Sapientia, 1954, Vol. IX, nº 34 (número completo)

Contenido: Actualidad de San Agustín / La Dirección – Dialecticidad de la naturaleza humana y su problemática esencial en el pensamiento de San Agustín / Michele F. Sciacca – Los grados de perfección del alma según San Agustín / Alberto Caturelli – Determinación de la influencia neoplatónica en la formación del pensamiento de San Agustín / Octavio N. Derisi – Fundamentos de la realidad en S. Agustín / Manuel Gonzalo Casas – Notas y comentarios -- Bibliografía

Projetos de lei ordinária apresentados na Câmara dos Deputados entre 1999 e 2006 : tramitação geral e dos relacionados à saúde

Análise da tramitação dos projetos de lei ordinária (PL) apresentados na Câmara dos Deputados (CD) entre 1999 e 2006 e também de um subgrupo de PL relacionados à saúde (PL-Saúde), apresentados no mesmo período. Foram elaborados: um banco de dados (a partir de planilhas do Centro de Informática da CD, contendo variáveis aferidas em 07 de março de 2007) e uma classificação temática para os PL-Saúde. Observou-se que: a) foram apresentados 15.246 PL na CD de 1999 a 2006 (com padrão temporal similar na 51ª e na 52ª Legislaturas); b) 2,8% dos PL foram convertidos em lei e 74,8% foram arquivados; c)96,7% dos PL eram de autoria do Legislativo, 7%, do Executivo e 0,6% de outros órgãos; d) dos 428 PL convertidos em lei, 53,5% eram de autoria do Legislativo e 38%, do Executivo; e) considerando os PL convertidos em lei, os do Executivo tramitaram com tempo médio 2,6 vezes mais curto que o da CD. A avaliação temática dos PL de 1999 a 2006 detectou 4.358 PL-Saúde (28,6% do total apresentado), dos quais 34,3% relacionam-se a ações de saúde; 30,9%, a redução de risco de agravos à saúde; 28,8%, a benefícios relacionados à saúde e 5,9%, a direitos relacionados à saúde. Os PL do Executivo convertidos em lei tramitaram com tempo médio 4 vezes mais curto que o da CD. A metodologia aplicada detectou padrões temporais diferenciados de apresentação de PL e de sua conversão em lei, conforme a autoria, como também demonstrou a viabilidade de estudos de monitoramento sistemático da tramitação de PL, inclusive no que se refere a temas.

O estudo do lobby no legislativo - o caso de sucesso da CDU-CD

Este estudo visa a expor o caso de sucesso da construção da legislação urbanística federal brasileira por meio do lobby e da participação política de entidades representativas na Comissão de Desenvolvimento Urbano da Câmara dos Deputados.

Basic equations for suspension flows with interphase mass-transfer

In the case of suspension flows, the rate of interphase momentum transfer M(k) and that of interphase energy transfer E(k), which were expressed as a sum of infinite discontinuities by Ishii, have been reduced to the sum of several terms which have concise physical significance. M(k) is composed of the following terms: (i) the momentum carried by the interphase mass transfer; (ii) the interphase drag force due to the relative motion between phases; (iii) the interphase force produced by the concentration gradient of the dispersed phase in a pressure field. And E(k) is composed of the following four terms, that is, the energy carried by the interphase mass transfer, the work produced by the interphase forces of the second and third parts above, and the heat transfer between phases. It is concluded from the results that (i) the term, (-alpha-k-nabla-p), which is related to the pressure gradient in the momentum equation, can be derived from the basic conservation laws without introducing the "shared-pressure presumption"; (ii) the mean velocity of the action point of the interphase drag is the mean velocity of the interface displacement, upsilonBAR-i. It is approximately equal to the mean velocity of the dispersed phase, upsilonBAR-d. Hence the work terms produced by the drag forces are f(dc) . upsilonBAR-d, and f(cd) . upsilonBAR-d, respectively, with upsilonBAR-i not being replaced by the mean velocity of the continuous phase, upsilonBAR-c; (iii) by analogy, the terms of the momentum transfer due to phase change are upsilonBAR-d-GAMMA-c, and upsilonBAR-d-GAMMA-d, respectively; (iv) since the transformation between explicit heat and latent heat occurs in the process of phase change, the algebraic sum of the heat transfer between phases is not equal to zero. Q(ic) and Q(id) are composed of the explicit heat and latent heat, so that the sum Q(ic) + Q(id)) is equal to zero.

TiC原位增强块状Cu_(47)Ti_(34)Zr_(11)Ni_8非晶合金复合材料

在电弧炉中利用吸铸法制备了直径1-4 mm的原位生成TiC和β-Ti枝晶联合增强的块状Cu47Ti34Zr11Ni8非晶合金复合材料.DSC热分析结果表明,原位生成TiC颗粒的引入,没有影响基体合金的非晶形成能力.用OM,XRD,SEM,EDS等方法研究了复合材料的相组成、微观组织以及成分分布,结果表明,TiC颗粒作为异质形核中心促进了β-Ti枝晶的形成,形成了TiC颗粒和β-Ti枝晶联合增强的块状Cu47Ti34Zr11Ni8非晶合金复合材料,而且β-Ti枝晶的尺寸和数量与TiC颗粒的多少以及试样的尺寸有关.室温压缩试验表明,同单相非晶合金相比,块状Cu47Ti34Zr11Ni8非晶合金复合材料提高了抗压强度及塑性.

Niveles de nutrientes y pigmentos fotosintéticos en el Río de la Plata interior (55°-59°W 34°-36°S)

The "Río de la Plata" River is one of the less studied systems of the basin with respect to its biological and ecological aspects. Twenty four and twenty seven surface stations were sample on September 22-23 and November 22-23, 1982, respectively. The section studied is part of the zone called inner "Río de la Plata" River. The discharge was 29.000 m3/s in September and 45.200 m3/s in November. Total phosphorus (PT), total organic nitrogen (NOT), chemical oxygen demad (COT) and total cholrophyll were measured. Dissolved oxygen (DO), turbidity (TURB), pH and electrical conductivity (K20) were also measured at the surface with a HORIBA U7 sensor on November 1982. PT was 72-208 mg/m3 and 66-205 mg/m3 in September and November repectively; the higher values were near the Argentinian coast and the outer zone. NOT was 33-106 µM and 49-117 µM and CHL was 1.4-5.8 mg/m3 and 1.3-9.4 mg/m3. TURB was between 44 and 240 NTU in November; the maximun value were obseved in the central zone. Steep K20 gradients were found near both coast. The reduced organic carbon load into the lower and external part of the "Río de la Plata" River was estimated. (Document contains 40 pages.)

Ecological condition of coastal ocean waters along the U.S. Western Continental Shelf: 2003

Executive Summary: The western National Coastal Assessment (NCA-West) program of EPA, in conjunction with the NOAA National Ocean Service (NOS), conducted an assessment of the status of ecological condition of soft sediment habitats and overlying waters along the western U.S. continental shelf, between the target depths of 30 and 120 m, during June 2003. NCA-West and NOAA/NOS partnered with the West Coast states (Washington (WA), Oregon (OR), and California (CA)), and the Southern California Coastal Water Research Project (SCCWRP) Bight ’03 program to conduct the survey. A total of 257 stations were sampled from Cape Flattery, WA to the Mexican border using standard methods and indicators applied in previous coastal NCA projects. A key study feature was the incorporation of a stratified-random sampling design with stations stratified by state and National Marine Sanctuary (NMS) status. Each of the three states was represented by at least 50 random stations. There also were a total of 84 random stations located within NOAA’s five NMSs along the West Coast including the Olympic Coast NMS (OCNMS), Cordell Bank NMS (CBNMS), Gulf of Farallones NMS (GFNMS), Monterey Bay NMS (MBNMS), and Channel Islands NMS (CINMS). Collection of flatfish via hook-and-line for fish-tissue contaminant analysis was successful at 50 EMAP/NCA-West stations. Through a collaboration developed with the FRAM Division of the Northwest Fisheries Science Center, fish from an additional 63 stations in the same region and depth range were also analyzed for fish-tissue contaminants. Bottom depth throughout the region ranged from 28 m to 125 m for most stations. Two slightly deeper stations from the Southern California Bight (SCB) (131, 134 m) were included in the data set. About 44% of the survey area had sediments composed of sands (< 20% silt-clay), about 47% was composed of intermediate muddy sands (20-80% silt-clay), and about 9% was composed of muds (> 80% silt-clay). The majority of the survey area (97%) had relatively low percent total organic carbon (TOC) levels of < 2%, while a small portion (< 1%) had high TOC levels (> 5%), in a range potentially harmful to benthic fauna. Salinity of surface waters for 92% of the survey area were > 31 psu, with most stations < 31 psu associated with the Columbia River plume. Bottom salinities ranged only between 31.6 and 34.4 psu. There was virtually no difference in mean bottom salinities among states or between NMS and non-NMS stations. Temperatures of surface water (range 8.5 -19.9 °C) and bottom water (range 5.8 -14.7 °C) averaged several degrees higher in CA in comparison to WA and OR. The Δσt index of watercolumn stratification indicated that about 31% of the survey area had strong vertical stratification of the water column. The index was greatest for waters off WA and lowest for CA waters. Only about 2.6 % of the survey area had surface dissolved oxygen (DO) concentrations ≤ 4.8 mg/L, and there were no values below the lower threshold (2.3 mg/L) considered harmful to the survival and growth of marine animals. Surface DO concentrations were higher in WA and OR waters than in CA, and higher in the OC NMS than in the CA sanctuaries. An estimated 94.3% of the area had bottom-water DO concentrations ≤ 4.8 mg/L and 6.6% had concentrations ≤ 2.3 mg/L. The high prevalence of DO from 2.3 to 4.8 mg/L (85% of survey area) is believed to be associated with the upwelling of naturally low DO water across the West Coast shelf. Mean TSS and transmissivity in surface waters (excluding OR due to sample problems) were slightly higher and lower, respectively, for stations in WA than for those in CA. There was little difference in mean TSS or transmissivity between NMS and non-NMS locations. Mean transmissivity in bottom waters, though higher in comparison to surface waters, showed little difference among geographic regions or between NMS and non-NMS locations. Concentrations of nitrate + nitrite, ammonium, total dissolved inorganic nitrogen (DIN) and orthophosphate (P) in surface waters tended to be highest in CA compared to WA and OR, and higher in the CA NMS stations compared to CA non-sanctuary stations. Measurements of silicate in surface waters were limited to WA and CA (exclusive of the SCB) and showed that concentrations were similar between the two states and approximately twice as high in CA sanctuaries compared to OCNMS or nonsanctuary locations in either state. The elevated nutrient concentrations observed at CA NMS stations are consistent with the presence of strong upwelling at these sites at the time of sampling. Approximately 93% of the area had DIN/P values ≤ 16, indicative of nitrogen limitation. Mean DIN/P ratios were similar among the three states, although the mean for the OCNMS was less than half that of the CA sanctuaries or nonsanctuary locations. Concentrations of chlorophyll a in surface waters ranged from 0 to 28 μg L-1, with 50% of the area having values < 3.9 μg L-1 and 10% having values > 14.5 μg L-1. The mean concentration of chlorophyll a for CA was less than half that of WA and OR locations, and concentrations were lowest in non-sanctuary sites in CA and highest at the OCNMS. Shelf sediments throughout the survey area were relatively uncontaminated with the exception of a group of stations within the SCB. Overall, about 99% of the total survey area was rated in good condition (<5 chemicals measured above corresponding effect range low (ERL) concentrations). Only the pesticides 4,4′-DDE and total DDT exceeded corresponding effect range-median (ERM) values, all at stations in CA near Los Angeles. Ten other contaminants including seven metals (As, Cd, Cr, Cu, Hg, Ag, Zn), 2-methylnaphthalene, low molecular weight PAHs, and total PCBs exceeded corresponding ERLs. The most prevalent in terms of area were chromium (31%), arsenic (8%), 2-methylnaphthalene (6%), cadmium (5%), and mercury (4%). The chromium contamination may be related to natural background sources common to the region. The 2-methylnaphthalene exceedances were conspicuously grouped around the CINMS. The mercury exceedances were all at non-sanctuary sites in CA, particularly in the Los Angeles area. Concentrations of cadmium in fish tissues exceeded the lower end of EPA’s non-cancer, human-health-risk range at nine of 50 EMAP/NCA-West and nine of 60 FRAM groundfish-survey stations, including a total of seven NMS stations in CA and two in the OCNMS. The human-health guidelines for all other contaminants were only exceeded for total PCBs at one station located in WA near the mouth of the Columbia River. Benthic species richness was relatively high in these offshore assemblages, ranging from 19 to 190 taxa per 0.1-m2 grab and averaging 79 taxa/grab. The high species richness was reflected over large areas of the shelf and was nearly three times greater than levels observed in estuarine samples along the West Coast (e.g NCA-West estuarine mean of 26 taxa/grab). Mean species richness was highest off CA (94 taxa/grab) and lower in OR and WA (55 and 56 taxa/grab, respectively). Mean species richness was very similar between sanctuary vs. non-sanctuary stations for both the CA and OR/WA regions. Mean diversity index H′ was highest in CA (5.36) and lowest in WA (4.27). There were no major differences in mean H′ between sanctuary vs. nonsanctuary stations for both the CA and OR/WA regions. A total of 1,482 taxa (1,108 to species) and 99,135 individuals were identified region-wide. Polychaetes, crustaceans and molluscs were the dominant taxa, both by percent abundance (59%, 17%, 12% respectively) and percent species (44%, 25%, 17%, respectively). There were no major differences in the percent composition of benthic communities among states or between NMSs and corresponding non-sanctuary sites. Densities averaged 3,788 m-2, about 30% of the average density for West Coast estuaries. Mean density of benthic fauna in the present offshore survey, averaged by state, was highest in CA (4,351 m-2) and lowest in OR (2,310 m-2). Mean densities were slightly higher at NMS stations vs. non-sanctuary stations for both the CA and OR/WA regions. The 10 most abundant taxa were the polychaetes Mediomastus spp., Magelona longicornis, Spiophanes berkeleyorum, Spiophanes bombyx, Spiophanes duplex, and Prionospio jubata; the bivalve Axinopsida serricata, the ophiuroid Amphiodia urtica, the decapod Pinnixa occidentalis, and the ostracod Euphilomedes carcharodonta. Mediomastus spp. and A. serricata were the two most abundant taxa overall. Although many of these taxa have broad geographic distributions throughout the region, the same species were not ranked among the 10 most abundant taxa consistently across states. The closest similarities among states were between OR and WA. At least half of the 10 most abundant taxa in NMSs were also dominant in corresponding nonsanctuary waters. Many of the abundant benthic species have wide latitudinal distributions along the West Coast shelf, with some species ranging from southern CA into the Gulf of Alaska or even the Aleutians. Of the 39 taxa on the list of 50 most abundant taxa that could be identified to species level, 85% have been reported at least once from estuaries of CA, OR, or WA exclusive of Puget Sound. Such broad latitudinal and estuarine distributions are suggestive of wide habitat tolerances. Thirteen (1.2%) of the 1,108 identified species are nonindigenous, with another 121 species classified as cryptogenic (of uncertain origin), and 208 species unclassified with respect to potential invasiveness. Despite uncertainties of classification, the number and densities of nonindigenous species appear to be much lower on the shelf than in the estuarine ecosystems of the Pacific Coast. Spionid polychaetes and the ampharetid polychaete Anobothrus gracilis were a major component of the nonindigenous species collected on the shelf. NOAA’s five NMSs along the West Coast of the U.S. appeared to be in good ecological condition, based on the measured indicators, with no evidence of major anthropogenic impacts or unusual environmental qualities compared to nearby nonsanctuary waters. Benthic communities in sanctuaries resembled those in corresponding non-sanctuary waters, with similarly high levels of species richness and diversity and low incidence of nonindigenous species. Most oceanographic features were also similar between sanctuary and non-sanctuary locations. Exceptions (e.g., higher concentrations of some nutrients in sanctuaries along the CA coast) appeared to be attributable to natural upwelling events in the area at the time of sampling. In addition, sediments within the sanctuaries were relatively uncontaminated, with none of the samples having any measured chemical in excess of ERM values. The ERL value for chromium was exceeded in sediments at the OCNMS, but at a much lower percentage of stations (four of 30) compared to WA and OR non-sanctuary areas (31 of 70 stations). ERL values were exceeded for arsenic, cadmium, chromium, 2- methylnaphthalene, low molecular weight PAHs, total DDT, and 4,4′-DDE at multiple sites within the CINMS. However, cases where total DDT, 4,4′-DDE, and chromium exceeded the ERL values were notably less prevalent at CINMS than in non-sanctuary waters of CA. In contrast, 2-methylnaphthalene above the ERL was much more prevalent in sediments at the CINMS compared to non-sanctuary waters off the coast of CA. While there are natural background sources of PAHs from oil seeps throughout the SCB, this does not explain the higher incidence of 2-methylnaphthalene contamination around CINMS. Two stations in CINMS also had levels of TOC (> 5%) potentially harmful to benthic fauna, though none of these sites exhibited symptoms of impaired benthic condition. This study showed no major evidence of extensive biological impacts linked to measured stressors. There were only two stations, both in CA, where low numbers of benthic species, diversity, or total faunal abundance co-occurred with high sediment contamination or low DO in bottom water. Such general lack of concordance suggests that these offshore waters are currently in good condition, with the lower-end values of the various biological attributes representing parts of a normal reference range controlled by natural factors. Results of multiple linear regression, performed using full model procedures to test for effects of combined abiotic environmental factors, suggested that latitude and depth had significant influences on benthic variables regionwide. Latitude had a significant inverse influence on all three of the above benthic variables, i.e. with values increasing as latitude decreased (p< 0.01), while depth had a significant direct influence on diversity (p < 0.001) and inverse effect on density (p <0.01). None of these variables varied significantly in relation to sediment % fines (at p< 0.1), although in general there was a tendency for muddier sediments (higher % fines) to have lower species richness and diversity and higher densities than coarser sediments. Alternatively, it is possible that for some of these sites the lower values of benthic variables reflect symptoms of disturbance induced by other unmeasured stressors. The indicators in this study included measures of stressors (e.g., chemical contaminants, eutrophication) that are often associated with adverse biological impacts in shallower estuarine and inland ecosystems. However, there may be other sources of humaninduced stress in these offshore systems (e.g., bottom trawling) that pose greater risks to ambient living resources and which have not been captured. Future monitoring efforts in these offshore areas should include indicators of such alternative sources of disturbance. (137pp.) (PDF contains 167 pages)

DNA Damage and Transcriptional Changes in the Gills of Mytilus galloprovincialis Exposed to Nanomolar Doses of Combined Metal Salts (Cd, Cu, Hg)

[ENG]Aiming at an integrated and mechanistic view of the early biological effects of selected metals in the marine sentinel organism Mytilus galloprovincialis, we exposed mussels for 48 hours to 50, 100 and 200 nM solutions of equimolar Cd, Cu and Hg salts and measured cytological and molecular biomarkers in parallel. Focusing on the mussel gills, first target of toxic water contaminants and actively proliferating tissue, we detected significant dose-related increases of cells with micronuclei and other nuclear abnormalities in the treated mussels, with differences in the bioconcentration of the three metals determined in the mussel flesh by atomic absorption spectrometry. Gene expression profiles, determined in the same individual gills in parallel, revealed some transcriptional changes at the 50 nM dose, and substantial increases of differentially expressed genes at the 100 and 200 nM doses, with roughly similar amounts of up- and down-regulated genes. The functional annotation of gill transcripts with consistent expression trends and significantly altered at least in one dose point disclosed the complexity of the induced cell response. The most evident transcriptional changes concerned protein synthesis and turnover, ion homeostasis, cell cycle regulation and apoptosis, and intracellular trafficking (transcript sequences denoting heat shock proteins, metal binding thioneins, sequestosome 1 and proteasome subunits, and GADD45 exemplify up-regulated genes while transcript sequences denoting actin, tubulins and the apoptosis inhibitor 1 exemplify down-regulated genes). Overall, nanomolar doses of co-occurring free metal ions have induced significant structural and functional changes in the mussel gills: the intensity of response to the stimulus measured in laboratory supports the additional validation of molecular markers of metal exposure to be used in Mussel Watch programs

Manifestação solicitada pelo TCU à CONOF/CD acerca de relatório preliminar, relativo à consulta sobre a aplicação da Lei Complementar nº 141, de 2012, formulada pela Comissão de Seguridade Social e Família/CD : TC 046.061/2012‐6

Avalia o relatório elaborado pelo TCU acerca da consulta formulada pela CSSF/CD(TC 046.061/2012‐6). Procura a pacificação de entendimento no âmbito do Congresso Nacional.

Evaluation of the body burdens of heavy metals in five commercially important fishes from different locations in Kainji Lake

The level and distribution of some heavy metals viz Cadmium, Lead, Copper Zinc, and Cobalt in five commercially important fishes, water and sediments at three different locations in Kainj Lake were determined using standard methods. The results show that the ranges of heavy metals mu g/g in fishes in Dam site Laotian are: Cd (0.05~c0.01-20~c01), (Pb(ND-1.12 plus or minus )1), Cu (0.81~c25-2.93~c06), Zn (20.89 arrow right .15-36.78~c2.97), Co(0.08~c01-0.27~c02); in cover Dam, the ranges are Cd (0.04~c02-0.16~c0.2), Pb (nd-02~c01), Cu(0.75~c05-2.61~c13), Zn(15.70~c1.55-32.23~c2.70), Co(0.04~c02-0.25~c0.01) and in Yuna they are Cd (0.05~c01-0.14~c02), Pb (nd-0.32~c01), Cu (0.23~c07-2.70~c05), Zn(15.50 plus or minus `.35-25.62~c2.47), Co(0.07~c02-23~c0.01). The metals concentration (mg/l) in the water sample from Dam site, cover dam and Yuna respectively are Cd(0.007~c001,. 004~c001 and 0.005~001), Pb(013~c001, ND and ND), Cu(.055~c008.030~c007, 05 plus or minus .010), Zn(0.13~c01, 0.060 plus or minus .0055) and Co (.026 plus or minus .022 plus or minus .004, .024 plus or minus .004), while the metals concentration ( mu g/g) in sediments sample from Dam site, cover dam and Yuna are respectively Cd(.05 plus or minus .01, .02 plus or minus .01), Pb(16.00~c1.00, ND and 9.33~c1.01), Cu(24.00~c1.34, 4.26 plus or minus .91 and 11.08~c1.32), Zn(42.00~c1.00, 35~c10 and 38.00 plus or minus .45), Co(15.00~c1.17, 8.69~c1.21 and 10.91~c44). The concentrations of the tested heavy metals are within the acceptable standards of WHO (1987a)

Methods of feeding of some macrothricids (Cladocera, Macrothricidae). [Translation from: Informatsionnyi Byulleten Biologiya Vnutrennikh Vod 16, 29-34, 1973.]

The feeding methods of macrothricids was investigated in three species — Ophryoxus gracilis G.O. Sars, Ilyocryptus sordidus (Lievin) and Lathonura rectirostris O.F.Muller. The procuring of food in those species follows two methods: filtration of dispersed particles or scraping off and collecting food from the surface of the substrate. When collecting food by scraping off is the sole method of feeding (as in Lathonura rectirostris), movement along the substrate and feeding are combined so intimately that they appear as parts of one and the same mechanism.

Some abundant Tendipedid species (Diptera, Tendipedidae) of the Amu-Darya drainage basin. Acalcarella shilova, gen.n. only [Translation from: Ent.Obozr. 34 313-322, 1955]

This document is a partial translation of taxonomic notes on two Diptera species (Acalcarella Shilova, Acalcarrella nucus) which were abundant in a survey of Tendipedid species (Diptera, Tendipedidae) of the Amu Darya drainage basin. These taxonomic notes of the larva, pupa and adults include drawings of the Acalcarella nucus larva.

CICLOADICIONES [3+2] Y [8+2] Y SU APLICACIÓN A LA SÍNTESIS DE MOLÉCULAS MARCADAS Y SENSORES DE ADN FLUORESCENTES

La presente Tesis Doctoral está encaminada hacia el estudio de las cicloadiciones y su aplicación a la síntesis de moléculas marcadas y sensores de ADN fluorescentes. La primera línea de trabajo se basará en el diseño y síntesis de dos nuevos grupos de moléculas fluorescentes basadas en las benzo[a]imidazo[5,1,2-cd]indolizinas y en las 2,3,9c-triazociclo-penta[j,k]fluorenos mediante la cicloadición [8 + 2]. También se lleva a cabo la síntesis de los derivados los aductos [8+2] que pudieran interaccionar con el ADN y posteriores experimentos de unión al ADN de los compuestos sintetizados. Por otro lado, la segunda línea de investigación consistirá en la búsqueda de estrategias sintéticas para la obtención de un precursor de un inhibidor de HDAC para su utilización en estudios de biodistribución mediante técnicas basados en radioactividad, como son la de tomografía por emisión de positrones y el método de disección.