Condições de saúde, trabalho e hábitos de vida de trabalhadores de um campus universitário

Pós-graduação em Enfermagem (mestrado profissional) - FMB

A sedação sobre os potenciais evocados auditivos em cães

Brainstem auditory evoked potential (BAEP) reflects the electrical activity along the auditory pathway, from the cochlea to the brainstem, and contributes for the diagnosis of deafness in dogs. BAEP recording may require chemical restraint in some cases, so this study was designed to analyze the impact of sedation with morphine and acepromazine on the BAEP recordings of 16 dogs with normal hearing. BAEPs were recorded before and during sedation with a combination of morphine (0.5mgkg(-1)) and acepromazine (0.05mgkg(-1)) given intramuscularly. The protocol employed allowed safe and effective animal restraint. Sedation increased the latency of waves II and III and intervals I-III and I-V but did not interfere with wave identification. and showed to be safe in the dogs tested. Based on the current literature this is the first study which assessed the impact of sedation on BAEPs in dogs in Brazil.

Cardiovascular risk and mortality in end-stage renal disease patients undergoing dialysis: sleep study, pulmonary function, respiratory mechanics, upper airway collapsibility, autonomic nervous activity, depression, anxiety, stress and quality of life: a prospective, double blind, randomized controlled clinical trial

Background: Chronic kidney disease (CKD) is one of the most serious public health problems. The increasing prevalence of CKD in developed and developing countries has led to a global epidemic. The hypothesis proposed is that patients undergoing dialysis would experience a marked negative influence on physiological variables of sleep and autonomic nervous system activity, compromising quality of life.Methods/Design: A prospective, consecutive, double blind, randomized controlled clinical trial is proposed to address the effect of dialysis on sleep, pulmonary function, respiratory mechanics, upper airway collapsibility, autonomic nervous activity, depression, anxiety, stress and quality of life in patients with CKD. The measurement protocol will include body weight (kg); height (cm); body mass index calculated as weight/height(2); circumferences (cm) of the neck, waist, and hip; heart and respiratory rates; blood pressures; Mallampati index; tonsil index; heart rate variability; maximum ventilatory pressures; negative expiratory pressure test, and polysomnography (sleep study), as well as the administration of specific questionnaires addressing sleep apnea, excessive daytime sleepiness, depression, anxiety, stress, and quality of life.Discussion: CKD is a major public health problem worldwide, and its incidence has increased in part by the increased life expectancy and increasing number of cases of diabetes mellitus and hypertension. Sleep disorders are common in patients with renal insufficiency. Our hypothesis is that the weather weight gain due to volume overload observed during interdialytic period will influence the degree of collapsibility of the upper airway due to narrowing and predispose to upper airway occlusion during sleep, and to investigate the negative influences of haemodialysis in the physiological variables of sleep, and autonomic nervous system, and respiratory mechanics and thereby compromise the quality of life of patients.

Characterisation of the methylation pattern in the intragenic CpG island of the IGF2 gene in Bos taurus indicus cumulus cells during in vitro maturation

The aim of this study was to characterise the methylation pattern in a CpG island of the IGF2 gene in cumulus cells from 1-3 mm and a parts per thousand yenaEuro parts per thousand 8.0 mm follicles and to evaluate the effects of in vitro maturation on this pattern.Genomic DNA was treatment with sodium bisulphite. Nested PCR using bisulphite-treated DNA was performed, and DNA methylation patterns have been characterised.There were no differences in the methylation pattern among groups (P > 0.05). Cells of pre-IVM and post-IVM from small follicles showed methylation levels of 78.17 +/- 14.11 % and 82.93 +/- 5.86 %, respectively, and those from large follicles showed methylation levels of 81.81 +/- 10.40 % and 79.64 +/- 13.04 %, respectively. Evaluating only the effect of in vitro maturation, cells of pre-IVM and post-IVM COCs showed methylation levels of 80.17 +/- 12.01 % and 81.19 +/- 10.15 %.In conclusion, the methylation levels of the cumulus cells of all groups were higher than that expected from the imprinted pattern of somatic cells. As the cumulus cells from the pre-IVM follicles were not subjected to any in vitro manipulation, the hypermethylated pattern that was observed may be the actual physiological methylation pattern for this particular locus in these cells. Due the importance of DNA methylation in oogenesis, and to be a non-invasive method for determining oocyte quality, the identification of new epigenetic markers in cumulus cells has great potential to be used to support reproductive biotechniques in humans and other mammals.

Leukotoxicity of Aggregatibacter actinomycetemcomitans in generalized aggressive periodontitis in Brazilians and their family members

Objective: The purpose of this study was to examine the leukotoxin promoter types of Aggregatibacter actinomycetemcomitans clones in subjects with generalized aggressive periodontitis (GAgP) and in their family members (FM). Material and Methods: Thirty-five patients with GAgP (33.9+/-7.1 years), 33 of their FM (22.8+/-11.4 years), and 41 patients with chronic periodontitis (CP) (44.1+/-9.4 years) were clinically analyzed using the plaque index, gingival index, probing depth (PD), and clinical attachment level (CAL). Subgingival biofilm samples were collected from four interproximal periodontal sites (>PD and >CAL) of each patient. The presence of A. actinomycetemcomitans and its leukotoxic clone was confirmed by polymerase chain reaction (PCR). Results: A. actinomycetemcomitans was observed in 23 (51.1%) GAgP patients and 16 (30.1%) CP patients. Thirty-seven (94.8%) patients showed minimally leukotoxic strains and 2 (5.1%) showed highly leukotoxic strains. In the FM group, 10 (30.3%) had aggressive periodontitis (AgP), 12 (36.3%) had CP, 11 (33.3%) were periodontally healthy or had gingivitis, and 12.2% were A. actinomycetemcomitans positive. Greater full mouth PD and CAL were observed in GAgP patients positive for the bacteria than those negative for it (p<0.05), and the presence of A. actinomycetemcomitans positively correlated with GAgP (Odds ratio, 3.1; confidence interval, 1.4-7.0; p=0.009). Conclusions: The presence of A. actinomycetemcomitans was associated with the clinical condition of GAgP, with most patients exhibiting a generalized form of the disease and minimally leukotoxic clones. Most of the relatives of GAgP patients presented either CP or AgP.

Clinical Comparison of the Effectiveness of Single-file Reciprocating Systems and Rotary Systems for Removal of Endotoxins and Cultivable Bacteria from Primarily Infected Root Canals

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

A Catuama e o bilobalide na regeneração nervosa periférica de ratos submetidos à secção do nervo isquiático

The Catuama((R)) is composed of four Brazilian plants extracts (Paullinia cupana, Trichilia catigua, Ptychopetalum olacoides e Zingiber officinale). The Catuama((R)) is known as having neuroprotector, anti-inflammatory, antioxidant and antidepressant effects. Bilobalide, extracted from leaves of Ginkgo biloba, is known by its neuroprotective effect in the central and peripheral nervous systems. The present study evaluates the effect of Catuama((R)) and bilobalide on peripheral nerve regeneration in rats following a sciatic nerve section. Sciatic nerve of forty adult rats was transected with a 10-mm gap and the proximal and distal nerve stumps were fixed in a silicone tube filled with liquid collagen. The animals were divided into four groups: the control group (A), two groups treated with Catuama((R)) by gavage along 28 days after the surgery in different doses of 100 (B) and 400mg. kg(-1) (C) and the group using 200 mu M bilobalide (D) associated with the liquid collagen in the silicone tube. Evaluations were done by a walk test on the first, fifth and tenth week after the surgery. Electrophysiological stimulation and quantitative and qualitative histological analyses of the sciatic nerve and gastrocnemius muscle were also performed on the tenth week after the surgery. All groups showed good regeneration but no statistical difference was found between treatments and control groups (P > 0.05).

Leaf anatomical traits corroborate the leaf economic spectrum: a case study with deciduous forest tree species

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Estabelecendo uma amizade entre o homem e os anfíbios anuros: uma questão de educação na Escola do Meio Ambiente, Botucatu, SP

O presente estudo foi realizado na Escola do Meio Ambiente (EMA) (22°55'23”S e 48°27'28”W), com a parceria da Escola Estadual “Parque Residencial 24 de Maio”, ambas localizadas no município de Botucatu. O objetivo deste trabalho foi a elaboração de um material de apoio pedagógico. À aula de campo dada aos alunos da Escola Estadual, comprovando a importância dessas atividades no processo de sensibilização dos mesmos. Para investigar os conhecimentos prévios dos alunos do primeiro e segundo ano do Ensino Médio da Escola Estadual, escolhidos como público alvo do trabalho, foi aplicado um questionário. Esse contava com sete perguntas dissertativas sobre o tema anfíbio anuros. Após a análise desses resultados, foi elaborado o referido material de apoio. Um folder que recebeu o titulo de “Salve os anfíbios anuros! - preservar os anfíbios é ser humano”. Este material foi utilizado e distribuído na aula de campo elaborada para esse grupo de alunos. Na atividade de campo os alunos puderam ter contato com esses animais, perdendo medos e sanando dúvidas sobre os mesmos. Para finalizar, o questionário foi reaplicado aos alunos, comprovando-se assim, a relevância da atividade proposta a eles

Levantamento da anurofauna da Escola do Meio Ambiente, Botucatu-SP

O objetivo do presente estudo foi realizar o inventário da anurofauna, assim como investigar a distribuição espacial, temporal e a abundância relativa das espécies localizadas em um mosaico vegetacional na região de Botucatu, SP (22 º55’ 23’’S e 45 º27’ 28’’W). A área estudada situa-se na Escola do Meio Ambiente, possui aproximadamente 12 hectares e uma grande heterogeneidade de habitats. Os dados foram obtidos entre outubro de 2006 a outubro de 2007 e entre setembro de 2009 e setembro de 2010, totalizando 29 dias de campo. Os métodos utilizados foram busca ativa e armadilhas de interceptação e queda. Foram identificadas 26 espécies de anfíbios de cinco famílias: Bufonidae (2 sp), Hylidae (15), Leptodactylidae (6), Leiuperidae (2) e Microhylidae (1), distribuídas em três grandes áreas: área aberta, mata e borda de mata. Os ambientes de área aberta foram utilizados pela maioria dos anfíbios (n=21), enquanto a borda de mata apresentou a menor riqueza (n=5). Treze espécies foram observadas na mata. As espécies Physalaemus cuvieri, e Leptodacylus furnarius foram as mais generalistas, sendo encontradas em seis e cinco dos sete ambientes estudados, respectivamente. Entre as espécies estudadas, Leptodactylus bokermanni, foi a única registrada em apenas um dos ambientes (borda da mata). As espécies mais abundantes na comunidade foram Dendropsophus nanus representando 13%, seguida de Dendropsophus sanborni e Scinax fuscomarginatus ambas com 12 % dos indivíduos. As menos abundantes foram: Rhinella icterica, Leptodactylus mystacinus e Eupemphix nattereri onde cada uma contribuiu com 0,2% dos indivíduos registrados. A grande maioria das espécies foi registrada no período quente e chuvoso (setembro a março), com exceção da espécie Scinax hiemalis que esteve presente na época fria e seca. Já Hypsiboas ...(Resumo completo, clicar acesso eletrônico abaixo)

Estudo de caso: formulação de custo de um produto de usinagem em uma pequena empresa

Many variables are indirectly involved in the transformation of raw material into a metallurgical industry, such as machine hours, hours of hand labor directly and / or indirect, setup time, etc. This research focuses on the complexity of formulating the cost of metallurgical products, based on a case study, in which one has incurred a large loss on the sale of a product called Punch. The main objective of this work is to define the variables of the cost of members and other metallurgical products, so simplified general, checking what were the failures costing the case studied, in order to help others. The method of formulating cost was determined as recommended by SEBRAE guidance for small businesses. The results showed a loss R$ 13.201,00 in the batch of 15 units of punch. Possible improvements have been identified for reducing the production cost

A tradução dos olhos em Clarice Lispector: uma investigação baseada em corpus

This investigation has as an objective to observe the translation of the word “eyes” and its collocates, in similar and (re)used fragments extracted from two books written by Clarice Lispector, A Descoberta do Mundo, translated by Giovanni Pontiero as Discovering the World and Uma Aprendizagem ou o Livro dos Prazeres, translated by Richard A. Mazzara and Lorri A. Parris as An Apprenticeship or The Book of Delights.Another objective is to identify aspects of normalization found in the respective translations of these fragments. The metodology is situated in the field of Corpus-based Translation Studies, (proposed by Baker, 1993, 1995, 1996, 1999, 2004; studies on normalization by de Scott, 1998); research and project by Camargo 2003a, 2003b, 2004, 2008), and in Corpus Linguistics (studies by Berber Sardinha, 2004); also, it is based on the author’s critical heritage (studies by Gotlib, 1993, 2009; Nunes, B., 1995; Sant‘Anna, 1997; Ruggero 2000; Sá, O., 2000; Franco Júnior, 2000; Ranzolin (1985), Varin, 2002; e Cherem, 2003). The results found in this research enabled to carry out a comparative study among the respective translators concerning tendencies to normalization and show Pontiero’s smaller tendency in relation to the couple of translators Richard A. Mazzara e Lorri A. Parris.

Tratamento multidisciplinar: relato de casos clínicos

Introduction: Adult patients are more prone to periodontal disease mainly caused by poor plaque control. In these patients, orthodontic movement is not contraindicated, but it is necessary to evaluate their periodontal status so that we can establish the appropriate treatment plan. Objective: The objective of this article is to describe and discuss the clinical cases of severely periodontally compromised individuals in need of oral rehabilitation. Methods: The study consisted of orthodontic treatment of two cases with periodontal involvement. After clinical and radiographic examinations, the cases were analyzed by a multidisciplinary team of Orthodontics, Periodontics and Prosthodontics, in order to provide the patient with the best possible esthetic, functional and stability outcomes. Periodontal treatment consisted of supra and subgingival scaling prior to orthodontic treatment, and regular maintenance performed on a quarterly basis throughout orthodontic movement. Activation was carried out every 45 to 50 days, with light forces. Retention remains to the present day, even after completion of the rehabilitation. Conclusion: Multidisciplinary oral rehabilitation treatment yields satisfactory results. The interaction between Orthodontics and Periodontics reveals that patients with reduced, but healthy periodontium, can receive orthodontic treatment as long as the forces applied do not exceed patient's biological limits.

Chemical analysis and Vickers hardness of orthodontic mini-implants

Orthodontic mini-implants are used in clinical practice to provide efficient and aesthetically-pleasing anchorage. AIM: To evaluate the hardness Vickers hardness and chemical composition of mini-implant titanium alloys from five commercial brands. METHODS: Thirty self-drilling mini-implants, six each from the following commercial brands, were used: Neodent NEO, Morelli MOR, Sin SIN, Conexão CON, and Rocky Mountain RMO. The hardness and chemical composition of the titanium alloys were performed by the Vickers hardness test and energy dispersive X-ray spectroscopy, respectively. RESULTS: Vickers hardness was significantly higher in SIN implants than in NEO, MOR, and CON implants. Similarly, VH was significantly higher in RMO implants than in MOR and NEO ones. In addition, VH was higher in CON implants than in NEO ones. There were no significant differences in the proportions of titanium and aluminum in the mini-implant alloy of the five commercial brands. Conversely, the proportion of vanadium differed significantly between CON and MOR/NEO implants. CONCLUSIONS: Mini-implants of different brands presented distinct properties of hardness and composition of the alloy.

O poema em música e a música além do poema: estudo sobre a relação entre música e poesia em obras de Almeida Prado, Eduardo Guimarães Álvares e Harry Crowl

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)