Louis Lipsky papers 1898-1976

Consists of speeches, memoranda, correspondence, publications, clippings and ephemera pertaining to the life and career of a prominent American Zionist.

Harold Debrest papers 1905-1976

The collection contains miscellaneous writings, personal correspondence and memorabilia of Debrest, an American Jewish journalist, poet and business executive. Among the correspondents are Felix Adler and Harry Truman.

Performance of RT-PCR-ELISA for the detection of peste des petits ruminants virus

A highly sensitive and specific reverse transcription polymerase chain reaction enzyme linked immunosorbent assay (RT-PCR-ELISA) was developed for the objective detection of nucleoprotein (N) gene of peste des petits ruminants (PPR) virus from field outbreaks or experimentally infected sheep. Two primers (IndF and Np4) and one probe (Sp3) available or designed for the amplification/probing of the 'N' gene of PPR virus, were chosen for labeling and use in RT-PCR-ELISA based on highest analytical sensitivity of detection of infective virus or N-gene containing recombinant plasmid, higher nucleotide homology at the primer binding sites of the 'N' gene sequences available and the ability to amplify PPR viral genome from different sources of samples. RT-PCR was performed with unlabeled IndF and Np4 digoxigenin labeled primers followed by a microplate hybridization probe reaction with biotin labeled Sp3 probe. RT-PCR-ELISA was found to be 10-fold more sensitive than the conventional RT-PCR followed by agarose gel based detection of PCR product. Based on the Mean (mean +/- 3S.D.) optical density (OD) values of 47 RT-PCR negative samples, OD values above 0.306 were considered positive in RT-PCR-ELISA. A total of 82 oculo-nasal swabs and tissue samples from suspected PPR cases were analyzed by RT-PCR and RT-PCR-ELISA, which revealed 54.87 and 58.54% positivity, respectively. From an experimentally infected sheep, both RT-PCR and RT-PCR-ELISA could detect the virus from 6 days post-infection up to 9 days in oculo-nasal swabs. On post-mortem, PPR viral genome was detected in spleen, lymph node, lung, heart and liver. The correlation co-efficient between RT-PCR-ELISA OD values and either TCID50 of virus or molecules of DNA was 0.622 and 0.657, respectively. The advantages of RT-PCR-ELISA over the conventional agarose gel based detection of RT-PCR products are discussed. (c) 2006 Elsevier B.V. All rights reserved.

A new ELISA plate based microtiter well assay for mycobacterial topoisomerase I for the direct screening of enzyme inhibitory monoclonal antibody supernatants

Antigen specific monoclonal antibodies present in crude hybridoma supernatants are normally screened by ELISA on plates coated with the relevant antigen. Screening for inhibitory monoclonals to enzymes would require the evaluation of purified antibodies or antibody containing supernatants for their inhibition of enzyme activity in a separate assay. However, screening for inhibitory antibodies against DNA transacting enzymes such as topoisomerase I (topo I) cannot be done using hybridoma supernatants due to the presence of nucleases in tissue culture media containing foetal calf serum which degrade the DNA substrates upon addition. We have developed a simple and rapid screening procedure for the identification of clones that secrete inhibitory antibodies against mycobacterial topo I using 96 well ELISA microtiter plates. The principle of the method is the selective capture of monoclonal antibodies from crude hybridoma supernatants by topo I that is tethered to the plate through the use of plate-bound polyclonal anti-topo I antibodies. This step allows the nucleases present in the medium to be washed off leaving the inhibitor bound to the tethered enzyme. The inhibitory activity of the captured antibody is assessed by performing an in situ DNA relaxation assay by the addition of supercoiled DNA substrate directly to the microtiter well followed by the analysis of the reaction products by agarose gel electrophoresis. The validity of this method was confirmed by purification of the identified inhibitory antibody and its evaluation in a DNA relaxation assay. Elimination of all enzyme-inhibitory constituents of the culture medium from the well in which the inhibitory antibody is bound to the tethered enzyme may make this method broadly applicable to enzymes such as DNA gyrases, restriction enzymes and other DNA transaction enzymes. Further, the method is simple and avoids the need of prior antibody purification for testing its inhibitory activity. (C) 2010 Elsevier B.V. All rights reserved.

Quantitation of serological cross-reactivity between two geographical isolates of Oryctes baculovirus by a modified ELISA

An assay was developed for quantitation of the antigenic relationship between viruses, by modification of the indirect ELISA. The principle of this method is to estimate the epitopes not shared between the related viruses, after titration of the antibodies specific to the common epitopes as in a blocking ELISA. In practice, varying concentrations of purified virus are preincubated with a fixed dilution of heterologous or homologous antiserum and the unbound antibodies present in the mixture are back titrated with virus particles bound to microtitre plates. The antigenic relationship is described in terms of differentiation index (DI) and total antigenic reactivity (TAR). This method has been used to quantitate cross-reactivity between two geographically different isolates of Oryctes baculovirus.

Silva Fennica Vol. 10, 1976.

Julkaistu Silva Fennica Vol. 10(4) -numeron liitteenä.

Trade Blocs and the Cold War : The CMEA and the EC challenge, 1969-1976

The coherence of the Soviet bloc was seriously tested at the turn of the 1970s, as the Soviet Union and its allies engaged in intensive negotiations over their relations with the European Communities (EC). In an effort to secure their own national economic interests many East European countries began independent manoeuvres against the wishes of their bloc leader. However, much of the intra-bloc controversy was kept out of the public eye, as the battle largely took place behind the scenes, within the organisation for economic cooperation, the Council for Mutual Economic Assistance (CMEA). The CMEA policy-making process vis-à-vis the EC is described in this study with reference to primary archival materials. This study investigates the negotiating positions and powers of the CMEA member states in their efforts to deal with the economic challenge created by the progress of the EC, as it advanced towards the customs union. This entails an analysis of the functioning principles and performance of the CMEA machinery. The study traces the CMEA negotiations that began in 1970 over its policy toward the EC. The policy was finally adopted in 1974, and was followed by the first official meeting between the two organisations in early 1975. The story ends in 1976, when the CMEA s efforts to enter into working relations with the EC were seemingly frustrated by the latter. The first major finding of the study is that, contrary to much of the prior research, the Soviet Union was not in a hegemonic position vis-à-vis its allies. It had to use a lot of its resources to tame the independent manoeuvring of its smaller allies. Thus, the USSR was not the kind of bloc leader that the totalitarian literature has described. Because the Soviet Union had to spend so much attention on its own bloc-politics, it was not able to concentrate on formulating a policy vis-à-vis the EC. Thus, the Soviet leadership was dependent on its allies in those instances when the socialist countries needed to act as a bloc. This consequently opened up the possibility for the USSR s allies to manoeuvre. This study also argues that when the CMEA did manage to find a united position, it was a force that the EC had to reckon with in its policy-making. This was particularly the case in the implementation of the EC Common Commercial Policy. The other main finding of the study is that, although it has been largely neglected in the previous literature on the history of West European integration, the CMEA did in fact have an effect on EC decision-making. This study shows how for political and ideological reasons the CMEA members did not acknowledge the EC s supranational authority. Therefore the EC had no choice but to refrain from implementing its Common Commercial Policy in full.

Dissociation of monoclonal antibody-antigen complexes: Implications for ELISA procedures

A single step solid phase radioimmunoassay (SS-SPRIA) has been developed for human chorionic,gonadotropin (hCG) using monoclonal antibodies (MAb) from culture media adsorbed immunochemically on plastic tubes. The assays have been found to be very simple in terms of operation and do not demand purification of MAbs. Several MAbs which do not show any displacement in liquid phase RIA and ELISA provide a satisfactory SS-SPRIA. Our investigations revealed that the assumption regarding the stability of the primary Mab-Ag complex during incubation and washing steps in ELISAs is not strictly valid for dissociable MAbs. A comparison of different assay systems suggests that the single step SPRIA offers additional advantages over conventionally used multistep ELISA procedures and provides a quantitative probe for the analysis of epitope-paratope interactions.

Sapientia, 1976, Vol. XXXI, nº 122 (número completo)

Contenido: Verdad, historia y hermenéutica / Octavio N. Derisi – El lumen naturale en Santo Tomás y el essere ideale en Rosmini / William R. Darós – El constitutivo esencial de la persona / Octavio N. Derisi – Filosofía y desarrollo integral / Alberto Caturelli – La respuesta del hombre frente a lo existente : intuición y palabra / Osvaldo Francella – Notas y comentarios -- Bibliografía

Teoría y práctica de la poesía cinética en Composición de lugar (1976) de Amanda Berenguer

Resumen: Este trabajo propone un análisis de Composición de lugar (1976), el volumen que mejor representa la faceta experimental de la poesía de Amanda Berenguer (Montevideo, 1921-2010). Nos interesa examinar el texto a la luz de la noción de “poesía cinética” que desarrolló la autora y, a partir de esta coincidencia de teoría y praxis, rastrear sus vínculos con las búsquedas estéticas de otros autores y grupos de neovanguardia.

Teología, 1976, Tomo XIII nº 027-028 (número completo)

San Cipriano y el Donatismo, en la polémica antidonatista de san Agustín. Estudio histórico-patrístico / Rubén D. García -- La Iglesia y la existencia cristiana en la teología de San Buenaventura. II° parte / José Rovai -- Renovación de la Iglesia Ortodoxa Rusa a principios de este siglo / Domingo Krpan -- Pueblo, religión del pueblo e Iglesia / Lucio Gera -- El plan de la Epístola a los Gálatas / Luis H. Rivas -- Sagrada Congregación para la Educación Católica: La formación teológica de los futuros sacerdotes -- Notas bibliográficas

Mons. Enrique Angelelli, obispo de La Rioja (1968-1976) : recepción diocesana de su muerte: testimonios y valoraciones

Resumen: Este aporte intenta lograr una mirada sincrónica de la tragedia que se llevó de este mundo a Mons. Enrique Angelelli. La trama humana en su conjunto se desarrolla entre tristezas y alegrías y, también, lógicamente, la historia de la Iglesia posee los mismos condimentos. Pero, la clave está en la luz con la cual miramos estos acontecimientos que, en este caso, no sólo será científica o meramente sociológica sino, fundamentalmente, teológica. Así, al recoger los testimonios del pueblo de Dios, presentamos un hecho histórico reflejado a través del prisma de la fe.

[Discurso do Deputado Aldo Fagundes : Sessão da Câmara dos Deputados de 13 de setembro de 1976]

Dá conhecimento à Camara dos Deputados do inteiro teor do documento divulgado pelo Instituto dos Arquitetos do Brasil, onde se analisam as distorções sofridas pelo plano original de Brasília. Discorre sobre a importância da presença do homem no Planalto Central brasileiro, reverenciando a memória do ex-Presidente Juscelino Kubitschek na oportunidade em que evoca episódios ligados à fundação da cidade. Manifesta apreensão ante a ameaça de desumanização que paira sobre a comunidade brasiliense, conforme denuncia o referido documento. Enfatiza a necessidade da imediata institucionalização da ação comunitária em Brasília, que auxilie o Poder Público na solução dos problemas sociais que a cidade vem enfrentando. Traz à baila a questão da participação política do povo brasiliense, expondo as tendências que se vão esboçando, de um lado, pela criação de um Conselho Comunitário e, de outro, pela criação de um órgão de representação política propriamente dito, seja Câmara de Vereadores, seja Assembléia Legislativa. Reproduz em síntese as disposições gerais de projeto que apresentou propondo a criação do Conselho Comunitário de Brasília - COBRAS. Divulga o inteiro teor do parecer das Comissões Técnicas da Casa que já se manifestaram sobre o mencionado projeto. Anuncia a posição assumida pelo Presidente da Associação Comercial, Vicente de Paula Araújo, em favor da participação de Brasília nas decisões políticas e administrativas de interesse da cidade.

[Discurso do Deputado José Maurício : Sessão Conjunta do Congresso Nacional de 24 de março de 1976]

Congratula-se com o Diário de Brasília pela iniciativa de lançar ao debate a criação de uma representação política para o Distrito Federal.