A dominant-negative mutant of human poly(ADP-ribose) polymerase affects cell recovery, apoptosis, and sister chromatid exchange following DNA damage.

Poly(ADP-ribose) polymerase [PARP; NAD+ ADP-ribosyltransferase; NAD+:poly(adenosine-diphosphate-D-ribosyl)-acceptor ADP-D-ribosyltransferase, EC 2.4.2.30] is a zinc-dependent eukaryotic DNA-binding protein that specifically recognizes DNA strand breaks produced by various genotoxic agents. To study the biological function of this enzyme, we have established stable HeLa cell lines that constitutively produce the 46-kDa DNA-binding domain of human PARP (PARP-DBD), leading to the trans-dominant inhibition of resident PARP activity. As a control, a cell line was constructed, producing a point-mutated version of the DBD, which has no affinity for DNA in vitro. Expression of the PARP-DBD had only a slight effect on undamaged cells but had drastic consequences for cells treated with genotoxic agents. Exposure of cell lines expressing the wild-type (wt) or the mutated PARP-DBD, with low doses of N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) resulted in an increase in their doubling time, a G2 + M accumulation, and a marked reduction in cell survival. However, UVC irradiation had no preferential effect on the cell growth or viability of cell lines expressing the PARP-DBD. These PARP-DBD-expressing cells treated with MNNG presented the characteristic nucleosomal DNA ladder, one of the hallmarks of cell death by apoptosis. Moreover, these cells exhibited chromosomal instability as demonstrated by higher frequencies of both spontaneous and MNNG-induced sister chromatid exchanges. Surprisingly, the line producing the mutated DBD had the same behavior as those producing the wt DBD, indicating that the mechanism of action of the dominant-negative mutant involves more than its DNA-binding function. Altogether, these results strongly suggest that PARP is an element of the G2 checkpoint in mammalian cells.

Münşeât-ı Nâbî Efendi : manuscript, 1762

Collection of Nâbî's letters, petitions, and other correspondence to various dignitaries. It was compiled after Nâbî's death by Abdürrahim Çelebi by order of Şehid Ali Paşa.

Suicídio : uma análise epidemiológica : estudo retrospetivo da casuística da Delegação do Sul do Instituto Nacional de Medicina Legal e Ciências Forenses, I.P. (2007-2012)

Trabalho Final do Curso de Mestrado Integrado em Medicina, Faculdade de Medicina, Universidade de Lisboa, 2014

Bologna Process Bucharest Ministerial Conference Takes Place amid Political Crisis in Romania. EUMA Papers Series Vol. 12, No. 5, May 2012

During the last week in April the Ministers responsible for higher education from 47 countries convened in Bucharest, Romania for the Ministerial Conference of the Bologna Process. On April 26 and 27, 2012 the venue for the meeting was the Palace of the Parliament, which was constructed by the dictator Nicolae Ceauşescu in 1984 and completed the year before his death by execution on Christmas Day 1989. One of the largest civilian buildings in the world was location for the first ministerial conference to take place since the European Higher Education Area (EHEA) became effective in 2010. Originally the creation of the EHEA was envisaged by the Bologna Process Declaration in 1999 which had representatives from 29 countries as signatories. This essay will describe the proceedings of the Ministerial Conference, report on the negotiations among delegates in parallel sessions and plenary sessions, discuss the thematic sessions with emphasis on “Global academic mobility: Incentives and barriers, balances and imbalances” and review the adoption of the Bucharest Communiqué and the Bologna Policy Forum Statement.

Factors influencing the experience of the terminal phase and the grieving process: the primary caregiver’s perspective

Theoretical framework: The experience of the terminal phase and death by patients and caregivers is influenced by multiple factors. This study aimed at understanding this experience from the primary caregiver’s perspective. Objectives: To describe the factors that, from the primary caregiver’s perspective, influenced the experience of disease in the terminal phase and mourning for the death of a close person; To understand the influence of nurses’ conduct on the experience of disease in the terminal phase and mourning for the death of a close person from the primary caregiver’s perspective. Methodology: Qualitative descriptive exploratory study. Results: The following factors were valued: To Assume the Caregiver’s Role, To allow for the end-of-life/terminal phase to take place at home/near the family and Process of Care. Regarding the nurses’ conduct, the knowledge, communication and relationship established by nurses were also valued. Conclusion: In addition to expanding the implementation of specific palliative care teams, the acquisition and development of basic skills in this area by most health care professionals is essential.

Sefer ʻEn Yaʻakov.

The last four sedarim of the work were edited after Ibn Habib's death by his son Levi.

The lives of the Lord Chancellors and Keepers of the Great Seal of England.

Vol. 8, edited after the author's death, by Mary Scarlett Campbell, 1869.

Chrestomathie égyptienne, ou Choix de textes égyptiens transcrits, traduits et accompagnés d'un commentaire perpétuel, et précédés d'un abrégé grammatical,

Vols. 3-4 edited after the author's death by his son Vte Jacques de Rougé.

Denkwürdiger und nützlicher rheinischer antiquarius, welcher die wichtigsten und angenehmsten geographischen, historischen und politischen merkwürdigkeiten des ganzen Rheinstroms, von seinem ausflusse in das meer bis zu seinem ursprunge darstellt ; von einem nachforscher in historischen dingen.

The work, which comprised the Mittelrhein only, was continued after the author's death by A.J. Weidenbach, the author of Das Nahethal, II. abth., bd. 16-20 (1869-1871)

English writers: an attempt towards a history of English literature,

Vol. 11 was completed after Morley's death by W. Hall Griffin.

Modern Greek stories,

"Demetra Vaka translated 'The sin of my mother,' 'The god-father' and 'She that was homesick.' The rest were translated by Dr. Aristides E. Phoutrides."

Homērou hapanta, h.e., Homeri opera omnia /

"Plutarchi Vita Homeri et De Homeri poesi incerti auctoris:" v. 5, p. [159]-259.

Opere di monsignor Giovanni della Casa; dopo l'ed. di Fiorenza del MDCCVII e di Venezia del MDCCXXVIII. Molto illustrate e di cose inedite accresciute.

The editing was commenced by Jacopo Carlieri and completed after his death by his son Carlo M. Carlieri.

English writers: an attempt towards a history of English literature,

Vol. 11 was completed after Morley's death by W. Hall Griffin.

Life.

Founded and edited until his death by J. A. Mitchell.