Phospholipase C-linked receptors regulate the ATP-sensitive potassium channel by means of phosphatidylinositol 4,5-bisphosphate metabolism

In the COS7 cells transfected with cDNAs of the Kir6.2, SUR2A, and M1 muscarinic receptors, we activated the ATP-sensitive potassium (KATP) channel with a K+ channel opener and recorded the whole-cell KATP current. The KATP current was reversibly inhibited by the stimulation of the M1 receptor, which is linked to phospholipase C (PLC) by the Gq protein. The receptor-mediated inhibition was observed even when protein kinase C (PKC) was inhibited by H-7 or by chelating intracellular Ca2+ with 10 mM 1,2-bis(2-aminophenoxy)ethane-N,N,N′,N′-tetraacetate (BAPTA) included in the pipette solution. However, the receptor-mediated inhibition was blocked by U-73122, a PLC inhibitor. M1-receptor stimulation failed to inhibit the KATP current activated by the injection of exogenous phosphatidylinositol 4,5-bisphosphate (PIP2) through the whole-cell patch pipette. The receptor-mediated inhibition became irreversible when the replenishment of PIP2 was blocked by wortmannin (an inhibitor of phosphatidylinositol kinases), or by including adenosine 5′-[β,γ–imido]triphosphate (AMPPNP, a nonhydrolyzable ATP analogue) in the pipette solution. In inside-out patch experiments, the ATP sensitivity of the KATP channel was significantly higher when the M1 receptor in the patch membrane was stimulated by acetylcholine. The stimulatory effect of pinacidil was also attenuated under this condition. We postulate that stimulation of PLC-linked receptors inhibited the KATP channel by increasing the ATP sensitivity, not through PKC activation, but most probably through changing PIP2 levels.

Retrieval of phenological stages of onion fields during the first year of growth by means of C-band polarimetric SAR measurements

The phenological stages of onion fields in the first year of growth are estimated using polarimetric observables and single-polarization intensity channels. Experiments are undertaken on a time series of RADARSAT-2 C-band full-polarimetric synthetic aperture radar (SAR) images collected in 2009 over the Barrax region, Spain, where ground truth information about onion growth stages is provided by the European Space Agency (ESA)-funded agricultural bio/geophysical retrieval from frequent repeat pass SAR and optical imaging (AgriSAR) field campaign conducted in that area. The experimental results demonstrate that polarimetric entropy or copolar coherence when used jointly with the cross-polarized intensity allows unambiguously distinguishing three phenological intervals.

Experimental researches concerning the philosophy of permanent colours; and the best means of producing them, by dyeing, calico printing, &c.

Includes index.

The medical companion, or family physician; treating of the diseases of the United States, with their symptoms, causes, cure and means of prevention: common cases in surgery, as fractures, dislocations, &c. the management and diseases of women and children. A dispensatory, for preparing family medicine, and a glossary explaining technical terms. To which are added, a brief anatomy and physiology of the human body, shewing, on rational principles, the cause and cure of diseases: an essay on hygiene, or the art of preserving health, without the aid of medicine: an American materia medica, pointing out the virtues and doses of our medicinal plants. Also, the nurse's guide,

Mode of access: Internet.

The farmer's letters to the people of England : containing the sentiments of a practical husbandman, on various subjects of great importance, particularly the exportation of corn, the balance of agriculture and manufactures, the present state of husbandry, the circumstances attending large and small farms, the present state of the poor, the price of provisions, the proceedings of the Society for the Encouragement of Arts, &c., the importance of timber and planting, emigrations to the colonies, the means of promoting the agriculture and population of Great Britain, &c. &c. : to which are added, Sylvæ, or, Occasional tracts on husbandry and rural œconomics ... : in two volumes.

Edition statement preceedes the words: in two volumes.

The history and proceedings of the House of Commons from the Restoration to the present time. Containing the most remarkable motions, speeches, resolves, reports and conferences to be met with in that interval: as also the most exact estimates of the charge of government, state of the public revenue, the rise and growth of the national debt, expence of the war, proceedings on ways and means, speeches and messages from the throne, addresses, and remonstrances, also the numbers pro and con upon every division &c. Many of which curious particulars were never before printed. Collected from the best authorities, compared with the journals of the House, and illustrated with a great variety of historical and explanatory notes. Together with a large appendix, containing exact lists of every Parliament, the names of the speakers, their several posts under the government, and other valuable, supplemental pieces.

Includes indexes.

The history of Mexico, from the Spanish conquest to the present era; containing a condensed and connected general view of the manners, customs, religion, commerce, soil, and agriculture - animal, vegetable, and mineral productions - a concise political and statistical review of the changes effected in that country, with is present form of government, &c. &c. - Also, observations, speculative and practical, as to the best means of working the Mexican mines, by a combination of British talent, capital, and machinery.

Mode of access: Internet.

Colonial slavery. Letters to the Right Hon. William Huskisson, President of the Board of Trade, &c., &c. on the present condition of the slaves, and the means best adapted to promote the mitigation and final extinction of slavery in the British colonies.

Mode of access: Internet.

Consumo c(l)iente: abordagem do gênero anúncio publicitário em livros didáticos de língua portuguesa do ensino médio

Portuguese language textbooks, according to what has been preconized on the official document to education, have been configured on discursive genres imported from diverse spheres of human activity. Adverts, genre of ample social circulation, spread from the Advertising sphere to the schools and started being approached by these collectaneas as an object and a tool for teaching. Therefore, this research deals with the approach of ads in Portugese textbooks. These discursive practices matter for the impact or appeal they exert over the (new) consumers, among which High School students; for their representation in the capitalist system, which guides us on our relationships and social practices; and for the mix of languages that end up at their composition, once they encapsulate the spirit of our time, par excellence, the one from the verbal-visual genres. To understand the treatment given to these advertising pieces, from questions/commentaries related to them, two collections were selected by the Programa Nacional do Livro Didático – Textbook National Program (PNLD 2012) among the ones more used by public High Schools in Natal/RN. From Applied Linguistics, from mestizo, nomadic and inter/transdisciplinary identity (MOITA LOPES, 2009), this study falls within the discursive chain of the interpretive tradition of historical-cultural approach (FREITAS, 2010) and names the Bakhtin Circle and its language‟s dialogical conception as inescapable partners. The data of the colletaneas show that the genre approach can happen as concrete utterance, as linguistic artifact and as hybrid, at work with questions and without questions, with the predominance of its occurrence in the portion of the volume devoted to the study of grammar. In the literature chapters and production/interpretation of compositions, it insert is incipient or it doesn‟t happen in the volume. Such a provision has implications for multiliteracies (ROJO, 2012) of the citizen student, once the lack or the abundance of critical reading proposals for this genre, that demand from the student the exercise of knowledge that is necessary to the construction of linguistic and social meanings, can be responsible for guide to a more conscious consumerism (material and cultural) by the chief customers of the work under review. The approaches of the genres seems to indicate a gradual transition that such material have undergone, which means, from the focus on clauses to the focus on utterances, or even the approach as linguistic artifact to hybrid and the concrete utterance, in search of overcoming the traditional tendency of taking advantage of formal aspects of the language, to the detriment of enunciative ones, and for coming into harmony with the guidelines and parameters of teaching in contemporary times, bringing the school duties close to the rights in life.

Partner selection for reverse logistics centres in green supply chains: a fuzzy artificial immune optimization approach

The design of reverse logistics networks has now emerged as a major issue for manufacturers, not only in developed countries where legislation and societal pressures are strong, but also in developing countries where the adoption of reverse logistics practices may offer a competitive advantage. This paper presents a new model for partner selection for reverse logistic centres in green supply chains. The model offers three advantages. Firstly, it enables economic, environment, and social factors to be considered simultaneously. Secondly, by integrating fuzzy set theory and artificial immune optimization technology, it enables both quantitative and qualitative criteria to be considered simultaneously throughout the whole decision-making process. Thirdly, it extends the flat criteria structure for partner selection evaluation for reverse logistics centres to the more suitable hierarchy structure. The applicability of the model is demonstrated by means of an empirical application based on data from a Chinese electronic equipment and instruments manufacturing company.

Utilização do “cheque-dentista” por parte de crianças de 10 e 13 anos no ano letivo 2013/2014 em duas escolas no agrupamento de Vizela

Introdução: Uma grande parte de todas as consultas de medicina dentária realizadas em Portugal ocorre em prestadores de natureza privada, consequentemente a acessibilidade, principalmente entre os estatutos socioeconomicamente mais desfavorecido é dificultada. As crianças e os jovens são um grupo especial da população que necessita de particular atenção e proteção por parte dos serviços governamentais, investir na sua saúde e no seu bem‐estar garante ganhos de saúde ao longo das suas vidas. Tendo isto em conta, foi criado o Programa Nacional de Promoção de Saúde Oral (PNPSO). Os objetivos principais deste programa consistem na redução da incidência de doenças orais, melhoria dos conhecimentos e comportamentos sobre saúde oral e a promoção da equidade na prestação de cuidados de saúde oral. Desta forma são emitidos cheques-dentista para determinados grupos populacionais, sendo eles crianças e jovens com idade inferior a 16 anos, gravidas a ser seguidas no SNS, beneficiários do complemento solidário para idosos, portadores de Sida/VIH, e consultas no âmbito da prevenção do cancro oral. Participantes e Métodos: Realizou-se um estudo observacional transversal onde a população em análise foi constituída pelos responsáveis dos alunos de 10 e 13 anos abrangidos pelo PNPSO que no ano letivo 2013/2014 frequentaram o Colégio de Vizela e o Instituto Silva Monteiro. A recolha de dados foi feita através de um inquérito realizado por escrito com questões relativas à utilização dos documentos no âmbito do PNPSO. Em ambas as situações esteve presente o consentimento informado e garantiu-se a total confidencialidade dos dados. Os dados recolhidos neste estudo foram submetidos a uma análise estatística recorrendo ao software IBM SPSS Statistics v22. Resultados: Na população analisada quando questionados “O seu educando já tinha tido alguma consulta de medicina dentária?” 88,5% responderam “sim”, desses a maioria referiu que o médico dentista onde essas consultas foram realizadas estava incluído no programa (81,5%). Uma grande parte dos inquiridos referiu a escola como fator que lhes deu a conhecer o programa (sendo que 90,2% incluíram essa opção nas suas respostas). Quando questionados se fizeram tratamentos fora do programa 54,9% responderam que não. Em relação à utilização do(s) cheque(s)-dentista a que tiveram direito, 86,1% dos beneficiários referiu ter utilizado, desses, 67,6% mencionou a conclusão dos tratamentos com as consultas no âmbito do programa. Quando questionados o que os levou a escolher o consultório onde os tratamentos incluídos no PNPSO foram realizados, 57,9% do total de respostas foram para o “conhecimento prévio do médico dentista”. Na opinião de grande parte dos inquiridos (97,5%), o cheque-dentista é um incentivo para cuidados de saúde oral. No futuro, 99,2% dos beneficiários referiram que irão realizar os tratamentos a que tenham direito com o PNPSO. Conclusão: Com este estudo foi possível observar que grande parte dos beneficiários analisados utilizou o(s) cheque(s)-dentista a que tiveram direito. É possível observar que a maioria dos utentes referiram ter beneficiado com o programa, e afirmam que este constitui um meio de promoção e prevenção de doenças orais futuras e um incentivo para os cuidados de saúde oral. O processo de divulgação do PNPSO foi na sua maioria realizado pelas escolas, em que ambas se revelaram competentes a dar a conhecer o programa aos beneficiários.

Diagnóstico automático de eventos e distúrbios em unidades geradoras e usinas utilizando lógica fuzzy

The electric power systems are getting more complex and covering larger areas day by day. This fact has been contribuiting to the development of monitoring techniques that aim to help the analysis, control and planning of power systems. Supervisory Control and Data Acquisition (SCADA) systems, Wide Area Measurement Systems and disturbance record systems. Unlike SCADA and WAMS, disturbance record systems are mainly used for offilne analysis in occurrences where a fault resulted in tripping of and apparatus such as a transimission line, transformer, generator and so on. The device responsible for record the disturbances is called Digital Fault Recorder (DFR) and records, basically, electrical quantities as voltage and currents and also, records digital information from protection system devices. Generally, in power plants, all the DFRs data are centralized in the utility data centre and it results in an excess of data that difficults the task of analysis by the specialist engineers. This dissertation shows a new methodology for automated analysis of disturbances in power plants. A fuzzy reasoning system is proposed to deal with the data from the DFRs. The objective of the system is to help the engineer resposnible for the analysis of the DFRs’s information by means of a pre-classification of data. For that, the fuzzy system is responsible for generating unit operational state diagnosis and fault classification.

Contributos das estruturas de orientação educativa na gestão escolar: Estudo de caso no agrupamento de escolas nº 4 de Évora

A presente investigação iniciou-se a partir da identificação do problema/ questão de partida: Quais os contributos das estruturas de orientação educativa para a gestão e administração escolar? Esta questão foi o fio condutor da presente investigação, "Contributos das Estruturas de Orientação Educativa na Gestão e Administração Escolar - Estudo de Caso no Agrupamento de Escolas nº 4 de Évora". Neste sentido, constituíram-se como objectivos específicos da presente investigação os seguintes: • Avaliar o funcionamento das estruturas de orientação educativa do Agrupamento de Escolas n.º 4 de Évora. • Identificar os pontos fortes e fracos da intervenção das estruturas de orientação na gestão e administração do Agrupamento de Escolas n.º 4 de Évora. • Elaborar um plano de acção com vista à optimização das estruturas de orientação educativa. Em relação ao estudo empírico, e como forma de dar resposta às questões da investigação, optámos por uma metodologia mista, quantitativa e qualitativa. Após a apresentação dos dados recolhidos, da sua análise e respectiva interpretação, apurámos as seguintes conclusões: A maioria dos inquiridos, neste caso, os docentes, têm opinião favorável em relação às diferentes estruturas, ressalva-se pela negativa a Associação de Pais/Encarregados de Educação, devido a estar inactiva e, pela positiva o Conselho Executivo, consideram-no como um órgão dinamizador de todas as estruturas do Agrupamento; a maioria dos não docentes tem boa opinião sobre o desempenho das suas funções e revela a importância da sua representação no Conselho Pedagógico e na Assembleia de Escola. Os pais e encarregados de educação inquiridos, são consensuais em relação à colaboração com os professores, principalmente com o Director de Turma. Os Directores de Turma inquiridos referem a colaboração com os pais e encarregados de educação, e realçam o papel do órgão de gestão como dinamizador da Associação de Pais/Encarregados de Educação. Os membros da Assembleia de Escola inquiridos, consideram o seu desempenho como activo e dinamizador no processo de aplicação do novo regime de autonomia. No final da nossa dissertação, expressámos as recomendações, acções e sugestões que possam potenciar futuras investigações. ABSTRACT; The present investigation started from the identification of the problem/ beginning question: What are the contributions of the structures of educational guidance for the management and school administration? This question was the guide line of the research, "Contributions of the Structures of Educational Guidance for the Management and School Administration- Exploratory study in the 4th Group of Schools of Évora". ln this sense, the specific objectives of this investigation are the following: • Evaluate the function of the structures of educational guidance of the 4th Group of Évora. • ldentify the strengths and weaknesses of the intervention of structures of educational guidance in the management and administration of the 4th Group of Évora. • Elaborate an action plan to optimize the structures of educational guidance. For the empirical study and in order to address the question of the investigation, was chosen a mixed methodology, quantitative and qualitative. After submission of the collected data, of their analysis and their interpretation, we established the following conclusions: Most of the participants, in this case teachers, have favorable opinion towards the different structures, noted as a negative feature is the Parents Association, due to be inactive, and the Executive Board as positive, because it is viewed as a promoter of all the structures of the Group; most of the non-teaching staff have a good opinion about the performance of its functions and shows the importance of their representation on the Executive Board and in the School Assembly. The parents who participate in the inquiry are in agreement with the teachers regarding the corporation between them, particularly with the Class Director. The Class Directors respondents mention the cooperation with the parents and enhance the role of the Executive Board as a promoter of the Parents Association. The members of the School Assembly respondents consider their performance as active and dynamic in the application process of the new system of autonomy. In the end of this thesis we express our recommendations, actions and suggestions that may promote future research.