Toxic effects of brake wear particles on epithelial lung cells in vitro

ABSTRACT: BACKGROUND: Fine particulate matter originating from traffic correlates with increased morbidity and mortality. An important source of traffic particles is brake wear of cars which contributes up to 20% of the total traffic emissions. The aim of this study was to evaluate potential toxicological effects of human epithelial lung cells exposed to freshly generated brake wear particles. RESULTS: An exposure box was mounted around a car's braking system. Lung cells cultured at the air-liquid interface were then exposed to particles emitted from two typical braking behaviours ("full stop" and "normal deceleration"). The particle size distribution as well as the brake emission components like metals and carbons was measured on-line, and the particles deposited on grids for transmission electron microscopy were counted. The tight junction arrangement was observed by laser scanning microscopy. Cellular responses were assessed by measurement of lactate dehydrogenase (cytotoxicity), by investigating the production of reactive oxidative species and the release of the pro-inflammatory mediator interleukin-8. The tight junction protein occludin density decreased significantly (p < 0.05) with increasing concentrations of metals on the particles (iron, copper and manganese, which were all strongly correlated with each other). Occludin was also negatively correlated with the intensity of reactive oxidative species. The concentrations of interleukin-8 were significantly correlated with increasing organic carbon concentrations. No correlation was observed between occludin and interleukin-8, nor between reactive oxidative species and interleukin-8. CONCLUSION: These findings suggest that the metals on brake wear particles damage tight junctions with a mechanism involving oxidative stress. Brake wear particles also increase pro-inflammatory responses. However, this might be due to another mechanism than via oxidative stress.

Finite Element Analysis of Thermal Buckling in Auotmotive Clutch and Brake Discs

Thermal buckling behavior of automotive clutch and brake discs is studied by making the use of finite element method. It is found that the temperature distribution along the radius and the thickness affects the critical buckling load considerably. The results indicate that a monotonic temperature profile leads to a coning mode with the highest temperature located at the inner radius. Whereas a temperature profile with the maximum temperature located in the middle leads to a dominant non-axisymmetric buckling mode, which results in a much higher buckling temperature. A periodic variation of temperature cannot lead to buckling. The temperature along the thickness can be simplified by the mean temperature method in the single material model. The thermal buckling analysis of friction discs with friction material layer, cone angle geometry and fixed teeth boundary conditions are also studied in detail. The angular geometry and the fixed teeth can improve the buckling temperature significantly. Young’s Modulus has no effect when single material is applied in the free or restricted conditions. Several equations are derived to validate the result. Young’s modulus ratio is a useful factor when the clutch has several material layers. The research findings from this paper are useful for automotive clutch and brake discs design against structural instability induced by thermal buckling.

Development of transit coach bonded brake lining test equipment and test procedures. Progress report. Final report.

Urban Mass Transportation Administration, Washington, D.C.

Evaluation of hydraulic brake system tests of used 1973 and 1978 vehicles; Standard 105-75 - Hydraulic Brake Systems, Passenger Cars.

Mode of access: Internet.

Implementation analysis of brake inspectability requirements. Final report.

National Highway Traffic Safety Administration, Washington, D.C.

Cost and leadtime estimates for improved pillar padding, automatic slack adjusters, visual brake adjustment indicators and tractor/trailer ABS connections. Final report.

National Highway Traffic Safety Administration, Washington, D.C.

Evaluation of Hunter heavy duty plate brake tester. Interim final report.

National Highway Traffic Safety Administration, Washington, D.C.

NHTSA's Heavy Duty Vehicle Brake Research Program - report Number 10: evaluation of trailer antilock braking systems electrical powering. Interim final report.

National Highway Traffic Safety Administration, Washington, D.C.

The effect of brake adjustment on braking performance. Final report.

Mode of access: Internet.

Light vehicle antilock brake research program. Report 1 - evaluation of an electronic four sensor, three circuit integrated system. Final report.

Mode of access: Internet.

Light vehicle antilock brake research program. Report 2 - evaluation of a fully mechanical, two circuit, two sensor system. Final report.

Mode of access: Internet.

The development of a classification scheme for brake linings. Volume I. Summary report.

National Highway Traffic Safety Administration, Washington, D.C.

The development of a classification scheme for brake linings. Volume II. Final report.

National Highway Traffic Safety Administration, Washington, D.C.

State inspection program evaluation and data analysis: data base E SPSS frequencies tabulations for brake components and explanatory variables. Volume III. Final report.

National Highway Traffic Safety Administration, Office of Vehicle Safety Research, Washington, D.C.