Direct Observation of Millisecond to Second Motions in Proteins by Dipolar CODEX NMR Spectroscopy

We present a site-resolved study of stow (ms to s) motions in a protein in the solid (microcrystalline) state performed with the use of a modified version of the centerband-only detection of exchange (CODEX) NMR experiment. CODEX was originally based on measuring changes in molecular orientation by means of the chemical shift anisotropy (CSA) tensor, and in our modification, angular reorientations of internuclear vectors are observed. The experiment was applied to the study of stow (15)N-(1)H motions of the SH3 domain of chicken a-spectrin. The protein was perdeuterated with partial back-exchange of protons at labile sites. This allowed indirect (proton) detection of (15)N nuclei and thus a significant enhancement of sensitivity. The diluted proton system also made negligible proton-driven spin diffusion between (15)N nuclei, which interferes with the molecular exchange (motion) and hampers the acquisition of dynamic parameters. The experiment has shown that approximately half of the peaks in the 2D (15)N-(1)H correlation spectrum exhibit exchange in a different extent. The correlation time of the slow motion for most peaks is 1 to 3 s. This is the first NMR study of the internal dynamics of proteins in the solid state on the millisecond to second time scale with site-specific spectral resolution that provides both time-scale and geometry information about molecular motions.

CONTRA: Improving the performance of dynamic investigations in natural abundance organic solids by mirror-symmetric constant-time CODEX

We present a minor but essential modification to the CODEX 1D-MAS exchange experiment. The new CONTRA method, which requires minor changes of the original sequence only, has advantages over the previously introduced S-CODEX, since it is less sensitive to artefacts caused by finite pulse lengths. The performance of this variant, including the finite pulse effect, was confirmed by SIMPSON calculations and demonstrated on a number of dynamic systems. (C) 2007 Elsevier Inc. All rights reserved.

The codex Turnebi of Plautus,

Marginalia ascribed to Francois Duaren.

The miscellaneous dates of the Dresden Codex.

Mode of access: Internet.

The codex Turnebi of Plautus,

Mode of access: Internet.

The book of Isaiah according to the Septuagint (Codex Alexandrinus) /

"List of mss. containing Isaiah in Greek": v. 1, p. 56-58 ; v. 2, p. [xxxi]-xxxiii.

Bezae codex cantabrigiensis : being an exact copy, in ordinary type, of the celebrated Uncial Graeco-Latin manuscript of the four Gospels and Acts of the apostles /

Mode of access: Internet.

A system of English ecclesiastical law : extracted from the Codex juris ecclesiastici anglicani of the Right Reverend the lord bishop of London : for the use of young students in the universities, who are designed for holy orders /

Spine title: English ecclesiastical law.

Domesday studies: an analysis and digest of the Somerset survey (according to the Exon codex), and of the Somerset gheld inquest of A. D. 1084, as collated with, and illustrated by, Domesday.

v.1. Analysis and digest.--v.2. Tables.

Miscellanea Epigraphica Nubica IV: A New Edition of the Wizz Codex with an English Translation

The discovery of a complete codex in Coptic at the Nubian monastery of Qasr el Wizz has attracted the attention of several scholars. The edition of Péter Hubai has provided a base for all subsequent analyses of the text and already two related publications have been announced. The present contribution is the result of reading the Wizz Codex in a study group at the University of Bergen on Coptic texts, yielding an updated transcription and translation of the text.