Hauraita työmarkkina-asemia ja marginaalisia mahdollisuuksia

Kirjallisuusarvostelu

Parisuhteessa ratkotaan muistoja

Kirjallisuusarvostelu

Asiakaspalveluprosessin kuvaus ja laadun arviointi

Prosessien kuvaaminen yrityksissä on olennainen osa niiden kehittämisen kannalta. Prosessien kehittämistä varten niitä ohjaavien mittareiden valinta on keskeisessä roolissa. Tutkimuksen kohteena oleva kohde-yritys haluaa luoda teknisen asiakaspalvelun prosessin kuvauksen sekä valita mittarit ohjaamaan prosessia. Lisäksi palvelulle halutaan tehdä laadun arviointi. Diplomityön tavoitteena oli luoda kohdeyrityksen teknisestä asiakaspalvelusta prosessin kuvaus sekä, sitä ohjaavat, mittarit sairaalahygienian välinehuollon liiketoimintasektorille. Tavoitteena oli myös suorittaa palvelulle laadun arviointi hyödyntäen teoriasta löydettävän työkalun avulla. Työssä tutkittiin teorian osalta prosessien kehittämiseen ja kuvaamiseen liittävää tietoa, palvelun laatuun liittyviä seikkoja sekä menetelmää arvioida laatua SERVQUAL kyselyn avulla. Laatua arvioitaessa verrattiin asiakkaiden palvelu-kokemusten ja odotusten välistä eroa. Työ tuotti kohdeyrityksen tekniselle asiakaspalvelun sairaala puolelle prosessin kuvauksen sen palvelutoiminnalle. Kaksi mittaria, laatu ja tuottavuus, saatiin ohjaamaan palveluprosessia. SERVQUAL laatukyselyn avulla havaittiin, että asiakkaiden palvelukokemukset alittivat odotukset monella palvelun osa-alueella.

Uma casa e seus segredos: a formação de olhares sobre o Museu Mariano Procópio

Esse trabalho busca refletir as possibilidades e desafios da educação histórica em museus. Com este objetivo, selecionamos o Museu Mariano Procópio (Juiz de Fora-MG) como cenário para nossa proposta. O produto proposto é composto por três partes principais, a saber, o livreto sobre o MMP, as pranchas pedagógicas sobre seu acervo e, por fim, as fichas para adultos, com informações adicionais. Em um primeiro momento, analisamos o processo de constituição do Museu Mariano Procópio e seu acervo, a partir de uma perspectiva histórica. A consecução do produto foi realizada a partir de diálogos teóricos entre as pesquisas do ensino de História, da educação e da museologia. Com base na leitura de dissertações e teses sobre educação em museus, mapeamos brevemente as principais questões colocadas pelos pesquisadores a partir dos anos 80. Em seguida, apresentamos nossos pressupostos de educação histórica, aliado a uma discussão sobre a aprendizagem a partir de fontes primárias. A proposta de um produto pedagógico, destinado ao público infantil, nos levou à sondagem de outros materiais produzidos pelos museus. Por fim, apresentamos o processo de produção do material pedagógico.

Expression pattern of claudins 1 and 3-an auxiliary tool in predicting behavior of mucoepidermoid carcinoma of salivary gland origin

Claudins (CLDNs) are a family of membrane proteins important for permeability of tight junctions. They have also been implicated in carcinogenesis and tumor progression. We analyzed patterns of distribution and intensity of expression of CLDNs 1, 3, 4, and 7 in mucoepidermoid carcinoma (MEC) of salivary gland in 39 patients. Correlations between the expression of CLDNs, tumor grade, and survival were explored. In immunohistochemical analysis, high expression of CLDN 1 was seen in low-grade MEC, and it appeared to be a suitable auxiliary marker of good prognosis. It classified MEC similarly to histological grading in 89.7% of cases (p=0.001). High CLDN 3 expression was seen in intermediate-and high-grade MEC, while it was low in low-grade MEC. CLDN 3 intensity correctly categorized tumors into grades in 71.8% of cases (p=0.017). However, in multivariate analysis CLDN 1 and CLDN 3 did not achieve significance over tumor grade in predicting patient behavior. We conclude that analysis of staining intensities of CLDN 1 and 3 is useful as an auxiliary diagnostic and prognostic tool in patients with salivary gland MEC.

2002 Ice Hockey Team

Front Row (L-R): Associate Head Coach Mel Pearson, Josh Blackburn, Jay Vancik, Mike Cammalleri, Head Coach Red Berenson, John Shouneyia, Craig Murray, Kevin O'Malley, Assistant Coach Billy Powers.

Second Row (L-R): Justin Spurlock, Milan Gajic, Eric Werner, David Wyzgowski, Joe Kautz, Brad Fraser, Jed Ortmeyer, Andy Burnes, Mike Roemensky, Mark Mink, J.J. Swistak.

Third Row (L-R): Michael Woodford, Charlie Henderson, Dwight Helminen, Reilly Olson, Jason Ryznar, Brandon Rogers, Eric Nystrom, David Moss, Nick Martens, Mike Komisarek.

Fourth Row (L-R): Video Coordinator Ryan Rezmierski, Administrative Assistant Brian Wiseman, Athletic Trainer Rick Bancroft, Student Athletic Trainer Jesse Johnson, Equipment Manager Ian Hume, Assistant Equipment Manager David Brooks.

2004 Ice Hockey Team

Bottom row (I. to r.): Associate head coach Mel Pearson, Al Montoya, Joe Kautz, Brandon Rogers, Head coach Red Berenson, Andy Burnes, Eric Nystrom, David Wyzgowski, Noah Ruden, Assistant coach Billy Powers.

Second row: Mike Mayhew, Brandon Kaleniecki, Andrew Ebbett, Mike Brown, Jeff Tambellini, Milan Gajic, Charlie Henderson, Eric Werner, T.J. Hensick, Dwight Helminen.

Third row: Matt Hunwick, Michael Woodford Jr., David Rohlfs, Tim Cook, Jason Ryznar, David Moss, Nick Martens, Reilly Olson, Jason Dest.

Back row: Rick Bancroft, David Harlock, Tom Kahila, Josh Richelew.

Inhibition of transglutaminase 2 enzymatic activity ameliorates the anti-angiogenic effects of coeliac disease autoantibodies

Objective. Earlier work has demonstrated that serum autoantibodies from coeliac patients targeted against transglutaminase 2 (TG2) inhibit in vitro angiogenesis. The aim of this study was to establish whether coeliac patient-derived monoclonal TG2-targeted antibodies produced by recombination technology exert similar anti-angiogenic effects to serum-derived coeliac autoantibodies. In addition, we studied whether the monoclonal patient autoantibodies modulate endothelial cell TG2 activity and whether such modulation is related to the anti-angiogenic effects. Material and methods. The influence of coeliac patient-derived monoclonal TG2-targeted antibodies on endothelial cell tubule formation was studied using a three-dimensional angiogenic cell culture model. Endothelial cell TG2 enzymatic activity was determined by means of a live-cell enzyme-linked immunosorbent assay. Results. Coeliac patient-derived monoclonal TG2-targeted antibodies produced by recombination technology inhibited endothelial tubule formation and enhanced the crosslinking activity of TG2. When this enzymatic activity was inhibited using site-directed irreversible TG2 inhibitors in the presence of autoantibodies, in vitro angiogenesis reverted to the control level. Conclusions. Since we found a significant negative correlation between endothelial cell angiogenesis and TG2 activity, we suggest that the anti-angiogenic effects of coeliac patient-derived TG2-targeted autoantibodies are exerted by enhanced enzymatic activity of TG2.

Celiac disease IgA modulates vascular permeability in vitro through the activity of transglutaminase 2 and RhoA

Celiac disease is characterized by the presence of specific autoantibodies targeted against transglutaminase 2 (TG2) in untreated patients' serum and at their production site in the small-bowel mucosa below the basement membrane and around the blood vessels. As these autoantibodies have biological activity in vitro, such as inhibition of angiogenesis, we studied if they might also modulate the endothelial barrier function. Our results show that celiac disease patient autoantibodies increase endothelial permeability for macromolecules, and enhance the binding of lymphocytes to the endothelium and their transendothelial migration when compared to control antibodies in an endothelial cell-based in vitro model. We also demonstrate that these effects are mediated by increased activities of TG2 and RhoA. Since the small bowel mucosal endothelium serves as a "gatekeeper" in inflammatory processes, the disease-specific autoantibodies targeted against TG2 could thus contribute to the pathogenic cascade of celiac disease by increasing blood vessel permeability.