Body composition studies in cystic fibrosis and myelomeningocele

One of the major considerations of improving the management of many chronic diseases has been the realisation of the importance of nutrition, and, in children, the maintenance of normal growth. Cystic Fibrosis (CF) and Myelomeningocele (MMC) are two such disease states in which nutritional status has a significant effect on morbidity.

Total body potassium in cystic fibrosis

To further evaluate the nature of malnutrition, define at-risk groups, and confirm the efficacy of nutritional supplementation on body composition in cystic fibrosis (CF), we have conducted longitudinal and cross-sectional studies of total body potassium (TBK) in 161 unselected CF subjects aged 1 month to 17 years. TBK was determined by measurement of40K in a whole body counter, reflecting body cell mass (BCM), the vital work-performing and growing cellular component of the body. Compared with normal TBK data for age and sex from pooled measurements of 1,629 healthy children aged 1 week to 17 years, CF infants (n = 12) diagnosed by newborn screening were depleted in TBK at diagnosis and showed catch-up with therapy by 1 year; CF children aged 2-17 years (n = 140) showed a tendency for inadequate accretion of TBK (and thus BCM) with increasing age, although the normal correlation between TBK and weight and height was maintained in the majority suggesting a pattern of nutritional stunting of growth; and malnourished CF children (n = 9) showed significant catchup in TBK with long-term nutritional rehabilitation (85-98% of TBK predicted for weight and height). These studies suggest that potentially serious, but possibly correctable deficits, in the growth of the body cell mass as measured by TBK occur commonly in CF. These deficits may be established very early in life and if not corrected lead to progressive nutritional growth retardation with increasing age. © 1989 Raven Press, Ltd., New York.

Short-term nutritional supplementation during management of pulmonary exacerbations in cystic fibosis: A controlled study, including effects of protein turnover

Effects of nutritional supplements on minimizing weight loss and abnormalities of protein turnover during pulmonary exacerbations in cystic fibrosis (CF) were studied by controlled trial. Patients received pulmonary therapy and either standard diet (n = 10) or adjunctive enteral supplements (n = 12). Initial protein turnover, measured by [15N]glycine kinetics, showed alterations of protein synthesis (P Syn) and catabolism (P Cat), which correlated with the degree of underweight, and negligible net protein deposition (P Dep). With treatment both groups had significant increases in mean body weight and forced expiratory volume in 1 s, expressed as percent predicted value for height (FEV1) by 3 wk, but a significant correlation between initial underweight and subsequent weight gain was observed only in supplemented patients. Mean P Syn and P Dep increased significantly (p < 0.001) only in the supplemented group. Pulmonary exacerbations in CF have important adverse effects on body-protein metabolism, similar to changes in protein-energy malnutrition and infection. These effects are reversed by short-term nutritional support. Strategic nutritional intervention should thus be considered in management, especially in malnourished patients.

The ontogeny of serum immunoreactive pancreatic lipase and cationic trypsinogen in the premature human infant

We evaluated the development of the exocrine pancreas in 16 healthy preterm infants (29.3 ± 1.6 weeks). The infants were fed breast milk with formula supplements (n=8) or formula alone (n=8). Growth was monitored weekly for 12 weeks then at 3, 6, 9, 12 months. At the same intervals sera were determined for pancreatic lipase and cationic trypsinogen. In addition, cord blood samples were analysed from another 33 preterm (27.6 ± 5.2 weeks) and 75 healthy full-term infants. Serum pancreatic lipase in the cord blood of term (3.7 ± 0.4 μg/l) and preterm infants (1.8 ± 0.2 μg/l) was significantly below values reported for older children (10.5 ± 0.9 μg/l; p < 0.001). In the preterm infant, serum lipase was also significantly lower than values obtained at term (p < 0.001). At birth, serum trypsinogen for preterm (16.8 ± 1.3 μg/l) and term infants (23.3 ± 1.9 μg/l) were below those for older children (31.4 ± 3.7 μg/l; p < 0.05). Over the first 3 weeks of life, serum lipase and trypsinogen increased significantly. From 3 weeks to 12 months of age, serum trypsinogen values remained unchanged, but serum lipase increased dramatically after 10 weeks of age. Thus, at 6 and 12 months of age, the preterm infants had significantly higher serum lipase values than infants of the same age born at term. These two pancreatic enzymes appear to show independent age-related maturation in infants born before term. The rate of maturation of lipase appears to be accelerated by exposure to the extrauterine environment.

Treatment of distal intestinal obstruction syndrome in cystic fibrosis with a balanced intestinal lavage solution

Conventional treatment of distal intestinal obstruction syndrome (DIOS) with high doses of pancreatic enzymes, mucolytic agents, and enemas is neither predictably effective nor rapid in action. In 6 cystic fibrosis patients with DIOS a balanced, non-absorbable intestinal lavage solution produced clinical and radiological improvement and striking improvement in DIOS scores. It is suggested that a balanced intestinal lavage solution should be considered as an alternative treatment for DIOS in patients with cystic fibrosis.

Fascial planes within subcutaneous fat in humans

We have used B-mode (brightness-mode) ultrasound to investigate the fascial planes within subcutaneous fat at the triceps and abdominal sites in a group of 17 women attending a weight control group over a 12 month period. In most subjects there was a single intralipid fascial plane at each site. As the thickness of adipose tissue increased, most of the change at the abdominal site was in the deep rather than the superficial layer of fat. At the triceps site both deep and superficial layers increased. Our findings confirm the presence of two different layers in human subcutaneous fat at the triceps and abdominal sites. These layers have been shown to be functionally different in animals and our study supports this in humans at the abdominal site.

The accuracy of weighing infants

Normal physiological processes can alter the weight of an infant by ± 80 g a day. Variations in measured weight and in measured changes in weight cannot be reduced by better scales or better techniques.

Reproductive Biology of Amblema neislerii, Elliptoideus sloatianus, Lampsilis subangulata, Medionidus penicillatus, and Pleurobema pyriforme (Bivalvia: Unionidae): final report

A study on the reproductive biology of Amblema neislerii, Elliptoideus sloatianus, Lampsilis subangulata, Medionidus penicillatus, and Pleurobema pyriforme was conducted from May 1995 to May 1997. The objectives of this study were as follows: 1) determine period of gravidity for each of the five mussel species, 2) determine host fish via laboratory experiments, 3) test whether unionid glochidia will transform on a nonidingenous fish, and 4) describe the glochidial morphology for each of the five mussel species using a scanning electron microscope. Amblema neislerii are tachytictic breeders and were found with mature glochidia in May. Elliptoideus sloatianus are tachytictic breeders and were found with mature glochidia from late February to early April. Lampsilis subangulata are bradytictic breeders and were found with mature glochidia from December to August. Superconglutinates were released by L. subangulata from late May to early July. Medionidus penicillatus are bradytictic breeders and were found with mature glochidia in November and February to April. Pleurobema pyriforme are tachytictic breeders and were found with mature glochidia from March to July. The following fish species served as hosts for A. neislerii: Notropis texanus, Lepomis macrochirus, L. microlophus, Micropterus salmoides, and Percina nigrofasciata. The following fish species served as hosts for E. sloatianus: Gambusia holbrooki, Poecilia reticulata, and P. nigrofasciata. The following fish species served as hosts for L. subangulata: G. holbrooki, P. reticulata, L. macrochirus, Micropterus punctulatus, and M. salmoides. The following fish species served as hosts for M. penicillatus: G. holbrooki, P. reticulata, Etheostoma edwini, and P. nigrofasciata. The following fish species served as hosts for P. pyriforme: Pteronotropis hypselopterus, G. holbrooki, and P. reticulata. Poecilia reticulata, a nonindigenous fish, served as a host for E. sloatianus, L. subangulata, M. penicillatus, and P. pyriforme. (76 page document)

Baseline aquatic faunal survey of Avon Park Air Force Range, Florida: fishes, mollusks, and crayfishes

This report presents results of the first systematic study of the diversity and distribution of fishes and mussels in Avon Park Air Force Range (APR). We also provide information on crayfishes and aquatic snails taken during our fish and mussel sampling activities. Our surveys documented the presence of 46 species of fishes (43 native and 3 nonindigenous), 9 species of mussels (including 8 native and 1 nonindigenous species), 5 species of aquatic snails, and two crayfish species. (347 page document)

O Modelo de Gestão: um estudo de caso em uma Organização Militar Prestadora de Serviço na área industrial da Marinha do Brasil

O estudo da gestão no âmbito organizacional mostra-se significativo para o entendimento das relações que se dão nas empresas e para o desenvolvimento organizacional, o que torna imprescindível sua análise no campo de estudos das práticas gerenciais, no intuito de melhor entender como práticas de gestão mais apuradas poderão melhorar o desempenho das organizações e fazê-las, assim, atingir suas metas e resultados almejados. Uma das maneiras pelas quais o Estado procura alcançar seus resultados é por meio da implementação de Políticas Públicas. No estudo da administração, é crucial que se busque o equilíbrio das dimensões políticas, organizacionais e legais desses processos. Esta pesquisa objetiva verificar o atual sistema de gestão utilizado pelo Arsenal de Marinha do Rio de Janeiro (AMRJ), uma Organização Militar Prestadora de Serviço (OMPS), que, embora seja considerada uma OMPS industrial (OMPS-I), possui uma tríplice atribuição: manutenção de meios navais, apoio de base e construção naval. Logo, são verificados os atuais óbices na sua gestão, assim como suas potencialidades, no nível estratégico, e a aplicabilidade de ferramenta gerencial que possa subsidiar a gestão estratégica da OM. O questionamento se direciona para quais aspectos, na forma de ameaças e fraquezas, influenciam na gestão do AMRJ e quais alternativas poderiam ser propostas para otimizar a sua gestão e minimizar as disfunções encontradas? Sendo assim, para que o AMRJ possa cumprir sua missão com eficiência, efetividade, economicidade e eficácia, torna-se crucial um exame do sistema atual de sua gestão, concernente à condução de suas atividades. Trata-se de uma pesquisa exploratória e descritiva, qualitativa, dedutiva, tendo sido utilizado o método de estudo de caso com pesquisa de campo e bibliográfica. Pode-se perceber que muitas são as limitações e ameaças, entretanto são significativos os pontos fortes e as oportunidades. O que norteou a pesquisa foi a busca pela maximização do resultado global da OM. Procurando ajustar o conjunto de elementos organizacionais que compõem a estrutura do AMRJ, de modo a atingir sinergia da ação isolada, porém sob a mesma orientação estratégica, de modo a permitir um alcance da efetividade, da eficácia e maior eficiência para essa importante OM pertencente à Marinha do Brasil.

Cultura e práticas de cuidado: o matriciamento em saúde mental como dispositivo de transformação do imaginário social sobre a loucura.

A pesquisa tem como tema a prática de cuidado no matriciamento em saúde mental na Estratégia da Saúde da Família. Matriciamento é uma metodologia de prática de cuidado em saúde, constituído por uma equipe de profissionais especialistas, que possui o objetivo de oferecer apoio e retaguarda à equipe de profissionais da Estratégia Saúde da Família. A hipótese é de que a prática de matriciamento em saúde mental é um dispositivo de transformação do imaginário social sobre a loucura. Trata-se de uma pesquisa de abordagem psicossocial de referencial teórico na sociologia e na antropologia para discutir os conceitos de cultura, imaginário social, práticas de cuidado e rede social, por entender que a prática de cuidado no matriciamento em saúde mental opera em rede e produz transformação no imaginário social sobre a loucura. O estudo tem, inicialmente, como base metodológica a sociologia para a análise documental da Lei 10.216/2001 Reforma Psiquiátrica brasileira - e da Portaria 154/2008 Núcleo de Apoio à Saúde da Família NASF. Em seguida, apoiada pela antropologia, a pesquisa utiliza o método etnográfico para a realização de entrevista com quatro profissionais matriciadores, tendo a análise do discurso como caminho para discutir e problematizar os dados coletados. Conclui-se, então, que o matriciamento é um dispositivo de rede e produção de cuidado que pode favorecer a transformação do imaginário social sobre a loucura. Porém, é de fundamental importância que a rede tenha funcionalidade para que isto possa ocorrer de fato, pois o matriciamento é um dispositivo de rede e de cuidado em saúde.

The Specialized Mappings Architecture

A probabilistic, nonlinear supervised learning model is proposed: the Specialized Mappings Architecture (SMA). The SMA employs a set of several forward mapping functions that are estimated automatically from training data. Each specialized function maps certain domains of the input space (e.g., image features) onto the output space (e.g., articulated body parameters). The SMA can model ambiguous, one-to-many mappings that may yield multiple valid output hypotheses. Once learned, the mapping functions generate a set of output hypotheses for a given input via a statistical inference procedure. The SMA inference procedure incorporates an inverse mapping or feedback function in evaluating the likelihood of each of the hypothesis. Possible feedback functions include computer graphics rendering routines that can generate images for given hypotheses. The SMA employs a variant of the Expectation-Maximization algorithm for simultaneous learning of the specialized domains along with the mapping functions, and approximate strategies for inference. The framework is demonstrated in a computer vision system that can estimate the articulated pose parameters of a human’s body or hands, given silhouettes from a single image. The accuracy and stability of the SMA are also tested using synthetic images of human bodies and hands, where ground truth is known.

Colony sex ratios vary with breeding structure but not relatedness asymmetry in the facultatively polygynous ant Pheidole pallidula

We investigated sex allocation in a Mediterranean population of the facultatively polygynous (multiple queen per colony) ant Pheidole pallidula. This species shows a strong split sex ratio, with most colonies producing almost exclusively a single-sex brood. Our genetic (microsatellite) analyses reveal that P. pallidula has an unusual breeding system, with colonies being headed by a single or a few unrelated queens. As expected in such a breeding system, our results show no variation in relatedness asymmetry between monogynous (single queen per colony) and polygynous colonies. Nevertheless, sex allocation was tightly associated with the breeding structure, with monogynous colonies producing a male-biased brood and polygynous colonies almost only females. In addition, sex allocation was closely correlated with colony total sexual productivity. Overall, our data show that when colonies become more productive (and presumably larger) they shift from monogyny to polygyny and from male production to female production, a pattern that has never been reported in social insects.

Investigation of population genetic structure and mating system in the ant Pheidole pallidula

The origin of eusociality in haplo-diploid organisms such as Hymenoptera has been mostly explained by kin selection. However, several studies have uncovered decreased relatedness values within colonies, resulting primarily from multiple queen matings (polyandry) and/or from the presence of more than one functional queen (polygyny). Here, we report on the use of microsatellite data for the investigation of sociogenetic parameters, such as relatedness, and levels of polygyny and polyandry, in the ant Pheidole pallidula. We demonstrate, through analysis of mother-offspring combinations and the use of direct sperm typing, that each queen is inseminated by a single male. The inbreeding coefficient within colonies and the levels of relatedness between the queens and their mate are not significantly different from zero, indicating that matings occur between unrelated individuals. Analyses of worker genotypes demonstrate that 38% of the colonies are polygynous with 2-4 functional queens, and suggest the existence of reproductive skew, i.e. unequal respective contribution of queens to reproduction. Finally, our analyses indicate that colonies are genetically differentiated and form a population exhibiting significant isolation-by-distance, suggesting that some colonies originate through budding.

Significant reproductive skew in the facultatively polygynous ant Pheidole pallidula

Reproductive skew - the extent to which reproduction is unevenly shared between individuals in a social group - varies greatly between and within animal species. In this study, we investigated how queens share parentage in polygynous (multiple queen) colonies of the Mediterranean ant Pheidole pallidula. We used highly polymorphic microsatellites markers to determine parentage of gynes (new queens), males and workers in P. pallidula field colonies. The comparison of the genotypes of young and adult workers revealed a very low queen turnover (less than 2%). The first main finding of the study of reproductive skew in these colonies was that there was a significant departure from equal contribution of queens to gyne, male and worker production. Reproductive skew was greater for male production than for queen and worker production. There was no relationship between the magnitude of the reproductive skew and the number of reproductive queens per colony, their relatedness and the overall colony productivity, some of the factors predicted to influence the extent of reproductive skew. Finally, our study revealed for the first time a trade-off in the relative contribution of nestmate queens to gyne and worker production. The queens contributing more to gyne production contributed significantly less to worker production.