Preparation and properties of nido-carborane-specific monoclonal antibodies for potential use in boron neutron capture therapy for cancer.

As the first step of a research program aimed at developing a bispecific monoclonal antibody system for the delivery of boron-rich molecules to tumor cells for boron neutron capture therapy, monoclonal antibodies (mAbs) were produced against an anionic nido-carborane derivative, 4-[7,8-dicarbadodecahydroundecaborat(-1)-7-yl]butanoic acid. Two IgG subclass mAbs, designated HAW101 and HAW102, were identified that specifically bound the anionic nido-carborane hapten, as well as a variety of other anionic nido-carborane cage derivatives. By using surface plasmon resonance technology, the affinity constants of HAW101 and HAW102 were determined to be 1.9 x 10(9) and 6.8 x 10(8) M-1, respectively. A diverse array of 7-substituted and 7,8-disubstituted anionic nido-carborane derivatives reacted with the mAb HAW101 in competition ELISA, whereas anionic closo-polyhedral boranes showed negligible binding, suggesting a role for the open nido-carborane cage structure. These results suggest that mAbs such as HAW101, which bind anionic nido-carboranes, are useful in the development of bispecific mAbs for specific targeting and enhanced boron delivery to tumor sites.

Standard hand-book of household economy for the people : comprising plain directions for the management of a family, servants, lying-in room, nursery, sick-room, flower-garden, and household pets, and also, for the preparation and administration of remedies for disease /

Includes index.

The gardener's manual : containing plain instructions for the selection, preparation, and management of a kitchen garden : with practical directions for the cultivation and management of some of the most useful culinary vegetables /

Attributed to Charles F. Crosman. Cf. Richmond, M.L.H. Shaker literature.

Ochers and mineral pigments of the Pacific northwest occurrence, possible methods of preparation, and testing of ochers, siennas, and colored clays,

"This report represents a cooperative study between the Bureau of mines, Department of commerce, and the College of mines, University of Washington."

Investigations of the preparation and use of lignite, 1918-1925,

Running title: Lignite investigations, 1918 to 1925.

Preparation and characterization of analytical reference minerals /

Mode of access: Internet.

Preparation and serving of food in the garrison dining facility.

Includes index.

Bibliography of published literature on the preparation and properties of radioactive neutron sources.

"March 3, 1950."

Preparation and characterization of slush hydrogen and nitrogen gels.

Includes bibliographical references (p. 38-39).

Instruction manual for preparation and submission of revised estimate of cost of completing the interstate system in accordance with Section 104(b)5, Title 23, U.S. Code, Highways /

Cover title.

A study on the preparation and properties of phthalic acid,

Thesis (M.S.)--Univ. of California. May, 1916.

A practical treatise on the law and procedure involved in the preparation and trial of cases of ejectment and other actions at law respecting titles to land, treating particularly of the pleading, practice and evidence, and, in general way, also of the principles of substantive law involved in such actions,

Mode of access: Internet.

How to attract and hold an audience; a practical treatise on the nature, preparation, and delivery of public discourse,

Mode of access: Internet.

The pattern maker's assistant; embracing lathe work, branch work, ore work, sweep work, and practical gear construction; the preparation and use of tools; together with a large collection of useful and valuable tables.

Mode of access: Internet.