Richard Baxter Foster Letter to his wife May 24, 1865

Letter from Richard Baxter Foster to his wife Lucy from Brazos Santiago, Texas on May 24, 1865

Richard Baxter Foster Letter to his wife September 1 1865

Letter from Richard Baxter Foster to his wife Lucy from Brownsville, Texas About Sept. 1 1865

Richard Baxter Foster Letter to his wife March 7 1863

Letter from Richard Baxter Foster to his wife Lucy on March 7, 1863

Richard Baxter Foster Letter to his wife July 6 1865

Letter from Richard Baxter Foster to his wife Lucy from Brownsville, Texas on July 6, 1865

Richard Baxter Foster Letter to his wife July 8 1865

Letter from Richard Baxter Foster to his wife Lucy from Brownsville, Texas on July 8, 1865

Richard Baxter Foster Letter to his wife October 17 1865

Letter from Richard Baxter Foster to his wife Lucy on October 17, 1865 at Texas En route to Ft. McIntosh

Richard Baxter Foster Letter to his wife June 2 1865

Letter from Richard Baxter Foster to his wife Lucy from Brownsville, Texas on June 2, 1865

Richard Baxter Foster Letter to his wife May 28 1864

Letter from Richard Baxter Foster to his wife Lucy from Baton Rouge, LA on May 28,1864

Richard Baxter Foster Letter to his wife March 8 1865

Letter from Richard Baxter Foster to his wife Lucy from Brazos Santiago, Texas on March 8, 1865

Richard Baxter Foster Letter to his wife March 30, 1865

Letter from Richard Baxter Foster to his wife Lucy from Brazos Santiago, Texas on March 30, 1865

A comunicação e o papel do comunicador nas ONGs sociais

Estudo sobre a comunicação em três organizações não governamentais de Curitiba-PR que se originaram em movimentos sociais, fundadas nas décadas de 1970, 1980 e 1990. Os objetivos da pesquisa compreenderam encontrar técnicas de comunicação utilizadas para se relacionarem com seus públicos e o papel desempenhado pelo comunicador nessas organizações. A metodologia empregada valeu-se de estudo de casos múltiplos, que incluiu como técnicas de busca de dados a pesquisa documental, bibliográfica e a realização entrevistas com líderes, fundadores e comunicadores das organizações estudadas. Como marco teórico buscou-se nos estudos de comunicação popular alternativa o embasamento para situar as análises realizadas. Dentre os resultados alcançados descobriu-se que, timidamente, há um avanço para o uso de técnicas de comunicação profissionalizadas e uma busca por um profissional que detenha a formação técnica, mas que antes de tudo esteja engajado, comprometido com as causas da organização.(AU)

A comunicação e o papel do comunicador nas ONGs sociais

Estudo sobre a comunicação em três organizações não governamentais de Curitiba-PR que se originaram em movimentos sociais, fundadas nas décadas de 1970, 1980 e 1990. Os objetivos da pesquisa compreenderam encontrar técnicas de comunicação utilizadas para se relacionarem com seus públicos e o papel desempenhado pelo comunicador nessas organizações. A metodologia empregada valeu-se de estudo de casos múltiplos, que incluiu como técnicas de busca de dados a pesquisa documental, bibliográfica e a realização entrevistas com líderes, fundadores e comunicadores das organizações estudadas. Como marco teórico buscou-se nos estudos de comunicação popular alternativa o embasamento para situar as análises realizadas. Dentre os resultados alcançados descobriu-se que, timidamente, há um avanço para o uso de técnicas de comunicação profissionalizadas e uma busca por um profissional que detenha a formação técnica, mas que antes de tudo esteja engajado, comprometido com as causas da organização.(AU)

A complete genome screen for genes predisposing to severe bipolar disorder in two Costa Rican pedigrees

Bipolar mood disorder (BP) is a debilitating syndrome characterized by episodes of mania and depression. We designed a multistage study to detect all major loci predisposing to severe BP (termed BP-I) in two pedigrees drawn from the Central Valley of Costa Rica, where the population is largely descended from a few founders in the 16th–18th centuries. We considered only individuals with BP-I as affected and screened the genome for linkage with 473 microsatellite markers. We used a model for linkage analysis that incorporated a high phenocopy rate and a conservative estimate of penetrance. Our goal in this study was not to establish definitive linkage but rather to detect all regions possibly harboring major genes for BP-I in these pedigrees. To facilitate this aim, we evaluated the degree to which markers that were informative in our data set provided coverage of each genome region; we estimate that at least 94% of the genome has been covered, at a predesignated threshold determined through prior linkage simulation analyses. We report here the results of our genome screen for BP-I loci and indicate several regions that merit further study, including segments in 18q, 18p, and 11p, in which suggestive lod scores were observed for two or more contiguous markers. Isolated lod scores that exceeded our thresholds in one or both families also occurred on chromosomes 1, 2, 3, 4, 5, 7, 13, 15, 16, and 17. Interesting regions highlighted in this genome screen will be followed up using linkage disequilibrium (LD) methods.

Establishment of medaka (Oryzias latipes) transgenic lines with the expression of green fluorescent protein fluorescence exclusively in germ cells: A useful model to monitor germ cells in a live vertebrate

We have generated transgenic medaka (teleost, Oryzias latipes), which allow us to monitor germ cells by green fluorescent protein (GFP) fluorescence in live specimens. Two medaka strains, himedaka (orange–red variety) and inbred QurtE, were used. The transgenic lines were achieved by microinjection of a construct containing the putative promoter region and 3′ region of the medaka vasa gene (olvas). The intensity of GFP fluorescence increases dramatically in primordial germ cells (PGCs) located in the ventrolateral region of the posterior intestine around stage 25 (the onset of blood circulation). Whole-mount in situ hybridization and monitoring of ectopically located cells by GFP fluorescence suggested that (i) the increase in zygotic olvas expression occurs after PGC specification and (ii) PGCs can maintain their cell characteristics ectopically after stages 20–25. Around the day of hatching, the QurtE strain clearly exhibits sexual dimorphisms in the number of GFP fluorescent germ cells, a finding consistent with the appearance of leucophores, a sex-specific marker of QurtE. The GFP expression persists throughout the later stages in the mature ovary and testis. Thus, these transgenic medaka represent a live vertebrate model to investigate how germ cells migrate to form sexually dimorphic gonads, as well as a potential assay system for environmental substances that may affect gonad development. The use of a transgenic construct as a selective marker to efficiently isolate germ-line-transmitting founders during embryogenesis is also discussed.

The optimal measure of allelic association

Allelic association between pairs of loci is derived in terms of the association probability ρ as a function of recombination θ, effective population size N, linear systematic pressure v, and time t, predicting both ρrt, the decrease of association from founders and ρct, the increase by genetic drift, with ρt = ρrt + ρct. These results conform to the Malecot equation, with time replaced by distance on the genetic map, or on the physical map if recombination in the region is uniform. Earlier evidence suggested that ρ is less sensitive to variations in marker allele frequencies than alternative metrics for which there is no probability theory. This robustness is confirmed for six alternatives in eight samples. In none of these 48 tests was the residual variance as small as for ρ. Overall, efficiency was less than 80% for all alternatives, and less than 30% for two of them. Efficiency of alternatives did not increase when information was estimated simultaneously. The swept radius within which substantial values of ρ are conserved lies between 385 and 893 kb, but deviation of parameters between measures is enormously significant. The large effort now being devoted to allelic association has little value unless the ρ metric with the strongest theoretical basis and least sensitivity to marker allele frequencies is used for mapping of marker association and localization of disease loci.