孤雌生殖累积世代数和雌体年龄对萼花臂尾轮虫繁殖的影响

采用单个体培养方法,研究了孤雌生殖的累积世代数和雌体年龄对萼花臂尾轮虫混交雌体形成和产卵量的影响.结果表明,随着轮虫孤雌生殖累积世代数的增加,各代中的总混交雌体百分率呈减小的趋势;年幼的雌体可产生较多的混交雌体;非混交雌体所产后代中的总混交雌体百分率具有随其祖母年龄的增大而增大的趋势.孤雌生殖的累积世代数对轮虫非混交雌体的平均产卵量无显著的影响,非混交雌体的年龄对其后代的平均产卵量亦无显著的影响.

乌鳢规模化繁育技术

20 0 0年 6月催产 5 0组成熟亲鱼 ,网箱静水孵化。共产卵 89 5万粒 ,受精率 90 % ,孵化率 98% ,培育成 3～ 4cm的鱼种 48 3万尾。总投入 19480元 ,产出 12 435万元 ,投入产出比为 1∶6 38。

铜、汞、镉、六六六和对硫磷对食蚊鱼生长的影响

对2日龄的食蚊鱼仔鱼的急性毒性,96小时LC_(50)是:铜,0.28毫克/升;汞,0.58亳克/升;镉,10.2毫克/升;六六六(丙体),0.53毫克/升;对硫磷,0.3毫克/升。依据20天的生长数据求得的铜、镉和对硫磷的最大允许毒物浓度分别是0.015—0.030、0.005—0.010和0.0015—0.0030毫克/升,相应的应用系数分别为0.05—0.1、0.0005—0.0009和0.005—0.01。

Cellular contractility and substrate elasticity: A numerical investigation of the actin cytoskeleton and cell adhesion

Numerous experimental studies have established that cells can sense the stiffness of underlying substrates and have quantified the effect of substrate stiffness on stress fibre formation, focal adhesion area, cell traction, and cell shape. In order to capture such behaviour, the current study couples a mixed mode thermodynamic and mechanical framework that predicts focal adhesion formation and growth with a material model that predicts stress fibre formation, contractility, and dissociation in a fully 3D implementation. Simulations reveal that SF contractility plays a critical role in the substrate-dependent response of cells. Compliant substrates do not provide sufficient tension for stress fibre persistence, causing dissociation of stress fibres and lower focal adhesion formation. In contrast, cells on stiffer substrates are predicted to contain large amounts of dominant stress fibres. Different levels of cellular contractility representative of different cell phenotypes are found to alter the range of substrate stiffness that cause the most significant changes in stress fibre and focal adhesion formation. Furthermore, stress fibre and focal adhesion formation evolve as a cell spreads on a substrate and leading to the formation of bands of fibres leading from the cell periphery over the nucleus. Inhibiting the formation of FAs during cell spreading is found to limit stress fibre formation. The predictions of this mutually dependent material-interface framework are strongly supported by experimental observations of cells adhered to elastic substrates and offer insight into the inter-dependent biomechanical processes regulating stress fibre and focal adhesion formation. © 2013 Springer-Verlag Berlin Heidelberg.

Amplified fragment length polymorphism analysis to identify the genetic structure of the Gymnocypris przewalskii (Kessler, 1876) population from the Qinghai Basin, China

Amplified fragment length polymorphism (AFLP) was used to analyse the genetic structure of 45 individuals of Gymnocypris przewalskii (Kessler, 1876), an endangered and state-protected rare fish species, from three areas [the Heima (HM), Buha (BH) and Shaliu rivers (SL), all draining into Qinghai Lake]. A total of 563 polymorphic loci were detected. The HM, BH and SL populations have 435, 433 and 391 loci, respectively (Zhu and Wu, 1975), which account for 77.26%, 76.91% and 69.45% of the total number of polymorphic loci of each population, respectively. The Nei indices of genetic diversities (H) of the three populations were calculated to be 0.2869 (HM), 0.2884 (BH) and 0.2663 (SL), respectively. Their Shannon informative indices are 0.4244, 0.4251 and 0.3915, respectively. Research results show that the mean genetic distance between HM and BH is the smallest (0.0511), between BH and SL is the second shortest (0.0608), and between HM and SL is the largest (0.0713), with the mean genetic distance among the three populations being over 0.05. Data mentioned above indicate that the three populations have a certain genetic differentiation. The total genetic diversity (H-t = 0.3045) and the mean value of genetic diversity within the population (H-s = 0.2786) indicate that the variations have mainly come from within the population.

Differential screening and characterization analysis of the egg envelope glycoprotein ZP3 cDNAs between gynogenetic and gonochoristic crucian carp

Gynogenetic silver crucian carp, Carassius auratus gibelio, is an intriguing model. system. In the present work, a systemic study has been initiated by introducing suppression subtractive hybridization technique into this model system to identify the differentially expressed genes in oocytes between gynogenetic silver crucian carp and its closely related gonochoristic color crucian carp. Five differential cDNA fragments were identified from the preliminary screening, and two of them are ZP3 homologues. Moreover, the full length ZP3 cDNAs were cloned from their oocyte cDNA libraries. The length of ZP3 cDNAs were 1378 bp for gyno-carp and 1367 bp for gono-carp, and they can be translated into proteins with 435 amino acids. Obvious differences are not only in the composition of amino acids, but also in the number of potential O-linked oligosaccharide sites. In addition, gyno-carp ZP3 amino acid sequence has an unexpected higher identity value with common carp (83.5%) than that with the closely related gono-carp (74.7%). The unique homology may be originated from the ancient hybridization. Northern blot analysis confirmed that expression of the ZP3 gene occurred exclusively in the oocytes. Because O-linked oligosaccharides on ZP3 have been demonstrated to play very important roles in fertilization, it is suggested that the extra O-linked glycosylation sites may be related to the unique sperm-egg recognition mechanism in gynogenesis.

HYDROGRAPHIC VARIATIONS IN SIZE STRUCTURE OF PLANKTON BIOMASS IN A CHANGJIANG FLOODPLAIN LAKE

The size structure of the planktonic community in a Changjiang floodplain lake (Lake Chenhu, Hubei, P. R. China) was described for the inundation period of May through September 1983. The modality of the Sheldon-type size distributions changed hydrographically with the spectral profiles being bimodal during low, rising, mid-high and falling water phases, and trimodal soon after filling and shortly before falling. The modal peaks corresponded respectively to the dominant organisms of chlorophytes and nauplii, while the troughs centered on the bacteria and macrocrustacean size classes in the lake. The slope of the normalized biomass spectrum (an index of plankton size distribution) was less than -1.0 for the filling and falling phases or close to -1.0 for the high water period, indicating that the planktonic biomass tended to decrease or evenly distributes across logarithmically ordered size classes, respectively. This observed variation in the size distribution of the plankton community mainly resulted from changes in water levels and contents of particulate inorganic matter (PIM) in the lake.

Interfaces in heterostructures of AlInGaN/GaN/Al2O3

Rutherford backscattering/channeling (RBS/C) and X-ray diffraction (XRD) are used to comprehensively characterize a heterostructure of AlInGaN/GaN/Al2O3(0001). The AlInGaN quaternary layer was revealed to process a high crystalline quality with a minimum yield of 1.4% from RBS/C measurements. The channeling spectrum of (1 (2) under bar 13) exhibits higher dechanneling than that of (0001) at the interface of AlInGaN/GaN. XRD measurements prove a coherent growth of AlInGaN on the GaN template layer. Combining RBS/C and XRD measurements, we found that the interface of GaN/Al2O3 is a nucleation layer, composed of a large amount of disorders and cubic GaN slabs, while the interface of AlInGaN/GaN is free of extra disordering (i.e. compare with the GaN layer). The conclusion is further evidenced by transmission electron microscopy (TEM). (c) 2005 Elsevier Ltd. All rights reserved.

黄土干旱区嫁接与地面覆盖对温室黄瓜的增产效应

在日光温室条件下,研究了嫁接与地面覆盖对黄瓜产量、耗水量及水分利用效率的影响。结果表明:(1)嫁接与地面覆盖均可提高黄瓜产量,但嫁接的增产幅度远小于地面覆盖处理;秸秆+地膜覆盖对增加早期产量和总产量均有良好效果,而地膜覆盖对早期产量的形成具有明显的促进作用,而后期产量明显低于秸秆覆盖处理。(2)嫁接与地面覆盖耦合的增产幅度均大于二者各自增产贡献之和,其中以嫁接与秸秆+地膜覆盖的耦合增产效果最佳,地膜覆盖与秸秆覆盖次之。(3)嫁接黄瓜的耗水量高于未嫁接与各地面覆盖处理,其中以地膜覆盖最节水,秸秆+地膜覆盖与秸秆覆盖次之。嫁接与地面覆盖均能提高水分利用率,其中以秸秆+地膜覆盖最高,嫁接最低。嫁接与各地面覆盖对水分利用效率的协同效应均大于加和效应,其中以嫁接与秸秆+地膜覆盖的协同效应最佳。

小麦/玉米苗期磷累积量对介质供磷水平反应的差异

小麦和玉米苗期是磷素营养的关键期和敏感期,研究两种作物苗期对介质供磷反应,可为合理施用磷肥提供参考。试验设缺磷对照、低磷胁迫、中等磷胁迫和正常供磷(P_2O_5含量分别为0、0.05mmol·L~(-1)、0.3mmol·L~(-1)和0.5mmol·L~(-1))4种磷水平,选取小麦"小偃22号"、"兰考4号"和玉米"屯玉65号"、"户单4号"为指标作物,用营养液培养法研究小麦、玉米苗期磷累积量对介质不同供磷水平的反应差异。结果表明,不同介质供磷水平下,两种作物苗期磷累积量显著不同且因作物类型、基因型、器官及测定时期不同而异。总体而言,介质供磷后,苗期早期生长阶段(出苗后25d以前),小麦的介质最佳供磷水平较玉米高;苗期后期(出苗后40～50d),小麦和玉米最佳供磷水平一致。如果以低磷胁迫作为对比进行分析,玉米苗期整株磷累积量对介质供磷的敏感性比小麦强;从不同基因型来看:"兰考4号"对介质供磷的敏感性强于"小偃22号","屯玉65号"和"户单4号"基本一致。缺磷条件下小麦较玉米磷效率高,供磷条件下玉米较小麦高;但不同基因型间规律性较差。

GaAs基上的InAs量子环制备

在分子束外延系统中,利用3 nm GaAs薄盖层将InAs自组装量子点部分覆盖,然后在500℃以及As_2气氛中退火一分钟,制成纳米尺度的InAs量子环.这一形成敏感地依赖于退火时的生长条件和生长InAs自组装量子点时的淀积量.InAs在GaAs表面的扩散以及同时发生的In-Ga互混控制着InAs量子环的形成.

光子晶体对nc-Ge/Si岛发光增强的模拟

在Si基集成光电子学的发展中,高效的Si基光源是人们不懈追求的目标.但是Si材料的间接带隙特性导致其发光效率低,更谈不上受激发射.于是人们探索了多种Si基材料体系来提高Si材料的发光效率,并在不同程度上取得了重要的进展.在众多的Si基发光材料体系中,Ge/Si量子点材料,不仅生长工艺与标准的CMOS工艺有很好的兼容性,而且发光波长能够覆盖重要的光通信波段即1.3～1.55 μm,因此成为实现Si基发光器件的重要途径之一.但是目前这种材料的发光效率仍很低,所以提高其发光效率自然成为人们关注的焦点.如果将光子晶体引入到nc-Ge/Si材料中,它不仅可以改变材料本身的自发发射特性,而且可以改变发射的光子的提取效率,从而使材料的发光效率得到增强.提出了在Ge/Si量子点材料中引入光子晶体结构来提高其发光效率,包括光子晶体点缺陷腔结构和带边模式工作的完整光子晶体结构,并从理论上分析了发光效率提高的原理.针对发光波长在1.5 μm附近的材料结构,模拟出了相应的光子晶体的结构参数.从模拟结果可以看出,对于缺陷腔的光子晶体结构,采用单点缺陷微腔很好地实现了单模运作,但是微腔内有源材料的体积很小,因此得到的发光效率很低.而采用耦合缺陷腔的结构和H2腔都增加了腔内有源区的体积.但是耦合腔与H2腔相比,谐振腔模减少,主谐振模式的峰值强度增加,更容易实现单模发光.因而更适用于提高nc-Ge/Si的发光效率.而带边模式工作的光子晶体结构,尺寸较大,不需引入缺陷,工艺上更容易实现.

非对称全内反射型SOI波导开关器件模型分析

根据有效折射率法和光束传播法分析了大截面单模SOI脊形X型交叉波导的传输特性.指出交叉角在1.5-2°内,因导波模式引起耦合作用而导致的串音小于-25dB;采用波动光学原理分析了非对称全内反射开关导模的传输和反射特性;讨论了等离子体色散效应,pn结大注入效应以及Goos-Hanchken位移,并分析了非对我全内反射型SOI光波导开关的电学性质.据此优化设计了该器件的结构参数和电学参数.