残基替换后能引起p53蛋白质核心区L2、L3结构域间的密切联系趋于松散,正常的空间构象发生改变并使整个核心区结构稳定性受到破坏。

Ovulation in the tree shrew (Tupaia belangeri) induced by gonadotrophins

The induction of ovulation by exogenous gonadotrophins is an important approach for recovering oocytes used for studies on the reproductive biology of some mammals. In the present study, pregnant mare serum gonadotrophin (PMSG) and human chorionic gonadot

微囊藻毒素对束丝藻细胞生长和抗氧化系统的影响

为从活性氧(ROS)角度探讨微囊藻毒素(MC)导致藻类细胞死亡的机理及揭示藻细胞对MC诱发的氧化胁迫的响应机制,采用50和500μg·L-1的微囊藻毒素LR(MC-LR)处理束丝藻(Aphanizomenon sp. DC01)细胞,测定了细胞生长、细胞内活性氧(ROS)含量及抗氧化系统的变化.结果表明,50μg·L-1的MC-LR处理对藻细胞的生长无显著影响,而500μg·L-1的MC-LR处理可诱导藻细胞死亡.50μg·L-1的MC-LR处理的藻细胞ROS含量在处理第2d显著高于对照;但藻细胞能通过还

月湖近代生物硅沉积测定与营养演化的动态过程

在综合了国内外实验方法基础上,完善了湖泊沉积生物硅实验室测定方法。利用此方法,首次对一富营养化小型浅水湖泊——武汉市汉阳区月湖进行了2个柱状沉积物的生物硅含量测试。结果表明,生物硅可以反映月湖的营养演化的动态过程,130a前,湖泊受到的环境压力为轻;20世纪初期,月湖生物硅沉积缓慢上升,为水体富营养化开始发生时间;20世纪30年代至50年代,月湖已成为一富营养化湖泊;20世纪60年代至80年代,月湖水质持续恶化;20世纪80年代至月湖清淤前这段时期入湖污水增多,这加速了生物硅的沉积,正是这段时期使月湖最终

微囊藻毒素-RR对烟草细胞活力及营养生理的影响

采用0.1,1.0,10.0μg/mL的微囊藻毒素-RR(MC-RR)处理烟草BY-2悬浮细胞,测定了细胞活力、细胞内蛋白质含量、可溶性糖含量、硝态氮含量及总磷含量,并且检测了酸性磷酸酶(ACP)的活力变化情况.结果表明,中、高浓度毒素处理细胞2d后,细胞活力及蛋白质含量与对照相比均显著下降.高浓度MC-RR处理降低了胞内可溶性糖的含量,暴露2d后仅为对照的45.57%;低浓度MC-RR处理在后期增加了胞内可溶性糖含量.高浓度毒素处理细胞4d后,细胞内硝态氮含量显著低于对照;中、低浓度毒素处理细胞7d后降

鳜鱼病毒结构特征与形态发生

从患流行病的鳜鱼脾脏组织超微切片中观察到大量的病毒颗粒。该完整病毒颗粒直径约 135nm± 10 ,具包膜。成熟病毒核壳体约 90nm± 5 ,包膜厚度约 18nm± 3,核壳体与包膜间的非电子致密区约有 2 7nm± 2。通过对不同发病阶段发病鳜鱼脾脏组织切片的电镜观察 ,在感染初期的鳜鱼脾脏组织观察到病毒的吸附及典型的内吞入侵方式 ,在感染中后期的脾脏组织细胞质内观察到病毒发生基质及病毒核壳、包膜形成与病毒的释放。此外 ,在染病鳜鱼的肾、心、肝、及鳃组织亦观察到相同结构的病毒粒子。回接实验证实该病毒为

鳖、蛙及鲢免疫组织中两种不同淋巴细胞的分布

国家“淡水生态和生物技术”重点实验室;湖北省自然科学基金;国家自然科学基金

鳜消化器官的组织学观察

“八五”国家科技攻关项目85-908-01-03-02

三种有机磷萃取剂对水生生物的毒性效应

通过３种有机磷萃取剂：甲基磷酸二仲辛酯（Ｐ３５０）、异辛基磷酸单异辛酯（Ｐ５０７）和磷酸二异辛酸（Ｐ２０４）对不同营养级水平的单种生物：藻类（斜生棚藻）、草履虫（尾草履虫）、类（大型）、鱼类（鱼苗）的毒性试验，结果表明：３种有机磷萃取剂的毒性效应浓度（半致死浓度或半抑制浓度）为Ｐ３５０：０．４０～９．３０ｍｇ／Ｌ，Ｐ５０７：２３．３２～１１２．００ｍｇ／Ｌ，Ｐ２０４：５９．５０～１３８．００ｍｇ／Ｌ；毒性强弱的顺序为Ｐ３５０＞Ｐ５０７＞Ｐ２０４；建议把Ｐ３５０、Ｐ５０７、Ｐ２０４在水体中的安全浓度暂分别定

钩虾体内寄生棘头虫幼虫在中国的首次报道

钩虾体内寄生棘头虫幼虫在中国的首次报道聂品（中国科学院水生生物研究所，武汉４３００７２）ＴＨＥＦＩＲＳＴＦＩＮＤＩＮＧＯＦＣＹＳＴＡＣＡＮＴＨＳＯＦＡＮＡＣＡＮＴＨＯＣＥＰＨＡＬＡＮＩＮＧＡＭＭＡＲＵＳＳＰ．ＩＮＣＨＩＮＡ￥ＮｉｃＰｉｎ（Ｉｎｓｔｉｔ...

Variables affecting melanoidins removal from molasses wastewater by coagulation/flocculation

Coagulation/flocculation process was applied in the polishing treatment of molasses wastewater on a bench-scale. Important operating variables, including coagulant type and dosage, solution pH, rapid mixing conditions as well as the type and dosage of polyeletrolytes were investigated based on the maximum removal efficiencies of chemical oxygen demand (COD) and color, residual turbidity and settling characteristics of flocs. HPSEC was utilized to evaluate the removal of molecular weight fractions of melanoidins-dominated organic compounds. Experimental results indicate that ferric chloride was the most effective among the conventional coagulants, achieving 89% COD and 98% color eliminations; while aluminum sulfate was the least effective, giving COD and color reductions of 66% and 86%, respectively. In addition to metal cations, counter-ions exert significant influence on the coagulation performance since Cl--based metal salts attained better removal efficiency than SO42--based ones at the optimal coagulant dosages. Coagulation of molasses effluent is a highly pH-dependent process, with better removal efficiency achieved at lower pH levels. Rapid mixing intensity, rather than rapid mixing time, has relatively strong influence on the settling characteristics of flocs formed. Lowering mixing intensity resulted in increasing settling rate but the accumulation of floating flocs. When used as coagulant aids, synthetic polyelectrolytes showed little effects on the improvement in organic removal. On the other hand, cationic polyacrylamide was observed to substantially enhance the settleability of flocs as compared to anionic polyacrylamide. The effects of rapid mixing conditions and polymer flocculants on the coagulation performance were discussed. (C) 2009 Elsevier B.V. All rights reserved.