A two-step mechanism for epigenetic specification of centromere identity and function

The basic determinant of chromosome inheritance, the centromere, is specified in many eukaryotes by an epigenetic mark. Using gene targeting in human cells and fission yeast, chromatin containing the centromere-specific histone H3 variant CENP-A is demonstrated to be the epigenetic mark that acts through a two-step mechanism to identify, maintain and propagate centromere function indefinitely. Initially, centromere position is replicated and maintained by chromatin assembled with the centromere-targeting domain (CATD) of CENP-A substituted into H3. Subsequently, nucleation of kinetochore assembly onto CATD-containing chromatin is shown to require either the amino- or carboxy-terminal tail of CENP-A for recruitment of inner kinetochore proteins, including stabilizing CENP-B binding to human centromeres or direct recruitment of CENP-C, respectively.

Poetry" sculpture on base of front facade flanking step

Albert Kahn, architect. Building completed 1924. Named James Burrill Angell Hall. Sometimes called Literary College. Interior ceiling decorations: Di Lorenzo Studios, N.Y. On verso: University of Michigan News Service

History" sculpture on base of front facade flanking step

Albert Kahn, architect. Building completed 1924. Named James Burrill Angell Hall. Sometimes called Literary College. Interior ceiling decorations: Di Lorenzo Studios, N.Y. On verso: University of Michigan News Service

Arts" sculpture on base of front facade flanking step

Albert Kahn, architect. Building completed 1924. Named James Burrill Angell Hall. Sometimes called Literary College. Interior ceiling decorations: Di Lorenzo Studios, N.Y. On verso: University of Michigan News Service

Stability and convergence of general multistep and multivalue methods with variable step size.

Thesis--University of Illinois.

An attempt to find an a priori measure of step size /

Project No. 711151.01.

Investigation of one-step methods for integro-differential equations /

Includes bibliographies (p. 11).

Stability of variable-step methods for ordinary differential equations /

Bibliography: leaf 11.

Test patterns NBS-28 and NBS-28A : random fault interconnect step coverage and other structures /

S/N 003-003-02297-7.

The step-mother, a tragedy.

Mode of access: Internet.

Philosophy" sculpture on base of front facade flanking step

Albert Kahn, architect. Building completed 1924. Named James Burrill Angell Hall. Sometimes called Literary College. Interior ceiling decorations: Di Lorenzo Studios, N.Y.

Variable Step Maximum Power Point Tracking for Photovoltaic Modules with Embedded Temperature Sensing

Thesis (Master's)--University of Washington, 2016-06