B2 肾上腺素受体多肽16 位基因型与夜间哮喘表现型的关系

目的　探讨B2 肾上腺素受体(B2AR) 16、27 位基因多态性与夜间哮喘表现型的关系。方法　以最大呼 气流速(PEFR ) 为标准, 将49 例哮喘患者分为夜间哮喘组(25 例) 和非夜间哮喘组(24 例)。用PCR 产物直接测序确 定B2AR 16、27 位基因型分布, 以及分析两个位点各种基因型与两组病例PEFR、第一秒用力呼气量(FEV 1) 以及用药 情况之间的关系。结果　以PEFR 为标准, 夜间哮喘组PEFR 在夜间平均下降33. 6% , 非夜间哮喘组下降7. 0% , 二 者差异显著(P < 0. 001)。夜间哮喘组和非夜间哮喘组(白天) 基础FEV 1 分别为73. 7 % 和85. 8 % , 也具有显著性差 异(P < 0. 001)。Gly16 的等位基因频率在夜间哮喘组56. 0% 明显较非夜间哮喘组22. 9% 高(P < 0. 05) , Gly16 集中 分布于夜间哮喘组。27 位点的多态性在两组间无显著性差异。结论　B2AR Gly16 基因型与夜间哮喘可能有关系。

猴脑线粒体DNA提取及限制性内切酶分析

用冷碱法从2只恒河猴和1只食蟹猴的脑组织中提取mtDNA, 最后的得率大约 为0.7μg mtDNA/g脑组织, 是肝脏组织得率的1/3左右。经16 种限制性内切酶分 析, 并与来自同一个体肝脏组织的mtDNA比较, 结果进一步证实, mtDNA无组织 特异性。对12岁以上老年猴脑mtDNA的分 析表明, 在衰老中, 动物mtDNA的序列 可能没有变化, 甲基化程度也无显著增高。图2参4

mtDNA Data Indicate a Single Origin for Dogs South of Yangtze River, Less Than 16,300 Years Ago, from Numerous Wolves

There is no generally accepted picture of where, when, and how the domestic dog originated. Previous studies of mitochondrial DNA (mtDNA) have failed to establish the time and precise place of origin because of lack of phylogenetic resolution in the so fa

BOBLME Oceanographic Working Group Meeting, Perth, Australia, July 12-16, 2010

Overview from member countries of oceanography and large scale dynamic processes affecting the Bay of Bengal

Report of the training programme on Essential Ecosystem Approach to Fisheries Management (EAFM): developing capacity in the EAFM, Chennai, India, 11-16 August, 2014

Participants were exposed to concepts and information about EAFM using a structured, participatory method of delivery. The learning strategy involved specifically designed exercises, using real examples, to consolidate learning. Daily monitoring and reviews were conducted together with pre-and post-course assessment.

川金丝猴(Rhinopithecus roxellanae)月经周期促黄体素(LH)的分泌水平

用改进的LH体外生物测定法, 测定了川金丝猴两个月经周期的晨尿LH含量。结果表明LH峰分别出现在各月经周期的第16天和11天。LH峰的平均水平为100.0±9.0miu/ml尿[以人促黄激素(hLH)69/104作为标准], 是滤泡期LH平均水平的6倍, 黄体期LH平均水平的4倍。川金丝猴月经周期LH的分泌情况与人和猕猴的极为相似。表1图3

Interaction between human interleukin-16 and CD4 receptor of HIV-1

AIM: To study the interaction between human interleukin-16 (IL-16) and the receptor CD4 (T-lymphocyte differentiation antigen) of human immunodeficiency virus type 1 (HIV-1). METHODS: Two structurally con served regions (SCRs) of human IL-16 were built by the SYBYL/Biopolymer module using the corresponding transmembrane (TM) domain of human interleukin-1 (HIL-4) and HIL-2 as the templates. The coordinates for amino-terminal residue sequence, carboxyl-terminal residue sequences, and cytoplasm loops were generated using Biopolymer's LOOP SEARCH algorithm. RESULTS: HIL-16 first formed a homodimer, then contacted with CD4 dimer further forming a dimeric complex. Subsequently, the dimeric complex constructed the tetrameric complex by two disulfide bridges between the cysteines of HIL-16 (Cys31-Cys31). CONCLUSION: The interaction model is useful to propose the action mechanism of HIL-16 and is beneficial for rational designing of novel anti-HIV drugs.