Desenvolvimento de métodos para quantificação de benzilpenicilina em medicamentos veterinários e seus resíduos em leite bovino e caprino por UFLC-DAD

The Benzylpenicillin (PENG) have been as the active ingredient in veterinary medicinal products, to increase productivity, due to its therapeutic properties. However, one of unfortunate quality and used indiscriminately, resulting in residues in foods exposed to human consumption, especially in milk that is essential to the diet of children and the ageing. Thus, it is indispensable to develop new methods able to detect this waste food, at levels that are toxic to human health, in order to contribute to the food security of consumers and collaborate with regulatory agencies in an efficient inspection. In this work, were developed methods for the quality control of veterinary drugs based on Benzylpenicillin (PENG) that are used in livestock production. Additionally, were validated methodologies for identifying and quantifying the antibiotic residues in milk bovine and caprine. For this, the analytical control was performed two steps. At first, the groups of samples of medicinal products I, II, III, IV and V, individually, were characterized by medium infrared spectroscopy (4000 – 600 cm-1). Besides, 37 samples, distributed in these groups, were analyzed by spectroscopy in the ultraviolet and near infrared region (UV VIS NIR) and Ultra Fast Liquid Chromatograph coupled to linear arrangement photodiodes (UFLC-DAD). The results of the characterization indicated similarities, between PENG and reference standard samples, primarily in regions of 1818 to 1724 cm-1 of ν C=O that shows primary amides features of PENG. The method by UFLC-DAD presented R on 0.9991. LOD of 7.384 × 10-4 μg mL-1. LOQ of 2.049 × 10-3 μg mL-1. The analysis shows that 62.16% the samples presented purity ≥ 81.21%. The method by spectroscopy in the UV VIS NIR presented medium error ≤ 8 – 12% between the reference and experimental criteria, indicating is a secure choice for rapid determination of PENG. In the second stage, was acquiring a method for the extraction and isolation of PENG by the addition of buffer McIlvaine, used for precipitation of proteins total, at pH 4.0. The results showed excellent recovery values PENG, being close to 92.05% of samples of bovine milk (method 1). While samples of milk goats (method 2) the recovery of PENG were 95.83%. The methods for UFLC-DAD have been validated in accordance with the maximum residue limit (LMR) of 4 μg Kg-1 standardized by CAC/GL16. Validation of the method 1 indicated R by 0.9975. LOD of 7.246 × 10-4 μg mL-1. LOQ de 2.196 × 10-3 μg mL-1. The application of the method 1 showed that 12% the samples presented concentration of residues of PENG > LMR. The method 2 indicated R by 0.9995. LOD 8.251 × 10-4 μg mL-1. LOQ de 2.5270 × 10-3 μg mL-1. The application of the method showed that 15% of the samples were above the tolerable. The comparative analysis between the methods pointed better validation for LCP samples, because the reduction of the matrix effect, on this account the tcalculs < ttable, caused by the increase of recovery of the PENG. In this mode, all the operations developed to deliver simplicity, speed, selectivity, reduced analysis time and reagent use and toxic solvents, particularly if compared to the established methodologies.

Desenvolvimento de métodos para quantificação de benzilpenicilina em medicamentos veterinários e seus resíduos em leite bovino e caprino por UFLC-DAD

The Benzylpenicillin (PENG) have been as the active ingredient in veterinary medicinal products, to increase productivity, due to its therapeutic properties. However, one of unfortunate quality and used indiscriminately, resulting in residues in foods exposed to human consumption, especially in milk that is essential to the diet of children and the ageing. Thus, it is indispensable to develop new methods able to detect this waste food, at levels that are toxic to human health, in order to contribute to the food security of consumers and collaborate with regulatory agencies in an efficient inspection. In this work, were developed methods for the quality control of veterinary drugs based on Benzylpenicillin (PENG) that are used in livestock production. Additionally, were validated methodologies for identifying and quantifying the antibiotic residues in milk bovine and caprine. For this, the analytical control was performed two steps. At first, the groups of samples of medicinal products I, II, III, IV and V, individually, were characterized by medium infrared spectroscopy (4000 – 600 cm-1). Besides, 37 samples, distributed in these groups, were analyzed by spectroscopy in the ultraviolet and near infrared region (UV VIS NIR) and Ultra Fast Liquid Chromatograph coupled to linear arrangement photodiodes (UFLC-DAD). The results of the characterization indicated similarities, between PENG and reference standard samples, primarily in regions of 1818 to 1724 cm-1 of ν C=O that shows primary amides features of PENG. The method by UFLC-DAD presented R on 0.9991. LOD of 7.384 × 10-4 μg mL-1. LOQ of 2.049 × 10-3 μg mL-1. The analysis shows that 62.16% the samples presented purity ≥ 81.21%. The method by spectroscopy in the UV VIS NIR presented medium error ≤ 8 – 12% between the reference and experimental criteria, indicating is a secure choice for rapid determination of PENG. In the second stage, was acquiring a method for the extraction and isolation of PENG by the addition of buffer McIlvaine, used for precipitation of proteins total, at pH 4.0. The results showed excellent recovery values PENG, being close to 92.05% of samples of bovine milk (method 1). While samples of milk goats (method 2) the recovery of PENG were 95.83%. The methods for UFLC-DAD have been validated in accordance with the maximum residue limit (LMR) of 4 μg Kg-1 standardized by CAC/GL16. Validation of the method 1 indicated R by 0.9975. LOD of 7.246 × 10-4 μg mL-1. LOQ de 2.196 × 10-3 μg mL-1. The application of the method 1 showed that 12% the samples presented concentration of residues of PENG > LMR. The method 2 indicated R by 0.9995. LOD 8.251 × 10-4 μg mL-1. LOQ de 2.5270 × 10-3 μg mL-1. The application of the method showed that 15% of the samples were above the tolerable. The comparative analysis between the methods pointed better validation for LCP samples, because the reduction of the matrix effect, on this account the tcalculs < ttable, caused by the increase of recovery of the PENG. In this mode, all the operations developed to deliver simplicity, speed, selectivity, reduced analysis time and reagent use and toxic solvents, particularly if compared to the established methodologies.

Desenvolvimento e validação de testes de fluxo lateral para a detecção de cultivares geneticamente modificados e de aflatoxinas em produtos agrícolas

The expansion of cultivated areas with genetically modified crops (GM) is a worldwide phenomenon, stimulating regulatory authorities to implement strict procedures to monitor and verify the presence of GM varieties in agricultural crops. With the constant growing of plant cultivating areas all over the world, consumption of aflatoxin-contaminated food also increased. Aflatoxins correspond to a class of highly toxic contaminants found in agricultural products that can have harmful effects on human and animal health. Therefore, the safety and quality evaluation of agricultural products are important issues for consumers. Lateral flow tests (strip tests) is a promising method for the detection both proteins expressed in GM crops and aflatoxins-contaminated food samples. The advantages of this technique include its simplicity, rapidity and cost-effective when compared to the conventional methods. In this study, two novel and sensitive strip tests assay were developed for the identification of: (i) Cry1Ac and Cry8Ka5 proteins expressed in GM cotton crops and; (ii) aflatoxins from agricultural products. The first strip test was developed using a sandwhich format, while the second one was developed using a competitive format. Gold colloidal nanoparticles were used as detector reagent when coated with monoclonal antibodies. An anti-species specific antibody was sprayed at the nitrocellulose membrane to be used as a control line. To validate the first strip test, GM (Bollgard I® e Planta 50- EMBRAPA) and non-GM cotton leaf (Cooker 312) were used. The results showed that the strip containing antibodies for the identification of Cry1Ac and Cry8Ka5 proteins was capable of correctly distinguishing between GM samples (positive result) and non-GM samples (negative result), in a high sensitivity manner. To validate the second strip test, artificially contaminated soybean with Aspergillus flavus (aflatoxin-producing fungus) was employed. Food samples, such as milk and soybean, were also evaluated for the presence of aflatoxins. The strip test was capable to distinguish between samples with and without aflatoxins samples, at a sensitivity concentration of 0,5 μg/Kg. Therefore, these results suggest that the strip tests developed in this study can be a potential tool as a rapid and cost-effective method for detection of insect resistant GM crops expressing Cry1Ac and Cry8Ka5 and aflatoxins from food samples.

Desenvolvimento e validação de testes de fluxo lateral para a detecção de cultivares geneticamente modificados e de aflatoxinas em produtos agrícolas

The expansion of cultivated areas with genetically modified crops (GM) is a worldwide phenomenon, stimulating regulatory authorities to implement strict procedures to monitor and verify the presence of GM varieties in agricultural crops. With the constant growing of plant cultivating areas all over the world, consumption of aflatoxin-contaminated food also increased. Aflatoxins correspond to a class of highly toxic contaminants found in agricultural products that can have harmful effects on human and animal health. Therefore, the safety and quality evaluation of agricultural products are important issues for consumers. Lateral flow tests (strip tests) is a promising method for the detection both proteins expressed in GM crops and aflatoxins-contaminated food samples. The advantages of this technique include its simplicity, rapidity and cost-effective when compared to the conventional methods. In this study, two novel and sensitive strip tests assay were developed for the identification of: (i) Cry1Ac and Cry8Ka5 proteins expressed in GM cotton crops and; (ii) aflatoxins from agricultural products. The first strip test was developed using a sandwhich format, while the second one was developed using a competitive format. Gold colloidal nanoparticles were used as detector reagent when coated with monoclonal antibodies. An anti-species specific antibody was sprayed at the nitrocellulose membrane to be used as a control line. To validate the first strip test, GM (Bollgard I® e Planta 50- EMBRAPA) and non-GM cotton leaf (Cooker 312) were used. The results showed that the strip containing antibodies for the identification of Cry1Ac and Cry8Ka5 proteins was capable of correctly distinguishing between GM samples (positive result) and non-GM samples (negative result), in a high sensitivity manner. To validate the second strip test, artificially contaminated soybean with Aspergillus flavus (aflatoxin-producing fungus) was employed. Food samples, such as milk and soybean, were also evaluated for the presence of aflatoxins. The strip test was capable to distinguish between samples with and without aflatoxins samples, at a sensitivity concentration of 0,5 μg/Kg. Therefore, these results suggest that the strip tests developed in this study can be a potential tool as a rapid and cost-effective method for detection of insect resistant GM crops expressing Cry1Ac and Cry8Ka5 and aflatoxins from food samples.

Renovação da certificação do referencial BRC, aplicação do código de ética laboral ETI – Ethical Trading Initiative e do referencial específico da Walmart na central fruteira O Melro.OP

A crescente preocupação com a segurança alimentar por parte das empresas do setor, assim como a segurança e o bem estar dos trabalhadores cresce com o alargamento do mercado de exportação e importação de produtos. As exigências de muitos retalhistas e grossistas europeus reforçam a necessidade de implementação de referenciais específicos. Este trabalho tem por base a atualização da norma British Retail Consortium (BRC) para a sétima versão, implementação do referencial Ethical Trading Initiative (ETI) e Walmart na central fruteira O Melro.OP. Todos os referenciais foram analisados, de forma a compreender as melhores metodologias para o cumprimento dos requisitos exigidos, tendo sido consultada a legislação aplicável relevante aos vários setores, recursos humanos, ética, segurança e higiene no trabalho e ambiente. Procedeu-se à elaboração das check lists para cada referencial e a uma auditoria prévia com o objetivo de avaliar a situação atual da empresa. Elaboraram-se relatórios e planos de ação indicando as alterações a ter em conta para a implementação e atualização dos referenciais e toda a documentação associada. Posteriormente foi feita uma auditoria interna de modo a confirmar que o plano de ação da auditoria prévia foi aplicado, tendo-se elaborado o relatório da auditoria e novos planos de ação para futura melhoria e obtenção das respetivas certificações. Com a implementação destas certificações para os referenciais BRC, ETI e Walmart a empresa pode obter uma maior visibilidade no mercado, aumentado assim o seu leque de clientes.

Knowledge generation in agricultura research

No decorrer das últimas décadas, a agropecuária brasileira tem desempenhado um papel importante no mercado internacional, em resposta às crescentes demandas globais por produtos, serviços e segurança alimentar. Essa conquista foi estimulada, em grande parte, pela capacidade de geração de conhecimento e ações promovidas pelos institutos de ciência e tecnologia. Este artigo visa a descrever o modelo de geração do conhecimento na pesquisa agropecuária, assumindo que o ciclo do conhecimento inclui a captura, internalização e compartilhamento do conhecimento, mediante práticas informais e formais no ambiente de trabalho e nas redes de relacionamento, pessoais e institucionais. Precedida por ampla revisão teórica, a pesquisa é baseada em um estudo multicasos em três institutos de ciência e tecnologia, com foco na pesquisa agropecuária, com abordagem quali-quantitativa, instrumentalizada por entrevistas semiestruturadas aos pesquisadores seniores e aplicação de questionários em uma amostra de 410 pesquisadores com título de mestres e doutores em áreas relacionadas à pesquisa agropecuária. Os resultados indicam um modelo de geração do conhecimento em institutos de pesquisa agropecuária voltados à inovação, que tem origem na captura de ideias sobre como solucionar um problema com uso da competência tecnológica desenvolvida, por meio da elaboração do projeto de pesquisa.

Knowledge generation in agricultura research

No decorrer das últimas décadas, a agropecuária brasileira tem desempenhado um papel importante no mercado internacional, em resposta às crescentes demandas globais por produtos, serviços e segurança alimentar. Essa conquista foi estimulada, em grande parte, pela capacidade de geração de conhecimento e ações promovidas pelos institutos de ciência e tecnologia. Este artigo visa a descrever o modelo de geração do conhecimento na pesquisa agropecuária, assumindo que o ciclo do conhecimento inclui a captura, internalização e compartilhamento do conhecimento, mediante práticas informais e formais no ambiente de trabalho e nas redes de relacionamento, pessoais e institucionais. Precedida por ampla revisão teórica, a pesquisa é baseada em um estudo multicasos em três institutos de ciência e tecnologia, com foco na pesquisa agropecuária, com abordagem quali-quantitativa, instrumentalizada por entrevistas semiestruturadas aos pesquisadores seniores e aplicação de questionários em uma amostra de 410 pesquisadores com título de mestres e doutores em áreas relacionadas à pesquisa agropecuária. Os resultados indicam um modelo de geração do conhecimento em institutos de pesquisa agropecuária voltados à inovação, que tem origem na captura de ideias sobre como solucionar um problema com uso da competência tecnológica desenvolvida, por meio da elaboração do projeto de pesquisa.

Relatório de Estágio Profissional. Gestão de Alimentos e Bebicas (F&B)

O presente relatório foi desenvolvido, após realizar de um estágio no departamento de F&B, com uma carga horária de 480 horas, em três meses, sendo supervisionada pela assistente do F&B. O objetivo desse estágio foi observar com um olhar crítico, as práticas do funcionamento do Departamento de F&B. Este abrange as seguintes áreas: Restaurante, Bar, Cozinha e Economato. Também permitiu conhecer as práticas da Administração dos Recursos Humanos, Planeamento e Gestão de Eventos; Gestão e Controlo de Vendas bem com as Políticas Comerciais/ Marketing. Durante a apresentação deste relatório serão retratadas todas as seções acima transcritas, reconhecendo os staffs, equipamentos e organização, demonstrando a importância, as operações, as responsabilidades e competências de cada seção neste departamento. Este estágio contribuiu para um melhor enriquecimento dos meus conhecimentos, aumentando assim as minhas capacidades práticas do formando.

Avaliação da viabilidade da aplicação de técnicas biomoleculares na autenticação de produtos alimentares

A carne continua a ser a fonte proteica mais comum no quotidiano das pessoas. Além disso, os produtos cárneos processados apresentam-se como uma mais-valia nas suas vidas agitadas. Este tipo de produto torna difícil a diferenciação das carnes utilizadas na sua confecção, sendo por isso propícios a adulteração. A Reacção em Cadeia da Polimerase (PCR) tem ganho cada vez mais importância nos laboratórios de biologia molecular, revelando-se uma técnica de análise rápida, sensível e altamente específica na identificação de espécies em produtos alimentares. No entanto, vários factores podem interferir com o processo de amplificação, pelo que alguns cuidados devem ser implementados desde a aquisição da amostra a analisar, ao seu acondicionamento e posterior extração de ADN. Existem inúmeros protocolos de extração de ADN, devendo para cada estudo avaliar-se e optar-se pelo mais adequado, considerando a finalidade estabelecida para a amostra extraída. O trabalho laboratorial apresentado nesta dissertação baseou-se em três etapas principais. Inicialmente, avaliaram-se diferentes protocolos de extração de ADN, utilizando-se amostras de carne adquiridas num talho. Entre os protocolos testados, o método de Brometo de Cetil-Trimetil-Amónio (CTAB) modificado foi o que permitiu obter amostras de ADN com maior concentração e elevado nível de pureza. Posteriormente, foram testados e optimizados diferentes protocolos de amplificação, por PCR em tempo real, para a detecção das espécies Bos taurus (vaca), Sus scrofa (porco), Equus caballus (cavalo) e Ovis aries (ovelha). Foram empregues primers específicos de espécie para a detecção de genes mitocondriais e genómicos, consoante cada protocolo. Para o caso concreto do porco, foi efectuada a avaliação de dois protocolos, singleplex com EvaGreen® e tetraplex com AllHorse, para possível aplicação dos mesmos na sua quantificação. Os resultados demonstraram elevada especificidade e sensibilidade das reacções para esta espécie, permitindo a sua detecção até um limite de 0,001 ng e 0,1%, respectivamente. Somente a primeira metodologia se mostrou adequada para quantificação. Por último, as metodologias sugeridas foram aplicadas com sucesso na análise de 4 amostras comerciais de hambúrgueres, tendo-se verificado a consistência da rotulagem em todos os casos, no que concerne a composição em termos de espécies animais. O interesse de trabalhos neste âmbito recai na importância da autenticidade dos rótulos de produtos alimentares, principalmente nos produtos cárneos, para segurança dos consumidores e salvaguarda dos produtores.

Speciation analysis of Arsenic in fish and rice samples using liquid chromatography-inductively coupled plasma mass spectrometry

Chemical speciation in foodstuffs is of uttermost importance since it is nowadays recognized that both toxicity and bioavailability of an element depend on the chemical form in which the element is present. Regarding arsenic, inorganic species are classified as carcinogenic while organic arsenic, such as arsenobetaine (AsB) or arsenocholine (AsC), is considered less toxic or even non-toxic. Coupling a High Performance Liquid Chromatographer (HPLC) with an Inductively Coupled Plasma Mass Spectrometer (ICP-MS) combines the power of separation of the first with the selectivity and sensitivity of the second. The present work aims at developing a method, using HPLC-ICP-MS technique, to identify and quantify the chemical species of arsenic present in two food matrices, rice and fish. Two extraction methods, ultrasound and microwave, and different settings were studied. The best method was chosen based on recovery percentages. To ensure that no interconversion of species was occurring, individual spikes of each species of arsenic were made in both matrices and recovery rates were calculated. To guaranty accurate results reference material BCR-627 TUNA FISH, containing certified values for AsB and DMA, was analyzed. Chromatographic separation was achieved using an anion exchange column, HAMILTON-PRP X-100, which allowed to separate the four arsenic species for which standards were available (AsB, dimethylarsenic (DMA), arsenite (AsIII), arsenate (AsV). The mobile phase was chosen based on scientific literature and adjusted to laboratory conditions. Different gradients were studied. As a result we verified that the arsenic species present in both matrices were not the same. While in fish 90% of the arsenic present was in the form of arsenobetaine, in rice 80% of arsenic was present as DMA and 20% as inorganic arsenic.

Design of experiments to optimized the determination of TDIs in agglomerated cork stoppers

Isocyanates are included into a class with an extreme commercial importance because their use in the manufacture of polyurethanes. Polyurethanes are used in several applications such as adhesives, coatings, foams, thermoplastics resins, printing inks, foundry moulds and rubbers. Agglomerated cork stoppers are currently used for still wines, semi-sparkle and gaseous wines, beer and cider. Methylene diphenyl diisocyanate (MDI) is presently the isocyanate used in the production of polyurethane based adhesive in use due to its lowest toxicity comparing with toluene diisocyanate (TDI) previously employed. However, free monomeric TDI or MDI, depending on the based polyurethane, can migrate from agglomerated cork stoppers to beverages therefore it needs to be under control. The presence of these compounds are usually investigated by HPLC with Fluorescence or UV-Vis detector depending on the derivatising agent. Ultra Performance Liquid Chromatography with Diode Array Detector (UPLC-DAD) method is replacing HPLC. The objective of this study is to determine which method is better to analyze isocyanates from agglomerated cork stoppers, essentially TDI to quantify its monomer. A Design of Experiments (DOE) with three factors, column temperature, flow and solvent, at two levels was done. Eight experiments with three replications and two repetitions were developed. Through an ANOVA the significance of the factors was evaluated and the best level’s factors were selected. As the TDI has two isomers and in this method these two isomers were not always separated an ANOVA with results of resolution between peaks was performed. The Design of Experiments reveals to be a suitable statistical tool to determine the best conditions to quantified free isocyanates from agglomerated cork stoppers to real foodstuff. The best level’s factors to maximize area was column temperature at 30ºC, flow to 0,3 mL/min and solvent 0,1% Ammonium Acetate, to maximize resolution was the same except the solvent that was 0,01% Ammonium Acetate.

Rede de conservação on farm para fruteiras nativas da fitofisionomia floresta com araucária: levantamento de informações

Currently, the biodiversity is considered as a powerful food security strategy, ecological and economical for humanity. Brazil is one of the main centers of genetic diversity of native fruit in the world. However, little is known about most of these species. In southwestern Paraná region, this diversity can be found, however, due to human action to increase genetic erosion, it is losing genotypes with potential for use. Thus, the conservation of genetic resources is essential for reduction strategies for damage caused to the environment and the lack of tech-nical information to increase the use of them. This study aimed to obtain information for cre-ating on farm net conservation in four citties this region. This study was carried out in rural properties from Dois Vizinhos, Itapejara D’Oeste, Verê and São Jorge D'Oeste citties, Paraná State, Brazil. It was action plan was established with the rural communities through gathering information with agents considered key in the process, it seeking the greatest number of farm-ers who had their properties in the native fruits as pitanga, jabuticaba, uvaia, cereja-do-mato, guabiroba, guabiju, sete capote, goiaba serrana, araça amarelo e vermelho trees. Semi-structured questionnaire was applied, which concerned issues of presence, handling and use of Myrtaceae fruit trees on their properties and informed consent term. There was a survey of the number and native fruits present in each property. The characterization of each household in terms of diversity handled and used in native fruit was performed. It was realized the soil col-lect in 200 properties with the presence of at least some native fruit tree naturally occurring, in order to determine the preference of the species for the chemical characteristics of the soil. The four citties have native fruits trees in quantity and diversity for the creation of on farm net conservation, with farmers demonstrating knowledge of their role as guardians of this heritage of humanity.

Preparação para a implementação do referencial BRC numa indústria de abate e transformação de aves

Atualmente a segurança e qualidade alimentar surge como umas das maiores preocupações da indústria alimentar, isto deve-se a uma maior exigência dos consumidores, ao aumento do receio devido a recentes crises de segurança alimentar e consequentemente ao maior grau de exigência do quadro regulamentar aplicável ao sector Agroindustrial. Este facto levou ao aparecimento de vários referenciais normativos, entre eles aparece a norma BRC, uma norma reconhecida internacionalmente. A implementação e certificação de um sistema de gestão de segurança alimentar reconhecido internacionalmente é uma das metodologias mais eficazes de as empresas do sector alimentar garantirem a eficácia dos processos relacionados com a qualidade e segurança alimentar aumentando assim a confiança dos consumidores e clientes e consequentemente a sua competitividade num mercado global. Foi nesta base que foi desenvolvido o presente projeto, que consistiu na preparação de uma pequena e média empresa de abate e transformação de aves para a implementação do referencial BRC – Global Standard for food safety – Issue 6, July 2011, British Retail Consortium, London A preparação para implementação deste referencial foi elaborada em diferentes fases: numa primeira fase procedeu-se à realização de uma auditoria de diagnóstico, de modo a avaliar os procedimentos em comparação com os requisitos da norma. Verificaram-se nesta fase 45 não conformidades, com base nas quais foi elaborado um plano de ações e alterações em concordância das necessidades identificadas de forma a cumprir os requisitos da norma. O sistema de gestão BRC na empresa em estudo encontra-se atualmente totalmente planeado e estruturado estando igualmente definida a estrutura documental necessária ao cumprimento dos requisitos da norma. A certificação da empresa segundo o referencial BRC versão 6 no prazo previsto não foi possível mas este projeto contribuiu para que que esta venha a ser bem-sucedida, tendo sido definida a base processual e documental necessária para o efeito.

Controle social e políticas públicas: contribuições para aprimorar o controle social do programa nacional de alimentação escolar

The National School Feeding Programme (PNAE) is a public policy in Brazil for over 60 years and represents one of the most important programs of feeding and nutrition in the world. The role of family farming as a source of employment in rural areas, food provider and for ensuring much of the Brazil’s food security is constantly present at the government's and social movement’s agendas. Law 11.947 of 2009 marked its integration in the food supply for the National School Feeding Programme. Article 14 of aforementioned law highlights that a minimum of 30% (thirty percent) of the funds transferred by the National Development Fund Education (FNDE) to the Programme must be used for the purchase of food directly from family farmers or their organizations. The national school feeding policy under the responsibility of the FNDE and is subjected to agencies of internal control, such as the General Controllership of the Union (CGU), of external control, such as the Audit Courts of the Union and the of the states, and to the social control of the school feeding councils. Those funds are transferred to the implementing agencies, which are the education offices of the states, municipalities and of the Federal District. These entities must annually present their accountings to the School Feeding Councils, which analyze them and then issue a conclusive report to the FNDE, approving with or without reservations, or rejecting them. In this sense, this research aims to propose parameters that should contribute to the improvement of the social control over purchases from family farming for the National School Feeding Programme. The study was conducted by non parametric sampling alongside the managers of the implementing entities, school feeding councils and Family Farming Organizations all across Brazil, from the databases provided by FNDE and by the National Union of Cooperatives of Family Agriculture and Solidarity Economy (Unicafes). The study points out that the legal framework of PNAE seeks to ensure the participation of family farming in the food supply for the Programme, despite allowing the executing agencies to justify the non-compliance of the minimum required in a number of ways. The survey also signalizes that the school feeding councils follow the implementation of the Programme very shyly, and points out that there is room to expand and enhance the participation of these councils and organizations of family farming in the execution of PNAE. Its effectiveness requires a constant and effective process of training of the agents involved in the Programme.

Caraterização in vitro dos efeitos tóxicos da patulina na integridade do epitélio intestinal e potenciais efeitos protetores da cisteína

A mucosa intestinal é a primeira barreira biológica encontrada pelas micotoxinas presentes nos alimentos, sendo a patulina, uma micotoxina produzida por fungos do género Penicillium spp., uma preocupação particular atendendo a que a exposição humana a esta micotoxina pode conduzir a efeitos imunológicos, neurológicos e gastrointestinais. Considerando estes efeitos para a saúde, o presente estudo tem como objetivos a avaliação do efeito tóxico da exposição intestinal a patulina, bem como a determinação do potencial efeito protetor da coadministração de patulina e cisteína na membrana intestinal, utilizando para o efeito células Caco-2. A integridade da membrana intestinal foi determinada pela medição da resistência elétrica transepitelial (TEER). Os resultados evidenciaram um decréscimo acentuado nos valores de TEER após 24 horas de exposição celular a 95 μM de patulina. Para as concentrações mais reduzidas verificou-se uma redução máxima inferior a 25% após 24 horas de exposição. A coadministração de patulina (95 μM) e cisteína (40 μM) revelou um decréscimo nos valores de TEER. O tratamento com cisteína em concentrações superiores ( 400 μM) revelou efeito protetor da membrana intestinal, tendo em conta os valores de TEER. Estes resultados contribuem para uma avaliação do risco mais precisa associada à exposição a contaminantes alimentares.