Caution for using ventilatory frequency as an indicator of stress in fish

This study tested the use of ventilatory frequency (VF) as an indicator of stress in the Nile tilapia, Oreochromis niloticus (L.). Firstly, we tested the relationship between VF and plasma cortisol after confinement. Confined fish showed higher VF and plasma cortisol levels, but the latter continued to increase significantly for longer time than VF. Secondly, we conducted another experiment to test the use of VF as indicator of fish stress. In four out of six treatment, we confined the fish for different intervals (30 s, 5, 15 or 30 min). The others were used as control. In one, no handling was imposed. The other control consisted of introducing the partition (the same used to perform the confinement) into the aquarium for less than 4 s, without confinement and immediately removing the partition (partition control). Ventilatory frequency was increased for the partition control as much as for the longer duration of confinement. This clearly indicates that VF is a very sensitivity response to disturbance, but of limited use because this parameter does not reflect the severity of the stimulus. Thus, although VF is a non-invasive technique that does not require sophisticated recording equipment, its usefulness is limited. (C) 2004 Elsevier B.V. All rights reserved.

Eye color as an indicator of social rank in the fish Nile tilapia

We investigated the association of eye color with the dominant-subordinate relationship in the fish Nile tilapia, Oreochromis niloticus. Eye color pattern was also examined in relation to the intensity of attacks. We paired 20 size-matched fish (intruder: 73.69 ± 11.49 g; resident: 75.42 ± 8.83 g) and evaluated eye color and fights. These fish were isolated in individual aquaria for 10 days and then their eye color was measured 5 min before pairing (basal values). Twenty minutes after pairing, eye color and fights were quantified for 10 min. Clear establishment of social hierarchy was observed in 7 of 10 pairs of fish. Number of attacks ranged from 1 to 168 among pairs. The quartile was calculated for these data and the pairs were then divided into two classes: low-attack (1 to 111 attacks - 2 lower quartiles) or high-attack (112 to 168 attacks - 2 higher quartiles). Dominance decreased the eye-darkening patterns of the fish after pairing, while subordinance increased darkening compared to dominance. Subordinate fish in low-attack confrontations presented a darker eye compared to dominant fish and to the basal condition. We also observed a paler eye pattern in dominants that shared low-attack interactions after pairing compared to the subordinates and within the group. However, we found no differences in the darkening pattern between dominants and subordinates from the high-attack groups. We conclude that eye color is associated with social rank in this species. Moreover, the association between eye color and social rank in the low-attack pairs may function to reduce aggression.

Skin diseases in Guiana dolphins (Sotalia guianensis) from the Paranagua estuary, Brazil: A possible indicator of a compromised marine environment

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Schur-SzegA composition of entire functions

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Water quality index as a simple indicator of aquaculture effects on aquatic bodies

This paper proposes a water quality index (WQI) to subsidize management actions in the Medio Paranapanema Watershed in São Paulo State, Brazil, as a simple pollution indicator for aquaculture activity. Water quality of the Macuco and Queixada rivers was investigated for 2 years (from May 2003 to May 2005). The index proposed in this work is composed of three measurable environmental parameters-turbidity, total phosphorus and dissolved oxygen. Concentrations of these three variables were normalized on a scale from 0 to 100 and translated into statements of water quality (excellent, good, regular, fair and poor). The index was applied to seventeen monitoring points in the aquatic bodies described above and compared to others, one being that used by the Environmental Protection Agency of United States and proposed for the National Sanitation Foundation, other employing minimal index and the last one considering the minimum operator concept. The results show that the degradation in this watershed from aquaculture activity can be easily inferred with this index, which is more restricted than the others routinely used to infer water quality. (C) 2007 Elsevier Ltd. All rights reserved.

On the calculation of inner products of Schur functions

Two methods for calculating inner products of Schur functions in terms of outer products and plethysms are given and they are easy to implement on a machine. One of these is derived from a recent analysis of the SO(8) proton-neutron pairing model of atomic nuclei. The two methods allow for generation of inner products for the Schur functions of degree up to 20 and even beyond.

Chromatic alteration in Arenaeus cribrarius (Lamarck) (Crustacea, Portunidae): an indicator of sexual maturity

Some individuals of the species Arenaeus cribrarius (Lamarck, 1818) bear a characteristic pink abdomen, which is notably different from the usual white coloration. The incidence of this chromatic alteration was determined for a single population and its relation with other individual variables were examined. The individuals were monthly collected from May, 1991 to April, 1993, in Ubatuba, São Paulo, with the aid of a shrimp fishery boat provided with double-rig trawling nets. All specimens were sexed, measured (CW = carapace width), distributed in 10-mm size classes and classified according to growth phase (juvenile and adult), molting condition and development stage of gonads. The occurrence of pink-colored morphs was also recorded. From a total of 2,096 collected individuals, only 60 females (nine of those ovigerous) presented a pink-colored abdomen, which represents 2.9% of the whole sample and 5.2% of the females. Almost all of them were intermolt individuals (96.6%) and 63.3% showed mature gonads. According to published data, size at the onset of functional maturity in A. cribrarius females is around 60 mm CW, from which the incidence of pink morphs and ovigerous crabs were recorded. The obtained results suggest that such a chromatic alteration is associated to sexual maturity in these females. This characteristic may enhance the attraction potential for mating, shortly after the puberty molt.

Use of cationic surfactant-xylenol orange indicator in metals complexometry by back-titration with EDTA.

The interaction of the xilenol orange and commercial disinfectant containing itself cationic-surfactant allowed the use indicator up to pH 11 by back-titration with EDTA, and the determination of total or partial concentration of metallic ions samples using the same indicator at the highest pH range.

A neural approach for determination of global energetic efficiency indicator in groundwater wells

In most of the cases, the systems of water distribution from groundwater wells use electrical submersible pumps. All electrical energy is applied to the pumps; however, other components (pipes, valves, etc.) of these systems are also responsible by the higher or lower consumption of electric energy. The supervisors and operators of the systems should thus have knowledge of the global energetic behavior of the process in order to administrate it properly. This work suggests a 'Global Energetic Efficiency Indicator' for groundwater wells by using mathematical equations and neural networks. Simulation results will be presented in order to demonstrate the validity of the proposed approach.

Short communication: Resazurin reducing time as an indicator of Bradyrhizobium viable cell count

A method based on the use of resazurin (RSZ) is described to determine the number of viable Bradyrhizobium japonicum cells in culture medium. The observation of RSZ reduction can be done spectrophotometrically or visually. B. japonicum strains behaved differently when the reducing time was considered. This methodology can be used to determine the number of viable cells during the liquid culture stage of inoculant production.

Peroxidase enzyme (EC.1.11.1.7) activity as an indicator of water stress in sweet pepper plants

The present work was carried out at the Faculdade de Ciências Agronômicas - UNESP, Botucatu, SP. The purpose of the study was to evaluate the physiological and biochemical behavior of sweet pepper (Capsicum annuum L.) plants under different soil water availability conditions and the efficiency of the peroxidase (EC. 1.11.1.7) activity as an indicator of water stress in plants. Sweet pepper plants were grown for 230 days after transplanting of seedlings. The experiment was arranged in a completely randomized experimental design with 4 treatments, two irrigation managements (50 and 1500 kPa) and two soil surface managements (presence or absence of black polyethylene covering), and six replications. Physiological activities, such as stomatal transpiration and resistance to water vapor diffusion, were evaluated, as well as biochemical activities, such as peroxidase activity and total soluble protein in foliar tissues. It was observed that soil water availability may lead to physiological and biochemical alterations in plants. Successive water stress cycles may promote the development of characteristics responsible for improving the plant tolerance to periods of low water availability. The peroxidase enzyme activity showed to be an efficient indicator of water stress in sweet pepper plants.

Peroxidase activity as an indicator of water stress in sweet pepper plants

The purpose of the study was to evaluate the physiological and biochemical behavior of sweet pepper (Capsicum annuum L.) plants under different soil water availability conditions and the efficiency of the peroxidase (EC. 1.11. 1.7) activity as an indicator of water stress in plants. The experiment was carried out at the Faculdade de Ciências Agronômicas UNESP, Botucatu, SP. Sweet pepper plants were grown for 230 days after transplanting of seedlings and arranged in a completely randomized experimental design with 4 treatments, two irrigation managements (50 and 1500 kPa) and two soil surface managements (presence or absence of black polyethylene covering), and six replications. Physiological activities, such as stomatal transpiration and resistance to water vapor diffusion, were evaluated as well as biochemical activities, such as peroxidase activity and total soluble protein in foliar tissues. It was observed that soil water availability may lead to physiological and biochemical alterations in plants. Successive water stress cycles may promote the development of characteristics responsible for improving plant tolerance to periods of low water availability. The peroxidase enzyme activity showed to be an efficient indicator of water stress in sweet pepper plants.

Dental age as indicator of adolescence

Aim: The purpose of the present study was to analyze the relationship between root formation of the first premolars and skeletal maturation stages identified in hand-wrist radiographs. Methods: A cross-sectional study was carried out involving the panoramic and hand-wrist radiographs obtained on the same date of 232 patients, 123 boys and 109 girls aged 4 years and 5 months to 17 years and 12 months. Root formation stages of the first premolars were related to the ossification stages of the sesamoid bone, epiphyseal stages of the phalanx of the thumb and epiphyseal stages of the radius. Results: The studied variables demonstrated statistically significant correlations. Conclusion: Roots of the lower first premolars do not reach 2/3 of their complete length before adolescence.

Genetic (co)variance components for ratio of lamb weight to ewe metabolic weight as an indicator of ewe efficiency

Data from a multibreed commercial flock located at Mid-West of Brazil, supported by Programa de Melhoramento Genético de Caprinos e Ovinos de Corte (GENECOC), were used to estimate genetic parameters of traits related to ewe productivity by Average Information Restricted Maximum Likelihood method applied to an animal model. The analyzed traits were litter weight at birth (LWB) and at weaning (LWW), ewe weight at weaning (EW) and ewe production efficiency, estimated by WEE=LWW/EW 0.75. The heritabilities were 0.26±0.05, 0.32±0.06, 0.37±0.03 and 0.10±0.02 for LWB, LWW, EW and WEE, respectively. Significant effects for direct heterosis were observed for LWW and EW. Recombination losses were important for EW and WEE. Genetic correlations of LWB with LWW, EW and WEE were 0.68, 0.37 and 0.15, respectively; of LWW with EW and WEE were 0.30 and 0.34, respectively; and between EW and WEE was -0.25. Even though it is a low heritability trait, WEE can be indicated as a selection criteria for improving the ewe productivity without increasing the mature weight of animals due to its genetic correlations with LWW and other traits. © 2011 Elsevier B.V.