Effect of Lates calcarifer seed stocking on the survival and production of Indian major carps reared in a freshwater coastal pond in Konkan region

The experiment indicated feasibility of culturing Indian major carps seed with Lates calcarifer, if the size of Indian major carps is larger. In the present investigation the average growths of Catla catla, Labeo rohita and Cirrhinus mrigala are recorded as 1193, 1120 and 821 g, respectively, during the year 1997-98, 1998-99 and 1999-2000 in 7.5 months. Complete harvesting of L. calcarifer is essential before stocking of new seed. The average growth of L. calcarifer was recorded as about 670 g during the above period. About 5000 numbers of Indian major carps fry per hectare can be stocked under the present culture system

Effect of size, morphology and crystallinity of seed crystal on the nucleation and growth of single grain Y-Ba-Cu-O

The effect of size, morphology and crystallinity of seed crystals on the nucleation and growth of large grain Y-Ba-Cu-O (YBCO) bulk superconductors fabricated by top seeded melt growth (TSMG) has been investigated. Seeding bulk samples with small, square shaped seed crystals leads to point nucleation and growth of the superconducting YBa2Cu3O7-y (Y-123) phase that exhibits the usual square habitual growth symmetry. The use of triangular and circular shaped seed crystals, however, modifies significantly the growth habit geometry of the grain. The use of large area seeds both increases the rate of epitaxial nucleation of the Y-123 phase and produces relatively large crystals in the incongruent melt, which decreases significantly the processing times of large grain samples. The present study is relevant to decrease processing times of samples with both preferred or no growth sectors and for multiple seeding of large grain samples which contain clean grain boundaries. © 2005 Published by Elsevier Ltd.

The effects of pile flexibility on pile-loading in laterally spreading slopes

Piles passing through laterally spreading slopes can be subjected to considerable loads by the soil flowing past them. Many case histories have been documented of piles which suffered failure as a result of horizontal loads exerted by the flowing soil. This paper details the results of a series of dynamic centrifuge tests carried out at Cambridge University Engineering Department, to investigate the transfer of load from the spreading soil to the piles passing through it, with particular emphasis on the effective stress state of soil elements immediately upslope and downslope of the pile. This soil stress state can be calculated by virtue of instrumentation measuring both horizontal total stress and pore pressures at locations close to the upslope and downslope faces of the piles. By comparison of results obtained for both rigid and flexible piles, conclusions will be drawn as to the effects of pile flexibility on modifying the behavior of the soil-pile system.

Modelling effects of axial load on behaviour of pile groups in laterally-spreading soil

Previous research into the behaviour of piled foundations in laterally-spreading soil deposits has concentrated on pile groups that carry small or negligible axial loads. This paper presents dynamic centrifuge test results for 2 x 2 pile groups with bending and geometric properties similar to real 0.5 m diameter tubular steel and solid circular reinforced-concrete field piles. Axial loads applied represented upper-bounds on typical working loads. The simultaneous scaling of the relevant properties controlling both lateral and axial behaviour allows comparisons to be drawn regarding the particular mechanisms of failure that would dominate for each type of pile. Flexible reinforced-concrete piles which tend to carry lower loads were found to be dominated by lateral effects, while steel piles, which are much stiffer and usually carry greater loads are dominated by settlement considerations. © 2006 Taylor & Francis Group.

Cryopreservation of sperm of common carp, Cyprinus carpio and silver barb, Barbonymus gonionotus for genetically improved seed production

Experiments were conducted to develop and standardize the protocols for cryopreservation of sperm of common carp, Cyprinus carpio and also for using the cryopreserved sperm for fertilization of eggs. Nine extender solutions as Alsever's solution, kurokura-1, kurokura-2, urea egg-yolk, egg-yolk citrate, 0.6% glucose, 0.9% NaCl, Ma and Mb, and five cryoprotectants namely ethanol, methanol, dimethylsulfoxide (DMSO), dimethylamine (DMA) and glycerol were tested. The cryoprotectants were mixed at 10% concentration of the extenders (v/v) to make the cryodiluents. Milt and cryodiluents were mixed at a ratio of 1:9 for Alsever's solution, kurokura-1, kurokura-2, 0.6% glucose and 0.9% NaCl, 1:4 for urea egg-yolk, egg-yolk citrate, Ma and Mb. Among the cryodiluents Alsever's solution mixed with either ethanol or methanol was found to be suitable and it produced more than 90% and 80% spermatozoan motility at equilibrium and post-thaw periods, respectively. Kurokura-1 and kurokura-2 when mixed with the same cryoprotectants showed good spermatozoan motility at equilibrium period (80-90%) but the motility was reduced (30-55%) at post-thaw state. Other extenders did not produce acceptable sperm-motility and in some cases the frozen milt became clotted. Different dilution ratios (1:1, 1:2, 1:4, 1:5, 1:7, 1:9, 1:12, 1:15, 1:20) were formulated for obtaining a suitable milt dilution, the dilution ratio of 1: 9 (milt : cryodiluent) demonstrated the highest post-thaw spermatozoan motility (80%) in Alserver's solution. The optimum concentration of cryoprotectants in the cryodiluents was determined, 10% concentration level was found to be effective to produce the highest number of spermatozoan motility in comparison to the other concentrations (5%, 15%, 20% 30%). Sperm preserved with the cryodiluent Alsever's solution along with either methanol or ethanol was found to be effective to fertilize eggs and produce hatchlings. The hatching rates ranged between 1.48% and 14.76%, compare to control. The fish produced through use of cryopreserved sperm and normal sperm were found to grow well and no significant (P<0.05) growth difference was observed between them. In case of silver barb, Barbonymus gonionotus, sperm tested against six extenders such as egg-yolk citrate, urea-egg-yolk, kurokura-1, kurokura-2, 0.9% NaCl and modified fish ringer (MFR) solution. Cryoprotectants used were the same as those of C. carpio. Milt was diluted with the cryodiluent at a ratio of 1:4 for egg-yolk citrate and urea-egg-yolk, 1:5 for kurokura-1 and 1:9 for 0.9% NaCl, MFR and kurokura-2. The cryoprotectant concentration was maintained at 10% of the extender (v/v) in all the cases. Among the extenders, egg-yolk citrate and urea-egg-yolk mixed with 10% DMSO, methanol and ethanol produced 50% post-thaw spermatozoan motility, whereas DMA and glycerol provided only 10% motility. Trials on milt dilution ratio and cryoprotectant concentration are being conducted. Fertilization trials are also underway.

Breeding biology and monosex male seed production of Genetically Improved Farmed Tilapia (GIFT) strain of Oreochromis niloticus L. in Bangladesh

A study on the breeding biology of the GIFT strain of Nile tilapia, Oreochromis niloticus, was conducted for a period of five months. The sex ratio of the parent individuals was optimized for performance in spawn production, where the best results were obtained with a sex ratio of female to male of 4:1 compared to those of 3:1 and 2:1. The diameter of eggs obtained from the GIFT stock had major and minor axes of 2.19±0.09 and 1.72±0.07 mm, respectively, with no significant differences between the treatments. The average number of eggs produced was 392±22 per female, with fertilization and hatching rates ranging between 94-96% and 85-88%, respectively. No significant variation was observed between the treatments. Breeding frequencies per female in the three treatment groups ranged between 10-40 days and the highest value was obtained at a female to male sex ratio of 4: l. In an other experiment, l7 aMethyltestosterone (MT) was applied orally to the fry at their first feeding stage with treatments- 1, 2, 3 and 4 at the dosage of 100, 80, 60 and 40 mg/kg feed respectively, for the period of 28 days. The mean percentage of males obtained in treatments, 1, 2, 3 and 4 were 98, 97, 95 and 68, respectively. Treatments-1, 2 and 3 did not differ significantly (P>0.05) from each other but treatment 4 showed significant variation (P<0.05) from other treatments. The results showed that MT-100, 80 and 60 mg/kg feed administered for 28 days produced close to cent percent male population of the GIFT strains in aquaria.

Status of fish broodstock management and seed production in Bangladesh

The study was conducted with the broad objectives to assess the existing situation of broodstock management and fish seed production in private fish seed farms in Bangladesh. The data were collected from 100 private hatcheries and 40 nurseries in seven upazilas under four districts. There was no shed in forty hatcheries and the owners faced many problems. Brood fish ponds were found suitable for rearing brood fish. About 66% of the hatchery owners collected brood fish from their own ponds and ponds of neighboring areas. Activities like pond preparation; manuring and supplementary feeding were done properly but stocking density of brood fish in 76% of the hatcheries was 3,000-7,000 kg/ha. Infection of argulosis was found in brood fish of 87% of the hatcheries. About 67% of the hatchery owners practiced inter-species crossing. Major problems faced by the hatchery owners were argulosis of brood fish, unavailability of pure brood stock, inadequate brood fish pond. The hatchery owners were found interested to find out the preventive measures of argulosis, develop pure brood stock of indigenous carp and import pure strain of exotic carp. According to the nursery operators, they cultivated hybrid fry because of high demand, rapid growth and good taste. Problems of using hatchery spawn as mentioned by the nursery operators were inbreeding, under sized and aged brood stock, stunted growth, physical deformities and high mortality of spawn due to unknown causes.

Investment in fish seed multiplication farms in Bangladesh: Evidences of an attractive business

This study mainly evaluated the profitability of Fish Seed Multiplication Farms (FSMFs) having hatchery, nursery and hatchery-cum-nursery located in the districts of Jessore, Jhenidah and Narail in Bangladesh. The general findings of the study were that the investment in FSMFs with hatchery, nursery and hatchery-cum-nursery was highly profitable business. The results clearly indicated that the investment on hatchery was the most profitable than those of nursery and hatchery-cum-nursery operations from the viewpoints of individual investors. The results of sensitivity analysis suggested that the investment in nursery farm was a risky business with 20 per cent increase in operation and management as well was production costs or 20 per cent reduction in benefits if other things remaining the same. It was also evident from the study that the investors of FSMFs had currently been facing some crucial problems, which among others are: problems of inbreeding, shortage of brood fish, incidence of diseases, unavailability of certain inputs and lack of credit.

The effects of corona treatment on impact and spreading of ink-jet drops on a polymeric film substrate

The effects of varying corona surface treatment on ink drop impact and spreading on a polymer substrate have been investigated. The surface energy of substrates treated with different levels of corona was determined from static contact angle measurement by the Owens and Wendt method. A drop-on-demand print-head was used to eject 38 μm diameter drops of UV-curable graphics ink travelling at 2.7 m/s on to a flat polymer substrate. The kinematic impact phase was imaged with a high speed camera at 500k frames per second, while the spreading phase was imaged at 20k frames per secoiui. The resultant images were analyzed to track the changes in the drop diameter during the different phases of drop spreading. Further experiments were carried out with white-light intetferometry to accurately measure the final diameter of drops which had been printed on different corona treated substrates and UV cured. The results are correlated to characterize the effects of corona treatment on drop impact behavior and final print quality.

Seed production and culture techniques of Genetically Improved Farmed Tilapia (GIFT) in brackishwater environment

Effects of different levels of salinity on survival, growth and gonadal development of Genetically Improved Farmed Tilapia (GIFT) were studied under laboratory conditions in glass aquarium, for a period of ten weeks. The initial individual size of the GIFT was 20.23±4.45 and the salinity levels tested were 0, 5, 10, 15 and 20 ppt. The highest survival of 87.5% was found in 0 ppt and the lowest 60.5% in 20 ppt. Though the survival decreased progressively with increased salinity, there were no significant differences (P>0.05) among 0, 5, and 10 ppt. Similar to what has been observed in survival, the specific growth rate (SGR %/day) also decreased as of 1.30, 1.24, 1.08, 0.90 and 0.71, respectively, with the increased salinity of 0, 5, 10, 15 and 20 ppt. The gonadal development was highest in 0 ppt with a GSI value of 3.75 and lowest of 2.01 in 20 ppt. In the second experiment, gonadal development and seed production performance of GIFT in brackishwater condition were investigated for a period of three months. Each of the three fine meshed hapas of 20 square meters made from nylon net was placed in a freshwater (0 ppt) and in a brackish water (10-15 ppt) pond of the Brackishwater Station (BS). GIFT of 65 g average weight from a single cohort were stocked into three hapas at a rate of 2 per m. The male vs female ratio was 1:3. The development of gonad was faster with the higher gonadosomatic index (GSI %) of 3.85 % in freshwater condition than that of 2.73 % in brackish water. Within three months of the study period, a total of 70,510 and 44,250 GIFT fry were produced respectively, in freshwater and brackishwater conditions. Finally under third experiment, a participatory on-farm trial was carried out to evaluate the production performance of GIFT in monoculture and in polyculture with silver barb in coastal freshwater pond conditions. Nine ponds were selected for three treatment combinations of GIFT monoculture (T1), GIFT and silver barb polyculture (T2), and silver barb monoculture (T3). The ponds have been stocked in April, 05 at a density of 25,000 fry per ha. Fishes were fed with rice bran at the rate of 6% bw per day. In one month culture period, GIFT attained an average weight of 16.27 g in monoculture and 17.23 g in polyculture, against an average stocking weight of 0.37 g. Silver barb reached an average weight of 16.62 g in polyculture with GIFT and 10.01 g in monoculture, against an average stocking weight of 3.79 g.

Fish seed production and marketing in some selected areas of Bangladesh

The main objective of the study was to assess the production and marketing system of fish seed and examining the demand for and supply of fry and fingerlings in some selected areas viz. Mymensingh, Netrokona and Tangail in Bangladesh. The present study selected the related stakeholders like 20 Fish Seed Multiplication Farms (FSMF) owners, 80 nursery operators, 20 fry traders and 90 fish farmers who are the beneficiaries and directly involved with the fish seed production and supply chain, and marketing system of fish seed. In case of nursing and rearing fingerlings, only those stakeholders (nurserers and fish farmers) attached with NGOs were selected and the NGOs were GRAMUS, SARA and ORD from Mymensingh and SATU from Tangail district. Data were collected for the period 2004-2005 and most analyses were done by using tables and flow chart to determine production and marketing system of fish seed.