Efeito da concentra����o citost��tica de 5-fluorouracil sobre a imunogenicidade de c��lulas de c��ncer colorretal murino MC-38

A quimioterapia �� o tratamento padr��o para pacientes com os mais variados tipos de c��ncer, (sendo o 5 Fluorouracil -5Fu- a droga de escolha no caso do c��ncer colorretal) mas ela est�� associada a efeitos colaterais que podem ser muito severos. A exposi����o de c��lulas tumorais a agentes antineopl��sicos, em dose baixa e n��o citot��xica, pode torn��-las mais imunog��nicas, enquanto que a exposi����o de c��lulas dendr��ticas (DC) a esses agentes antineopl��sicos pode aumentar sua capacidade de induzir resposta antitumoral in vitro. O objetivo deste estudo �� verificar se o tratamento in vitro de c��lulas tumorais MC38 com 5-Fu (dose n��o citot��xica) pode induzir a express��o de mol��culas que aumentem suas caracter��sticas imunog��nicas, fazendo com que elas sejam mais facilmente identificadas pelo sistema imune. Para isso, camundongos da linhagem C57/Bl-6 foram inoculados subcutaneamente com c��lulas MC38 e sete dias depois foram vacinados com DC sensibilizadas com ant��genos tumorais obtidos a partir da lise de c��lulas MC38 mantidas em cultura e previamente tratadas com 5-Fu, em dose n��o citot��xica (DC-5Fu), para que a performance das DC sensibilizadas pudesse ser comparada �� performance das DC selvagens

Pol��ticas p��blicas, protagonismo social e comunica����o na expans��o da cultura no Brasil

Este trabalho analisa aspectos do contexto das pol��ticas p��blicas culturais contempor��-neas relacionadas com as a����es de mobiliza����o que envolvem a sociedade civil. A an��-lise engloba conhecimento sobre o papel do profissional de Rela����es P��blicas na con-tribui����o da execu����o de pol��ticas culturais que incentivem o protagonismo social atra-v��s do Programa Cultura Viva, desenvolvido pelo Minist��rio da Cultura, com a imple-menta����o de Pontos e Pont��es de Cultura, e no ��mbito de movimentos sociais indepen-dentes. Os resultados apontam a import��ncia estrat��gica do Rela����es P��blicas na expan-s��o das pol��ticas p��blicas culturais

Avalia����o do gerenciamento e disposi����o final dos res��duos qu��micos de laborat��rios do IGCE, UNESP - Rio Claro/SP

Laboratory of Chemical Waste Management programs are being implemented in various universities of the country and the world, in recognition of the urgent need to change the reality of disregard for the environment, together with objective responsibility of the generator, and especially the awareness of sustainability. Analysis and research laboratories involve a range of waste in developing their studies with intrinsic characteristics related to their form of generation. The amount of waste generated in this segment is negligible compared to industrial activities, but the environmental issue is that these residues do not have a standard technique for treatment due to the potential variation of its composition. This research project, aiming their suitability and continuous improvement, aims to diagnose and analyze the current situation of the management and disposal of chemical waste generated by the laboratories IGCE UNESP, Campus Rio Claro / SP. Universities, through their research, teaching and extension, end up generating potentially hazardous chemicals that may contaminate the environment when they are disposed of improperly, with no concern for the environment and / or public health. Aiming to assist in improvements with respect to this issue, the purpose of this study is to understand the management and disposal of chemical waste from the IGCE, laboratories UNESP - Rio Claro / SP, in order to discuss the situation of such waste, and present proposals to reduce generation of the same and minimizing the environmental impact, thereby increasing the local hygiene through proper disposal. The research instrument used interviews, questionnaires, review of recent literature and observations were made in order to develop proposals for the management and disposal of waste. These proposals based on prevention and corrective control, where the preventive approach aims at lifting techniques and actions to reduce the generating sources and...

Caracteriza����o de Bacteri��fagos Infectando Isolados Cl��nicos de Pseudomonas aeruginosa Armazenados em uma Cole����o de Cultura

To isolate, to concentrate and to purify bacteriophages from isolates of P. aeruginosa; To observe the capacity of bacteriophages to infect isolates of P. aeruginosa susceptible and multiresitant to antimicrobial; To caractherize bacteriphages by electronic microscopy techniques. 10 isolates of Pseudomonas aeruginosa from LEMC culture collection were submitted to the experiments of ideal temperature for the lyse region appearance in the MaConkey culture plate and 2 extraction methods for the concentration of the phages, clorophorm (Silankorva) and filtration plus centrifugation (Bergan). Three infected clinical isolates of multiresistant P. aeruginosa an one susceptible isolate ( PA01) were evaluated by 3 transmission electron microscopy techniques to caractherize phages morphologically (���on grid���, ���on drop��� and direct extraction from the lyse region of the culture plate). The ideal temperature to obtain lyses region was 37��C. The stock solutions, obtained through the methodologies of Sillankorva and Bergan, had satisfactory results in infecting the multiresistant isolate and the negative control. Among the 3 techniques of electronic microscopy tested the direct from the lyse plate was the best to obtain the micrography of the phages

Avalia����o da qualidade de ��leos de fritura utilizados em restaurante universit��rio

Frying is a widely used method of preparing food, because it is a very fast and convenient process, and because it provides specific characteristics of color, flavor, odor, and texture, besides having great consumer acceptance. Therefore, the interest in the physiological effects that oils heated at high temperatures can cause to the human body arises. The aim of this work was to analyze the levels of frying used oil alteration in different kinds of foods served at the university restaurant at ���Instituto de Bioci��ncias, Letras e Ci��ncias Exatas��� (IBILCE), in S��o Jos�� do Rio Preto-SP. In addition, it aims to make the restaurant aware of the good ways to fry and the commitment of providing good quality food to students and professionals of the Institute. With this purpose, determination of total polar compounds, conjugated dienoic acids, peroxide value, and fatty acid profile were analyzed. Two rapid tests were also used: 3M Fat Monitor and Oil Test kit. The results were compared with limits recommended for the disposal of oils and fats used in frying by the Brazilian Health Surveillance Agency (ANVISA), by other countries, and by other researches. Thus, it was concluded that the university restaurant provides the consumers with fried food of good quality, since none of the analyses showed results above recommended, although there is a need of improvement in the quality control of the oil used, in order to avoid unnecessary costs to the university restaurant.

An��lise comparativa das altera����es radiogr��ficas observadas no osteossarcoma e na osteomielite em c��es

O exame radiogr��fico �� um procedimento simples e relativamente barato que pode ser utilizado para observa����o de estruturas ��sseas e tecidos moles, incluindo suas altera����es, sendo muito empregado em casos de suspeita de aumento de volume ��sseo devido a tumores e inflama����es. Osteossarcoma e osteomielite, al��m de causarem a altera����o radiogr��fica acima citada, possuem imagem radiogr��fica muito semelhante na maioria dos casos, como prolifera����o e/ou lise ��ssea, presen��a do tri��ngulo de Codman e perda do padr��o trabecular ��sseo, al��m de sintomas cl��nicos similares como claudica����o e poss��vel presen��a de fraturas patol��gicas. A necessidade de se realizar outros m��todos de exame complementar paralelos �� radiografia �� muito importante na determina����o de um diagn��stico seguro, o que implica em um tratamento precoce da doen��a evitando-se assim grande piora no estado geral do paciente. Esta revis��o sistem��tica de literatura visa a compara����o radiogr��fica entre osteomielite e osteossarcoma em c��es, para auxilio do m��dico veterin��rio na diferencia����o de seu diagn��stico.

O pagamento por desempenho no contexto das reformas educacionais paulistas

This article presents the results of a research that has as its analysis focus the payment by performance, represented in the public educational system of the S��o Paulo State by the bonus-merit. The bonus, such as other measures implemented in the last decade, is part of neoliberal reforms that were predominant in the public educational system of the State. The article intends to debate the implications of this mechanism for the teaching from a bibliographic review about the topic as well as from the interpretation of data originated from a qualitative empirical investigation based on semi-structured interviews with second cycle teachers of high and middle schools of three regional departments of education. The results revealed an influence of performative and managerial elements in the organizational work of schools and teachers. Moreover, they showed that the payment by performance has not contributed for the improvement of the work conditions and for the career of the teachers, has a very limited impact to change the quality of education and thus represent a teacher accountability mechanism.

Surface Characteristics of Reciprocating Instruments Before and After Use - A SEM Analysis

Conselho Nacional de Desenvolvimento Cient��fico e Tecnol��gico (CNPq)

Merleau-Ponty e a condi����o do natural em n��s

In this paper, the aim is to highlight and analyze certain moments in the work of Merleau-Ponty when discussions took place about the condition of the natural in us. We mainly focus on the observed opposition between the evolutionist viewpoint adopted in The structure of Behavior and the perspective in the Phenomenology of Perception, based on the body as expression. We also look at studies on infantile prematuration and mention the characterization of the human body in the philosopher���s courses on the notion of nature. We underline the expressive dimension of the natural in us, which emerges from Merleau-Ponty���s formulations. In addition, we evidence the importance of psychoanalysis in the Merleau-Ponty���s treatment of the corporeity problem.

Vari��veis de impacto na queda da mortalidade infantil no Estado de S��o Paulo, Brasil, no per��odo de 1998 a 2008

This is an ecological, analytical and retrospective study comprising the 645 municipalities in the State of Sao Paulo, the scope of which was to determine the relationship between socioeconomic, demographic variables and the model of care in relation to infant mortality rates in the period from 1998 to 2008. The ratio of average annual change for each indicator per stratum coverage was calculated. Infant mortality was analyzed according to the model for repeated measures over time, adjusted for the following correction variables: the city's population, proportion of Family Health Programs (PSFs) deployed, proportion of Growth Acceleration Programs (PACs) deployed, per capita GDP and SPSRI (Sao Paulo social responsibility index). The analysis was performed by generalized linear models, considering the gamma distribution. Multiple comparisons were performed with the likelihood ratio with chi-square approximate distribution, considering a significance level of 5%. There was a decrease in infant mortality over the years (p < 0.05), with no significant difference from 2004 to 2008 (p > 0.05). The proportion of PSFs deployed (p < 0.0001) and per capita GDP (p < 0.0001) were significant in the model. The decline of infant mortality in this period was influenced by the growth of per capita GDP and PSFs.

Adesivo tecidual de fibrina e sua aplica����o na implantodontia

The purpose of this literature review was to evaluate the use of fibrin tissue adhesive in implant dentistry. Materials and Methods: A literature search of Medline-PubMed for articles published, describing the use of fibrin tissue adhesive in implant dentistry was performed and articles were critically reviewed. Results: The literature review reveals clinical trials and experimental studies with regard to the use of fibrin tissue adhesive in implant dentistry. This material consists of 2 components: highly purified, freeze-dried human fibrinogen with factor XIII and a starter solution containing human thrombin. Clotting factor XIII is admitted for reinforcement of the fibrin network. The components are reconstituted before use and when mixed form a clot by mimicking the terminal phase of the physiological clotting cascade. Several studies showed that fibrin tissue adhesive is fully absorbed by macrophages within 2 weeks of application. Adhesive fibrin tissue may be used for to prevent bone loss, to create contour in the periimplant soft tissue and osseous tissue, to sculpt emergence profile for prosthetic components and to mimic tissue architecture. In the last years fibrin tissue adhesive also find use as material for the controlled delivery of drugs and biologics. Conclusions: The fibrin tissue adhesive presents good properties such as biocompatibility, hemostatic properties and ability to break down like the physiologic clot. This material, alone or associated with other materials, can be used with the implants immediately after extraction. In this condition it brings the necessary anchoring and efficient maintenance of osseous/mucosal contour, which it is important for the clinical success.

The influence of the alveolar ridge shape on the stress distribution in a free-end saddle removable partial denture supported by implant

The alveolar ridge shape plays an important role in predicting the demand on the support tooth and alveolar bone in the removable partial denture (RPD) treatment. However, these data are unclear when the RPD is associated with implants. This study evaluated the influence of the alveolar ridge shape on the stress distribution of a free-end saddle RPD partially supported by implant using 2-dimensioanl finite element analysis (FEA). Four mathematical models (M) of a mandibular hemiarch simulating various alveolar ridge shapes (1-distal desceding, 2- concave, 3-horizontal and 4-distal ascending) were built. Tooth 33 was placed as the abutment. Two RPDs, one supported by tooth and fibromucosa (MB) and other one supported by tooth and implant (MC) were simulated. MA was the control (no RPD). The load (50N) were applied simultaneously on each cusp. Appropriate boundary conditions were assigned on the border of alveolar bone. Ansys 10.0 software was used to calculate the stress fields and the von Mises equivalent stress criteria (��vM) was applied to analyze the results. The distal ascending shape showed the highest ��vM for cortical and medullar bone. The alveolar ridge shape had little effect on changing the ��vM based on the same prosthesis, mainly around the abutment tooth.

Avalia����o das altera����es hemorr��gicas e tromboemb��licas em c��es com doen��a renal cr��nica

Funda����o de Amparo �� Pesquisa do Estado de S��o Paulo (FAPESP)

Avalia����o funcional pr�� e p��s programa de exerc��cio f��sico de pacientes em hemodialise

Model Study: An experimental study Introduction: Chronic Kidney Disease (CKD) refers to a syndromic diagnosis which leads to a progressive and irreversible loss of renal function. A hemodialysis patient may have limitations in functional capacity, pulmonary function and respiratory musclular strength impacting in quality of life. Objective: To evaluate the effects of an exercise program on pulmonary function, functional capacity, quality of life and pain in patients undergoing hemodialysis. Methodology: The study included 28 patients of both genders, women and men aged between 40 and 60 years undergoing dialysis at the Kidney Institute, Santa Casa de Misericordia in Presidente Prudente-SP. Primary outcomes included respiratory muscular strength measurements assessed by manovacuometry. The functional capacity was evaluated by a six minute walking test. A life quality questionnaire was applied to evaluate quality of life (SF36-KDQOL). Lung function was evaluated by spirometry. Pain was assessed by a visual analogue scale. The exercise program consisted of training 3 times a week for 40 minutes on hemodialysis during eight weeks. At the end of the program all patients were reassessed. Results: There was no significant difference in the values of FVC and FEV1 before and after the exercise program as well as the index Tiffenau. The value of post MIP was significantly higher than the value obtained in the pre program. For variable MEP no significant difference was found. Functional capacity evaluations showed that there were no significant differences (p> 0.05). The evaluation of quality of life, about the domains of specific areas of CKD showed statistical significance when comparing the list of symptoms and problems with overloading of renal disease and professional role. Indicators related to pain were significantly reduced after the program (P <0.05). Discussion: A chronic kidney patient faces complex situations of physical, social and financial aspects. Although no statistically significant results were found in all variables, the study corroborates to others found in the literature, which suggests that an exercise program can be positive for this population. Conclusion: Although lung capacity and functional capacity did not submit changes to the end of the study, reduced levels of pain, fatigue and dyspnea suggest improvement in functional performance after exercise programs.

Televis��o digital terrestre na agenda do MERCOSUL

El escenario de la digitalizaci��n de los medios de comunicaci��n, especialmente la televisi��n terrestre, y una pol��tica para el desarrollo de contenidos audiovisuales entraron en la agenda del MERCOSUR. Cambiar de el est��ndar de TV fue solamente el argumento embrionario para un conjunto de acciones destinadas a dar densidad a las iniciativas conjuntas de Argentina, Brasil, Paraguay y Uruguay en la j��ven integraci��n pol��- tica y econ��mica del Cono Sur. As��, nos propone- mos la reflexi��n en torno a las pol��ticas p��blicas que provocan la implementaci��n de la televisi��n digital en los pa��ses miembros del MERCOSUR intentando delinear los intereses pol��ticos, la reestructuraci��n del mercado regional y el establecimiento de modelos, patrones y sistemas de cooperaci��n entre los pa��ses miembros. El an��lisis nos permite inferir que los gobiernos con matices de de centroizquierda de MERCOSUR optaron por la integraci��n de las pol��ticas p��bli- cas para fortalecer de la hegemon��a del bloque en la innovaci��n tecnol��gica, el desarrollo de contenido compartido y el fortalecimiento del capitalismo inmaterial.