彩绒革盖菌固体发酵生产木质素酶工艺优化研究

采用均匀设计U15( 5 8)和双温度培养法进行彩绒革盖菌固体发酵生产木质素酶 ,对漆酶、愈创木酚酶、多酚氧化酶的活力进行回归分析。结果表明 :应用双温度培养法进行彩绒革盖菌固体发酵生产木质素酶时 ,在自然补给氧气 ,培养基pH自然 (约 6 5 ) ,并保持环境湿度约 60 %的条件下 ,2 0d是适宜的发酵周期 ;玉米浆、麸皮、 (NH4 ) 2 SO4 、水分适宜作为固体培养基的成分 ;少量的Tween 80有利于木质素酶的生产。

Artificial neural network classification based on high-performance liquid chromatography of urinary and serum nucleosides for the clinical diagnosis of cancer

Nucleosides in human urine and serum have frequently been studied as a possible biomedical marker for cancer, acquired immune deficiency syndrome (AIDS) and the whole-body turnover of RNAs. Fifteen normal and modified nucleosides were determined in 69 urine and 42 serum samples using high-performance liquid chromatography (HPLC). Artificial neural networks have been used as a powerful pattern recognition tool to distinguish cancer patients from healthy persons. The recognition rate for the training set reached 100%. In the validating set, 95.8 and 92.9% of people were correctly classified into cancer patients and healthy persons when urine and serum were used as the sample for measuring the nucleosides. The results show that the artificial neural network technique is better than principal component analysis for the classification of healthy persons and cancer patients based on nucleoside data. (C) 2002 Elsevier Science B.V. All rights reserved.

科尔沁沙地乌兰敖都地区荒漠化动态监测

利用科尔沁沙地乌兰敖都荒漠化试验站的监测资料,从植被、土壤、水分、气象和沙尘暴等方面分析了乌兰敖都地区的荒漠化发展动态。结果表明:近10年内气温有暖温化趋势,但灾害天气没有明显增加;流动沙丘侵蚀最严重,沙丘平均移动速率为2.2 m/a;人工植被发展良好,但原生草场的可持续发展能力有所下降;地下水位没有明显下降趋势。目前,科尔沁沙地乌兰敖都地区土地荒漠化呈逆转趋势,整体环境有所改善。

黄土高原典型地区土壤侵蚀研究

本文对黄土高原典型地区土壤侵蚀以往的研究结论进行了综合分析 ,结果表明 :(1 )影响土壤侵蚀的主要因素为降雨、地形及土地利用 ;(2 )土壤侵蚀主要类型为水蚀及重力侵蚀 ;(3)土壤侵蚀主要集中在汛期 ;(4)土壤侵蚀具有垂直分带性 ;(5)在研究的四个典型地区中 :绥德地区侵蚀严重 ,天水地区侵蚀相对较轻微 ,安塞地区侵蚀典型 ,西峰地区侵蚀特殊

农业高效用水理论研究综述

农业高效用水包括节水灌溉和旱作农业 ,其核心是提高自然降水和灌溉水的利用效率和效益 .农田蒸散的测定方法各有利弊 .FAO先后建议用Penman修正式和Penman Monteith公式计算参考作物蒸散量 .Jensen乘法模型和Blank加法模型在作物水分生产函数研究中得到广泛应用 .土壤适宜含水量和土壤干旱下限指标的最新研究成果 ,为低定额的农业供水提供了土壤物理学的重要依据 .水分亏缺对与产量形成相关的各个生理过程影响的先后顺序为细胞扩张 >气孔运动 >蒸腾运动 >光合作用 >物质运输 .不很严重的干旱反而对物质运输有促进作用 .农田灌溉研究已由传统的充分灌溉 ,转向非充分灌溉、调亏灌溉和控制性分根交替灌溉 .未来农业高效用水理论将在界面、土壤水动力学、生物节水、缺水逆境等方面深入开展研究 .

森林生态系统硅素循环研究进展

硅是植物生长发育的有益元素,其在生态系统内的迁移转化是维持生态系统结构与功能的决定性因素之一。近年来,陆地生态系统硅循环特别森林生态系统硅循环在全球生物地球化学循环中的重要性,受到越来越多的关注。该文总结了国内外森林生态系统硅循环研究的成果,在综述了硅在森林生态系统中的存在形态、分布、循环过程的基础上,总结了森林生态系统硅循环的特点、作用及其影响因素,并指出典型森林生态系统类型中硅循环规律的研究、森林生态系统与其它生态系统硅循环的比较研究、森林生态系统硅循环对全球气候变化的影响和响应研究和人类干扰对森林生态系统硅循环的影响的研究将是今后开展森林生态系统硅循环研究的重点。

沿海防护林体系生态环境效益及评价技术

本文是“八五”国家科技攻关专题“沿海防护林体系生态经济效益及其评价技术”的阶段性研究成果，在国内首次系统地阐明了沿海防护林体系的中尺度气象效应、区域性气候效应、降盐改土效应、海堤林带的固土护堤效应和沿海农田林网的抗灾保产效应。

远紫外辐射对紫杉幼苗针叶膜脂过氧化及内源保护系统的影响

实验室条件下用远紫外线 (UV BC)光源照射紫杉幼苗 ,随照射时间延长 ,针叶的离子渗出率、膜脂过氧化水平、组织自动氧化速率及H2 O2 含量显著增加 ,可溶性蛋白、抗坏血酸、类胡萝卜素和叶绿素含量下降 ,叶绿体光系统II电子传递活性显著下降 ,外源活性氧清除剂苯甲酸钠和抗坏血酸对针叶膜脂过氧化有抑制作用 ;甲基紫精和DDC对针叶膜脂过氧化有促进效果 ,远紫外线引起的紫杉伤害可能和针叶树的越冬光氧化伤害有类似之处 .紫杉苗对紫外辐射的抗性远高于一般农作物 .

中国东北西部地区沙质荒漠化过程与植被动态关系的生态学研究:植被的分类

根据东北沙质荒漠化地区呼伦贝尔沙地、科尔沁沙地的 72个沙地植物群落样地、2 76种植物的调查数据 ,进行双向指示种分析 (TWINSPAN) ,结合DCA排序得到符合客观实际的分类结果 ,初步建立了中国东北沙质荒漠化地区植被分类系统 ,6个植被型 ,11个植被亚型 ,40个群系 ,5 5个群丛

资源区域流动的驱动因子分析与流动潜力测度:以晋煤输出为例

论文采用国家铁路省际间煤炭资源的交流数据、动力煤价格数据,以全国最大的煤炭输出省--山西为例,运用回归分析等统计方法,分析了资源区域流动的驱动因子。基于场论,提出了测度资源区域流动潜力的方法。主要结论有:①资源区域流动的主要驱动因子有距离、价格差;②在显著性水平α=0.05下,区域煤炭价格差与晋煤净输流量呈正向变化,但线性无关,源汇间距离与晋煤净输流量呈线性负相关;③2005年晋煤净输流量与源汇间距离符合F=e9.091-0.003D的Growth回归模型;④资源势差涵盖了影响煤炭流动的流量和距离等2个主

内蒙古鄂温克族牧民砖茶型氟铝联合中毒调查

目的调查鄂温克族牧民砖茶型氟铝联合中毒病情。方法随机对鄂温克族16~55岁男女均衡62名牧民进行发、血清、尿氟铝水平及血清电解质、生物化学检查,同时检测饮水、牛奶、砖茶、奶茶等氟铝水平,并进行临床检诊和拍摄前臂、骨盆正位X线片,不饮用砖茶的16~55岁男女均衡41名居民为对照组。结果发、血清、尿铝水平牧民组分别为(24.60±9.82)mg/Kg、(0.78±0.25)、(2.84±0.65)mg/L,对照组分别为(12.50±4.55)mg/kg、(0.18±0.11)、(2.09±0.64)mg/L;发、血清、尿氟水平牧民组分别为(1.00±0.52)mg/kg、(0.10±0.10)、(2.74±1.42)mg/L,对照组分别为(1.39±1.34)mg/kg、(0.04±0.03)、(0.87±0.43)mg/L;牧民发、血、尿铝及血、尿氟显著高于对照组(P<0.01)。牧民组血清AST、LDH、HBDH、ALP显著高于对照组(P<0.01)。X线摄片牧民组氟骨症检出率50.00%,关节退行性变检出率61.29%,骨间膜骨化33.87%,疏松型改变检出率29.03%,硬化型改变检出率8.06%;对照组氟骨症检出率2.44%,退行性变检出率34.15%,疏松型改变检出率21.95%,骨间膜骨化检出率2.44%,硬化型改变2.44%,X线氟骨症、关节退行性变、骨间膜骨化牧民组均高于对照组(P<0.01)。结论内蒙古鄂温克族牧民饮茶型氟中毒病情较为严重,其发病机理较为复杂,并导致多组织、器官损伤,可能为砖茶型氟铝联合中毒。

Co-electrospun poly(lactide-co-glycolide), gelatin, and elastin blends for tissue engineering scaffolds

In this study, we describe composite scaffolds composed of synthetic and natural materials with physicochemical properties suitable for tissue engineering applications. Fibrous scaffolds were co-electrospun from a blend of a synthetic biodegradable polymer (poly(lactic-co-glycolic acid), PLGA, 10% solution) and two natural proteins, gelatin (denatured collagen, 8% solution) and (x-elastin (20% solution) at ratios of 3:1:2 and 2:2:2 (v/v/v). The resulting PLGA-gelatin-elastin (PGE) fibers were homogeneous in appearance with an average diameter of 380 80 mn, which was considerably smaller than fibers made under identical conditions from the starting materials (PLGA, 780 +/- 200 nm; gelatin, 447 +/- 1.23 nm; elastin, 1060 170 nm). Upon hydration, PGE fibers swelled to an average fiber diameter of 963 +/- 132 nm, but did not disintegrate. Importantly, PGE scaffolds were stable in an aqueous environment without crosslinking, and were more elastic than those made of pure elastin fibers. To investigate the cytocompatibility of PGE, we cultured H9c2 rat cardiac myoblasts and rat bone marrow stromal cells (BMSCs) on fibrous PGE scaffolds. We found that myoblasts grew equally as well or slightly better on the scaffolds than on tissue-culture plastic. Microscopic evaluation confirmed that myoblasts reached confluence on the scaffold surfaces while simultaneously growing into the scaffolds.

Controlling dispersed state and exfoliation process of clay in polymer matrix

In the present review, the authors do not try to provide a comprehensive review of researches on polymer/clay nanocomposites (PCNs), but some examples to demonstrate different exfoliation processes of the clay in various polymer matrixes and the dispersed state of clay. Interaction between polymers and layered silicates plays an important role in adjusting the exfoliation process of layered silicates and the microstructure of polymer nanocomposites. Properties of polymer/layered silicate nanocomposites mainly depend on the dispersed state of layered silicates. The authors will also address the outline of the present research in the direction of PCNs including the discussion of technical problems and their possible solutions.

STATISTICAL THERMODYNAMICS IN THE FRAMEWORK OF THE LATTICE FLUID MODEL .2. BINARY MIXTURE OF POLYDISPERSE POLYMERS OF SPECIAL DISTRIBUTION

In this paper, the Gibbs free energy, the equation of state and the chemical potentials of polydisperse multicomponent polymer mixtures are derived. For general binary mixtures of polydisperse polymers, we also give the Gibbs free energy, the equation of