964 resultados para . Trans-descendência. Liberdade
Resumo:
Este trabalho uma releitura dos filsofos anarquistas Mikhail Bakunine e Max Stirner de modo a apurar uma esttica a partir da sua ontologia anrquica. Outras fontes so usadas no sentido de mostrar a relao antagnica do anarquismo com a soberania e com a representatividade. O contedo abordado problematiza uma ideia de arte de viver que consiste na combinaao do nada criativo de Stirner com a concepo de Natureza produto-produtora de Bakunine. Enquanto o primeiro constitui o cerne ontolgico do indivduo, o ltimo est relacionado com a ideia de desejo elaborada por Deleuze e Guattari. A afirmao do desejo como produo do real torna o nada criativo de Stirner a morada ntima de criao do mundo
Resumo:
A finalidade da investigao inquirir o modo como o problema do nihilismo se acha concebido no confronto entre Heidegger e Jnger. Para esse efeito, toma-se como ponto de partida dois textos: por um lado, o ensaio de Jnger intitulado ber die Linie, publicado em 1949, na Festschrift dedicada a Heidegger por ocasio dos seus sessenta anos; por outro lado, a resposta dada por Heidegger, em 1955, no seu contributo para uma Festschrift anloga, dedicada a Jnger resposta originalmente publicada com o ttulo ber die Linie (e posteriormente republicada com o ttulo Zur Seinsfrage).
Resumo:
O dispositivo publicitrio sempre teve uma presena totalitria, no sentido de espao e tempo, na vida dos indivduos na sociedade de consumo (fala-se de 200 a 5 mil impactos dirios). Nos anos mais recentes, a introduo de ferramentas digitais e a instituio de novos circuitos de comunicao levou a indstria publicitria a uma mudana de paradigma. A grande transformao est a acontecer ao nvel das linhas de visibilidade, uma vez que a publicidade tradicional, baseada nos mass media, vem perdendo influncia para os circuitos one-to-one. A crescente migrao para os equipamentos pessoais e mveis (computadores, tablets ou smartphones) abriu caminho para novas formas de comunicao entre as marcas e os consumidores, com circuitos mais fechados ou mesmo personalizados. A alterao dos circuitos relacionais permite identificar um novo contexto: estamos a viver a era da publicidade invisvel. Hoje a mensagem pode ser enviada apenas a pblicos especficos, melhor identificados e por isso mais qualificados, passando longe do olhar dos consumidores que no interessa atingir. A introduo dessas ferramentas digitais institui uma nova economia do poder, uma vez que tornou a mensagem publicitria mais eficaz para fazer circular a mercadoria.
Resumo:
Ne bis in idem, understood as a procedural guarantee in the EU assumes different features in the AFSJ and in european competition law. Despite having a common origin (being, in both sectors the result of the case law of the same jurisdictional organ) its components are quite distintic in each area of the integration. In the AFSJ, the content of bis and idem are broader and addressed at a larger protection of individuals. Its axiological ground is based on the freedom of movements and human dignity, whereas in european competition law its closely linked to defence rights of legal persons and the concept of criminal punishment of anticompetitive sanctions as interpreted by the ECHRs jurisprudence. In european competition law, ne bis in idem is limited by the systemic framework of competition law and the need to ensure parallel application of both european and national laws. Nonetheless, the absence of a compulsory mechanism to allocate jurisdiction in the EU (both in the AFSJ and in the field of anti-trust law) demands a common axiological framework. In this context, ne bis in idem must be understood as a defence right based on equity and proportionality. As far as its international dimension is concerned, ne bis in idem also lacks an erga omnes effect and it is not considered to be a rule of ius cogens. Consequently, the model which the ECJ has built regarding the application of the ne bis in idem in transnational and supranational contexts should be replicated by other courts through cross fertilization, in order to internationalize that procedural guarantee and broaden its scope of application.
Resumo:
The trans-apical aortic valve implantation (TA-AVI) is an established technique for high-risk patients requiring aortic valve replacement. Traditionally, preoperative (computed tomography (CT) scan, coronary angiogram) and intra-operative imaging (fluoroscopy) for stent-valve positioning and implantation require contrast medium injections. To preserve the renal function in elderly patients suffering from chronic renal insufficiency, a fully echo-guided trans-catheter valve implantation seems to be a reasonable alternative. We report the first successful TA-AVI procedure performed solely under trans-oesophageal echocardiogram control, in the absence of contrast medium injections.
Resumo:
Beta-oxidation of the conjugated linoleic acid 9-cis,11-trans-octadecadienoic acid (rumenic acid) was analyzed in vivo in Saccharomyces cerevisiae by monitoring polyhydroxyalkanoate production in the peroxisome. Polyhydroxyalkanoate is synthesized by the polymerization of the beta-oxidation intermediates 3-hydroxyacyl-CoAs via a bacterial polyhydroxyalkanoate synthase targeted to the peroxisome. The amount of polyhydroxyalkanaote synthesized from the degradation of rumenic acid was found to be similar to the amount synthesized from the degradation of 10-trans,12-cis-octadecadienoic acid, oleic acid or 10-cis-heptadecenoic acid. Furthermore, the degradation of 10-cis-heptadecenoic acid was found to be unaffected by the presence of rumenic acid in the media. Efficient degradation of rumenic acid was found to be independent of the Delta(3,5),Delta(2,4)-dienoyl-CoA isomerase but instead relied on the presence of Delta(3),Delta(2)-enoyl-CoA isomerase activity. The presence of the unsaturated monomer 3-hydroxydodecenoic acid in polyhydroxyalkanoate derived from rumenic acid degradation was found to be dependent on the presence of a Delta(3),Delta(2)-enoyl-CoA isomerase activity. Together, these data indicate that rumenic acid is mainly degraded in vivo in S. cerevisiae through a pathway requiring only the participation of the auxiliary enzymes Delta(3),Delta(2)-enoyl-CoA isomerase, along with the enzyme of the core beta-oxidation cycle.
Resumo:
(A) Most azobenzene-based photoswitches require UV light for photoisomerization, which limit their applications in biological systems due to possible photodamage. Cyclic azobenzene derivatives, on the other hand, can undergo cis-trans isomerization when exposed to visible light. A shortened synthetic scheme was developed for the preparation of a building block containing cyclic azobenzene and D-threoninol (cAB-Thr). trans-Cyclic azobenzene was found to thermally isomerize back to the cis-form in a temperature-dependent manner. cAB-Thr was transformed into the corresponding phosphoramidite and subsequently incorporated into oligonucleotides by solid phase synthesis. Melting temperature measurement suggested that incorporation of cis-cAB into oligonucleotides destabilizes DNA duplexes, these findings corroborate with circular dichroism measurement. Finally, Fluorescent Energy Resonance Transfer experiments indicated that trans-cAB can be accommodated in DNA duplexes. (B) Inverse Electron Demand Diels-Alder reactions (IEDDA) between trans-olefins and tetrazines provide a powerful alternative to existing ligation chemistries due to its fast reaction rate, bioorthogonality and mutual orthogonality with other click reactions. In this project, an attempt was pursued to synthesize trans-cyclooctene building blocks for oligonucleotide labeling by reacting with BODIPY-tetrazine. Rel-(1R-4E-pR)-cyclooct-4-enol and rel-(1R,8S,9S,4E)-Bicyclo[6.1.0]non-4-ene-9-ylmethanol were synthesized and then transformed into the corresponding propargyl ether. Subsequent Sonogashira reactions between these propargylated compounds with DMT-protected 5-iododeoxyuridine failed to give the desired products. Finally a methodology was pursued for the synthesis of BODIPY-tetrazine conjugates that will be used in future IEDDA reactions with trans-cyclooctene modified oligonucleotides.
Resumo:
Tesis (Maestra en enfermera, con especialidad en Administracin de Servicios de Enfermera) U.A.N.L.
Resumo:
Tesis (Maestra en Ciencias en Nutricin) UANL, 2012.
Resumo:
Le dveloppement ainsi que lamlioration des diffrentes techniques de purification sont des dfis importants pour la chimie daujourdhui. Certaines des mthodes actuelles, tel que le greffage dun ractif sur un support solide permettant daccder un produit pur par simple filtration du milieu, comportent toutefois certains inconvnients. En effet, les proprits de solubilit de ces polymres rendent la mise en uvre des ractions plus difficiles. Cest dans ce contexte que le groupe du Pr. Charette a rapport lutilisation de ractifs lis un sel de ttraarylphosphonium (TAP). Ces sels peuvent tre solubiliss dans un solvant tel que le dichloromthane et aisment retirs du milieu ractionnel par prcipitation laide dther dithylique (Chapitre 1). Lun des objectifs de cette thse a donc t li la dcouverte de deux mthodes complmentaires qui, jusqu prsent, sont considres comme des mthodes de choix pour la synthse des sels de TAP fonctionnaliss (Chapitre 2). Lune dentre elles est utilise par Soluphase inc., une entreprise qui commercialise ces sels de TAP. Lefficacit des sels en tant que support dans la synthse de petites molcules a t dmontre lors de la synthse dun produit naturel, la ()-coniine (Chapitre 3). Lisolement des intermdiaires synthtiques instables par simple prcipitation laide dun support de TAP a permis de rendre cette synthse plus efficace que celle dj connue. Dans le deuxime volet de cette thse, plusieurs problmes relis la synthse de drivs cyclopropaniques 1,1-disubstitus ont t tudis. Ces derniers font partie intgrale de plusieurs produits naturels et de mdicaments. Cependant, leur formation par une raction de cyclopropanation dalcnes utilisant des ractifs diazoques possdant deux groupements de type accepteur nest pas tche facile (Chapitre 4). En effet, cette raction souffre dun faible contrle diastroslectif. Par le fait mme, trs peu de mthodologies de synthse ont rapport lutilisation de ce type de ractifs diazoques dans des ractions de cyclopropanation stroslectives. Ltude du mcanisme de la raction de cyclopropanation catalyse au Rh(II) a propos des indices favorisant un modle ayant des prcdents dans la littrature (Chapitre 5). Ces tudes nous ont men la dcouverte de la capacit trans-directrice du groupement amide lors des ractions de cyclopropanation dolfines. Nous avons donc utilis cette proprit afin de rsoudre plusieurs problmes rencontrs dans la littrature. Nous avons montr quelle permet laccs des drivs cyclopropaniques possdant deux groupements carboxyliques gminaux avec des slectivits leves (Chapitre 6). Ces produits taient accessibles que par des squences synthtiques ncessitant plusieurs tapes. De plus, nous avons dmontrs que ces nouveaux drivs cyclopropaniques sont des outils synthtiques fort utiles dans la synthse de produits naturels dintrt biologique. Cette formidable capacit trans-directrice du groupement amide nous a permi de rsoudre le problme de la synthse asymtrique de drivs carboxyliques -cyano cyclopropaniques (Chapitre 7). De plus, ce projet nous a menes la dcouverte de leffet de divers additif achiraux permettant daugmenter la slectivit dans certaines ractions. Cette raction possde une vaste tendue et lutilit de ces nouveaux drivs cyclopropaniques a t dmontre par plusieurs transformations de groupements fonctionnels.
Resumo:
Tesis (Doctorado en Ciencias con Acentuacin en Alimentos) UANL, 2013.
Resumo:
Diplonema papillatum est un organisme unicellulaire qui vit dans locan. Son gnome mitochondrial possde une caractristique spciale: tous les gnes sont briss en de multiples fragments qui sappellent modules. Chaque module est cod par un chromosome diffrent. Lexpression dun gne exige des pissages-en-trans qui assemblent un ARN messager complet partir de tous les modules du gne. Nous avons prcdemment montr que le gne cox1 est encod dans neuf modules avec six Us non encods entre le module 4 et le module 5 de lARN messager mature [1]. Nous navons identifi aucune squence consensus connue de site dpissage prs des modules. Nous spculons quun ARN guide (gRNA) a dirig lpissage-en-trans du gne cox1 par un mcanisme qui est semblable ldition dARN par linsertion/la suppression des Us chez les kintoplastides, le groupe sur des diplonmides. Nous avons trouv que les six Us sont ajouts au bout 3 de lARN dune faon semblable ceux ajouts par le TUTase lors de ldition de linsertion des Us chez les kintoplastides. Nous avons construit des profils de gRNA de lpissage-en-trans avec les expressions rgulires bas sur notre connaissance des gRNAs dans ldition dARN chez les kintoplastides. Selon la complmentarit partielle entre le gRNA et les deux modules adjacents, nous avons gnr des amorces pour RT-PCR visant dtecter des squences qui sont assorties un des profils de gRNA. Une exprience pilote in vitro na pas permis de reconstituer lpissage-en-trans des modules 3, 4, et 5, suggrant que nous devons amliorer nos techniques.
