Monitorización de sistemas en alta disponibilidad

El objetivo del proyecto es implantar un sistema de monitorización, con la peculiaridad de encontrarse en alta disponibilidad, esto es, que el servicio (la monitorización de una infraestructura) se preste forma continua y no se vea interrumpido. Dado que el propósito del sistema es monitorizar activamente una infraestructura, ha sido necesario desplegar una infraestructura, además del sistema de monitorización. La infraestructura en cuestión está compuesta por un servidor de documentación, un servidor de base de datos, un servidor de aplicaciones y un servidor web. El sistema de monitorización se ha desplegado en la misma red de área local de esta infraestructura y monitoriza que los servicios prestados por los componentes de esta infraestructura se encuentren operativos y funcionando adecuadamente. Así pues, se tendría un sistema de monitorización local funcional. No obstante, el proyecto plantea un sistema escalable, que esté preparado para el crecimiento de la infraestructura y continúe siendo eficiente. Para ello, sistema de monitorización se encuentre dividido por dos componentes:  Sonda delegada: monitoriza localmente los activos de la infraestructura a monitorizar, es el escenario anteriormente descrito.  Sonda maestra: recibe los resultados de la monitorización realizada, este sistema puede estar desplegado en otra red distinta a la sonda delegada. Este enfoque no solo es escalable, sino también es fiel a la realidad, pues puede darse el caso de que las sondas pertenezcan a distintas infraestructuras e inclusive, distintas organizaciones, y se comuniquen a través de internet, mediante un mecanismo confiable a ser posible. El proyecto plantea que ambas sondas se encuentren en alta disponibilidad (en adelante HA, referente a high availability), y que cada sonda está compuesta por dos equipos (nodos, en adelante). Como se analizará en posteriores capítulos, existen diversas configuraciones que permiten implantar un sistema en HA, la configuración escogida para el proyecto es Activo – Pasivo(los detalles de esta configuración también se explican en posteriores capítulos). Para finalizar, se estudiara la posibilidad de ofrecer respuestas activas en ciertas situaciones y configuraciones adicionales sobre el sistema de monitorización base. Por otro lado, para la implantación del proyecto se ha usado software de código abierto para la virtualización de la infraestructura (Virtual Box y GNS3), los sistemas operativos base (Linux), el sistema de monitorización(Nagios Core) así como el software que implementa la HA (corosync y pacemaker).---ABSTRACT---The aim of the Project is to implement a monitoring system, with the peculiarity of being deployed in high availability, what it is that the service (monitoring infrastructure) is provided continuously and not interrupted. As the purpose of the system is monitoring infrastructure actively, an infrastructure has been deployed, and also the monitoring system. The infrastructure monitored is composed of a documentation server, a server database, an application server and a Web server. The monitoring system has been also deployed on the same LAN of this infrastructure and monitors the services provided by the components of this infrastructure are operational and working as expected. This is a local monitoring system functional. However, the project also proposes a scalable system that is ready for growth of infrastructure and efficient. This is the reason of divide the system in two components:  Slave Component: monitors locally the infrastructure assets to be monitored, this is the scenario described above.  Master Component: get the results from the monitoring, provided by the Slave Component. This system can be deployed in a different network than the slave component. This approach is not only scalable but also a real scenario, as may be the case that the Components belongs to different infrastructures and even, different organizations, also this components can communicate over the Internet, through a reliable mechanism if possible. The project proposes that both Components are deployed in high availability (HA onwards concerning high availability), each Component is composed of two servers (nodes, hereafter). As will be discussed in later chapters, there are several settings available to deploy a system in HA, the configuration chosen for the project is Active - Passive (details of this configuration are also explained in later chapters). Finally the possibility of offering active responses in certain situations and additional settings on the monitoring system will be discussed. On the other hand, for the implementation of the project, open source software has been used, for virtualization infrastructure (Virtual Box and GNS3), code-based operating systems (Linux), the monitoring system (Nagios core), as well as the software that implements the HA (corosync and pacemaker).

Simulación eficiente de mecanismos flexibles mediante formulaciones topológicas

El objetivo de esta Tesis es presentar un método eficiente para la evaluación de sistemas multi-cuerpo con elementos flexibles con pequeñas deformaciones, basado en métodos topológicos para la simulación de sistemas tan complejos como los que se utilizan en la práctica y en tiempo real o próximo al real. Se ha puesto un especial énfasis en la resolución eficiente de aquellos aspectos que conllevan mayor coste computacional, tales como la evaluación de las ecuaciones dinámicas y el cálculo de los términos de inercia. Las ecuaciones dinámicas se establecen en función de las variables independientes del sistema, y la integración de las mismas se realiza mediante formulaciones implícitas de index-3. Esta Tesis se articula en seis Capítulos. En el Capítulo 1 se realiza una revisión bibliográfica de la simulación de sistemas flexibles y los métodos más relevantes de integración de las ecuaciones diferenciales del movimiento. Asimismo, se presentan los objetivos de esta Tesis. En el Capítulo 2 se presenta un método semi-recursivo para la evaluación de las ecuaciones de los sistemas multi-cuerpo con elementos flexibles basado en formulaciones topológicas y síntesis modal. Esta Tesis determina la posición de cada punto del cuerpo flexible en función de un sistema de referencia flotante que se mueve con dicho cuerpo y de las amplitudes de ciertos modos de deformación calculados a partir de un mallado obtenido mediante el Método de Elementos Finitos. Se presta especial atención en las condiciones de contorno que se han de tener en cuenta a la hora de establecer las variables que definen la deformación del cuerpo flexible. El Capítulo 3 se centra en la evaluación de los términos de inercia de los sistemas flexibles que generalmente conllevan un alto coste computacional. Se presenta un método que permite el cálculo de dichos términos basado en el uso de 24 matrices constantes que pueden ser calculadas previamente al proceso de integración. Estas matrices permiten evaluar la matriz de masas y el vector de fuerzas de inercia dependientes de la velocidad sin que sea necesario evaluar la posición deformada de todos los puntos del cuerpo flexible. Se realiza un análisis pormenorizado de dichas matrices con el objetivo de optimizar su cálculo estableciendo aproximaciones que permitan reducir el número de dichos términos y optimizar aún más su evaluación. Se analizan dos posibles simplificaciones: la primera utiliza una discretización no-consistente basada en elementos finitos en los que se definen únicamente los desplazamientos axiales de los nodos; en la segunda propuesta se hace uso de una matriz de masas concentradas (Lumped Mass). Basándose en la formulación presentada, el Capítulo 4 aborda la integración eficiente de las ecuaciones dinámicas. Se presenta un método iterativo para la integración con fórmulas de index-3 basado en la proyección de las ecuaciones dinámicas según las variables independientes del sistema multi-cuerpo. El cálculo del residuo del sistema de ecuaciones no lineales que se ha de resolver de modo iterativo se realiza mediante un proceso recursivo muy eficiente que aprovecha la estructura topológica del sistema. Se analizan tres formas de evaluar la matriz tangente del citado sistema no lineal: evaluación aproximada, numérica y recursiva. El método de integración presentado permite el uso de distintas fórmulas. En esta Tesis se analizan la Regla Trapezoidal, la fórmula BDF de segundo orden y un método híbrido TR-BDF2. Para este último caso se presenta un algoritmo de paso variable. En el Capítulo 5 plantea la implementación del método propuesto en un programa general de simulación de mecanismos que permita la resolución de cualquier sistema multi-cuerpo definiéndolo mediante un fichero de datos. La implementación de este programa se ha realizado tanto en C++ como en Java. Se muestran los resultados de las formulaciones presentadas en esta Tesis mediante la simulación de cuatro ejemplos de distinta complejidad. Mediante análisis concretos se comparan la formulación presentada con otras existentes. También se analiza el efecto del lenguaje de programación utilizado en la implementación y los efectos de las posibles simplificaciones planteadas. Por último, el Capítulo 6 resume las principales conclusiones alcanzadas en la Tesis y las futuras líneas de investigación que con ella se abren. ABSTRACT This Thesis presents an efficient method for solving the forward dynamics of a multi-body sys-tem formed by rigid and flexible bodies with small strains for real-time simulation of real-life models. It is based on topological formulations. The presented work focuses on the efficient solution of the most time-consuming tasks of the simulation process, such as the numerical integration of the motion differential equations and in particular the evaluation of the inertia terms corresponding to the flexible bodies. The dynamic equations are formulated in terms of independent variables of the muti-body system, and they are integrated by means of implicit index-3 formulae. The Thesis is arranged in six chapters. Chapter 1 presents a review of the most relevant and recent contributions related to the modelization of flexible multi-body systems and the integration of the corresponding dynamic equations. The main objectives of the Thesis are also presented in detail. Chapter 2 presents a semi-recursive method for solving the equations of a multi-body system with flexible bodies based on topological formulations and modal synthesis. This Thesis uses the floating frame approach and the modal amplitudes to define the position of any point at the flexible body. These modal deformed shapes are obtained by means of the Finite Element Method. Particular attention has been taken to the boundary conditions used to define the deformation of the flexible bodies. Chapter 3 focuses on the evaluation of the inertia terms, which is usually a very time-consuming task. A new method based on the use of 24 constant matrices is presented. These matrices are evaluated during the set-up step, before the integration process. They allow the calculation of the inertia terms in terms of the position and orientation of the local coordinate system and the deformation variables, and there is no need to evaluate the position and velocities of all the nodes of the FEM mesh. A deep analysis of the inertia terms is performed in order to optimize the evaluation process, reducing both the terms used and the number of arithmetic operations. Two possible simplifications are presented: the first one uses a non-consistent approach in order to define the inertia terms respect to the Cartesian coordinates of the FEM mesh, rejecting those corresponding to the angular rotations; the second approach makes use of lumped mass matrices. Based on the previously presented formulation, Chapter 4 is focused on the numerical integration of the motion differential equations. A new predictor-corrector method based on index-3 formulae and on the use of multi-body independent variables is presented. The evaluation of the dynamic equations in a new time step needs the solution of a set on nonlinear equations by a Newton-Raphson iterative process. The computation of the corresponding residual vector is performed efficiently by taking advantage of the system’s topological structure. Three methods to compute the tangent matrix are presented: an approximated evaluation that considers only the most relevant terms, a numerical approach based on finite differences and a recursive method that uses the topological structure. The method presented for integrating the dynamic equations can use a variety of integration formulae. This Thesis analyses the use of the trapezoidal rule, the 2nd order BDF formula and the hybrid TR-BDF2 method. A variable-time step strategy is presented for the last one. Chapter 5 describes the implementation of the proposed method in a general purpose pro-gram for solving any multibody defined by a data file. This program is implemented both in C++ and Java. Four examples are used to check the validity of the formulation and to compare this method with other methods commonly used to solve the dynamic equations of multi-body systems containing flexible bodies. The efficiency of the programming methodology used and the effect of the possible simplifications proposed are also analyzed. Chapter 6 summarizes the main Conclusions obtained in this Thesis and the new lines of research that have been opened.

El Parque Agrario: estructura de preservación de los espacios agrarios en entornos urbanos en un contexto de cambio global

La propuesta del análisis de la figura de Parque Agrario en el ámbito español surge ante la constatación de que un nuevo paradigma está aconteciendo a escala estatal. Diversos focos se encuentran trabajando en paralelo, y de forma participada, en pos de la reformulación de las políticas públicas relacionadas con la agricultura periurbana. Estos focos ven en la figura de Parque Agrario un instrumento territorial que permite mejorar la sostenibilidad y cohesión territorial a través de la defensa de la gobernanza alimentaria local, sin olvidar la necesidad de conservación de los recursos naturales y el patrimonio paisajístico, junto a la prestación de múltiples servicios de los ecosistemas de estos ámbitos a la ciudadanía. Complementariamente, se empieza a vislumbrar el papel que esta figura puede desempeñar como herramienta de desarrollo territorial de los sistemas agrarios periurbanos, clave ante los efectos de carácter local que la globalización ejerce en estos territorios. La figura de Parque Agrario es una estructura que actúa bloqueando la base territorial, favoreciendo el desarrollo de la actividad agraria. Su mayor potencial es el de convertir el factor “proximidad urbana” de una amenaza a una oportunidad de desarrollo local endógeno que permita la continuidad de la agricultura, de los agricultores y del espacio agrario. La peculiaridad del Parque Agrario es que no es una figura al uso, estructurada y reglada por una legislación, sino que se trata de una iniciativa ad hoc, específica para cada caso, orientada a cumplir determinados objetivos de dinamización agraria, protección urbanística y valorización territorial. A pesar de la existencia de diversas definiciones y aportaciones sobre diferentes aspectos de la figura, no existe un análisis complejo de la misma en todas sus dimensiones, ni una tentativa de descripción de un modelo global y unitario del caso español y de sus potenciales resultados. Tampoco se han analizado en profundidad sus “invariantes” que se muestran como los elementos estructurantes del proyecto, capaces desarrollarse de forma diversa, de alcanzar diferentes niveles de complejidad, y de materializarse en función a las posibilidades que permita el marco normativo y legal. Por tanto, se plantea como objetivo principal de la tesis la definición de un modelo conceptual de Parque Agrario español, capaz de ser articulado e institucionalizado mediante un proceso de gobernanza, y que, como condición sine qua non sea duradero en el tiempo. Para poder llegar a describir un modelo colectivo se realiza, en primer lugar, un análisis genealógico que permita analizar sistemáticamente las propuestas desarrolladas en el ámbito español y los casos para establecer la existencia de una continuidad en la idea de Parque Agrario en las propuestas desarrolladas durante los últimos 25 años—sus invariantes—, y analice todos aquellos elementos que han ido enriqueciendo la figura en cada experiencia —sus variantes. Este análisis, además, ofrece como aportaciones el árbol genealógico y los mapas de dispersión de la figura y el primer catálogo de propuestas de Parque Agrario materializadas en proyecto. El resultado de la inducción de los datos obtenidos en el análisis genealógico es el modelo conceptual de Parque Agrario, que se define como una estructura orgánica de planificación-gestión-gobierno del territorio capaz de adaptarse a las necesidades específicas de todo sistema agrario periurbano que requiera la articulación-institucionalización de esta figura en él. Una vez descrito el modelo, se contrasta su fiabilidad mediante su aplicación como metodología de caracterización y evaluación de dos estudios de casos: uno exitoso, el Parque Agrario del Baix Llobregat, y uno frustrado, la propuesta de Parque Agrario de la Vega de Granada. ------------------------------------------------------ ABSTRACT -------------------------------------------------------------------- The proposed analysis of the figure of Agrarian Park in the Spanish sphere arises from the awareness that a new paradigm is happening at the state level. Different focuses are working in parallel, under participated programs, after the reformulation of public policies related to urban agriculture. These areas understand the figure of Agrarian Park as a territorial instrument for improving sustainability and territorial cohesion through the defense of local food governance, considering the need for conservation of natural resources and landscape heritage together with the multiple ecosystem services provided by these areas to the public. Additionally, the role that this figure can play as a tool for territorial development of peri-urban agrarian systems, which are key to the local effects that globalization has on these territories, is staring to be envisioned. The figure of Agrarian Park is a structure that works by blocking the territorial base to encourage the development of agrarian activity. Its greatest potential is to convert the threat of "urban proximity" into an opportunity for an endogenous local development that allows the persistence of agriculture, farmers and the agrarian space. The uniqueness of the Agrarian Park is that it is not a standard figure, structured and regulated by legislation, but rather an ad hoc initiative, specific to each case, designed to meet certain objectives of agrarian revitalization, urban protection and territorial enhance. Despite the existence of several definitions and contributions on different aspects of the figure, there is a lack of a complex analysis of it in all its dimensions, missing any attempt to describe a global and unitary model of the Spanish case and its potential outcomes. Its "invariants” have neither been evaluated in depth, shown as the structural elements of the project able to be developed in different ways, to achieve numerous levels of complexity, and to be materialized according to the possibilities allowed by the regulatory and legal framework. Therefore, the definition of a conceptual model of Spanish Agrarian Park able to be articulated and institutionalized through a process of governance, and durable over time as a sine qua non requisite, it is proposed as the main aim of the thesis. To get to describe a collective model, a genealogical analysis that systematically analyzes the proposals and cases developed in the Spanish field is undertaken to verify the existence of a continuity of the idea of Agrarian Park on the proposals developed during the past 25 years -invariants-, and evaluate all the elements that have enriched this figure in each experience - variants. This analysis also provides as inputs a family tree, maps of dispersion of the figure and the first catalog of Agrarian Park proposals materialized into projects. The result of inducting the data obtained in the genealogical analysis is the Conceptual Model of Agrarian Park, defined as an organic planning-management-government structure of the territory able to adapt to the specific needs of all peri-urban agrarian systems that require the articulation-institutionalization of this figure in it. Having described the model, its reliability is tested by applying it as a methodology for characterization and evaluation of two case studies, one successful, the Baix Llobregat Agrarian Park, and one frustrated, the proposed Agrarian Park de la Vega of Granada.

El enfoque integrado en los programas de regeneración urbana de barrios desfavorecidos: una visión relacional

Actualmente se reconoce de forma generalizada que la problemática de los barrios más desfavorecidos presenta un trasfondo estructural de magnitud multidimensional que implica a múltiples actores, que requiere respuestas de carácter multiescalar, multidimensional y multiagente y un alto nivel de cooperación institucional y de coordinación entre la diversidad de actores, que pone en cuestión los modelos de gobernanza y gestión urbana tradicionales. Frente a los retos e implicaciones que plantea la noción de espacio como construcción social y los problemas de coordinación detectados que limitan el alcance de la regeneración urbana integrada, en esta investigación se pregunta por los principales elementos que pueden facilitar la dinamización y coordinación de los procesos y se plantea que es preciso aprovechar el propio proceso participativo para reforzar el papel de estos elementos y generar mejores condiciones para la colaboración en un proceso de aprendizaje práctico de formas de análisis e intervención integrada y gestión relacional, que supere los niveles de información y consulta de la práctica participativa habitual. Se centra por tanto la atención en los factores que facilitan la dinamización y coordinación de los procesos (elementos dinamizadores y articuladores), en el nivel de participación social y cooperación institucional en los distintos momentos de la planificación de la estrategia entendida como un proceso continuo de reflexión-acción-reflexión y en aquellas condiciones del modelo de participación que pueden propiciar relaciones estratégicas y mejorar la capacidad de interacción y acción del sistema de actores y relaciones en el proceso de planificación y gestión, que lleva a cuestionar en qué medida los soportes (foros de participación y nodos y ejes de actividad y relación) y el tipo de técnicas (técnicas de dinamización y técnicas de identificación y activación de oportunidades) de los modelos de participación de experiencias concretas pueden fomentar formas de interacción que mejoren la comunicación y la movilización de recursos. Para analizar esta situación en la práctica, se desarrolla un marco conceptual, metodológico y operativo a partir de la contribución de conceptos, teorías y modelos basados en una visión relacional y multidimensional de las dinámicas de desarrollo local integrado. Esta metodología se valida en cuatro casos de referencia a partir de la información de documentos oficiales y de las visiones de la diversidad de actores implicados recogidas a través de una primera consulta a expertos (13) y entrevistas semiestructuradas a representantes de la diversidad de actores implicados (75), que se ha procesado por medio de tres herramientas conceptuales de análisis complementarias desarrolladas a lo largo de la investigación apoyadas en la perspectiva relacional de la reinterpretación actual del espacio y las escalas: el ‘Sistema de acción local’, el ‘Diagrama de momentos’ del proceso de planificación y gestión, y la ‘Matriz de caracterización’ del modelo de participación. La evaluación y la comparativa de los casos según el método de análisis evidencian la importancia del papel ejercido por los elementos articuladores (actores y organismos de enlace, espacios de encuentro y acción, y los significados y visiones compartidas) y dinamizadores (el capital social y las capacidades institucionales propiciadas en redes de colaboración) a la hora de facilitar la colaboración entre las distintas instituciones responsables, reforzar las relaciones entre actores, grupos y entidades del barrio, y mejorar los problemas de convivencia vecinal, y en definitiva, de mejorar las posibilidades de los diferentes actores urbanos de implicarse de manera activa. Además, las fórmulas alternativas que se han puesto en marcha en los distintos casos frente a las dificultades que presenta la administración a la hora de trabajar por proyectos y el resto de actores de colaborar de forma constructiva, han supuesto un importante proceso de aprendizaje y práctica en formas de participación y colaboración. ABSTRACT It is widely accepted that the current situation of multi-deprived neighborhoods has a structural and a multidimensional basis involving multiple actors that calls for multiscale, multidimensional and multiagent solutions and requires a high level of institutional cooperation and a great deal of coordination between different actors. This calls into question traditional governance and management models. In order to cope with the challenges and implications of the idea of the social construction of space and these coordination requirements that compromise the scope of integrated urban regeneration strategies, this research focuses on the main elements and factors that facilitate the mobilization and coordination of the regeneration processes, and recognizes the importance of participation processes facilitating better social, communication and collaboration skills in a practical learning process of integrated analysis and urban regeneration interventions and relational governance mechanisms going beyond mere information and consultation levels. Thus, the study focuses on the principal factors which facilitate the mobilization and coordination of integrated area regeneration, the level of social participation and institutional cooperation at different planning moments in a continuous process of reflection-action-reflection, and the conditions under which the participation process may promote strategic relationships and improve the performance of the system of actors and relationships in the planning and management process. This questions to what extent the supports (participation arenas and activity nodes and axis) and the type of techniques (participation encouragement and opportunities identification and activation techniques) considered in participation models had led to a level of interaction that has improved communication and resource mobilization capabilities. In order to analyze this situation in practice, a conceptual, methodological and operational framework is developed, which considers the contributions of the review of concepts, theories and models based on a relational and multidimensional view of local development dynamics. This methodology is validated in four representative case studies in view of official documents and the contributions of diverse stakeholders gathered through a first consultation to experts (13) and semi-structured interviews to representatives of the diversity of actors involved (75). This data has been processed by three complementary analytical tools developed during the research, based on the relational perspective of the current reinterpretation of space and scales: the 'Local Action System', the 'Diagram of planning moments', and the 'Characterization matrix of the participation model'. The comparative evaluation of the four case studies carried out using the analytical method developed during the project, shows the importance of the availability and the role of linkage elements (actors and organizations, collective spaces and shared visions) and drivers (social capital and institutional capacities generated through collaborative networks) in facilitating the collaboration among all responsible institutions, in promoting and strengthening relations between actors, groups and neighborhood organizations, and in improving the conviviality and social cohesion conditions, and ultimately in fostering the possibilities of diversity of urban actors to engage and participate actively in the transformation process, increasing accordingly the options for change. In addition, despite all the difficulties, these practices have implemented alternative formulas addressing the limitations of the administration in working by projects and not by competences and of the stakeholders in collaborating in a constructive way. The development and implementation of these participatory formulas has been an important learning process.

Formação continuada e profissionalização docente na Rede Municipal de São Paulo

Essa pesquisa situa-se no âmbito da problemática da formação continuada de professores da educação básica, especialmente daqueles que trabalham nas séries iniciais do ensino fundamental. Considerando a importância da educação continuada e a diversidade de modelos e espaços de formação de professores, que são oferecidos pela Secretaria Municipal de Educação de São Paulo, a pesquisa propõe-se a investigar experiências diversas que nesse sentido foram desenvolvidas no período pós LDBEN 9394/96, tendo por objetivo principal compreender a contribuição de tais cursos para a profissionalização e a melhoria da competência docente. A metodologia é de natureza qualitativa, envolvendo dois tipos de coleta de dados: (a) documentais: textos oficiais e outros produzidos no âmbito dos programas analisados; e (b) depoimentos orais colhidos por meio de entrevistas gravadas com um grupo de 5 professoras polivalentes do Ensino Fundamental I da rede municipal de ensino de São Paulo. A análise dos dados se respalda em vários autores que sob diferentes enfoques têm teorizado sobre o trabalho e a profissão docente, dentre os quais, Nóvoa, Dubar, Tardif, Bourdoncle e Popkewitz. A articulação entre os dados empíricos e o referencial teórico foi feita por meio dos principais conceitos que orientam a investigação: profissão, profissionalização, desenvolvimento profissional, competência e saberes docente. A expectativa é a de poder contribuir com elementos novos a propósito da questão da formação continuada de professores e, também, sobre os diferentes modelos de formação propostos para a profissionalização docente, identificando as concepções que têm se incorporado à prática dos professores por meio desse processo. As análises permitiram concluir que a educação continuada pode ser um dos elementos constitutivos do processo de profissionalização docente, no entanto, sem outras ações conjuntas torna-se inviável a sua efetivação, pois esse processo está intimamente relacionado a uma gama de fatores sociais, econômicos e políticos e a níveis elevados de rigorosidade correlacionados à formação, organização e prestígio.

"Marido espiritual" : possessão e violência simbólica no sul de Moçambique

Tese de doutoramento, Antropologia (Antropologia da Religião e do Simbólico), Universidade de Lisboa, Instituto de Ciências Sociais, 2016

Targeted delivery of heparin and LMWH using a fibrin antibody prevents restenosis

This study investigates a stent-less local delivery system for anti-restenotic agents utilizing antibodies to cross-linked fibrin (XLF). Heparin and low molecular weight heparin (LMWH) were conjugated to an antibody to cross-linked fibrin D-dinner (1D2). Rabbit right carotid arteries were injured with a balloon catheter, then the animals were given a bolus injection of 40 mug/k,g 1D2-heparin (26-70 mug/kg heparin) or 1D2-LMWH (29-80 mug/kg LMWH) conjugates or controls of saline (0.5 ml/kg), heparin (150 U/kg), LMWH (2 mg), or 1D2 (40 mug/kg), with or without a heparin bolus and sacrificed after 2 weeks (8 groups, n = 6/group). The injured artery of rabbits given 1D2-heparin or 1D2-LMWH conjugates had reduced neointimal development, with decreased luminal narrowing and positive remodelling compared with animals given control drugs. Animals given 1D2-heparin conjugate (with a heparin bolus) had three to five times more endothelial cells than the rabbits given saline or unconjugated heparin, while rabbits given 1D2-LMWH conjugate had up to 59% fewer neointimal cells than those given unconjugated drugs. There was little difference in extracellular matrix organization or composition. Thus cross-linked fibrin-antibodies can site-deliver anti-restenotic agents to injured areas of the artery wall where they influence wall remodelling and endothelial and neointimal cell number, reducing neointimal formation without systemic complications. Local delivery of anti-restenotic agents should minimise systemic effects, bleeding complications and potentially the cost of treatment due to a single, lower dose. (C) 2004 Elsevier Ireland Ltd. All rights reserved.

Green turtle somatic growth dynamics in a spatially disjunct Great Barrier Reef metapopulation

The growth dynamics of green sea turtles resident in four separate foraging grounds of the southern Great Barrier Reef genetic stock were assessed using a nonparametric regression modeling approach. Juveniles recruit to these grounds at the same size, but grow at foraging-ground-dependent rates that result in significant differences in expected size- or age-at-maturity. Mean age-at-maturity was estimated to vary from 25-50 years depending on the ground. This stock comprises mainly the same mtDNA haplotype, so geographic variability might be due to local environmental conditions rather than genetic factors, although the variability was not a function of latitudinal variation in environmental conditions or whether the food stock was seagrass or algae. Temporal variability in growth rates was evident in response to local environmental stochasticity, so geographic variability might be due to local food stock dynamics. Despite such variability, the expected size-specific growth rate function at all grounds displayed a similar nonmonotonic growth pattern with a juvenile growth spurt at 60-70 cm curved carapace length, (CCL) or 15-20 years of age. Sex-specific growth differences were also evident with females tending to grow faster than similar-sized males after the Juvenile growth spurt. It is clear that slow sex-specific growth displaying both spatial and temporal variability and a juvenile growth spurt are distinct growth behaviors of green turtles from this stock.

FLIP-SE: aspectos comunicacionais e mercadológicos do pirão cultural de Paraty

Através de pesquisas bibliográficas, entrevistas e do procedimento de observaçãoparticipante, a dissertação realiza uma análise da ocorrência de hábitos e costumes genuinamente caiçaras nas comunidades situadas no Saco do Mamanguá e na Ilha do Araújo - em Paraty - e o processo de hibridação cultural identificado nas comunidades em questão, em decorrência do turismo cultural ocasionado pela realização de eventos - mais especificamente, da Festa Literária Internacional de 'Parati' e do Circuito Paralelo de Idéias. Mostra como as comunidades aparecem representadas em tais eventos e discorre sobre a importância dada à participação em eventos tidos como culturais pela sociedade atual. Além disso, traça um diagnóstico das reais interferências ocasionadas na cultura caiçara através da atividade turística - viável aos interesses de manutenção e difusão de tal cultura - e que, por sua vez, é responsável por 80% do sistema econômico local.

Use of fundus imaging in quantification of age-related macular change

This review will discuss the use of manual grading scales, digital photography, and automated image analysis in the quantification of fundus changes caused by age-related macular disease. Digital imaging permits processing of images for enhancement, comparison, and feature quantification, and these techniques have been investigated for automated drusen analysis. The accuracy of automated analysis systems has been enhanced by the incorporation of interactive elements, such that the user is able to adjust the sensitivity of the system, or manually add and remove pixels. These methods capitalize on both computer and human image feature recognition and the advantage of computer-based methodologies for quantification. The histogram-based adaptive local thresholding system is able to extract useful information from the image without being affected by the presence of other structures. More recent developments involve compensation for fundus background reflectance, which has most recently been combined with the Otsu method of global thresholding. This method is reported to provide results comparable with manual stereo viewing. Developments in this area are likely to encourage wider use of automated techniques. This will make the grading of photographs easier and cheaper for clinicians and researchers. © 2007 Elsevier Inc. All rights reserved.

Strategies for agricultural change and the UK balance of payments

The world food crisis, Britain's reliance on imported food and feedstuffs and balance of payments difficulties were some of the factors which lent weight to the call for increased self-sufficiency in Britain's agriculture in the 1970s. This project considers two main areas: an investigation of the impact of radical agricultural change, designed to increase self-sufficiency, on the balance of payments; and, an appraisal of the potential role of the food industry within a radically different food system. The study proceeded by: an examination of the principles of agricultural policy and its development in Britain; an overview of the mechanism and meaning of the balance of payments; a consideration of the debate on agricultural import saving; the construction of radical agricultural strategies; the estimation of effects of the strategies, particularly to the balance of. payments; the role of the food industry and possible innovations within the strategies; a case study of textured vegetable proteins; and, the wider implications of implementation of radical agricultural alternatives. Two strategies were considered: a vegan system, involving no livestock; and, an intermediate system, including some livestock and dairy cattle. The study concludes that although agricultural change could in principle make a contribution to the balance of payments, implementation of agricultural change cannot be justified for this purpose alone. First, balance of payments problems can be solved by more appropriate methods. Second, the UK' s balance of payments problem has disappeared for the time being owing to North Sea oil and economic recession. Third, the political and social consequences of the changes investigated would be unacceptable. Progress in UK food policy is likely to be in the form of an integrated food and health policy.

Indwelling catheters and medical implants with FXIIIa inhibitors:a novel approach to the treatment of catheter and medical device-related infections

Central venous catheters (CVCs) are being utilized with increasing frequency in intensive care and general medical wards. In spite of the extensive experience gained in their application, CVCs are related to the long-term risks of catheter sheath formation, infection, and thrombosis (of the catheter or vessel itself) during catheterization. Such CVC-related-complications are associated with increased morbidity, mortality, duration of hospitalization, and medical care cost [1]. The present study incorporates a novel group of Factor XIIIa (FXIIIa, plasma transglutaminase) inhibitors into a lubricious silicone elastomer in order to generate an optimized drug delivery system whereby a secondary sustained drug release profile occurs following an initial burst release for catheters and other medical devices. We propose that the incorporation of FXIIIa inhibitors into catheters, stents, and other medical implant devices would reduce the incidence of catheter sheath formation, thrombotic occlusion, and associated staphylococcal infection. This technique could be used as a local delivery system for extended release with an immediate onset of action for other poorly aqueous soluble compounds. © 2012 Elsevier B.V. All rights reserved.

Loss of electricity and refrigerated foods: avoiding the danger zone

Recent events such as Winter Storm [Hurricane] Sandy and Hurricane Katrina have demonstrated that local food supplies must last as long as possible. Current recommendations are to dispose of all refrigerated food four hours after the power is lost. The purpose of this study was to determine if it is possible to safely hold food longer than four hours without power. The results indicate that the food can be held for up to six hours if the door is not opened. If ice is added to the refrigerator, then it will take the food approximately 10 hours to reach 5°C (41°F).

Relative roles of dispersal dynamics and competition in determining the isotopic niche breadth of a wetland fish

1. The niche variation hypothesis predicts that among-individual variation in niche use will increase in the presence of intraspecific competition and decrease in the presence of interspecific competition. We sought to determine whether the local isotopic niche breadth of fish inhabiting a wetland was best explained by competition for resources and the niche variation hypothesis, by dispersal of individuals from locations with different prey resources or by a combination of the two. We analysed stable isotopes of carbon and nitrogen as indices of feeding niche and compared metrics of within-site spread to characterise site-level isotopic niche breadth. We then evaluated the explanatory power of competing models of the direct and indirect effects of several environmental variables spanning gradients of disturbance, competition strength and food availability on among-individual variation of the eastern mosquitofish (Gambusia holbrooki). 2. The Dispersal model posits that only the direct effect of disturbance (i.e. changes in water level known to induce fish movement) influences among-individual variation in isotopic niche. The Partitioning model allows for only direct effects of local food availability on among-individual variation. The Combined model allows for both hypotheses by including the direct effects of disturbance and food availability. 3. A linear regression of the Combined model described more variance than models limited to the variables of either the Dispersal or Partitioning models. Of the independent variables considered, the food availability variable (per cent edible periphyton) explained the most variation in isotopic niche breadth, followed closely by the disturbance variable (days since last drying event). 4. Structural equation modelling provided further evidence that the Combined model was best supported by the data, with the Partitioning and the Dispersal models only modestly less informative. Again, the per cent edible periphyton was the variable with the largest direct effect on niche variability, with other food availability variables and the disturbance variable only slightly less important. Indirect effects of heterospecific and conspecific competitor densities were also important, through their effects on prey density. 5. Our results support the Combined hypotheses, although partitioning mechanisms appear to explain the most diet variation among individuals in the eastern mosquitofish. The results also support some predictions of the niche variation hypothesis, although both conspecific and interspecific competition appeared to increase isotopic niche breadth in contrast to predictions that interspecific competition would decrease it. We think this resulted from high diet overlap of co-occurring species, most of which consume similar macroinvertebrates.

Re-imagining the Irish foodscape

Limited attention has been paid by geographers to the Irish food system beyond the farm gate. Yet the last two decades have witnessed a substantial transformation in the provision of food and in patterns of consumption. This extended introduction to a set of four themed papers considers the role played by corporate retailing in refashioning the urban foodscape and in restructuring agri-food supply chains. The article aims to highlight the significant disconnection that exists between the realms of production and consumption, and outlines the potential of alternative visions and practices that offer a way of reconnecting them. Finally, the article will introduce the four papers which provide an illustration of the range and depth of analysis that geographers can bring to the study of the Irish food system.