941 resultados para Lea


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O objetivo geral de este trabalho, a partir da perspectiva dos estudos da infncia, e focalizando numa aproximao de cunho documental, identificar os lugares da infncia numa experincia de comunicao comunitria, estabelecendo algumas categorias de analise que possam ser de utilidade para dialogar dita presena dentro do discurso do jornal comunitrio O cidado da Mar, um jornal comunitrio direcionado para as 16 favelas que compem a Mar, bairro situado na periferia da Zona Leopoldina do Rio de Janeiro. Assim, a autora coloca algumas provocaes para dialogar sobre os lugares estabelecidos para a infncia dentro das produes na comunicao alternativa, tal discusso faz parte de um dos mltiplos olhares sobre as representaes e lugares que so dados aos sujeitos sociais na comunicao comunitria, uma problematizao que precisa ser feita dentro do marco da democratizao da comunicao. As reflexes sobre a construo de uma metodologia de pesquisa de cunho documental conta com a contribuio de autores como Carlo Ginzburg e Marilia Amorim. As questes cidadania, comunicao comunitria, seus conceitos e recursos, a Mar e O cidado do bairro Mar, so colocadas a partir do dialogo principalmente com o equipe do jornal O cidado da Mar e as ideias dos autores: Antonio Gramsci, Jesus Martn-Barbero, Boaventura de Sousa, Raquel Paiva,Vito Gianotti, Adair Rocha e Andre Esteves. As reflexes sobre infncia, a potencia da narrao, enunciao e sujeito social so feitas a partir do que emergiu no campo de pesquisa, e dialogam com elementos e idias colocadas por Walter Benjamin, Bernard Charlot, Mikhail Bakhtin, Solange Jobim, Rita Ribes e Lucia Rabello.

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Rhipidodontini (Rhipidodonta Mrch, 1853 + Diplodon Spix in Wagner, 1827) grupo de bivalves de gua doce tem taxonomia complicada, devido s descries originais sucintas e muitas vezes pouco ou no ilustradas, somado a isto, estes bivalves carecem de uma reviso detalhada. Estas lacunas de informao tm gerando uma grande flutuao nas espcies consideradas vlidas pelos diferentes autores, dificultando a identificao das mesmas, assim como da biologia e distribuio. Assim, se props neste estudo a reviso taxonmica das espcies de Rhipidodontini nas bacias do alto rio Paran, rio So Francisco e rios costeiros do Atlntico Leste, Norte e Nordeste. Para alcanar este objetivo vistoriamos material em colees no Brasil e exterior. Coletas foram realizadas em diversas localidades para obteno de exemplares para descrio das partes moles e gloqudios. As informaes obtidas, somado ao descrito na literatura, foram utilizadas para traar um panorama de distribuio e conservao das espcies. As principais caractersticas das conchas foram utilizadas para elaborao de uma chave dicotmica para auxlio na identificao. Uma anlise morfomtrica foi empregada com o intuito de distinguir as espcies atravs da forma da concha. Reconhecemos Diplodon e Rhipidodonta includos na tribo Rhipidodontini. Em Diplodon foram identificadas seis espcies nas bacias estudadas: Diplodon ellipticus Spix in Wagner, 1827; Diplodon fontainianus (dOrbigny, 1835); Diplodon jacksoni Marshall, 1928; Diplodon multistriatus (Lea, 1831); Diplodon paulista (Ihering, 1893) e Diplodon rhombeus Spix in Wagner, 1827. Apesar de Diplodon granosus (Bruguire, 1792) possuir extensos registros na regio estuada, a espcie foi limitada a regio amaznica na nossa avaliao. Em Rhipidodonta, foi reconhecida uma nica espcie, Rhipidodonta garbei (Ihering, 1910). Entre estas espcies, temos algumas tradicionalmente reconhecidas como vlidas (e.g. D. ellipticus e D. granosus), contudo, outras foram revalidadas (e.g. D. jacksoni e R. garbei) e redefinidas perante a anlise do material tipo, partes moles e gloqudio. No foi possvel a eleio de uma nica caracterstica morfolgica para a separao das espcies, porm detalhes das brnquias, estmago, contorno da concha e escultura umbonal figuraram entre as mais utilizadas. Para a separao dos gneros de Rhipidodontini foram empregados atributos dos gloqudios (e.g. gancho gloquidial, protuberncia e forma do gloqudio) e das brnquias (e.g. forma da brnquia e conexo entre as lamelas). A chave dicotmica com base em caractersticas das conchas auxiliou a separar as espcies de Rhipidodontini. A anlise morfomtrica constituiu uma ferramenta til na separao das espcies, corroborando as identificaes prvias. Salientamos que o estudo aqui apresentado deve ser expandido para outras bacias hidrogrficas sul-americanas com o intuito de se conhecer a real diversidade destes bivalves de gua doce

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Adolescentes apresentam rpido crescimento e intensas mudanas corporais que os tornam vulnerveis em termos nutricionais. A prtica de restries alimentares, bastante comum entre adolescentes, pode levar a inadequaes nutricionais que parecem ser o primeiro sinal para o incio de uma desordem alimentar (DA). A participao feminina no esporte e o nmero de casos de DA em adolescentes atletas de modalidades que exigem exposio do corpo, agilidade e leveza dos movimentos, como o tnis, tm aumentado nos ltimos anos. As DA podem levar a complicaes de sade como irregularidades menstruais (IM) e baixa densidade mineral ssea (DMO), caracterizando a Trade da Mulher Atleta (TMA). Desta forma, acredita-se que alguns componentes dietticos podem ter associao com DA e seus agravos. O objetivo do presente estudo foi avaliar a associao de componentes dietticos com desordens alimentares, irregularidades menstruais e composio corporal em adolescentes atletas tenistas e no atletas do sexo feminino. Trata-se de estudo do tipo transversal. Foram realizadas avaliaes do desenvolvimento puberal pela auto-aplicao dos critrios de Tanner; da composio corporal pela absortometria radiolgica de dupla energia (DXA); dos parmetros dietticos por registro alimentar de trs dias alternados; das DA pela aplicao de trs questionrios validados (Eating Attitudes Test - EAT-26, Bulimic Investigatory Test, Edinburgh- BITE e o Body Shape Questionnaire - BSQ); do ciclo menstrual por questionrio validado e da DMO tambm pelo DXA. A Trade da Mulher Atleta (TMA) foi estabelecida pela presena concomitante de DA e/ou baixa disponibilidade de energia (BDE), IM e baixa DMO. Foram realizadas associaes por meio de correlaes de Spearman entre as variveis numricas de componentes dietticos com DA e composio corporal. Tambm foram realizadas associaes por meio do teste qui-quadrado, teste exato de Fisher ou prova binomial para as variveis categricas de adequao dos componentes dietticos com DA e seus agravos. Participaram do estudo 75 adolescentes (25 tenistas, 50 no atletas) apresentando desenvolvimento puberal similar. Atletas obtiveram melhor perfil da composio corporal quanto ao tecido adiposo. Quanto ingesto de macronutrientes, os carboidratos merecem destaque. Em ambos os grupos, a maioria das participantes apresentaram baixa ingesto de carboidratos, sendo este percentual de inadequao significativamente maior para as atletas. Os micronutrientes que obtiveram maior percentual de inadequao foram folato e clcio em ambos os grupos. Verificou-se que 92%, 32% e 24% das atletas e 72%, 8% e 30% das no atletas preencheram critrios para DA e/ou BDE, IM e baixa massa ssea, respectivamente. Apesar de adolescentes atletas tenistas e no atletas apresentarem prevalncia de DA similares, as no atletas apresentaram maior insatisfao com a imagem corporal pelo teste BSQ. No entanto, as atletas parecem estar em situao mais grave uma vez que apresentaram maior prevalncia de BDE e de IM. A DMO e a prevalncia de TMA foram similares entre os grupos. Foi verificada associao inversa e significativa entre alguns componentes dietticos (principalmente energia e carboidratos) e os escores do teste BSQ. Foi possvel concluir que a baixa ingesto de alguns componentes dietticos, principalmente energia e carboidratos, podem funcionar como marcadores para desordens alimentares em ambos os grupos a fim de previnir posteriores consequncias sade

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A tese objetiva construir, sob o ponto de vista dogmtico, critrios para a caracterizao do contrato incompleto no direito brasileiro, estabelecendo o trao distintivo de sua causa e a disciplina jurdica que lhe aplicvel, luz da metodologia civil-constitucional. Pretende-se, ainda, na perspectiva funcional dos fatos jurdicos, que permite a qualificao do contrato incompleto como negcio jurdico que emprega a tcnica da gesto negativa da lea normal dos contratos, definir parmetros interpretativos que orientem a sua execuo. Por representarem o esmorecimento voluntrio da tcnica regulamentar, os contratos incompletos exigem elevados padres de cooperao entre os contratantes na integrao das lacunas, a ensejar a incidncia reforada dos princpios da boa-f objetiva, da funo social e do equilbrio contratual, relativamente aos contratos em que ocorre a gesto positiva da lea normal. Aps investigar os mecanismos legais incidentes na hiptese de inadimplemento do dever de integrao da lacuna, analisam-se os limites legais e valorativos a que se sujeitam os contratos incompletos. Em chave conclusiva, almeja-se estabelecer o contrato incompleto como negcio jurdico lcito e merecedor de tutela no atendimento aos interesses concretos dos particulares no exerccio de suas atividades econmicas, a evidenciar os novos confins da autonomia privada na legalidade constitucional.

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, Boea hygrometrica (Bunge) R Br.cDNA4562cDNA, 434cDNA cDNANorthern blot434cDNA4236LEA42363LEA BhGRP1BhGLP1/ Northern blotRT-PCRBhGRP1BhGLP1PSORTN-BhGRP1-GFPBhGLP1-GFPBhGRP1-GFPBhGLP1-GFPBhGRP1GLPBhGRP1BhGLP1

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(Glycine max (L.) Meer.)34245% (Z22)cDNA-AFLP 4RT-PCR424 RACE2NCBI1SCHI (SCHISoybean chilling-induced gene)SCHI390bp14.2KDL34SOL34 (Soybean L34) RT-PCRSCHI18242418ABA (100M)PEG (30%10000)NaCl (250mM)SCHIABAPEGNaClSCHISCHISOL3424SOL34 SCHI26-29KD22SCHI-20 SCHISOL34L34 (42224h)2515 (10%) (20%LEAPM26) (10%) (20%)

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Unioni colidae1,U.(Anodontinatax)penicilla tussp.nov.,,Unionicola(Anodontinatax)penicillatus sp.nov.(111) ,1,,1998 11 11,A.woodianawoodiana(Lea)U.(Anodontinatax) inte

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Cyanobacteria perform photosynthesis and respiration in the thylakoid membrane, suggesting that the two processes are interlinked. However, the role of the respiratory electron transfer chain under natural environmental conditions has not been established. Through targeted gene disruption, mutants of Synechocystis sp. PCC 6803 were generated that lacked combinations of the three terminal oxidases: the thylakoid membrane-localized cytochrome c oxidase (COX) and quinol oxidase (Cyd) and the cytoplasmic membrane-localized alternative respiratory terminal oxidase. All strains demonstrated similar growth under continuous moderate or high light or 12-h moderate-light/dark square-wave cycles. However, under 12-h high-light/dark square-wave cycles, the COX/Cyd mutant displayed impaired growth and was completely photobleached after approximately 2 d. In contrast, use of sinusoidal light/dark cycles to simulate natural diurnal conditions resulted in little photobleaching, although growth was slower. Under high-light/dark square-wave cycles, the COX/Cyd mutant suffered a significant loss of photosynthetic efficiency during dark periods, a greater level of oxidative stress, and reduced glycogen degradation compared with the wild type. The mutant was susceptible to photoinhibition under pulsing but not constant light. These findings confirm a role for thylakoid-localized terminal oxidases in efficient dark respiration, reduction of oxidative stress, and accommodation of sudden light changes, demonstrating the strong selective pressure to maintain linked photosynthetic and respiratory electron chains within the thylakoid membrane. To our knowledge, this study is the first to report a phenotypic difference in growth between terminal oxidase mutants and wild-type cells and highlights the need to examine mutant phenotypes under a range of conditions.

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Reducing excessive light harvesting in photosynthetic organisms may increase biomass yields by limiting photoinhibition and increasing light penetration in dense cultures. The cyanobacterium Synechocystis sp. PCC 6803 harvests light via the phycobilisome, which consists of an allophycocyanin core and six radiating rods, each with three phycocyanin (PC) discs. Via targeted gene disruption and alterations to the promoter region, three mutants with two (pcpcTC) and one (CpcC1C2:pcpcTC) PC discs per rod or lacking PC (olive) were generated. Photoinhibition and chlorophyll levels decreased upon phycobilisome reduction, although greater penetration of white light was observed only in the PC-deficient mutant. In all strains cultured at high cell densities, most light was absorbed by the first 2 cm of the culture. Photosynthesis and respiration rates were also reduced in the CpcC1C2:pcpcTC and olive mutants. Cell size was smaller in the pcpcTC and olive strains. Growth and biomass accumulation were similar between the wild-type and pcpcTC under a variety of conditions. Growth and biomass accumulation of the olive mutant were poorer in carbon-saturated cultures but improved in carbon-limited cultures at higher light intensities, as they did in the CpcC1C2:pcpcTC mutant. This study shows that one PC disc per rod is sufficient for maximal light harvesting and biomass accumulation, except under conditions of high light and carbon limitation, and two or more are sufficient for maximal oxygen evolution. To our knowledge, this study is the first to measure light penetration in bulk cultures of cyanobacteria and offers important insights into photobioreactor design.

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Bioaccumulation of PCDD/F in the foodweb was investigated in the Ya-Er Lake area, which was heavily polluted by PCDD/F. The high concentrations of PCDD/F in sediment can be transferred and bioaccumulated by aquatic organisms and humans through various pathways. Benthonic invertebrate animals and aquatic plants with a lot of fibers in the root can accumulate PCDD/F from sediment and water. Snail (Bellamya aeruginosa), shrimp (Macrobranchium sp.) and freshwater mussel (Acuticosta chinensis (Lea)) took up PCDD/F from the water and maintained the emission patterns, whereas fish tended to selectively accumulate 2,3,7,8-substituted isomers. The tissues of fish-eating bird and duck (Anas platyrhynchos) were very highly contaminated by PCDD/F due to ingestion of fish and other aquatic organisms from sediment. The residual concentration in breast milk depended on the original concentration of PCDD/F in the food. A resident in Ya-Er Lake area showed a daily intake of PCDD/ F of about 9.14 pg TEQ/kg body weight/day. This is higher than the tolerable daily intake (TDI) for PCDD/F (1 pg TEQ/kg body weight/day), which was recommended by the World Health Organization (WHO). (C) 2001 Elsevier Science Ltd. All rights reserved.

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50% Hordeum vulgare ssp. vulgare84(water loss rate, WLR)12relative water content, RWCMDAfree-prolineLEA2(Dhn6)LEA3(HVA1)LEAdynamics water loss rate, DWLR2 h4 h8 h12 hSYBR GreenRT-PCRDhn6Dhn11Dhn13HVA1LEA 1. 84WLR0.086~0.205gh-1g-1DWWLR0.1gh-1g-1DWWLR0.18gh-1g-1DW61217.10~25.74 gg-1FW1232.99~53.45gg-1FW(P<0.05)(MDA)120.97~2.74nmolg-1FW121.46~4.74nmolg-1FW6WLRP<0.0510(TR1)14(TR2)8(TS1)QB24 (TS2) 2. (late embryogenesis abundant proteins, LEA proteins)LEA10148QB24RT-PCRDhn6HVA1Dhn6148Dhn61026bp510QB24963bp2222821DHN6DHN6HVA18QB241410661bp697bp694bp691bp1LEA3111014Gln32Arg33Ala195LEA311-LEA3 3. LEA10148QB242 h4 h6 h8 h10 h2~48242 h4 h8 h12 hRNASYBR GreenRT-PCR(Dhn6Dhn11Dhn13)LEA3(HVA1)Dhn6Dhn11Dhn13HVA1 Dhn6812Dhn11Dhn138122HVA11288~12LEADhn11LEALEADhn11Dhn12(dehydration responsive element, DRE) (1)(2) DHN6(3) 11-LEA3(4)LEADhn11(5)LEALEA Drought resistance was a complex trait which involved multiple physiological and biochemical mechanisms and regulation of numerous genes. Because its complex traits, it is difficult to understand the mechanisms of drought resistance in plants. Plants respond to water stress through multiple physiological mechanisms at the cellular, tissue, and whole-plant levels. Tibetan hulless barley, a pure line, is a selfing annual plant that has predominantly penetrated into the Qinghai-Tibetan Plateau and remains stable populations there. The wide ecological range of Tibetan hulless barley differs in water availability, temperature, soil type and vegetation, which makes it possess a high potential of adaptive diversity to abiotic stresses. This adaptive genetic diversity indicates that the potential of Tibetan hulless barley serves as a good source for drought resistance alleles for breeding purposes. 12 contrasting drought-tolerant genotypes were selected to measure relative water content (RWC), maldondialdehyde (MDA) and proline content, based on values of water loss rate (WLR) and repeated drought methods from Tibetan populations of cultivated hulless barley. As a result of the screening, sensitive and tolerant genotypes were identified to clarify relationships between characteristics of LEA2/LEA3 genes sequences and expression and drought-tolerant genotypes, associated with resistance to water deficit. In addition, dynamics water loss rate (DWLR) was measured to observe the changes on diffrential drought-tolerant genotypes. Real-time quantitative RT-PCR was applied to detect relative expression levels of Dhn6, Dhn11, Dhn13 and HVA1 genes in sensitive and tolerant genotypes with 2 h, 4 h, 8h and 12 h of dehydration. In the present study, differential sequences and expression of LEA2/LEA3 genes were explored in Tibetan hulless barley, associated with phenotypically diverse drought-tolerant genotypes. 1. The assessments of WLR and RWC were considered as an alternative measure of plant water statues reflecting the metabolic activity in plants, and the parameters of MDA and proline contents were usually consistent with the resistance to water stress. The values of detached leaf WLR of the tested genotypes were highly variable among 84 genotypes, ranging from 0.086 to 0.205 g/h.g DW. The 12 most contrasting genotypes (6 genotypes with the lowest values of WLR and 6 genotypes with the highest values of WLR) were further validated by measuring RWC, MDA and free-proline contents, which were well watered and dehydrated for 12 h. Results of RWC indicated that the values of 12 contrasting genotypes RWC ranged from 89.94% to 93.38% under condition of well water, without significant differences, but 6 genotypes with lower WLR had higher RWC suffered from 12 h dehydration. The results indicated that lower MDA contents, lower scores of WLR and higher proline contents were associated with drought-tolerant genotypes in hulless barley. Remarkably, proline amounts were increased more notable in 6 tolerant genotypes than 6 sensitive genotypes after excised leaves were dehydrated for 12 h, with control to slight changes under condition of well water. Results of MDA contents showed that six 6 tolerant genotypes had lower MDA contents than the 6 sensitive genotypes under both stressed and non-stressed conditions. As a result of that screening, drought- resistant genotypes (Ximala 10 and Pinbi 14) and drought-sensitive genotypes (Dongqing 8 and QB 24) were chosen for comparing the differential characteristics of LEA2/LEA3 genes and their expression analysis. It was conclusion that measurements of WLR could be considered an alternative index as screening of drought-tolerant genotypes in crops. 2. Late embryogenesis abundant (LEA) proteins were thought to protect against water stress in plants. To explore the relationships between configuration of LEA proteins and phenotypically diverse drought-tolerant genotypes, sequences of LEA genes and their deduced proteins were compared in Tibetan hulless barley. Results of comparing Dhn6 gene in Ximala 10 and QB24 indicated that absence of 63bp was found, except that only 5 mutant nucleotides were found. While 22 mutant sites were taken place in Dhn6 gene between sensitive and tolerant lines, 14 synonymous mutation sites appeared in the contrasting genotypes. The additional/absent polypeptide of 21 polar amino acid residues was not consistent with phenotypically drought-tolerant genotypes in hulless barley. It was deduced that synonymous mutation sites would play important roles in holding out right configurations and functions on DHN6 protein. The sequencing analysis results indicated that each cloned HVA1 gene from four selected genotypes contained an entire open reading frame. The whole sequence of HVA1 gene from Dongqing 8, QB24, Pinbi 14 and Ximala 10 was respectively 661bp, 697bp, 694bp and 691bp. Results of DNA sequence analyses showed that the differences in nucleotides of HVA1 gene in sensitive genotypes were not consistent with that of tolerant genotypes, except for absence of 33 nucleotides from +154 to +186 (numbering from ATG) in QB24. Database searches using deduced amino acid sequences showed a high homology in LEA3 proteins in the selected genotypes. Multiple sequence alignments revealed that LEA3 protein from Dongqing 8 was composed of 8 repeats of an 11 amino acid motif, less the fourth motif than Pinbi 14, Ximala 10 and QB24. Consistent mutant amino acid residues appeared in contrasting genotypes by aligning and comparing the coding sequence region, including Gln32, Arg33 and Ala195 in tolerant genotypes as compared to Asp32, Glu33 and Thr195 (Thr184 in Dongqing 8) in sensitive lines. It was concluded that consistent appearance of Gln32, Arg33 and Ala195 would contributed to functions of LEA3 protein in crops, as well as higher proportion of 11-amino-repeating motifs and polar amino acid residues. 3. Most of the LEA genes are up-regulated by dehydration, salinity, or low temperature, are also induced by application of exogenous ABA, which increases in concentration in plants under various stress conditions and acts as a mobile stress signal. Higher levels of proteins of LEA group 3 accumulated was correlated well with high level of desiccation tolerance in severely dehydrated plant seedlings. Dehydrins (DHNs), members of LEA2 protein, are an immunologically distinct protein family, and Dhn genes expression is associated with plant response to dehydration. Dynamic water loss rate was measured between sensitive genotypes and tolerant genotypes after they were dehydrated for 2 h, 4 h, 6h and 8 h. Detailed measurements of WLR at the early stage of dehydration (2, 4, 6, and 8 h) showed that WLR was stabilizing after 8 h, and there were no significant changes between these values and WLR after 24 h. Drought stress was applied to 10-day-old seedlings by draining the solution from the container for defined dehydration periods. Leaf tissues of the selected genotypes were harvested from control plants (time 0); and after 2, 4, 8, and 12 h of dehydration. Differential expression trends of Dhn6, Dhn11, Dhn13 and HVA1 genes were detected in phenotypically diverse drought-tolerant hulless barleys, related to different time of dehydration. Results of quantitative real-time PCR indicated that relative level of HVA1 expression was always higher in tolerant genotypes, rapidly increasing at the earlier stages (after 2-4 h of dehydration). However, HVA1 expressions of sensitive genotypes had a fast increase from 8 h to 12 h of stress. Significant differences in expression trends of dehydrin genes between tolerant genotypes and sensitive lines were detected, mainly in Dhn6 and Dhn13 gene, depending on the duration of the dehydration stress. The relative expression levels of Dhn6 gene were significantly higher in tolerant genotypes after 8 h dehydration, by control with notable higher expression levels after 12 h water stress in sensitive ones. The relative expression levels of Dhn13 gene tended to ascend during exposure to dehydration in drought-sensitive genotypes. However, fluctuate trends of Dhn13 expression level were detected in drought-resistant lines, including in lower expression levels of 12 h dehydration as compared to 8 h water stress. It was conclusion that (1) diverse LEA proteins would play variable roles in resisting water stress in plants; (2) expression of Dhn11 gene was not induced by dehydrated signals because of the trends of expression descended in contrasting genotypes suffered from water deficit and (3) variable accumulations on LEA proteins would be appear in diverse drought-tolerant genotypes during dehydrations. It is deduced that higher accumulations of Dhn6 and Dhn13 expression in 8 h dehydration are related to diverse drought-tolerant lines in crops. The present results indicated that different dehydrin genes would play variable functional roles in resisting water stress when plants were suffered from water deficit. The authors suggest physiologically different reactions between resistant and sensitive genotypes may be the results of differential expression of drought-resistant genes and related signal genes in plants. In addition, contrarily induced expression of Dhn11 and Dhn12 was related to dehydration responsive element (DRE) in barleys. The present study indicated that (1) measurements of WLR and RWC could be considered as one index of drought-tolerant screenings; (2) synonymous mutation sites would play important roles in holding out right configurations and functions on DHN6 protein, (3) higher proportion of 11-amino-repeating motifs and polar amino acid residues would contribute to functions on LEA3 protein, (4) the longer drought, the more accumulation on LEA proteins, except for Dhn11 gene in crops and (5) differential responses on expression of LEA protein genes would result in physiological traits of drought tolerance in plants.

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(Gossypium hirsutum L.Zhongmain No.2 3) ,- - , , , : , , ; , , , , , 75% 40 % , , 75% ,

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SSTMg/CaSSTSSTICP-OESMg/Ca 53(N1840.23E13537.113225m)Globigerinoides ruberMg/CaMg/CaMg(30%H2O20.1M NaOH90)(250l 0.001N HNO3 10S)(ICP-OES)Mg/CaAr0.5L/min0.5L/min-1.5L/min0.2Mpa0-0.4Mpa20rpm0-125rpmRF1150W750W-1500W15.5mm8-21mmMg/Ca=3.333mmol/molMg/Ca<0.5%,1%53Mg/CaRSD2.7%Lea(2000)G. ruber()Mg/CaSST:Mg/Ca(mmol/mol) 0.30exp[0.089SST()]SST280.3

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,WIA,