948 resultados para Generational Turnover


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Mit dem Ende des 18. Parteitags der Kommunistischen Partei Chinas (KPCh) Mitte November kam auch die erste Phase des chinesische Führungswechsels zum Abschluss. Die wichtigste Aufgabe der Partei während dieser Phase war es, den ständigen Ausschuss des Politbüros, also das oberste beschlussfassende Organ der Partei, zu wählen. Zusätzlich zur Ernennung von sieben Spitzenpolitikern umfasst der Wechsel auch die Wahl eines neuen Politbüros und des Zentralkomitees als Vertretung der 83 Millionen Parteimitglieder.

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Assessment of protein dynamics in living cells is crucial for understanding their biological properties and functions. The SNAP-tag, a self labeling suicide enzyme, presents a tool with unique features that can be adopted for determining protein dynamics in living cells. Here we present detailed protocols for the use of SNAP in fluorescent pulse-chase and quench-chase-pulse experiments. These time-slicing methods provide powerful tools to assay and quantify the fate and turnover rate of proteins of different ages. We cover advantages and pitfalls of SNAP-tagging in fixed- and live-cell studies and evaluate the recently developed fast-acting SNAPf variant. In addition, to facilitate the analysis of protein turnover datasets, we present an automated algorithm for spot recognition and quantification.

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Includes bibliographical references.

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WI docs no.: EMP 1.1/2:4/1963-1971

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"Prepared by Boris Emmet"--p. 5.

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Research conducted by LaSalle Governmental Consultants, Ltd.

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Item 1005-C

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Mode of access: Internet.

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Prepared for the Employment and Training Administration, U.S. Department of Labor, under Research and Development Grant No. 91-48-78-39.

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Mode of access: Internet.

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Bibliography: leaf 25.

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Thesis (Ph.D.)--University of Washington, 2016-06