Community mix, affordable housing and metropolitan planning strategy in Melbourne

Income segregation across Melbourne’s residential communities is widening, and at a pace faster than in some other Australian cities. The widening gap between Melbourne’s rich and poor communities raises fears about concentrations of poverty and social exclusion, particularly if the geography of these communities is such that they and their residents are increasingly isolated from urban services and employment centres. Social exclusion in our metropolitan areas and the government responses to it are commonly thought to be the proper domain of social and economic policy. The role of urban planning is typically neglected, yet it helps shape the economic opportunities available to communities in its attempts to influence the geographical location of urban services, infrastructure and jobs. Under the current metropolitan strategy ‘Melbourne 2030’ urban services and transport infrastructure are to be concentrated within Principal Activity Centres spread throughout the metropolitan area and it is the intention that lower-income households should have ready access to these activity centres. However, the Victorian state government has few housing policy instruments to achieve this goal and there are fears that community mix may suffer as house prices and rents are bid up in the vicinity of Principal Activity Centres, and lower-income households are displaced. But are these fears justified by the changing geography of house prices in the metropolitan region? This is the key research question addressed in this paper which examines whether the Victorian practice of placing reliance on the market to deliver affordable housing, while intervening to promote a more compact pattern of urban settlement, is effective.

Urban Growth Boundaries and their impact on land prices

Investigation of the effects of Urban Growth Boundaries (UGB) on land prices are restricted by a lack of good land market data. However, undeveloped land transactions at the urban fringe of the Melbourne metropolitan area in Australia are recorded in a data set that enables exploration of the impact of its UGB. Estimation can take account of endogeneity issues, while controlling for policy anticipation effects and other potential influences on land prices. OLS and instrumental variable estimates indicate that land prices rose substantially inside the UGB after its enactment in 2003 but did not rise much outside of it.

Electrochemical genosensors for the detection of Bonamia parasite. Selection of single strand-DNA (ssDNA) probes by simulation of the secondary structure folding

A post-PCR nucleic acid work by comparing experimental data, from electrochemical genosensors, and bioinformatics data, derived from the simulation of the secondary structure folding and prediction of hybridisation reaction, was carried out in order to rationalize the selection of ssDNA probes for the detection of two Bonamia species, B. exitiosa and B. ostreae, parasites of Ostrea edulis.Six ssDNA probes (from 11 to 25 bases in length, 2 thiolated and 4 biotinylated) were selected within different regions of B. ostreae and B. exitiosa PCR amplicons (300 and 304 bases, respectively) with the aim to discriminate between these parasite species. ssDNA amplicons and probes were analyzed separately using the "Mfold Web Server" simulating the secondary structure folding behaviour. The hybridisation of amplicon-probe was predicted by means of "Dinamelt Web Server". The results were evaluated considering the number of hydrogen bonds broken and formed in the simulated folding and hybridisation process, variance in gaps for each sequence and number of available bases. In the experimental part, thermally denatured PCR products were captured at the sensor interface via sandwich hybridisation with surface-tethered probes (thiolated probes) and biotinylated signalling probes. A convergence between analytical signals and simulated results was observed, indicating the possibility to use bioinformatic data for ssDNA probes selection to be incorporated in genosensors. (C) 2011 Elsevier B.V. All rights reserved.

Educação, inclusão e políticas públicas: a educação especial no município de Marabá-Pará

Este trabalho investigou como se deu a implementação de Políticas Públicas para Educação Especial no município de Marabá-Pará. Para tanto se fez necessário analisar e refletir o papel da Secretaria Municipal de Educação na efetivação dessas políticas, tendo como focos a política de inclusão escolar e o processo de municipalização do Ensino Fundamental. A temática foi desenvolvida por meio de uma pesquisa qualitativa sobre o processo de inclusão escolar, pois a última década, em nosso país, marca o início da luta da sociedade civil para que se façam valer os direitos preconizados pela Constituição Federal de 1988, dentre os quais garantia do acesso e permanência de todos na escola. O problema de pesquisa que norteou este trabalho foi o de verificar a relação entre as políticas públicas educacionais para a educação especial traçadas pelo governo federal e a implementação das mesmas pela Secretaria Municipal de Educação de Marabá-Pará para a inclusão escolar dos alunos com necessidades educacionais especiais. A pesquisa se desenvolveu metodologicamente por meio de um estudo de caso de uma escola da rede municipal de educação de Marabá. Utilizamos como instrumentos de coleta de dados a entrevista semÍ-estruturada e a análise interpretativa das entrevistas e dos documentos cujos dados foram submetidos à análise de conteúdo. A partir de nossas análises e reflexões constatamos que a implantação da Educação Especial em âmbito municipal deu-se por força do processo de municipalização ocorrido em Marabá no de 2000. Ressaltamos que a implementação da mesma na prática, vem sofrendo muitos complicadores, dentre eles a falta de conhecimento de grande parte dos professores da rede em relação às questões mais específicas apresentadas pela maioria das deficiências de seus alunos, além da falta de infra-estrutura adequada das escolas para atender os alunos com necessidades educacionais especiais matriculados nas classes comuns.

Strengthening Families: Parents' Voices on Discipline and Child Rearing

This qualitative study conducted semi-structured, multi-session focus groups and interviews with twenty-seven participants to explore in-depth, participant constructs of child discipline and punishment methods and reasons for the continuing support for corporal punishment of U.S. children. The research assumed that parents want to parent well and utilized the strengths perspective as the instrument to listen to participants' voices. Narratives revealed that participants were thoughtful about discipline and parenting strategies and viewed their parent role as a serious commitment. Non-violent discipline strategies, particularly communication, were often used. However, parents generally framed use of physical punishment as “when children need spanking” versus articulating the view that corporal punishment is a choice. Parents were unfamiliar with risks associated with physical punishment and only three parents, as a result of their foster parent training, had ever heard, “Do not spank.” Participants enumerated services and recommendations that would support and inform their own parenting, as well as, benefit children and the eighty percent of women and men in the United States who become mothers and fathers. Recommendations included: creation of a national campaign to build on parent strengths and the intentionality of effective parenting; child development education and increased public awareness of positive discipline methods; parenting supports, including respite and venues for dialogue and discourse about parenting. Recommendations are intended to inform child welfare practice and policy, particularly child abuse prevention. Creating, funding, and implementing a national campaign as described would challenge the dominant child welfare paradigm from one currently perceived as punitive and focused on parents' deficits to a strengths-based paradigm that provides supports and assistance to parents and children.

Prediction of sepsis in the burn intensive care unit patient

Sepsis is a significant cause for multiple organ failure and death in the burn patient, yet identification in this population is confounded by chronic hypermetabolism and impaired immune function. The purpose of this study was twofold: 1) determine the ability of the systemic inflammatory response syndrome (SIRS) and American Burn Association (ABA) criteria to predict sepsis in the burn patient; and 2) develop a model representing the best combination of clinical predictors associated with sepsis in the same population. A retrospective, case-controlled, within-patient comparison of burn patients admitted to a single intensive care unit (ICU) was conducted for the period January 2005 to September 2010. Blood culture results were paired with clinical condition: "positive-sick"; "negative-sick", and "screening-not sick". Data were collected for the 72 hours prior to each blood culture. The most significant predictors were evaluated using logistic regression, Generalized Estimating Equations (GEE) and ROC area under the curve (AUC) analyses to assess model predictive ability. Bootstrapping methods were employed to evaluate potential model over-fitting. Fifty-nine subjects were included, representing 177 culture periods. SIRS criteria were not found to be associated with culture type, with an average of 98% of subjects meeting criteria in the 3 days prior. ABA sepsis criteria were significantly different among culture type only on the day prior (p = 0.004). The variables identified for the model included: heart rate>130 beats/min, mean blood pressure<60 mmHg, base deficit<-6 mEq/L, temperature>36°C, use of vasoactive medications, and glucose>150 mg/d1. The model was significant in predicting "positive culture-sick" and sepsis state, with AUC of 0.775 (p < 0.001) and 0.714 (p < .001), respectively; comparatively, the ABA criteria AUC was 0.619 (p = 0.028) and 0.597 (p = .035), respectively. SIRS criteria are not appropriate for identifying sepsis in the burn population. The ABA criteria perform better, but only for the day prior to positive blood culture results. The time period useful to diagnose sepsis using clinical criteria may be limited to 24 hours. A combination of predictors is superior to individual variable trends, yet algorithms or computer support will be necessary for the clinician to find such models useful. ^

(Table 1) Comparison of localities where Switzianthus (Anderson & Anderson) has been found with Dejerseya and Heidiphyllum occurrence

Fossil leaves of the Voltziales, an ancestral group of conifers, rank among the most common plant fossils in the Triassic of Gondwana. Even though the foliage taxon Heidiphyllum has been known for more than 150 years, our knowledge of the reproductive organs of these conifers still remains very incomplete. Seed cones assigned to Telemachus have become increasingly well understood in recent decades, but the pollen cones belonging to these Mesozoic conifers are rare. In this contribution we describe the first compression material of a voltzialean pollen cone from Upper Triassic strata of the Transantarctic Mountains. The cone can be assigned to Switzianthus Anderson & Anderson, a genus that was previously assumed to belong to an enigmatic group of pteridosperms from the Triassic Molteno Formation of South Africa. The similarities of cuticle and pollen morphology, together with co-occurrence at all known localities, indicate that Switzianthus most probably represents the pollen organ of the ubiquitous Heidiphyllum/Telemachus plant.

Brachiopod genera of the suborders Orthoidea and Pentameroidea, by Charles Schuchert and G. Arthur Cooper.

4. pt.1

Etching and other graphic arts;

Includes index.

The Rhine and its legends. A souvenir of the days of the American army of occupation in Germany,

On cover: Compliments of the American Y.M.C.A.

MicroRNA-155 promotes resolution of hypoxia-inducible factor 1α activity during prolonged hypoxia

The hypoxia-inducible factor (HIF) is a key regulator of the transcriptional response to hypoxia. While the mechanism underpinning HIF activation is well understood, little is known about its resolution. Both the protein and the mRNA levels of HIF-1a (but not HIF-2a) were decreased in intestinal epithelial cells exposed to prolonged hypoxia. Coincident with this, microRNA (miRNA) array analysis revealed multiple hypoxiainducible miRNAs. Among these was miRNA-155 (miR-155), which is predicted to target HIF-1a mRNA. We confirmed the hypoxic upregulation of miR-155 in cultured cells and intestinal tissue from mice exposed to hypoxia. Furthermore, a role for HIF-1a in the induction of miR-155 in hypoxia was suggested by the identification of hypoxia response elements in the miR-155 promoter and confirmed experimentally. Application of miR-155 decreased the HIF-1a mRNA, protein, and transcriptional activity in hypoxia, and neutralization of endogenous miR-155 reversed the resolution of HIF-1a stabilization and activity. Based on these data and a mathematical model of HIF-1a suppression by miR-155, we propose that miR-155 induction contributes to an isoform-specific negative-feedback loop for the resolution of HIF-1a activity in cells exposed to prolonged hypoxia, leading to oscillatory behavior of HIF-1a-dependent transcription. © 2011, American Society for Microbiology.

Small Ubiquitin-related Modifier (SUMO)-1 promotes glycolysis in hypoxia

Under conditions of hypoxia, most eukaryotic cells undergo a shift in metabolic strategy, which involves increased flux through the glycolytic pathway. Although this is critical for bioenergetic homeostasis, the underlying mechanisms have remained incompletely understood. Here, we report that the induction of hypoxia-induced glycolysis is retained in cells when gene transcription or protein synthesis are inhibited suggesting the involvement of additional post-translational mechanisms. Post-translational protein modification by the small ubiquitin related modifier-1 (SUMO-1) is induced in hypoxia and mass spectrometric analysis using yeast cells expressing tap-tagged Smt3 (the yeast homolog of mammalian SUMO) revealed hypoxia-dependent modification of a number of key glycolytic enzymes. Overexpression of SUMO-1 in mammalian cancer cells resulted in increased hypoxia-induced glycolysis and resistance to hypoxia-dependent ATP depletion. Supporting this, non-transformed cells also demonstrated increased glucose uptake upon SUMO-1 overexpression. Conversely, cells overexpressing the de-SUMOylating enzyme SENP-2 failed to demonstrate hypoxia-induced glycolysis. SUMO-1 overexpressing cells demonstrated focal clustering of glycolytic enzymes in response to hypoxia leading us to hypothesize a role for SUMOylation in promoting spatial re-organization of the glycolytic pathway. In summary, we hypothesize that SUMO modification of key metabolic enzymes plays an important role in shifting cellular metabolic strategies toward increased flux through the glycolytic pathway during periods of hypoxic stress. © 2011 by The American Society for Biochemistry and Molecular Biology, Inc.

Recreaciones contemporáneas de Pride and Prejudice de Jane Austen

No cabe duda de que Jane Austen (1775-1817) es a día de hoy no sólo una figura preeminente dentro de la literatura en lengua inglesa sino que de igual modo es una de las novelistas más conocidas por el gran público, un estatus que ha alcanzado sobre todo en los últimos veinte años. Gran parte de la popularidad de la que goza esta autora en el siglo veintiuno ha sido generada por las adaptaciones que se han realizado de sus novelas. A partir de mediados de los años noventa se desarrolla intensamente la tendencia de trasladar el argumento de sus obras, particularmente Pride and Prejudice, a un contexto contemporáneo. La presente tesis analiza este fenómeno y lleva a cabo un estudio exhaustivo de las recreaciones contemporáneas de Pride and Prejudice tanto en el medio literario como fílmico. Descartando el concepto de “fidelidad” como principio taxonómico, este trabajo presta atención a otras afinidades estructurales y temáticas para organizar el amplio corpus de reescrituras ya que éste es extraordinariamente amplio y dinámico. De esta forma tras un estudio del contexto histórico de Pride and Prejudice se presenta un análisis de las distintas tendencias que se pueden encontrar en las recreaciones contemporáneas de esta novela de Jane Austen. Así, se estudian en profundidad algunas de las novelas que abrieron el camino para la recontextualización de Pride and Prejudice en la actualidad como es el caso de Bridget Jones’s Diary (Helen Fielding, 1996). Igualmente también se consideran aquellas obras que trasladan el argumento de la novela de Jane Austen a otras culturas y sociedades como ocurre en Jane Austen in Boca (Paula Marantz-Cohen, 2002) y aquéllas que presentan a unas Janeites que escapan de la realidad de sus vidas a través de la literatura austeniana como sucede en Me & Mr Darcy (Alexandra Potter, 2007). Otra interesante tendencia que se analiza es la de las novelas que reescriben Pride and Prejudice para un lector eminentemente juvenil como sería el caso de Prom & Prejudice (Elizabeth Eulberg, 2011) así como las que realizan una clara labor de apropiación del legado de la autora británica como ocurre, por ejemplo, en Fitzwilliam Darcy Rock Star (Heather Lynn Rigaud, 2011). Estas mismas tendencias se pueden encontrar en las recreaciones audiovisuales de Pride and Prejudice y en el capítulo dedicado a ellas se procede al análisis de todas las reescrituras que se han realizado en este medio hasta el momento como, por ejemplo, Bride & Prejudice (Gurminder Chadha, 2004), Lost in Austen (Dan Zeff, 2008) o The Lizzie Bennet Diaries (Hank Green y Bernie Su 2012-13) entre otras. Todas ellas, sin duda, reflejan la vigencia y riqueza de un texto capaz de adaptarse a los más variados contextos que, a pesar de haber sido creado hace más de doscientos años, sigue siendo fuente de inspiración para multitud de creadores. Para finalizar se incluye un capítulo dedicado a la presencia de Jane Austen y de Pride and Prejudice en diversos ámbitos de la cultura popular, lo que pone de manifiesto el alcance de la autora y su obra en la sociedad actual.