Geoprópolis de Melipona fasciculata SMITH: ações citotóxica, imunomoduladora, antibacteriana e antifúngica

Pós-graduação em Patologia - FMB

Pesquisa de fatores de patogenicidade em Escherichia coli Enteroagregativas da microbiota intestinal de pacientes com doença inflamatória intestinal

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Trabalhadores nos canteiros de obras da UHE Belo Monte-Altamira: condições de saúde e políticas públicas

A construção da Usina Hidroelétrica (UHE) de Belo Monte no rio Xingu na região de Altamira no Estado do Pará, na Amazônia, tem sido alvo de grandes polêmicas e debates em diversos espaços sociais, institucionais e na mídia, devido à complexidade dos impactos que a obra traz à sociedade, ao ambiente, aos povos indígenas, pescadores e ribeirinhos, resultando em diversas ações judiciais, mas também em greves e reivindicação dos trabalhadores por melhorias salariais, condições de trabalho e saúde. O estudo busca, por meio de uma abordagem qualitativa e descritiva, primeiro, analisar o quadro de adoecimento entre os operários contratados e, segundo, caracterizar as formas de atendimento à saúde na cidade de Altamira, exame que está enquadrado na incapacidade das políticas públicas de responder às demandas e produzir a adequação mínima ao aumento do fluxo migratório provocado pelo megaempreendimento. Durante o trabalho de campo, no período de 09 a 24 de julho de 2013, foram entrevistados 25 trabalhadores dos canteiros de obras da UHE, utilizando formulário com perguntas abertas e fechadas. O levantamento de fontes privilegiou pesquisa nos jornais e Instituições públicas e privadas acerca da problemática relacionada às condições de trabalho e saúde dos trabalhadores dos canteiros de obras da UHE Belo Monte, a fim de possibilitar um esquema interpretativo que ainda pudesse indicar proposições no sentido da prevenção de agravos no campo da saúde dos trabalhadores.

RT-PCR for confirmation of echovirus 30 isolated in Belém, Brazil

Echovirus (Echo) 30 or human enterovirus B is the most frequent enterovirus associated with meningitis cases. Epidemics and outbreaks of this disease caused by Echo 30 have occurred in several countries. In Brazil, Echo 30 has been isolated from sporadic cases and outbreaks that occurred mainly in the south and southeast regions. We used RT-PCR to examine Echo 30 isolates from meningitis cases detected from March 2002 to December 2003 in Belém, state of Pará, in northern Brazil. The patients were attended in a Basic Health Unit (State Health Secretary of Pará), where cerebrospinal fluid (CSF) was collected and stored in liquid nitrogen. Weekly visits were made by technicians from Evandro Chagas Institute to the health unit and samples were stored at -70ºC in the laboratory until use. HEp-2 and RD cell lines were used for viral isolation and neutralization with specific antisera for viral identification. RNA extraction was made using Trizol reagent. The RT-PCR was made in one step, and the total mixture (50 µL) was composed of: RNA, reaction buffer, dNTP, primers, Rnase inhibitor, reverse transcriptase, Taq polymerase and water. The products were visualized in agarose gel stained with ethidium bromide, visualized under UV light. Among the 279 CSF samples examined, 30 (10.7%) were EV positive, 29 being Echo 30 and one was Cox B. Nineteen Echo 30 were examined with RT-PCR; 18 tested positive (762 and 494 base pairs). The use of this technique permitted viral identification in less time than usual, which benefits the patient and is of importance for public-health interventions.

Análise da expressão gênica em resposta ao tratamento com Euphorbia tirucalli (Aveloz) em carcinoma epidermóide de laringe

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Inflammation and cancer: role of Annexin A1 and FPR2/ALX in proliferation and metastasis in human laryngeal squamous cell carcinoma

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Heparin and dextran sulfate: their role on rsv infectivity

Respiratory syncytial virus is the major cause of acute lower respiratory tract illness in infants and young children. Because there is currently no licensed vaccine for RSV, there is a substantial interest in the identification and development of RSV specific inhibitory agents. There are clinical evidences that glycosaminoglycans (GAGs) are potential inhibitors of viral infection. In this study, the performance of two GAGs (heparin and dextran sulfate) were compared for their antiviral and virucidal activities on RSV. Analysis was performed using an in vitro infection model where, previously to infection, Hep-2 cells or RSV were incubated with heparin or dextran sulfate. The presence of viral particles was analyzed by Reverse Transcriptase-Polimerase Chain Reaction (RT-PCR) and indirect immunofluorescence assays (IFA). The results showed that viral infection was more efficiently inhibited when Hep-2 cells were pre-incubated with heparin or, when viral particles were pre-incubated with dextran sulfate. Our study suggest that, in the absence of cellular death, heparin and dextran sulfate reduce RSV infection by different mechanisms, antiviral and virucidal ones, respectively. These data contribute for recent medical, microbiology and biochemical studies which suggest that the use of antiviral and virucidal compounds as more effective treatment to control virus infections.

Evaluation of photodynamic therapy in adhesion protein expression

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Ação do extrato de glândulas salivares de fêmeas do carrapato Rhipicephalus sanguineus (Latreille, 1806) sobre a inibição da proliferação de células de linhagem tumoral

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Avaliação dos efeitos antitumorais de extratos brutos produzidos por fungos endofíticos isolados de Eugenia jambolana em células de hepatocarcinoma murino (Hepa-1c1c7)

Pós-graduação em Biociências e Biotecnologia Aplicadas à Farmácia - FCFAR

Phenotypic and genetic features of enteropathogenic Escherichia coli isolates from diarrheal children in the Ribeirao Preto metropolitan area, Sao Paulo State, Brazil

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Escherichia coli from Crohn’s disease patient displays virulence features of enteroinvasive (EIEC), enterohemorragic (EHEC), and enteroaggregative (EAEC) pathotypes

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

The chemical composition and pharmacological activities of geopropolis produced by Melipona fasciculata Smith in northeast Brazil

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

P-220-Escherichia coli displaying virulence features of multiple diarrheogenic pathotypes detected in a Crohn's disease patient

Background: The number of Escherichia coli in the gut of Crohn's disease (CD) patients is higher than that of normal subjects, but the virulence potential of these bacteria is not fully known. Previous studies have shown that these E. coli are closely related to extraintestinal pathogenic categories (ExPEC), are able to invade epithelial cells, and usually do not produce exotoxins. We report here the detection, in a CD patient, of an E. coli which belongs to a classical enteropathogenic (EPEC) serotype and displays virulence markers of enteroinvasive (EIEC), enteroaggregative (EAEC) and enterohemorrhagic (EHEC) pathotypes. Methods: The E. coli strain was isolated, in 2009, by classical bacteriological procedures from a 56 year old woman who underwent ileo-terminal resection 1 year before, due to intestinal obstruction. The bacterial characterization was carried out by in vitro adhesion and invasion assays to cultured epithelial cells and macrophages and screening by PCR to identify virulence genetic markers of diarrheogenic E. coli (DEC) and to detect one of the gene combinations which define the phylogroups of the E. coli reference (EcoR) collection. The strain was also tested for the ability to produce biofilm and shiga cytotoxins and had its whole genome sequenced by Ion Torrent Sequencing Technology. Results: The studied strain, which was detected both in ileum biopsies and the stools of the patient, displayed the aggregative adherence (AA) phenotype to Hep-2 cells and an ability to enter Caco-2 cells 3x as high as that of EIEC reference strain and 89% of that of the prototype AIEC LF82 strain. Although it could invade cultured macrophages, the strain was unable to replicate inside these cells. PCR screening revealed the presence of eae, aggR and stx1. Tests with bacterial culture supernatants in Vero cells demonstrating cytotoxicity suggested the production of Stx1. In addition, the strain revealed to be a strong biofilm producer, belonged to the B2 EcoR phylogroup, to the O126:H27 serogroup and to the multilocus sequencing type (MLST) ST3057. The 2 later features were deduced from the whole genome sequence of the strain. Conclusions: The characterization of this E. coli isolate from a CD patient revealed a combination of virulence markers of distinct DEC pathotypes, namely eae and stx1 of EHEC, AA, aggR and biofilm formation of EAEC, and invasiveness of EIEC. These features along with its serotype and phylogroup identity seem to suggest a potential to be involved in CD, an observation which should be tested with additional studies.

Capacidade de adesão, invasão e produção de enterotoxinas de Staphylococcus aureus isolados de leite de vacas com mastite subclínica e leite humano de um banco de leite

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)