Prospecção e caracterização de genes associados ao processo de indução floral em cana-de-açúcar

The northeastern region is responsible to 14.32% of sugarcane national production. This lowered contribution is due to edaphoclimatic condition. Flowering is a vital process to plant which consumes lots of energy and it culminates in a process called isoporization. This one can give in a decreasing of 60% on alcohol and water production. It may consider that cropped sugarcane has a hibrid with octaploid genome, there are varieties with a flowering standard until of non flowering. Using this natural genetic potential on different croppings of sugarcane, the aim of this work was to understand as this process occurs by the usage of subtractive approaches. The total RNA was extracted using Trizol of peaks of merisematics of croppings with induced flowering and other with late flowering. From this total RNA were built four subtractives libraries (B1- induced early flowering subtracted on late flowering not induced; B2- late flowering not induced subtracted induced early flowering; B3- induced early flowering subtracted of not induced early flowering; B02- not induced early flowering subtracted from induced early flowering) using kits Super Smart cDNA synthesis and BD Clontech kit select cDNA subtraction (Clontech). This material was clone don vector pGEM T-easy(Promega) and changed in competent cells of E.coli DH10B. Given analysis sequence was carried out a program BLASTn against database of NCBI and genome of Arabidopsis thaliana, rice and maize. Clones were grouped in 9 different classes according to function. Some factors already related as couples of flower induction were identified at different libraries. And grouped proteins with cell cycle and it controls were presents, mainly kinases proteins. Related factors to proteic sinthesis, metabolism, defence, cell communication were also given in both libraries .Some identified genes did not show similarity on database or homology with hypothesis function, and it can represents new genes to be deposited in international database. These results offers that some identified on sugarcane, classified as on factors classes, cell cycle and cell communication, trough unknown genes, can be linked with genetic changing to the flowering process found in the northeastern region

Caracterização dos genes scMUTM1 e scMUTM2 de cana-de-açúcar

Plants are organisms sessile and because of this they are susceptible to genotoxic effects due to environmental exposure such as light [including ultraviolet (UV)], heat, drought and chemicals agents. Therefore, there are differents pathways in order to detect a lesion and correct. These pathways are not well known in plants. The MutM/Fpg protein is a DNA glycosylase that is responsible for detect and correct oxidative lesions. In the sugarcane genome, it was found two possible cDNAs that had homology to this protein: scMUTM1 and scMUTM2. The aim of this work was to characterize the role of these cDNAs in plants. In order to do this, the expression level after oxidative stress was evaluated by semiquantitative RT-PCR. Another point analyzed in order to obtain the full-length gene, it was to use a sugarcane genomic library that was hybridized with both cDNAs as a probe. It was found two clones that will bought and sequenced. The promoter region was also cloned. It was obtained sequences only for scMUTM2 promoter region. The sequences obtained were divided into six groups. It was found regulatory motifs such as TATA-box, CAAT-box, oxidative stress element response and regulatory regions that response to light. The other point analyzed was to characterize the N-terminal region by PCR constructs. These constructs have deletions at 5 region. These sequences were introduce into Escherichia coli wild type strain (CC104) and double mutant (CC104mutMmutY). The results showed that proteins with deletions of scMUTM1 N-terminal region were able to complement the Fpg and MutY-glycosylase deficiency in CC104 mutMmutY reducing the spontaneous mutation frequency

Power Factor Correction Boost Converter Based on the Three-State Switching Cell

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Caracterização de dois cDNAs homológos e uma AP endonuclease em cana-de-açúcar

The genome of all organisms is subject to injuries that can be caused by endogenous and environmental factors. If these lesions are not corrected, it can be fixed generating a mutation which can be lethal to the organisms. In order to prevent this, there are different DNA repair mechanisms. These mechanisms are well known in bacteria, yeast, human, but not in plants. Two plant models Oriza sativa and Arabidopsis thaliana had the genome sequenced and due to this some DNA repair genes have been characterized. The aim of this work is to characterized two sugarcane cDNAs that had homology to AP endonuclease: scARP1 and scARP3. In silico has been done with these two sequences and other from plants. It has been observed domain conservation on these sequences, but the cystein at 65 position that is a characteristic from the redox domain in APE1 protein was not so conservated in plants. Phylogenetic relationship showed two branches, one branch with dicots and monocots sequence and the other branch with only monocots sequences. Another approach in order to characterized these two cDNAs was to construct overexpression cassettes (sense and antisense orientation) using the 35S promoter. After that, these cassettes were transferred to the binary vector pPZP211. Furthermore, previously in the laboratory was obtained a plant from nicotiana tabacum containing the overexpression cassette in anti-sense orientation. It has been observed that this plant had a slow development and problems in setting seeds. After some manual crossing, some seeds were obtained (T2) and it was analyzed the T2 segregation. The third approach used in this work was to clone the promoter region from these two cDNAs by PCR walking. The sequences obtained were analyzed using the program PLANTCARE. It was observed in these sequences some motives that may be related to oxidative stress response

Tectônica rúptil e sismicidade na área de inundação do açude do Castamhão (CE): implicações para o risco sísmico

This study describes brittle deformation and seismicity in the Castanhão Dam region, Ceará State, Brazil. This reservoir will include a hidroeletric power plant and will store about 6,7 billions m3 of water. Five main litostratigraphic unit were identified in the region: gneissic-migmatitic basement, metavolcanosedimentary sequence, granitoid plutons of Brasiliano age, Mesozoic basaltic dike swarm, and Cenozoic fluvial terraces of the Jaguaribe river. The region has experienced several faulting events that occurred at different crustal levels. Faults formed at depths less than about 12 km present left-lateral movement and are associated with epidote and quartz infillings. Faults formed at depths less than 7 km are mainly strike-slip present cataclastic rocks, fault breccia and gouge. Both fault groups form mainly NE-trendind lineaments and represent reactivation of ductile shear zones or new formed faults that cut across existing structures. Seismically-induced liquefaction fractures take place in Cenozoic terraces and indicate paleoearthquakes that may have reached at leat 6,8 MS. In short, this work indicate that the level of paleoseismicity is much greater than one observed in the instrumental record. Several faults are favourably oriented for reactivation and induced seismicity should be expected after the Castanhão Dam impoudment

A variedade da dieta é fator protetor para a pressão arterial sistólica elevada

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Genetic diversity in Egeria densa and E. najas in Jupia Reservoir, Brazil

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Influence of a cage farming on the population of the fish species Apareiodon affinis (Steindachner, 1879) in the Chavantes reservoir, Paranapanema River SP/PR, Brazil

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Fuzzy subset approach in coupled population dynamics of blowflies

This paper is a study on the population dynamics of blowflies employing a density-dependent. non-linear mathematical model and a coupled population formalism. In this Study, we investigated the coupled population dynamics applying fuzzy subsets to model the Population trajectory. analyzing demographic parameters such as fecundity, Survival, and migration. The main results suggest different possibilities in terms of dynamic behavior produced by migration in coupled Populations between distinct environments and the rescue effect generated by the connection between populations. It was possible to conclude that environmental heterogeneity can play an important role in blowfly metapopulation systems. The implications of these results for population dynamics of blowflies are discussed.

Influence of aquaculture effluents on the growth of Salvinia molesta

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Diversidade e ocorrência de peixes na área de influência da UHE Mogi Guaçu e lagoas marginais, bacia do alto rio Paraná, São Paulo, Brasil

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Population structure, feeding and reproductive aspects of Serrapinnus heterodon (Characidae, Cheirodontinae) in a Mogi Guaçu reservoir (SP), upper Paraná River basin

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Descrição de Astyanax argyrimarginatus sp. n. (Characiformes, Characidae) procedente da bacia do Rio Araguaia, Brasil

Descreve-se uma espécie nova para o gênero Astyanax Baird & Girard. A. argyrimarginatus procede da bacia do Rio Araguaia, Brasil. Esta espécie pode ser identificada por possuir uma mancha umeral negra horizontalmente ovalada, uma mancha negra no pedúnculo caudal, que se estende à extremidade dos raios caudais medianos, duas barras verticais castanho-escuras na região umeral e uma faixa lateral negra conspícua, bordeada por uma estreita faixa prateada. São discutidos outros caracteres que distinguem a espécie nova das demais espécies do gênero Astyanax portadoras de uma mancha umeral horizontalmente ovalada.

Roughness of Human Enamel Surface Submitted to Different Prophylaxis Methods

The purpose of this in vitro study was to evaluate alterations in the surface roughness and micromorphology of human enamel submitted to three prophylaxis methods. Sixty-nine caries-free molars with exposed labial surfaces were divided into three groups. Group I was treated with a rotary instrument set at a low speed, rubber clip and a mixture of water and pumice; group II with a rotary instrument set at a low speed, rubber cup and prophylaxis paste Herjos-F (Vigodent S/A Industria e Comercio, Rio de Janeiro, Brazil); and group III with sodium bicarbonate spray Profi II Ceramic (Dabi A dante Indtistrias Medico Odontologicas Ltda, Ribeirao Preto, Brazil). All procedures were performed by the same operator for 10 s, and samples were rinsed and stored in distilled water. Pre and post-treatment surface evaluation was completed using a surface profilometer (Perthometer S8P Marh, Perthen, Germany) in 54 samples. In addition, the other samples were coated with gold and examined in a scanning electron microscope (SEM). The results of this study were statistically analyzed with the paired t-test (Student), the Kruskal-Wallis test and the Dunn (5%) test. The sodium bicarbonate spray led to significantly rougher surfaces than the pumice paste. The use of prophylaxis paste showed no statistically significant difference when compared with the other methods. Based on SEM analysis, the sodium bicarbonate spray presented an irregular surface with granular material and erosions. Based on this study, it can be concluded that there was an increased enamel stuface roughness when teeth were treated with sodium bicarbonate spray when compared with teeth treated with pumice paste.

Analysis of tectonic-controlled fluvial morphology and sedimentary processes of the western Amazon Basin: an approach using satellite images and digital elevation model

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)