Epstein-Barr virus (EBV) nuclear antigen (EBNA)-4 mutation in EBV-associated malignancies in three different populations

Different ethnic groups with a high human leukocyte antigen (HLA)-A11 prevalence have been shown to experience a high rate of Epstein-Barr virus (EBV) infection, EBV-associated malignancies, and Epstein-Barr nuclear antigen (EBNA)-4 mutations. The epitopes 393-408 and 416-424 of EBNA-4 are major antigenic epitopes that elicit an HLA-A11 cytotoxic T lymphocyte (CTL) response to EBV infection. Mutations selectively involving one or more nucleotide residues in these epitopes affect the antigenicity of EBNA-4, because the mutant EBV strains are not recognized by the HLA-A11-restricted CTLs. To investigate these mutations in common EBV-associated malignancies occurring in different populations, we studied the mutation rate of epitopes 393-408 and 416-424 of EBNA-4 in 25 cases of EBV-associated Hodgkin's disease (HD), nine cases of AIDS-related non-Hodgkin's lymphoma, and 37 cases of EBV-associated gastric carcinoma (GC) from the United States, Brazil, and Japan. We found one or more mutations in these two epitopes in 50% (6/12) of United States HD, 15% (2/13) of Brazilian HD, 50% (6/12) United States GC and 28% (7/25) Japanese GC, and 22% (2/9) of United States AIDS-lymphoma. Similar mutations were found in 30% (3/10) of United States reactive, 0% (0/6) of Brazilian reactive, and 25% (2/8) Japanese reactive tissues. The most frequent amino acid substitutions were virtually identical to those seen in previously reported isolates from EBV-associated nasopharyngeal carcinomas and Burkitt's lymphomas occurring in high prevalence HLA-A11 regions. However, only 2/28 (7%) mutations occurred in HLA-A11-positive patients. Our studies suggest that: 1) EBNA-4 mutations are a common phenomenon in EBV-associated HD, GC, and AIDS-lymphoma; 2) the mutation rate does not vary in these geographic areas and ethnic groups; 3) EBNA-4 mutations in EBV-associated United States and Brazilian HD, United States and Japanese GC, and United States AIDS lymphomas are not related to patients' HLA-A11 status.

Keystone Club Of Rock Hill Records - Accession 286 - M122 (155)

The Keystone Club of Rock Hill Records consist of constitutions, minutes, yearbooks and study program booklets from Winthrop’s Extension Dept. in the early 1920s. The Keystone Club of Rock Hill, SC was organized in 1914 as a women’s study group. The club was a charter member of Rock Hill City Federation of Women's Clubs and was a member of the State Federation of Women's Clubs and of the General Federation of Women's Clubs.

The Probe, Issue # 155 -- June 1995

Understanding the Eastern Coyote - Part II, by Thomas N. Tomsa, Jr., USDA-APHIS-ADC, Pennsylvania Book Review - "The Dirt Hole and Its Variations" All Texas Counties Quarantined for Rabies Ravenous Vultures Decimating Sheep & Calves on the East Coast Four-Year-Old Dies of Rabies The National Urban Wildlife Management Association (NUWMA) officially merged with NADCA, to create one larger, more effective organization to work for professional Animal Damage Control. State-Endangered Species: Meaningful Management or Preservationist Politics?, by Richard B. Chipman, Wildlife Biologist, USDA-APHIS-ADC, Vermont NADCA Membership Meeting Trapping Weasels
Jack H. Berryman 1995 Leopold Award Winner

Future prospects for SPECT imaging using the radiolanthanide terbium-155 — production and preclinical evaluation in tumor-bearing mice

We assessed the suitability of the radiolanthanide 155 Tb (t1/2 = 5.32 days, Eγ = 87 keV (32%), 105 keV (25%)) in combination with variable tumor targeted biomolecules using preclinical SPECT imaging. Methods 155Tb was produced at ISOLDE (CERN, Geneva, Switzerland) by high-energy (~ 1.4 GeV) proton irradiation of a tantalum target followed by ionization and on-line mass separation. 155 Tb was separated from isobar and pseudo-isobar impurities by cation exchange chromatography. Four tumor targeting molecules – a somatostatin analog (DOTATATE), a minigastrin analog (MD), a folate derivative (cm09) and an anti-L1-CAM antibody (chCE7) – were radiolabeled with 155 Tb. Imaging studies were performed in nude mice bearing AR42J, cholecystokinin-2 receptor expressing A431, KB, IGROV-1 and SKOV-3ip tumor xenografts using a dedicated small-animal SPECT/CT scanner. Results The total yield of the two-step separation process of 155 Tb was 86%. 155 Tb was obtained in a physiological l-lactate solution suitable for direct labeling processes. The 155 Tb-labeled tumor targeted biomolecules were obtained at a reasonable specific activity and high purity (> 95%). 155 Tb gave high quality, high resolution tomographic images. SPECT/CT experiments allowed excellent visualization of AR42J and CCK-2 receptor-expressing A431 tumors xenografts in mice after injection of 155 Tb-DOTATATE and 155 Tb-MD, respectively. The relatively long physical half-life of 155 Tb matched in particular the biological half-lives of 155 Tb-cm09 and 155 Tb-DTPA-chCE7 allowing SPECT imaging of KB tumors, IGROV-1 and SKOV-3ip tumors even several days after administration. Conclusions The radiolanthanide 155 Tb may be of particular interest for low-dose SPECT prior to therapy with a therapeutic match such as the β--emitting radiolanthanides 177Lu, 161 Tb, 166Ho, and the pseudo-radiolanthanide 90Y.

Ms. hebr. oct. 155 - [Sammelband in 2 Teilen: Handschriftenkonvolut]

Vorbesitzer: Gerschon ben Man Schaz;; Eljāqīm Carmoly; Abraham Merzbacher; Abraham Levi Segal

Bericht der Fach-Kommission für Justiz und Gesetzgebung über den Gesetz-Entwurf betreffend die Aufhebung der §§. 151. bis 155. Tit. 20. Thl. II. des allgemeinen Landrechts ...

Welsch (Projektarbeiter): Gesetzentwurf auf Aufhebung der Paragraphen 151 bis 155 des Preußischen Allgemeinen Landrechts ('Erweckung von Unzufriedenheit gegen die Regierung durch frechen Tadel oder Verspottung der Landesgesetze') und Vorschlag ihrer Neufassung in folgender Form: Wer durch Reden oder Schriften wissentlich Unwahrheiten verbreitet, welche geeignet sind, Haß oder Verachtung dem Staat gegenüber hervorzurufen, wird mit Freiheitsstrafe bis zu sechs Monaten bestraft

Haus Jacob Emdens [Illustration] : Altona, Breitestraße 155, mit dem Dachschutz nach biblischer Vorschrift

Scan von Monochrom-Mikroform

Ms. Barth. 155 - Ordo iudiciarius

Vorbesitzer: Bartholomaeusstift Frankfurt am Main

Na 50 Nachlass Arthur Schopenhauer - 'Schopenhauer-Archiv', 155 - Rechnung der Fürstlichen privaten Hofbuchhandlung Rudolstadt

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Na 50 Nachlass Arthur Schopenhauer - 'Schopenhauer-Archiv', 941 - Empfehlung von Münchner Rechtsanwälten sowie biographische Angaben zu Arthur Schopenhauer

u.a. Parerga und Paralipomena; Biographie; Nachlass; Idealismus; Romantik; Humanismus; Materialismus; Christentum; Rechtsphilosophie; Todesstrafe; Stadtgericht Frankfurt;