Jerdonase, a novel serine protease with kinin-releasing and fibrinogenolytic activity from Trimeresurus jerdonii venom

A novel kinin-releasing and fibrin (ogen)olytic enzyme termed jerdonase was purified to homogeneity from the venom of Trimeresurus jerdonii by DEAE Sephadex A-50 anion exchange, Sephadex G-100 (superfine) gel filtration and reverse-phase high performance liquid chromatography (RP-HPLC). Jerdonase migrated as a single band with an approximate molecular weight of 55 kD under the reduced conditions and 53 kD under the non-reduced conditions. The enzyme was a glycoprotein containing 35.8% neutral carbohydrate. The N-terminal amino acid sequence of jerdonase was determined to be IIGGDECNINEHPFLVALYDA, which showed high sequence identity to other snake venom serine proteases. Jerdonase catalyzed the hydrolysis of BAEE, S-2238 and S-2302, which was inhibited by phenymethylsulfonyl fluoride (PMSF), but not affected by ethylenediaminetetraacetic acid (EDTA). Jerdonase preferentially cleaved the Aalpha-chain of human fibrinogen with lower activity towards Bbeta-chain. Moreover, the enzyme hydrolyzed bovine low-molecular-mass kininogen and releasing bradykinin. In conclusion, all results indicated that jerdonase was a multifunctional venom serine protease.

Phylogenetic relationships within mammalian order Carnivora indicated by sequences of two nuclear DNA genes

Phylogenetic relationships among 37 living species of order Carnivora spanning a relatively broad range of divergence times and taxonomic levels were examined using nuclear sequence data from exon1 of the IRBP gene (approximate to1.3 kb) and first intron

贝叶斯联合模型与中国洞穴鱼类分化时间的估算(鲤科:金线鲃属)

金线鲃属分布于中国云贵高原及周边地区,包括10多种适应于洞穴生活、具有不同程度眼睛和色素退化的典型洞穴鱼类.迄今为止,金线鲃属的系统发育关系还存在很大争议.我们获得了34种金线鲃属鱼类及外类群的细胞色素b和ND4基因序列,用贝叶斯联合模型分析数据,重建了金线鲃属的系统发育关系;并用松散分子钟估算了本属及内部分支的起源时间.金线鲃属的单系性得到强烈的支持,本属可分为6个分支.此外,本研究结果还支持洞穴物种的多系起源假说以及季氏金线鲃(Sinocyclocheilus jii)的基部位置.松散分子钟估算表明,

Simulation of polarization effects in AlGaN/GaN heterojunction

A method for introducing polarization effects in the simulation of GaN-based heterojunction devices is proposed. A delta doping layer is inserted at the interface of heterojunction and the ionized donors or acceptors act as polarization induced fixed charges. Thus polarization effects can be taken into account in a traditional device simulator. Ga-face and N-face single AlGaN/GaN heterostructures are simulated, and the simulation results show that carrier confinement takes place only in the former structure while not in the latter one. The sheet density of free electrons at the interface of Ga-face AlGaN/GaN increases with the Al composition and the thickness of AlGaN. The consistence of simulation results with the experiments and calculations reported elsewhere shows that this method can effectively introduce polarization effects in the simulation of GaN-based heterojunction devices.

Performing Fast Addition and Multiplication by Transferring Single Electrons

This paper proposes novel fast addition and multiplication circuits that are based on non-binary redundant number systems and single electron (SE) devices. The circuits consist of MOSFET-based single-electron (SE) turnstiles. We use the number of electrons to represent discrete multiple-valued logic states and we finish arithmetic operations by controlling the number of electrons transferred. We construct a compact PD2,3 adder and a 12x12bit multiplier using the PD2,3 adder. The speed of the adder can be as high as 600MHz with 400nW power dissipation. The speed of the adder is regardless of its operand length. The proposed circuits have much smaller transistors than conventional circuits.

The effects of sapphire substrates processes to the LED efficiency - art. no. 68410M

We investigate the relation between the thickness of sapphire substrates and the extraction efficiency of LED. The increasing about 5% was observed in the simulations and experiments when the sapphire thickness changed from 100um to 200um. But the output power increasing is inconspicuous when the thickness is more than 200um. The structure on bottom face of sapphire substrates can enhance the extraction efficiency of GaN-based LED, too. The difference of output power between the flip-chip LED with smooth bottom surface and the LED with roughness bottom surface is about 50%, where only a common sapphire grinding process is used. But for those LEDs grown on patterned sapphire substrate the difference is only about 10%. Another kind of periodic pattern on the bottom of sapphire is fabricated by the dry etch method, and the output of the back-etched LEDs is improved about 50% than a common. case.

一种改进的OpenMP指导调度策略研究

在科学计算中,循环结构是最重要的并行对象之一.考虑到负载平衡、调度开销等多方面因素,OpenMP标准提供静态调度、动态调度、指导调度和运行时调度等不同策略.针对指导调度策略不适合递减型循环结构的问题,提出一种改进的new_guided指导调度策略,并在OMPi编译器上加以实现.New_guided调度策略的主要思想是对前半部分的循环采用静态调度,后半部分的循环采用指导调度.针对不同循环结构,在多核处理器上对不同调度策略进行评测.结果表明,在一般情况下,OpenMP默认的静态策略的调度性能最差;对于规则的循环结构和递增的循环结构,动态调度、指导调度和new_guided策略的性能差别不大;对于递减型的循环结构,动态调度和new_guided策略的性能相当,要优于指导调度策略;对于某些极不规则的随机循环结构,动态调度明显优于其他策略,new_guided策略的性能介于动态调度和指导调度之间.

空间网络数据库中移动对象的最短路线算法

在空间网络数据库中，基于位置的服务(10cation—based services，LBS)变得越来越重要，最短路线查询是最广泛的服务请求之一．假设在网络上有一个原始对象(例如：在道路上行驶的汽车)、有一个目标对象(例如：旅店、加油站、救护车等等)，那么最短路线查询就是要找到原始对象到目标对象的最短路线．最短路线算法的研究主要分为两个方面，实时计算和预计算．在预计算方面，当前的研究对网络空间中的最短路线查询提出了多种解决方式，但是这些方法中都假设目标对象是静止的．文中在原始对象和目标对象都处于移动状态时，给出了一种最短路线查询的 算法．该算法创建了一组索引结构，将计算两个动态点之间最短路线的问题转换为计算静态边之间最短路线的问题．主要关注交通网络．但是该算法不会使用任何领域相关的信息，所以能够被应用到其他的网络环境中．从计算复杂性而言，传统的Dijkstra算法时间复杂性估计是0((i+志)2)，而该算法的相应估计是0(klbi)．

一种非线性的分布式网格资源调价算法

针对网格环境下分布式异步动态调价算法存在均衡价格收敛过程缓慢、调价效率较低的缺点,提出了一种基于市场机制的非线性调价算法。该算法结合了当前超额需求和过去超额需求对资源价格变化的影响,较真实地刻画了需求变化后资源价格的波动过程。实验证明,非线性的调价算法明显地提高了价格收敛速度,降低了调价次数。

全同1，2－聚丁二烯的合成及用~(13)C-NMR方法研究1，2－聚丁二烯的序列结构

采用Cr／Al催化体系，成功地合成了全同1，2－聚丁二烯（PBD），并用DSC方法、X－射线衍射、红外光谱及~(13)C－NMR的方法进行结构与物性测定，得如下结果：全同1，2－PBD的熔点为124.3℃；三角晶系中，分子链成了螺旋，晶胞参数为a＝17.3A，c＝ 6.5A；在红外光谱中，其特征谱带出现在694.4 cm~(-1)处；在~(13)C－NMR谱中仅出现四条谱峰，其化学位移分别为142.51、111.56、39.26、37.43 ppm。全同1，2－PBD的~(13)C－NMR谱提供的实验数据表明，在~(13)C－NMR谱中1，2－PBD－CH二碳十个五元组谱峰的归属是有别于Elgert、Kumar已有的归属。它属于一种新的归属，与半经验方法所推演的结果相符。它恰巧同聚丙烯侧甲基五元组谱峰的归属一致。采用半经验方法研究了1，2－PBD的~(13)C－NMR增中CH二碳五元组、CH_2－碳四元组及六元组共振谱峰，同时讨论了模型链的链长、温度以及立构序列的排列对各立构序列键概率的影响，求得了相应的r值。同时采用经验方法对1，2－PBD的~(13)C－NMR谱中CH_2=碳、CH－碳五元组及CH_2－碳四元组谱峰做了归属。两种方法对CH_2－碳谱峰的归属得到了一致的结果。