(Table 4) Concentrations of radionuclides in waters of the Barents and Kara Seas in August 2003

A methodology of experimental simulation of state of spent nuclear fuel that occurs on the sea floor due to some catastrophes or dumping is developed. Data on long-term (more than 2000 days) experiments on estimation of 85Kr and 137Cs release rate from spent nuclear fuel (fragments of irradiated UO2 pellets) were firstly obtained; these estimates prove correctness of a hypothesis offered by us in early 1990s concerning to earlier 85Kr release (by one order of magnitude higher than that of 137Cs) as compared to other fission fragments in case of loss of integrity of fuel containment as a result of corrosion on the sea floor. A method and technique of onboard 85Kr and 137Cs sampling and extraction (as well as sampling of tritium, product of triple 235U fission) and their radiometric analysis at coastal laboratories are developed. Priority data on 85Kr background in bottom layers of the Barents and Kara Seas and 137Cs and 3H in these seas (state of 2003) are presented. Models necessary for estimation of dilution of fission products of spent nuclear fuel and their transport on the floor in accident and dumping regions are developed. An experimental method for examination of state of spent nuclear fuel on the sea floor (one expedition each 2-3 years) by 85Kr release into environment (a leak tracer) is proposed; this release is an indicator of destruction of fuel containment and release of products of spent nuclear fuel in case of 235UO2 corrosion in sea water.

Do Foreign Currency Deposits Promote or Deter Financial Development in Low-Income Countries? An Empirical Analysis of Cross-Country Data

Foreign currency deposits (FCD) are prevalent in many low-income developing countries, but their impact on bank lending has rarely been examined. An examination of cross-country data indicates that a higher proportion of FCD in total deposits is associated with growth in private credit only in inflationary circumstances (over 24 percent of the annual inflation rate). FCD can lead to a decline in private credit below this threshold level of inflation. Given that FCD exhibit persistence, deregulating them in low-income countries may do more harm than good on financial development in the long term, notably after successful containment of inflation.

Análisis Integrado de Seguridad de un accidente de SGTR en un reactor nuclear tipo PWR

El accidente de rotura de tubos de un generador de vapor (Steam Generator Tube Rupture, SGTR) en los reactores de agua a presión es uno de los transitorios más exigentes desde el punto de vista de operación. Los transitorios de SGTR son especiales, ya que podría dar lugar a emisiones radiológicas al exterior sin necesidad de daño en el núcleo previo o sin que falle la contención, ya que los SG pueden constituir una vía directa desde el reactor al medio ambiente en este transitorio. En los análisis de seguridad, el SGTR se analiza desde un punto determinista y probabilista, con distintos enfoques con respecto a las acciones del operador y las consecuencias analizadas. Cuando comenzaron los Análisis Deterministas de Seguridad (DSA), la forma de analizar el SGTR fue sin dar crédito a la acción del operador durante los primeros 30 min del transitorio, lo que suponía que el grupo de operación era capaz de detener la fuga por el tubo roto dentro de ese tiempo. Sin embargo, los diferentes casos reales de accidentes de SGTR sucedidos en los EE.UU. y alrededor del mundo demostraron que los operadores pueden emplear más de 30 minutos para detener la fuga en la vida real. Algunas metodologías fueron desarrolladas en los EEUU y en Europa para abordar esa cuestión. En el Análisis Probabilista de Seguridad (PSA), las acciones del operador se tienen en cuenta para diseñar los cabeceros en el árbol de sucesos. Los tiempos disponibles se utilizan para establecer los criterios de éxito para dichos cabeceros. Sin embargo, en una secuencia dinámica como el SGTR, las acciones de un operador son muy dependientes del tiempo disponible por las acciones humanas anteriores. Además, algunas de las secuencias de SGTR puede conducir a la liberación de actividad radiológica al exterior sin daño previo en el núcleo y que no se tienen en cuenta en el APS, ya que desde el punto de vista de la integridad de núcleo son de éxito. Para ello, para analizar todos estos factores, la forma adecuada de analizar este tipo de secuencias pueden ser a través de una metodología que contemple Árboles de Sucesos Dinámicos (Dynamic Event Trees, DET). En esta Tesis Doctoral se compara el impacto en la evolución temporal y la dosis al exterior de la hipótesis más relevantes encontradas en los Análisis Deterministas a nivel mundial. La comparación se realiza con un modelo PWR Westinghouse de tres lazos (CN Almaraz) con el código termohidráulico TRACE, con hipótesis de estimación óptima, pero con hipótesis deterministas como criterio de fallo único o pérdida de energía eléctrica exterior. Las dosis al exterior se calculan con RADTRAD, ya que es uno de los códigos utilizados normalmente para los cálculos de dosis del SGTR. El comportamiento del reactor y las dosis al exterior son muy diversas, según las diferentes hipótesis en cada metodología. Por otra parte, los resultados están bastante lejos de los límites de regulación, pese a los conservadurismos introducidos. En el siguiente paso de la Tesis Doctoral, se ha realizado un análisis de seguridad integrado del SGTR según la metodología ISA, desarrollada por el Consejo de Seguridad Nuclear español (CSN). Para ello, se ha realizado un análisis termo-hidráulico con un modelo de PWR Westinghouse de 3 lazos con el código MAAP. La metodología ISA permite la obtención del árbol de eventos dinámico del SGTR, teniendo en cuenta las incertidumbres en los tiempos de actuación del operador. Las simulaciones se realizaron con SCAIS (sistema de simulación de códigos para la evaluación de la seguridad integrada), que incluye un acoplamiento dinámico con MAAP. Las dosis al exterior se calcularon también con RADTRAD. En los resultados, se han tenido en cuenta, por primera vez en la literatura, las consecuencias de las secuencias en términos no sólo de daños en el núcleo sino de dosis al exterior. Esta tesis doctoral demuestra la necesidad de analizar todas las consecuencias que contribuyen al riesgo en un accidente como el SGTR. Para ello se ha hecho uso de una metodología integrada como ISA-CSN. Con este enfoque, la visión del DSA del SGTR (consecuencias radiológicas) se une con la visión del PSA del SGTR (consecuencias de daño al núcleo) para evaluar el riesgo total del accidente. Abstract Steam Generator Tube Rupture accidents in Pressurized Water Reactors are known to be one of the most demanding transients for the operating crew. SGTR are special transient as they could lead to radiological releases without core damage or containment failure, as they can constitute a direct path to the environment. The SGTR is analyzed from a Deterministic and Probabilistic point of view in the Safety Analysis, although the assumptions of the different approaches regarding the operator actions are quite different. In the beginning of Deterministic Safety Analysis, the way of analyzing the SGTR was not crediting the operator action for the first 30 min of the transient, assuming that the operating crew was able to stop the primary to secondary leakage within that time. However, the different real SGTR accident cases happened in the USA and over the world demonstrated that operators can took more than 30 min to stop the leakage in actual sequences. Some methodologies were raised in the USA and in Europe to cover that issue. In the Probabilistic Safety Analysis, the operator actions are taken into account to set the headers in the event tree. The available times are used to establish the success criteria for the headers. However, in such a dynamic sequence as SGTR, the operator actions are very dependent on the time available left by the other human actions. Moreover, some of the SGTR sequences can lead to offsite doses without previous core damage and they are not taken into account in PSA as from the point of view of core integrity are successful. Therefore, to analyze all this factors, the appropriate way of analyzing that kind of sequences could be through a Dynamic Event Tree methodology. This Thesis compares the impact on transient evolution and the offsite dose of the most relevant hypothesis of the different SGTR analysis included in the Deterministic Safety Analysis. The comparison is done with a PWR Westinghouse three loop model in TRACE code (Almaraz NPP), with best estimate assumptions but including deterministic hypothesis such as single failure criteria or loss of offsite power. The offsite doses are calculated with RADTRAD code, as it is one of the codes normally used for SGTR offsite dose calculations. The behaviour of the reactor and the offsite doses are quite diverse depending on the different assumptions made in each methodology. On the other hand, although the high conservatism, such as the single failure criteria, the results are quite far from the regulatory limits. In the next stage of the Thesis, the Integrated Safety Assessment (ISA) methodology, developed by the Spanish Nuclear Safety Council (CSN), has been applied to a thermohydraulical analysis of a Westinghouse 3-loop PWR plant with the MAAP code. The ISA methodology allows obtaining the SGTR Dynamic Event Tree taking into account the uncertainties on the operator actuation times. Simulations are performed with SCAIS (Simulation Code system for Integrated Safety Assessment), which includes a dynamic coupling with MAAP thermal hydraulic code. The offsite doses are calculated also with RADTRAD. The results shows the consequences of the sequences in terms not only of core damage but of offsite doses. This Thesis shows the need of analyzing all the consequences in an accident such as SGTR. For that, an it has been used an integral methodology like ISA-CSN. With this approach, the DSA vision of the SGTR (radiological consequences) is joined with the PSA vision of the SGTR (core damage consequences) to measure the total risk of the accident.

Corrección del deslizamiento de una ladera en la autovia A-4. PK. 340 en el término municipal de Marmolejo, Jaén mediante pantalla de pilotes y sistemas de drenaje

En este proyecto se describe el cálculo y diseño de una serie de medidas correctoras propuestas a raíz de un deslizamiento de ladera que tuvo lugar en noviembre de 2010 y afectó a parte de la calzada de la autovía A-4 en el P.K. 340 entre Córdoba y Jaén; principalmente en la ejecución de una pantalla de pilotes como elemento estructural de estabilización y contención del terreno, por medio del método de equilibrio límite y cálculo del módulo de reacción o coeficiente de balasto horizontal, a través del empleo de los programas Slope/W y RIDO respectivamente. ABSTRACT This project describes the calculation and design of a series of proposed corrective measures following a landslide of which took place in November 2010 and hit part of the road A-4 on the P.K. 340 between Cordoba and Jaen, mainly in the execution of a pile wall as a structural element of stabilization and containment of the terrain, through the limit equilibrium method and calculating the reaction module or horizontal ballast coefficient, through employment of the Slope/W and RIDO programs respectively.

Simulación del accidente de SGTR en un PWR-W con TRACE para distintas metodologías de análisis determinista de seguridad

A Steam Generator Tube Rupture (SGTR) in a Pressurized Water Reactor (PWR) can lead to an atmospheric release bypassing the containment via the secondary system and exiting though the Pressurized Operating Relief Valves of the affected Steam Generator. That is why SGTR historically have been treated in a special way in the different Deterministic Safety Analysis (DSA), focusing on the radioactive release more than the possibility of core damage, as it is done in the other Loss of Coolant Accidents(LOCAs).

Analysis of the operator action and the single failure criteria in a SGTR sequence using best estimate assumptions with TRACE 5.0

Steam Generator Tube Rupture (SGTR) sequences in Pressurized Water Reactors are known to be one of the most demanding transients for the operating crew. SGTR are a special kind of transient as they could lead to radiological releases without core damage or containment failure, as they can constitute a direct path from the reactor coolant system to the environment. The first methodology used to perform the Deterministic Safety Analysis (DSA) of a SGTR did not credit the operator action for the first 30 min of the transient, assuming that the operating crew was able to stop the primary to secondary leakage within that period of time. However, the different real SGTR accident cases happened in the USA and over the world demonstrated that the operators usually take more than 30 min to stop the leakage in actual sequences. Some methodologies were raised to overcome that fact, considering operator actions from the beginning of the transient, as it is done in Probabilistic Safety Analysis. This paper presents the results of comparing different assumptions regarding the single failure criteria and the operator action taken from the most common methodologies included in the different Deterministic Safety Analysis. One single failure criteria that has not been analysed previously in the literature is proposed and analysed in this paper too. The comparison is done with a PWR Westinghouse three loop model in TRACE code (Almaraz NPP) with best estimate assumptions but including deterministic hypothesis such as single failure criteria or loss of offsite power. The behaviour of the reactor is quite diverse depending on the different assumptions made regarding the operator actions. On the other hand, although there are high conservatisms included in the hypothesis, as the single failure criteria, all the results are quite far from the regulatory limits. In addition, some improvements to the Emergency Operating Procedures to minimize the offsite release from the damaged SG in case of a SGTR are outlined taking into account the offsite dose sensitivity results.

Seismic soil-interaction on earth retaining structures. Performance-based seismic design of a Retaining Wall

The design of containment walls suffering seismic loads traditionally has been realized with methods based on pseudoanalitic procedures such as Mononobe- Okabe's method, which it has led in certain occasions to insecure designs, that they have produced the ruin of many containment walls suffering the action of an earthquake. A method is proposed in this papers for the design of containment walls in different soils, suffering to the action of an earthquake, based on the Performance-Based Seismic Design.

Performance-based seismic design of a retaining wall

The design of containment walls suffering seismic loads traditionally has been realized with methods based on pseudoanalitic procedures such as Mononobe-Okabe's method, which it has led in certain occasions to insecure designs, that they have produced the ruin of many containment walls suffering the action of an earthquake. The recommendations gathered in Mononobe-Okabe's theory have been included in numerous Codes of Seismic Design. It is clear that a revision of these recommendations must be done. At present there is taking place an important review of the design methods of anti-seismic structures such as containment walls placed in an area of numerous earthquakes, by means of the introduction at the beginning of the decade of 1990 the Displacement Response Spectrum (DRS) and the Capacity Demand Diagram (CDD) that suppose an important change in the way of presenting the Elastic Response Spectrum (ERS). On the other hand in case of action of an earthquake, the dynamic characteristics of a soil have been referred traditionally to the speed of the shear waves that can be generated in a site, together with the characteristics of plasticity and damping of the soil. The Principle of the energy conservation explains why a shear upward propagating seismic wave can be amplified when travelling from a medium with high shear wave velocity (rock) to other medium with lower velocity (soil deposit), as it happened in the earthquake of Mexico of 1985. This amplification is a function of the speed gradient or of the contrast of impedances in the border of both types of mediums. A method is proposed in this paper for the design of containment walls in different soils, suffering to the action of an earthquake, based on the Performance-Based Seismic Design.

Invariantes del pensamiento en los arquitectos de Madrid. Primera década del siglo XXI. Una historia de transmisión oral

Invariantes del pensamiento en los arquitectos de Madrid. Primera década del siglo XXI. Una historia de transmisión oral es una Tesis Doctoral que parte de la elaboración de un archivo documental inédito, archivo que aglutina los testimonios de los más importantes arquitectos de Madrid presentes durante los primeros diez años del siglo XXI. Estos testimonios se recogen ordenadamente a modo de conversaciones transcritas, reflexiones puntuales y audios. La incorporación de los audios al trabajo documental permite comprobar a futuros investigadores, de manera directa, la certeza de las conclusiones o, incluso, establecer interpretaciones distintas. Los documentos sonoros son el germen de este trabajo. La Tesis Doctoral ordena e interpreta los testimonios en su apartado de ANÁLISIS/ DESARROLLO permitiendo entender a través de constantes demostraciones un hilo conductor del pensamiento en los arquitectos de Madrid. Se trata de una Tesis Doctoral que se entiende como un documento vivo, abierto y que gracias a su carácter inédito descubre matices y reflexiones de los arquitectos nunca antes recogidos en otros trabajos. Se ha conseguido reunir y ordenar por primera vez la voz y el pensamiento de los más importantes arquitectos de Madrid, muchos de ellos ya fallecidos. Se ha establecido un árbol genealógico ordenado y completo de los arquitectos de referencia indiscutibles desde el año 1939. Se ha conseguido reunir en un solo documento a los arquitectos y personajes más citados y recurrentes en el discurso de los arquitectos de Madrid, pudiendo constatar así sus referentes culturales más utilizados. Se ha descubierto y argumentado un pensamiento común dividido en cuatro conceptos: Oportunidad, Orden, Compromiso y Contención. Se produce una aproximación al arquitecto y a su pensamiento de la manera más natural y espontánea. Las grabaciones nos permitirán introducirnos, sin imposturas, no solo en el fondo, sino también en la forma de lo que se comunica, en el cómo. Una vez seleccionados los documentos sonoros más adecuados para el objetivo de este trabajo se ha procedido a su transcripción al papel. En este proceso se depura el lenguaje y se liman defectos de forma, al mismo tiempo se resumen las conversaciones y se recogen solo los comentarios más interesantes. En el proceso de transcripción, así como en la elección de las preguntas, existe una labor editorial, la aplicación de un criterio a la hora de seleccionar, resumir, corregir, completar, etc. Para abordar las conversaciones se ha recurrido a la bibliografía de referencia de cada uno de los arquitectos. Una vez transcritas las entrevistas, se establece una valoración crítica, una aproximación teórica al tema principal de la conversación. Bien puede tratarse de una reflexión sobre el arquitecto y su trabajo o sobre algunas de las opiniones o temas vertidos durante la charla. Para situar a cada uno de los arquitectos que se citan, se ha establecido un apartado genealógico completo donde cada uno se coloca en su lugar correspondiente conforme a sus apariciones en los textos principales de la historiografía reciente desde Carlos Flores hasta el año 2010. Esta Tesis Doctoral es un testigo de la diversidad de pensamientos y actitudes así como de las coincidencias. Las conversaciones mantenidas, las reflexiones y las opiniones vertidas al respecto han tocado reiteradamente muchos temas que aparecerán ordenados en el ANÁLISIS/DESARROLLO y en el apartado DESCRIPTORES. A partir de estos y otros temas genéricos, los pensamientos se aglutinan en torno a cuatro puntos que resumen las actitudes y los planteamientos conceptuales más recurrentes y coincidentes. Estos cuatro puntos definen de una manera concreta al arquitecto de Madrid. Oportunidad, Orden, Compromiso y Contención. Identidad generada a través de una historia de transmisión oral, desde los arquitectos de las primeras generaciones de posguerra hasta hoy. ABSTRACT Invariable thought in the architects of Madrid. First decade of the XXI century. A history of oral transmission is a Doctoral Thesis that starts with the development of a new documentary file, a file which brings together the testimonies of the most important architects of Madrid present during the first ten years of this century. These testimonies include conversations, punctual reflections and audio bites. Incorporating audio allows future researchers to check certain conclusions directly or even to have different interpretations. Sound bites are the seed of this work. This Doctoral Thesis orders and interprets the testimonies in the ANALYSIS / DEVELOPMENT section, through which a common thread of thought in Madrid architects can be ascertained. This doctoral thesis is meant as a living, open document, which, thanks to its unprecedented nature, discovers nuances and reflections of architects that had never been collected in previous studies. It has brought together and sorted for the first time the voice and thoughts of the most important architects of Madrid, many of them already deceased. It has established an orderly and comprehensive reference guide to the most important architects since 1939. It has brought together in one document the most cited and recurring architects and characters in the discourse of the architects of Madrid, which enables us to observe the cultural references they used the most. We have discovered and put forward a common thought divided into four concepts: Opportunity, Order, Commitment and Containment. The architect and his thoughts are revealed as naturally and spontaneously as possible. The recordings allow us to ascertain, without impositions, not only the substance but also the form of what is communicated. After selecting the most appropriate sound bites for the purpose of this work, we have proceeded to transcribe them to paper. In this process the language has been purified and formal defects have been dealt with, and at the same time the conversations have been summarized and only the most interesting comments have been kept. The transcription process and the choice of questions entails editorial work, applying a criterion when selecting, summarizing, amending, supplementing, etc. To address the conversations, the bibliographic reference of each of the architects has been used. Once the interviews have been transcribed, a critical appraisal and a theoretical approach to the main topic of conversation are established. It may be a reflection on the architect and his work or some of the views or issues discussed during the talk. In order to place each of the cited architects, a complete family tree has been devised in which each architect is situated according to his appearances in the main text of recent historiography, from Carlos Flores until 2010. This Doctoral Thesis is a witness to the diversity of thoughts, attitudes and coincidences. The conversations, reflections and opinions expressed in this regard have repeatedly touched many issues that will be sorted in the ANALYSIS/DEVELOPMENT and the KEYWORDS section. From these and other generic issues, thoughts coalesce around four points which summarize the attitudes and the most recurrent and similar conceptual approaches. These four points define the architect of Madrid in a concrete way. Opportunity, order, engagement and containment. An identity generated by a history of oral transmission, from the architects of the first post-war generations until today.

Aprovechamiento térmico de residuos estériles de carbón para generación eléctrica mediante tecnologías de combustión y gasificación eficientes y con mínimo impacto ambiental

La presente tesis doctoral, “Aprovechamiento térmico de residuos estériles de carbón para generación eléctrica mediante tecnologías de combustión y gasificación eficientes y con mínimo impacto ambiental”, desarrolla la valorización energética de los residuos del carbón, estériles de carbón, producidos durante las etapas de extracción y lavado del carbón. El sistema energético se encuentra en una encrucijada, estamos asistiendo a un cambio en el paradigma energético y, en concreto, en el sector de la generación eléctrica. Se precipita un cambio en la generación y el consumo eléctricos. Una mayor concienciación por la salud está forzando la contención y eliminación de agentes contaminantes que se generan por la utilización de combustibles fósiles de la forma en la que se viene haciendo. Aumenta la preocupación por el cambio climático y por contener en 2°C el aumento de la temperatura de la Tierra para final de este siglo, circunstancia que está impulsando el desarrollo e implantación definitiva de tecnología de control y reducción de emisiones CO2. Generar electricidad de una manera sostenible se está convirtiendo en una obligación. Esto se materializa en generar electricidad respetando el medioambiente, de una forma eficiente en la utilización de los recursos naturales y a un coste competitivo, pensando en el desarrollo de la sociedad y en el beneficio de las personas. En la actualidad, el carbón es la principal fuente de energía utilizada para generar electricidad, y su empleo presenta la forma de energía más barata para mejorar el nivel de vida de cualquier grupo y sociedad. Además, se espera que el carbón siga presente en el mix de generación eléctrica, manteniendo una significativa presencia y extrayéndose en elevadas cantidades. Pero la producción de carbón lleva asociada la generación de un residuo, estéril, que se produce durante la extracción y el lavado del mineral de carbón. Durante décadas se ha estudiado la posibilidad de utilizar el estéril y actualmente se utiliza, en un limitado porcentaje, en la construcción de carreteras, terraplenes y rellenos, y en la producción de algunos materiales de construcción. Esta tesis doctoral aborda la valorización energética del estéril, y analiza el potencial aprovechamiento del residuo para generar electricidad, en una instalación que integre tecnología disponible para minimizar el impacto medioambiental. Además, persigue aprovechar el significativo contenido en azufre que presenta el estéril para producir ácido sulfúrico (H2SO4) como subproducto de la instalación, un compuesto químico muy demandado por la industria de los fertilizantes y con multitud de aplicaciones en otros mercados. Se ha realizado el análisis de caracterización del estéril, los parámetros significativos y los valores de referencia para su empleo como combustible, encontrándose que su empleo como combustible para generar electricidad es posible. Aunque en España se lleva extrayendo carbón desde principios del siglo XVIII, se ha evaluado para un período más reciente la disponibilidad del recurso en España y la normativa existente que condiciona su aplicación en el territorio nacional. Para el período evaluado, se ha calculado que podrían estar disponibles más de 68 millones de toneladas de estéril susceptibles de ser valorizados energéticamente. Una vez realizado el análisis de la tecnología disponible y que podría considerarse para emplear el estéril como combustible, se proponen cuatro configuraciones posibles de planta, tres de ellas basadas en un proceso de combustión y una de ellas en un proceso de gasificación. Tras evaluar las cuatro configuraciones por su interés tecnológico, innovador y económico, se desarrolla el análisis conceptual de una de ellas, basada en un proceso de combustión. La instalación propuesta tiene una capacidad de 65 MW y emplea como combustible una mezcla de carbón y estéril en relación 20/80 en peso. La instalación integra tecnología para eliminar en un 99,8% el SO2 presente en el gas de combustión y en más de un 99% las partículas generadas. La instalación incorpora una unidad de producción de H2SO4, capaz de producir 18,5 t/h de producto, y otra unidad de captura para retirar un 60% del CO2 presente en la corriente de gases de combustión, produciendo 48 tCO2/h. La potencia neta de la planta es 49,7 MW. Se ha calculado el coste de inversión de la instalación, y su cálculo resulta en un coste de inversión unitario de 3.685 €/kW. ABSTRACT The present doctoral thesis, “Thermal utilisation of waste coal for electricity generation by deployment of efficient combustion and gasification technologies with minimum environmental impact”, develops an innovative waste-to-energy concept of waste coals produced during coal mining and washing. The energy system is at a dilemma, we are witnessing a shift in the energy paradigm and specifically in the field of electricity generation. A change in the generation and electrical consumption is foreseen. An increased health consciousness is forcing the containment and elimination of pollutants that are generated by the use of fossil fuels in the way that is being done. Increasing concern about climate change and to contain the rise of global temperature by 2°C by the end of this century, is promoting the development and final implementation of technology to control and reduce the CO2 emission. Electricity generation in a sustainable manner is becoming an obligation. This concept materialised in generating electricity while protecting the environment and deployment of natural resources at a competitive cost, considering the development of society and people´s benefit. Currently, coal is the main source of energy employ to generate electricity, and its use represents the most cost competitive form of energy to increase the standard of living of any group or society. Moreover, coal will keep playing a key role in the global electricity generation mix, maintaining a significant presence and being extracting in large amounts. However, coal production implies the production of waste, termed waste coal or culm in Pennsylvania anthracite extraction, produced during coal mining and coal washing activities. During the last decades, the potential use of waste coal has been studied, and currently, in a limited amount, waste coal is used in roads construction, embankments and fillings, and to produce some construction materials. This doctoral thesis evaluates the waste to energy of waste coals and assesses its potential use to generate electricity, implementing available technology to minimise the environment impact. Additionally, it pursues the significant advantage that presents sulphur content in waste coal to produce sulphuric acid (H2SO4) as a byproduct of the waste-to-energy process, a chemical compound highly demanded by the fertiliser industry and many applications in other markets. It analyses the characteristics of waste coal, and assesses the significant parameters and reference values for its use as fuel, being its fuel use for electricity generation very possible. While mining coal is taking place in Spain since the 1700s, it has been evaluated for a more recent period the waste coal available in Spain and the existing legislation that affects its application and deploy to generate electricity in the country. For the evaluation period has been calculated that may be available more than 68 million tons of waste coal that can be waste-toenergy. The potential available technology to deploy waste coal as fuel has been evaluated and assessed. After considering this, the doctoral thesis proposes four innovative alternatives of facility configuration, three of them based on a combustion process and one in a gasification process. After evaluating the four configurations for its technological, innovative and economic interest, the conceptual analysis of one of alternatives, based on a combustion process, takes place. The proposed alternative facility developed has a capacity of 65 MW, using as fuel a mixture of coal and waste coal 80/20 by weight. The facility comprises technology to remove 99.8% SO2 present in the flue gas and more than 99% of the particles. The facility includes a unit capable of producing 18.5 t/h of H2SO4, and another capture facility, removing 60% of CO2 present in the flue gas stream, producing 48 tCO2/h. The net capacity of the power station is 49.7 MW. The facility unitary cost of investment is 3,685 €/kW.

A system for functional analysis of Ebola virus glycoprotein

Ebola virus causes hemorrhagic fever in humans and nonhuman primates, resulting in mortality rates of up to 90%. Studies of this virus have been hampered by its extraordinary pathogenicity, which requires biosafety level 4 containment. To circumvent this problem, we developed a novel complementation system for functional analysis of Ebola virus glycoproteins. It relies on a recombinant vesicular stomatitis virus (VSV) that contains the green fluorescent protein gene instead of the receptor-binding G protein gene (VSVΔG*). Herein we show that Ebola Reston virus glycoprotein (ResGP) is efficiently incorporated into VSV particles. This recombinant VSV with integrated ResGP (VSVΔG*-ResGP) infected primate cells more efficiently than any of the other mammalian or avian cells examined, in a manner consistent with the host range tropism of Ebola virus, whereas VSVΔG* complemented with VSV G protein (VSVΔG*-G) efficiently infected the majority of the cells tested. We also tested the utility of this system for investigating the cellular receptors for Ebola virus. Chemical modification of cells to alter their surface proteins markedly reduced their susceptibility to VSVΔG*-ResGP but not to VSVΔG*-G. These findings suggest that cell surface glycoproteins with N-linked oligosaccharide chains contribute to the entry of Ebola viruses, presumably acting as a specific receptor and/or cofactor for virus entry. Thus, our VSV system should be useful for investigating the functions of glycoproteins from highly pathogenic viruses or those incapable of being cultured in vitro.

Surface geochemistry of the clay minerals

Clay minerals are layer type aluminosilicates that figure in terrestrial biogeochemical cycles, in the buffering capacity of the oceans, and in the containment of toxic waste materials. They are also used as lubricants in petroleum extraction and as industrial catalysts for the synthesis of many organic compounds. These applications derive fundamentally from the colloidal size and permanent structural charge of clay mineral particles, which endow them with significant surface reactivity. Unraveling the surface geochemistry of hydrated clay minerals is an abiding, if difficult, topic in earth sciences research. Recent experimental and computational studies that take advantage of new methodologies and basic insights derived from the study of concentrated ionic solutions have begun to clarify the structure of electrical double layers formed on hydrated clay mineral surfaces, particularly those in the interlayer region of swelling 2:1 layer type clay minerals. One emerging trend is that the coordination of interlayer cations with water molecules and clay mineral surface oxygens is governed largely by cation size and charge, similarly to a concentrated ionic solution, but the location of structural charge within a clay layer and the existence of hydrophobic patches on its surface provide important modulations. The larger the interlayer cation, the greater the influence of clay mineral structure and hydrophobicity on the configurations of adsorbed water molecules. This picture extends readily to hydrophobic molecules adsorbed within an interlayer region, with important implications for clay–hydrocarbon interactions and the design of catalysts for organic synthesis.

Nuclear waste forms for actinides

The disposition of actinides, most recently 239Pu from dismantled nuclear weapons, requires effective containment of waste generated by the nuclear fuel cycle. Because actinides (e.g., 239Pu and 237Np) are long-lived, they have a major impact on risk assessments of geologic repositories. Thus, demonstrable, long-term chemical and mechanical durability are essential properties of waste forms for the immobilization of actinides. Mineralogic and geologic studies provide excellent candidate phases for immobilization and a unique database that cannot be duplicated by a purely materials science approach. The “mineralogic approach” is illustrated by a discussion of zircon as a phase for the immobilization of excess weapons plutonium.

Construction of an improved mycoinsecticide overexpressing a toxic protease.

Mycoinsecticides are being used for the control of many insect pests as an environmentally acceptable alternative to chemical insecticides. A key aim of much recent work has been to increase the speed of kill and so improve commercial efficacy of these biocontrol agents. This might he achieved by adding insecticidal genes to the fungus, an approach considered to have enormous potential for the improvement of biological pesticides. We report here the development of a genetically improved entomopathogenic fungus. Additional copies of the gene encoding a regulated cuticle-degrading protease (Pr1) from Metarhizium anisopliae were inserted into the genome of M. anisopliae such that Pr1 was constitutively overproduced in the hemolymph of Manduca sexta, activating the prophenoloxidase system. The combined toxic effects of Pr1 and the reaction products of phenoloxidase caused larvae challenged with the engineered fungus to exhibit a 25% reduction in time of death and reduced food consumption by 40% compared to infections by the wild-type fungus. In addition, infected insects were rapidly melanized, and the resulting cadavers were poor substrates for fungal sporulation. Thus, environmental persistence of the genetically engineered fungus is reduced, thereby providing biological containment.

Gut-specific transcriptional regulatory elements of the carboxypeptidase gene are conserved between black flies and Drosophila.

Millions of people die every year in the tropical world from diseases transmitted by hematophagous insects. Failure of conventional containment measures emphasizes the need for additional approaches, such as transformation of vector insects with genes that restrict vectorial capacity. The availability of an efficient promoter to drive foreign genes in transgenic insects is a necessary tool to test the feasibility of such approach. Here we characterize the putative promoter region of a black fly midgut carboxypeptidase gene and show that these sequences correctly direct the expression of a beta-glucuronidase reporter in Drosophila melanogaster. By histochemical staining and mRNA analysis, we found that the gene is expressed strongly and gut-specifically in the transgenic Drosophila. This gut-specific black fly carboxypeptidase promoter provides a valuable tool for the study of disease vectors.