Analysis of Protein Turnover by Quantitative SNAP-Based Pulse-Chase Imaging

Assessment of protein dynamics in living cells is crucial for understanding their biological properties and functions. The SNAP-tag, a self labeling suicide enzyme, presents a tool with unique features that can be adopted for determining protein dynamics in living cells. Here we present detailed protocols for the use of SNAP in fluorescent pulse-chase and quench-chase-pulse experiments. These time-slicing methods provide powerful tools to assay and quantify the fate and turnover rate of proteins of different ages. We cover advantages and pitfalls of SNAP-tagging in fixed- and live-cell studies and evaluate the recently developed fast-acting SNAPf variant. In addition, to facilitate the analysis of protein turnover datasets, we present an automated algorithm for spot recognition and quantification.

Labor turnover : calculation and cost.

Includes bibliographical references.

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"B-249785"--P. [1].

Wisconsin employment, earnings, and labor turnover.

WI docs no.: EMP 1.1/2:4/1963-1971

The turnover of labor : November, 1919.

"Prepared by Boris Emmet"--p. 5.

Turnover analysis manual /

Research conducted by LaSalle Governmental Consultants, Ltd.

Employee turnover in the federal government.

Item 1005-C

The measurement and significance of labor turnover /

Mode of access: Internet.

The structure of withdrawal : relationships among estrangement, tardiness, absenteeism, and turnover /

Prepared for the Employment and Training Administration, U.S. Department of Labor, under Research and Development Grant No. 91-48-78-39.

Employee turnover in the city service of Philadelphia

Mode of access: Internet.

Performance of Arkansas workers : metalworking plants ; productivity, trainability, turnover and absenteeism work contingencies. /

Mode of access: Internet.

The unemployment insurance tax and labor turnover : an empirical analysis /

Bibliography: leaf 25.

The rise of modern mammalian faunas: tempo and mode of faunal turnover in western Montana during the Oligocene

Thesis (Ph.D.)--University of Washington, 2016-06