The production of recombinant proteins in transgenic barley grains

The grain of the self-pollinating diploid barley species offers two modes of producing recombinant enzymes or other proteins. One uses the promoters of genes with aleurone-specific expression during germination and the signal peptide code for export of the protein into the endosperm. The other uses promoters of the structural genes for storage proteins deposited in the developing endosperm. Production of a protein-engineered thermotolerant (1, 3–1, 4)-β-glucanase with the D hordein gene (Hor3–1) promoter during endosperm development was analyzed in transgenic plants with four different constructs. High expression of the enzyme and its activity in the endosperm of the mature grain required codon optimization to a C+G content of 63% and synthesis as a precursor with a signal peptide for transport through the endoplasmic reticulum and targeting into the storage vacuoles. Synthesis of the recombinant enzyme in the aleurone of germinating transgenic grain with an α-amylase promoter and the code for the export signal peptide yielded ≈1 μg⋅mg−1 soluble protein, whereas 54 μg⋅mg−1 soluble protein was produced on average in the maturing grain of 10 transgenic lines with the vector containing the gene for the (1, 3–1, 4)-β-glucanase under the control of the Hor3–1 promoter.

Catharine Waugh McCullouch papers in the Mary Earhart Dillon collection, 1869-1945

Collection primarily documents McCulloch's research on women's legal status, and her work with the Illinois Equal Suffrage Association, the National American Woman Suffrage Association, and the League of Women Voters. There is also documentation of women in the legal profession, of McCulloch's friendships with the other women suffragists and lawyers, and some biographical material. The papers contain little information about her family or social life.

Polyaromatic hydrocarbons in sediments from the Barents and White Seas

Polycyclic aromatic hydrocarbons (PAHs) are common environmental contaminants which can be derived from anthropogenic sources, such as combustion and discharges from extraction and transport, and natural processes, including leakage and erosion of fossil carbon. Natural PAH sources contribute, along with biological activities and terrestrial run-off, to the organic carbon content in sediments.The Barents Sea region is far from many anthropogenic sources of PAH, but production and trans-shipment of hydrocarbons is increasing. We present data for polycyclic aromatic hydrocarbon (PAH) concentrations in bottom sediments from 510 stations in the Barents and White Seas, and along the northern coast of Norway.

Memoirs of Marie Antoinette,

Mode of access: Internet.

Partei und Revolution /

Mode of access: Internet.

Memoirs of the private life of Marie Antoinette, to which are added personal recollections illustrative of the reigns of Louis XIV, Louis XV, and Louis XVI,

"This edition is limited to five hundred and five copies, of which this is no. 143."

Latest contemporary portraits,

"Second printing, April, 1928."

Latest contemporary portraits,

David Graham Phillips: the greatest American novelist.--Eleonora Duse.--Henri Barbusse.--Lord Bryce.--George Russell (A.E.)--Mrs. Humphry Ward.--Eugene V. Debs.--Charles Schwab: the master of Bethlehem.--Prince Peter Kropotkin.--Paul Bourget.--Thomas Hardy.--Ehrlich of "606".--Louis Sullivan: a great architect.--Eugene Fromentin: the painter writer.--Sir Herbert Tree.--Flaubert and his letters.--Hatred in art: Leon Bloy.--Three generations of Morgans.--The brothers de Goncourt and realism.--Lord Hartington.--Ambassador Bernstorff.--A talk with A.E. Housman.--Paul Deschanel.--Frederic Harrison: the last of the Victorians.--Annie Besant: rebel and reformer.--John Churton Collins.--Hyndman, the communist.--Horace Traubel.--Joseph Caillaux.

Neues Kochbuch für die deutsch-amerikanische Küche : zuverlässige und geprüfte Rezepte zur Bereitung der verschiedenen Speisen und Getränke von Backwerk, Eingemachtem u.s.w. : nach den besten dutschen und amerikanischen Koch Büchern bearbeitet, hauptsächlich nach den ausgezeichnete Kochbuch /

Recipes taken from several cookbooks, including books by Henriette Davidis and Henriette Löffler.

Ein Vermächtnis /

Mode of access: Internet.

Our old nursery rhymes.

Contains music.

Holland house.

Mode of access: Internet.

Novo diccionario da lingua portugueza e ingleza, enriquecido com grande numero de termos technicos do commercio e da industria, das sciencias e das artes e da linguagem familiar.

Added t.-p. in English.