Family rivalry in the testis: Retinoblastoma protein and E2F transcription factors in testicular development and adult spermatogenesis

Disorders of male reproductive health are becoming increasingly prevalent globally. These defects, ranging from decreasing sperm counts to an increasing rate of infertility and testicular cancer, have a common origin in the early phases of testicular development, but the exact mechanisms that cause them remain unknown. Testicular development and adult spermatogenesis are complex processes in which different cell types undergo mitosis, meiosis, differentiation and apoptosis. The retinoblastoma protein family and its associated E2F transcription factors are key regulators of these cellular events. In the present study, the functions of these factors in postnatal testicular development and adult spermatogenesis were explored using different animal models. In addition, a new application of flow cytometry to study testicular cell dynamics was developed. An ablation of retinoblastoma protein in mouse Sertoli cells resulted in their cell cycle re-entry in adult testes, dedifferentiation and a severe spermatogenic defect. We showed that deregulated E2F3 contributed to these changes. Our results indicated that the E2F1 transcription factor is critical for the control of apoptosis in the developing postnatal testis. In the adult testis, E2F1 controls the maintenance of the spermatogonial stem cell pool, in addition to inhibiting apoptosis of spermatocytes. In summary, this study elucidated the complex interdependencies of the RB and E2F transcription factor families in the control of postnatal testicular development and adult spermatogenesis. Furthermore, this study provided a new methodology for the analysis of testicular cells.

Evaluation of the main energy scenarios for the global energy transition

Energy scenarios are used as a tool to examine credible future states and pathways. The one who constructs a scenario defines the framework in which the possible outcomes exist. The credibility of a scenario depends on its compatibility with real world experiences, and on how well the general information of the study, methodology, and originality and processing of data are disclosed. In the thesis, selected global energy scenarios’ transparency and desirability from the society’s point of view were evaluated based on literature derived criteria. The global energy transition consists of changes to social conventions and economic development in addition to technological development. Energy solutions are economic and ethical choices due to far-reaching impacts of energy decision-making. Currently the global energy system is mostly based on fossil fuels, which is unsustainable over the long-term due to various reasons: negative climate change impacts, negative health impacts, depletion of fossil fuel reserves, resource-use conflicts with water management and food supply, loss of biodiversity, challenge to preserve ecosystems and resources for future generations, and inability of fossil fuels to provide universal access to modern energy services. Nuclear power and carbon capture and storage cannot be regarded as sustainable energy solutions due to their inherent risks and required long-term storage. The energy transition is driven by a growing energy demand, decreasing costs of renewables, modularity and scalability of renewable technologies, macroeconomic benefits of using renewables, investors’ risk awareness, renewable energy related attractive business opportunities, almost even distribution of solar and wind resources on the planet, growing awareness of the planet’s environmental status, environmental movements and tougher environmental legislation. Many of the investigated scenarios identified solar and wind power as a backbone for future energy systems. The scenarios, in which the solar and wind potentials were deployed in largest scale, met best the set out sustainability criteria. In future research, energy scenarios’ transparency can be improved by better disclosure on who has ordered the study, clarifying the funding, clearly referencing to used sources and indicating processed data, and by exploring how variations in cost assumptions and deployment of technologies influence on the outcomes of the study.

Catalogue Of the main groups of Ephemera Collection

The Ephemera Collection has been organised in groups based on the topic. The collection also includes groups formed on the basis of the publication type.

Recueil de portraits des roys et reynes de France, des princes, princesses, seigneurs et dames, et des personnes de toutes sortes de professions, dessinez à la main ou peints en miniature, et pris sur des monumens qui font connoistre les differens habillemens de chaque regne. T. I, Clovis - Saint-Louis

Collectionneur : Gaignières, Roger de (1642-1715)

[Le] général [de l'armée du salut William Bramwell] Booth [serrant la main de sa fille Mary B. Booth] : [photographie de presse] / [Agence Rol]

Référence bibliographique : Rol, 58688

22/5/20, stade Jean Bouin, le général Berdoulat [serrant la main des footballeurs des 41e R.I. et du 166e R.I.] : [photographie de presse] / [Agence Rol]

Référence bibliographique : Rol, 59353

Procès-verbal de saisie faite par les commis aux aydes [des Fermes Royales-Unies de France] sur le sieur Louis de Londre, marchand épicier à Paris, de six caisses remplies de bouteilles d'eau-de-senteur ou d'esprit-de-vin, prétendu être foiblement chargé d'odeur aromatique... et Sentence contradictoire rendue en l'Election de Paris, qui fait main-levée de ladite saisie, du 13 août 1750

[Acte. 1750-03-02. Paris]

Arrêt du grand conseil, en faveur de la communauté des huissiers du conseil, qui fait main-levée de toute saisie, délégation, transport sur les deniers-de-la bourse commune

[Acte. 1736-02-23. Paris]