Improving aquaculture feed in Bangladesh: From feed ingredients to farmer profit to safe consumption

Use of manufactured feeds in aquaculture in Bangladesh has grown rapidly over the last five years. More than 1 million tonnes of commercially formulated feeds and 0.3-0.4 million tonnes of farm-made feeds were produced in 2012, and sectoral growth is projected to increase substantially over the medium term. This working paper summarizes findings from a study, conducted as part of the WorldFish/USAID “Feed the Future-Aquaculture” project in 2012, assessing the current status of the aquaculture feed sector in Bangladesh. Fish feed value chains, market trends, ingredients and formulation systems, farm feeding practices, ancillary services and feed regulations were investigated. The study identifies a number of entry points for interventions in the sector, and investments which would improve feed quality and farmer access to better feeds and support the growth of sustainable aquaculture.

Suggestive role of eye-stalk of Macrobrachium gangeticum Bate, 1868 in regulation of its reproductive behaviour

Role of eye-stalk of Macrobrachium gangeticum Bate, 1868 in its reproductive behaviour has been examined by conducting deletion and addition experiments. Eye-stalk ablation induced gonadal maturity in both sexes, leading to change in colour and size of ovaries and increase in GSI and oocyte diameter in females and increased length of testes and diameters of seminiferous tubules in males. Injection of eye-stalk extracts tended to at least partly restrict the effects in both sexes. The experiments thus suggested that the eye-stalk of M. gangeticum released some gonad inhibiting factors.

Embryonic stages and eye-specific gene expression of the local cyprinoid fish Anabarilius grahami in Fuxian Lake, China

Anabarilius grahami is a cyprinoid fish endemic to Fuxian Lake, Yunnan, China. In this study, a comprehensive staging series of A. grahami was produced. The embryonic development of A. grahami was divided into six main periods: zygote period, cleavage per

Improved handling and preservation of freshwater giant prawn, Macrobrachium rosenbergii for producing safe and wholesome products

Studies were undertaken to evaluate the quality changes in freshwater giant prawn, Macrobrachium rosenbergii during various storage conditions of handling and preservation and producing safe and quality products. The samples kept in ice immediately after catch with head-on and head-less condition were found to be acceptable for 6 days and 7 days, respectively. Delaying of icing considerably shortened the shelf-life. The pH value increased from 6.36 to 8.0 after 10 days in ice. The initial average TVB-N value of sample increased from below 10 mg/100 g to 25 mg/100 g with the lapse of storage period. The Ca++ ATPase activity in presence of 0.1M KCl slightly decreased at the end of 10 days of ice storage. Immediately after harvest, initial aerobic plate count (APC) was 2.88x10^6 CFU/g which gradually increased to 1.12x10^8 CFU/g after 6 days in ice storage and showed early signs of spoilage. Initial bacterial genera in the prawn iced at 0 hours were comprised of Coryneform (22.21 %), Bacillus (7.40%), Micrococcus (11.11 %), Achromobacter (48.14%), Flavobacterium/Cytophaga (7.40%), Pseudomonas (3.70%) and Aeromonas (3.70%). During ice storage Coryneforms and Bacillus were always dominating along with less prominent ones - Micrococcus, Achromobacter and Flavobacterium. Studies were conducted on the stability of myofibrillar protein of M. rosenbergii under different storage and pH conditions. The influence of a wide range of pH on the remaining Ca++ ATPase activity of M. rosenbergii muscle myofibrils after storage at -20°C for 2 days, at 0°C for 2 days and at 35°C for 30 minutes demonstrated that ATPase activities were lower in acidic and alkaline pH regions and the activity remained relatively high. Mg++ ATPase activities both in presence and absence of Ca++ remained high at neutral pH compared to those of acidic and alkaline region. The solubility of myofibrillar protein decreased gradually both in acidic and alkaline pH regions. The study also examined the bacteriological quality of freshly harvested M. rosenbergii, pond sediment and pond water from four commercial freshwater prawn farms at Fulpur and Tarakanda upazilas in the district of Mymensingh. The study included aerobic plate count (APC), total coliform count, detection, isolation and identification of suspected public health hazard bacteria and their seasonal variation, salt tolerance test, antibiotic sensitivity test of the isolates and washing effect of chlorinated water on the bacterial load in the prawn samples. APC in sediment soil and water of the farm and gill and hepatopancreas of freshly harvested prawns varied considerably among the farms and between summer and winter season. The range of coliform count in water, gill and hepatopancreas ranged between 6 - 2.8x10^2 CFU/ml, 1.2x10^2 - 3.32x10^2 CFU/g and 1.43x10^2 - 3.89 x10^3 CFU/g, respectively. No coliform was detected in pond sediment sample. Suspected health hazard bacteria isolated and identified from pond sediment, water, gill and hepatopancreas included Streptococcus, Bacillus, Escherichia coli, Klebsialla, Salmonella, Staphylococcus, Pseudomonas and Aeromonas. Bacillus, Salmonella and Staphyloccus [sic], and were found to be highly salt tolerant and capable of growing at 10% NaCl. The antibiotic discs with different concentration of antibiotics were used for the sensitivity test. The organisms were found to be most sensitive against Tetracyclin and Gentamycin.

Electrophoretic characteristics of oil sardine (Sardinella longiceps) and mackerel (Rastrelliger kanagurta) eye lens proteins

Electrophoresis of eye lens proteins of oil sardine and mackerel showed separation of proteins into three and four components, indicating the heterogeneous nature of the population.

On the eye diameter-total length relationship of the Indian mackerel Rastrelliger kanagurta (Cuvier)

Results of studies of chemical, bacteriological and organoleptic quality of cured fish collected from four major curing centres along the Tamil Nadu coast are reported. Only 32.43% of the samples had moisture level below 35%, 0.9% had salt 25% and above. None of the samples showed acid insoluble ash below 1.5%. The main defects were unhygienic processing, inadequate salting, use of poor quality salt and incomplete drying. Recommendations for improvement of quality are given.

Use of eye lens diameter and weight as an age indicator in the carangid fish, Decapterus russelli (Pisces: Carangidae) from Gulf of Oman: preliminary observation

Specimens of Decapterus russelli have been collected from Lema, north of the Gulf of Oman. The ocular lens diameter and weight were tested as an additional age indicator to those already in use. The results showed that this technique could be adopted for determining the age of the species Decapterus russelli when the specimens are in the second year of age in case of eye lens diameter. On the other hand, eye lens weight failed to separate between the four age groups observed. The method is especially useful for age determination when otolith or scale ring are not visible or when false rings give erroneous reading.

Age-related effects of bilateral frontal eye fields lesions on rapid eye movements during REM sleep in rhesus monkeys

Rapid eye movement (REM) is one of the most characteristic features of REM sleep, but the mechanisms underlying its regulation remain unclear. The present study aims to investigate whether the frontal eye field (FEF) is involved in the regulation of the r