A biotecnologia no setor agroindustrial brasileiro: o papel de empresas, instituições e governo no desenvolvimento da indústria de transgênicos e defensivos agrícolas

Nos últimos 40 anos, a biotecnologia tem evoluído significativamente, gerando uma série de repercussões e possibilidades para diversos setores. Embora os avanços tenham impactos muito amplos, um dos setores que mais se beneficiam do desenvolvimento da biotecnologia é a agricultura. Em meio a um contexto favorável para o desenvolvimento da biotecnologia no Brasil, buscaremos analisar o papel do setor público, de instituições de pesquisa e ensino e de empresas nacionais e transnacionais, bem como o estímulo à cooperação entre universidades e empresas do setor de defensivos agrícolas e sementes

Estratégias de preservação da memória do lugar: as políticas públicas para a industrialização, os intelectuais e a organização da cultura e os desafios da preservação do patrimônio agroindustrial em são José do Rio Preto - SP

O presente artigo tem por objetivo apresentar as relações entre as políticas públicas para a industrialização, a construção do patrimônio agroindustrial e os desafios das políticas de preservação do patrimônio em São José do Rio Preto (SP).

Mil e Uma Noites do Sertão: As Políticas Públicas para Indutrialização, a Construção do Patrimônio Agroindustrial e os Desafiios das Políticas de Preservação do Patrimônio em São José do Rio Preto - SP

Este texto tem por objetivo apresentar as relações entre as políticas públicas para a industrialização, a construção do patrimônio agroindustrial e os desafios das políticas de preservação do patrimônio em São José do Rio Preto (SP). Nesse sentido, partimos da problematização histórico-teórico do tema, as políticas públicas para a industrialização no Brasil, e seus desdobramentos, e abordamos a construção do patrimônio agroindustrial em Rio Preto, temática vinculada à preservação do patrimônio ambiental urbano , conforme Castriota (2009, p. 87). Problematizamos especificamente as questões relativas aos intelectuais e a organização da cultura , especialmente os libaneses em Rio Preto. A questão dos Intelectuais e a Organização da Cultura , sob uma perspectiva gramsciana (Gramsci, 2001), foi abordada em diversos trabalhos ao longo de nossa trajetória acadêmica (Villela, 2003; 2005; 2008). No sentido de exemplificar tais questões, escolhemos um intelectual orgânico da construção do patrimônio agroindustrial de Rio Preto, Murchid Homsi (1895-1959). Ao analisarmos as práticas destes intelectuais orgânicos , emerge a necessidade de políticas de preservação da Memória do Lugar , no sentido apresentado por Hayden (1997), especialmente do patrimônio de Rio Preto.

A região de Araraquara e a constituição do local: experiência em desenvolvimento rural a partir do SAI - Sistema Agroindustrial Integrado

The research aims to map and understand the SAI- Integrated Agroindustrial System of SEBRAE, topic program directed to small and medium "rural entrepreneurs". The SAI policy seeks targeted result saiming to qualify productive agents (smallholders) to a "mark et under construction". In the study I high light the "production location", as a constituent en vironment with own productive and defining characteristics of this location and of the productive agent. I aim to perceive the location (region of Araraquara) as a topic en vironment of a type of "alternative "agriculture, that in principle is “parallel” to the large hegemonic model of the economies of scale, of monoculture and concentrative, which in the region is taken as that of the "sugarcane industry". I understand the coexistence of these different models as central to understand the development of capitalism, which I treat as "cognitive capitalism".

Capitalismo cognitivo e a dinâmica da pequena produção agrícola alternativa no contexto local de araraquara o caso do sai sistema agroindustrial integrado do SEBRAE/SP

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Effectiveness of the compound chlorpyrifos plus cypermethrin plus citronellal against Alphitobius diaperinus. Laboratory analysis and residue determination in carcasses

Biological security and the absence of residues in meat Effectiveness, and/or eggs must be considered when recommending options for the control Alphitobius diaperinus in poultry production environments. This research study evaluated the effectiveness of cypermethrin+chlorpyrifos+citronellal in the control of A. diaperinus, including analysis for the presence of residues of this compound in poultry carcasses (experimental farm). Two studies were carried out under laboratory conditions. One used paper filters a four dilutions of the compound, and the other used a container including with pulverized broiler litter and the compound. The analysis of carcasses for residues was conducted in broilers that raised in a broiler house treated (floor and/or litter) with the compound at a dilution of 1:800. Birds were regularly sacrificed, submitted to necropsy, and liver, muscle and fat fragments were collected. Gas chromatography was used to identify the possible presence of any chemical residue in these samples. High effectiveness rates against A.diaperinus were observed in the two laboratory studies, as well as the absence of residues in the carcasses. This compound, can be recommended as a valuable used in the studied concentrations, alternative for the control and treatment of A. diaperinus.

Determination of the p-monochlorophenol residue in the calcium hydroxide plus p-monochlorophenol combination used as an intracanal dressing in pulpless teeth of dogs with induced chronic periapical lesion

To determine the presence of p-monochlorophenol in the calcium hydroxide (Calen) + p-monochlorophenol combination after its use as intracanal dressing, periapical lesions were induced in 60 root canals of upper and lower premolars of four dogs, After biomechanical preparation, the root canals received the intracanal medication, which was removed from the apical third after 2, 4, 7, and 14 days for chemical analysis by spectrophotometry, the results showed a p-monochlorophenol loss of approximately 50.0% in the dressing after 48 h, with no further significant loss after longer periods of times, p-Monochlorophenol was still present in the medication after 14 days.

Estado nutricional de mudas de baru (Dipteryx alata) em área de recuperação e remanescentes de cerrado

The Dipteryx alata is a tree specie with possibility of use in human and animal nutrition, and in the pharmaceutical industry. For reclamation of degraded areas, the revegetation has been an alternative, however, requires fertilizer addition. The objectives of this study were to evaluate the nutritional status and growth of Dipteryx alata seedlings, introduced in degraded soil under recovery process, with residues (organic and agroindustrial), compared to collected seedlings in preserved Cerrado, and evaluate the residues impact on soil chemical properties. In this work the degraded soil received the incorporation of residues, organic - RO (macrophytes) and agroindustrial - RA (ash derived from burning bagasse from sugarcane), with the following doses: 0, 16 and 32 t ha- 1 and 0, 15, 30 and 45 t ha-1 respectively. Within three months of incorporation of residues into the degraded soil, the D. alata seedlings were introduced in the experimental area, and 12 months later were evaluated for height, stem diameter, chlorophyll content and leaf contents of N, P, K, Ca, Mg, S, B, Cu, Fe, Mn and Zn. For purposes of comparison, the foliar concentration of those elements was determined in Dipteryx alata seedlings collected in preserved Cerrado area. Concomitant with the collect of leaves, at Cerrado and experimental area, soil was collected (0.0 - 0.20m deep) for evaluation of chemical parameters (P, OM, pH, K, Ca, Mg, H + Al, Al, Cu, Fe, Mn and Zn). Comparing the seedlings collected in the Cerrado with the seedlings from experimental area it is observed that the leaf concentration of N, P, K and Mg was higher in seedlings from preserved Cerrado in relation to those introduced in the experimental area. Fe, Mn and Zn, have lower foliar concentration in plants collected in the Cerrado, in the case of Mn the worst results occur in the absence of macrophytes indicating the importance of organic residue. The foliar concentration of Ca, S and Cu was similar in...

Pré-tratamento e hidrólise da casca de uva para liberação de açúcares fermentescíveis

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Aproveitamento da casca de banana na elaboração de barras de cereais: avaliação dos compostos bioativos, características físicas e sensoriais

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Optimization of in situ derivatization SPME by experimental design for GC-MS multi-residue analysis of pharmaceutical drugs in wastewater

This paper presents the development of a procedure, which enables the analysis of nine pharmaceutical drugs in wastewater using gas chromatography-mass spectrometry (GC-MS) associated with solid-phase microextraction (SPME) for the sample preparation. Experimental design was applied to optimize the in situ derivatization and the SPME extraction conditions. Ethyl chloroformate (ECF) was employed as derivatizing agent and polydimethylsiloxane-divinylbenzene (PDMS-DVB) as the SPME fiber coating. A fractional factorial design was used to evaluate the main factors for the in situ derivatization and SPME extraction. Thereafter, a Doehlert matrix design was applied to find out the best experimental conditions. The method presented a linear range from 0.5 to 10 mu g/L, and the intraday and interday precision were lower than 16%. Applicability of the method was verified from real influent and effluent samples of a wastewater treatment plant, as well as from samples of an industry wastewater and a river.

Thermostable invertases from Paecylomyces variotii produced under submerged and solid-state fermentation using agroindustrial residues

The filamentous fungus Paecylomices variotii was able to produce high levels of cell extract and extracellular invertases when grown under submerged fermentation (SbmF) and solid-state fermentation, using agroindustrial products or residues as substrates, mainly soy bran and wheat bran, at 40A degrees C for 72 h and 96 h, respectively. Addition of glucose or fructose (a parts per thousand yen1%; w/v) in SbmF inhibited enzyme production, while the addition of 1% (w/v) peptone as organic nitrogen source enhanced the production by 3.7-fold. However, 1% (w/v) (NH4)(2)HPO4 inhibited enzyme production around 80%. The extracellular form was purified until electrophoretic homogeneity (10.5-fold with 33% recovery) by DEAE-Fractogel and Sephacryl S-200 chromatography. The enzyme is a monomer with molecular mass of 102 kDa estimated by SDS-PAGE with carbohydrate content of 53.6%. Optima of temperature and pH for both, extracellular and cell extract invertases, were 60A degrees C and 4.0-4.5, respectively. Both invertases were stable for 1 h at 60A degrees C with half-lives of 10 min at 70A degrees C. Mg2+, Ba2+ and Mn2+ activated both extracellular and cell extract invertases from P. variotii. The kinetic parameters K-m and V-max for the purified extracellular enzyme corresponded to 2.5 mM and 481 U/mg prot(-1), respectively.

Effect of the inclusion of fish residue oils in diets on the fatty acid profile of muscles of males and females lambari (Astyanax altiparanae)

This study evaluated the effects of two lipids sources of fish residue (tilapia and salmon) compared with a vegetable oil source (soybean oil) on the fatty acid profiles of male and female lambari. This experiment was developed in a completely randomized experimental design in a 3 x 2 factorial arrangement, totaling 6 treatments resulting from the combination of the three experimental diets for both sexes, with four replications for each treatment. This study involved 120 male (2.58 +/- 0.13 g) and 72 female lambari (4.00 +/- 0.09 g), fed the experimental diets twice a day until apparent satiation for a period of 60 days. Oleic, linoleic, palmitic and stearic fatty acids were found at higher concentrations in all experimental oils and diets, as well in the muscle of male and female lambari. The low amounts of arachidonic, eicosapentaenoic and docosahexaenoic acids in the experimental diets and subsequent greater concentrations in muscle tissue, suggested that lambari are able to desaturate and elongate the chain of fatty acids with 18 carbons. The fish of both sexes that received the diet with soybean oil showed high levels of n-6 fatty acids, especially of C18: 2n-6 and low levels of eicosapentaenoic and docosahexaenoic acids. The diet with salmon residue oil promoted higher levels of fatty acids of the n-3 series and resulted in the best n-3/n-6 ratio in the muscle of male and female lambari. The oils from fish residues can be a substitute for traditional fish oil and its use in the lambari diets does not impair its growth.

Dasatinib Overrides Imatinib Resistance Mediated by the F359I Residue Mutation in Two Patients with Chronic Myeloid Leukemia

Despite the beneficial effects of imatinib mesylate, some patients may either not respond or respond suboptimally. Here, we report two chronic myelogenous leukemia patients; one had a suboptimal response according to European LeukemiaNet criteria (a major molecular response was not achieved after 18 months of standard-dose imatinib therapy) and the other had failure with a standard dose of imatinib. At the time of the suboptimal response in patient 1 and the failure in patient 2, we were able to detect the F359I mutation in the BCR-ABL tyrosine kinase domain using DNA sequencing in both patients. Therefore, it was decided to change the therapeutic regimen to dasatinib at a dose of 100 mg once daily in both patients. This change resulted in the achievement of complete cytogenetic remission in patient 1 after 4 months and a major molecular response within 2 and 3 months in both patients. Detection of the F359I mutation in our two cases likely explains the suboptimal response to imatinib in case 1 and the failure in case 2. This implies that in such cases dasatinib should be considered to effectively suppress the mutated clones. Copyright (C) 2011 S. Karger AG, Basel

Development of methodology for determination of pesticides residue in water by SPE/HPLC/DAD

To boost crop yield, sugarcane growers are using increasing amounts of pesticides to combat insects and weeds. But residues of these compounds can pollute water resources, such as lakes, rivers and aquifers. The present paper reports the results of a study of water samples from the Feijao River, which is the source of drinking water for the city of Sao Carlos, Sao Paulo, Brazil. The samples were evaluated for the presence of four leading pesticides - ametryn, atrazine, diuron and fipronil - used on sugarcane, the dominant culture in the region. The samples were obtained from three points along the river: the headwaters, along the middle course of the river and just before the municipal water intake station. The pesticides were extracted from the water samples by solid-phase extraction (SPE) and then analyzed by liquid chromatography with diode array detection (LC-DAD). The analytical method was validated by traditional methods, obtaining recovery values between 90 and 95%, with precision deviations inferior to 2.56%, correlation coefficients above 0.99 and detection and quantification limits varying from 0.02 to 0.05 mg L-1 and 0.07 to 0.17 mg L-1, respectively. No presence of residues of the pesticides was detected in the samples, considering the detection limits of the method employed.