Chondrocyte mechanotransduction: effects of compression on deformation of intracellular organelles and relevance to cellular biosynthesis

OBJECTIVE: The effects of mechanical deformation of intact cartilage tissue on chondrocyte biosynthesis in situ have been well documented, but the mechanotransduction pathways that regulate such phenomena have not been elucidated completely. The goal of this study was to examine the effects of tissue deformation on the morphology of a range of intracellular organelles which play a major role in cell biosynthesis and metabolism. DESIGN: Using chemical fixation, high pressure freezing, and electron microscopy, we imaged chondrocytes within mechanically compressed cartilage explants at high magnification and quantitatively and qualitatively assessed changes in organelle volume and shape caused by graded levels of loading. RESULTS: Compression of the tissue caused a concomitant reduction in the volume of the extracellular matrix (ECM), chondrocyte, nucleus, rough endoplasmic reticulum, and mitochondria. Interestingly, however, the Golgi apparatus was able to resist loss of intraorganelle water and retain a portion of its volume relative to the remainder of the cell. These combined results suggest that a balance between intracellular mechanical and osmotic gradients govern the changes in shape and volume of the organelles as the tissue is compressed. CONCLUSIONS: Our results lead to the interpretive hypothesis that organelle volume changes appear to be driven mainly by osmotic interactions while shape changes are mediated by structural factors, such as cytoskeletal interactions that may be linked to extracellular matrix deformations. The observed volume and shape changes of the chondrocyte organelles and the differential behavior between organelles during tissue compression provide evidence for an important mechanotransduction pathway linking translational and post-translational events (e.g., elongation and sulfation of glycosaminoglycans (GAGs) in the Golgi) to cell deformation.

Lake sediments as natural seismographs: A compiled record of Late Quaternary earthquakes in Central Switzerland and its implication for Alpine deformation

Central Switzerland lies tectonically in an intraplate area and recurrence rates of strong earthquakes exceed the time span covered by historic chronicles. However, many lakes are present in the area that act as natural seismographs: their continuous, datable and high-resolution sediment succession allows extension of the earthquake catalogue to pre-historic times. This study reviews and compiles available data sets and results from more than 10 years of lacustrine palaeoseismological research in lakes of northern and Central Switzerland. The concept of using lacustrine mass-movement event stratigraphy to identify palaeo-earthquakes is showcased by presenting new data and results from Lake Zurich. The Late Glacial to Holocene mass-movement units in this lake document a complex history of varying tectonic and environmental impacts. Results include sedimentary evidence of three major and three minor, simultaneously triggered basin-wide lateral slope failure events interpreted as the fingerprints of palaeoseismic activity. A refined earthquake catalogue, which includes results from previous lake studies, reveals a non-uniform temporal distribution of earthquakes in northern and Central Switzerland. A higher frequency of earthquakes in the Late Glacial and Late Holocene period documents two different phases of neotectonic activity; they are interpreted to be related to isostatic post-glacial rebound and relatively recent (re-)activation of seismogenic zones, respectively. Magnitudes and epicentre reconstructions for the largest identified earthquakes provide evidence for two possible earthquake sources: (i) a source area in the region of the Alpine or Sub-Alpine Front due to release of accumulated north-west/south-east compressional stress related to an active basal thrust beneath the Aar massif; and (ii) a source area beneath the Alpine foreland due to reactivation of deep-seated strike-slip faults. Such activity has been repeatedly observed instrumentally, for example, during the most recent magnitude 4.2 and 3.5 earthquakes of February 2012, near Zug. The combined lacustrine record from northern and Central Switzerland indicates that at least one of these potential sources has been capable of producing magnitude 6.2 to 6.7 events in the past.

Ion beam analysis of as-received, H-implanted and post implanted annealed fusion steels

The elemental distribution for as-received (AR), H implanted (AI) and post-implanted annealed (A) Eurofer and ODS-Eurofer steels has been characterized by means of micro Particle Induced X-ray Emission (μ-PIXE), micro Elastic Recoil Detection (μ-ERD) and Secondary Ion Mass Spectrometry (SIMS). The temperature and time-induced H diffusion has been analyzed by Resonance Nuclear Reaction Analysis (RNRA), Thermal Desorption Spectroscopy (TDS), ERDA and SIMS techniques. μ-PIXE measurements point out the presence of inhomogeneities in the Y distribution for ODS-Eurofer samples. RNRA and SIMS experiments evidence that hydrogen easily outdiffuses in these steels even at room temperature. ERD data show that annealing at temperatures as low as 300 °C strongly accelerates the hydrogen diffusion process, driving out up to the 90% of the initial hydrogen.

Organic distributed feedback laser to monitor solvent extraction upon thermal annealing in solution-processed polymer films

Solution-processed polymer films are used in multiple technological applications. The presence of residual solvent in the film, as a consequence of the preparation method, affects the material properties, so films are typically subjected to post-deposition thermal annealing treatments aiming at its elimination. Monitoring the amount of solvent eliminated as a function of the annealing parameters is important to design a proper treatment to ensure complete solvent elimination, crucial to obtain reproducible and stable material properties and therefore, device performance. Here we demonstrate, for the first time to our knowledge, the use of an organic distributed feedback (DFB) laser to monitor with high precision the amount of solvent extracted from a spin-coated polymer film as a function of the thermal annealing time. The polymer film of interest, polystyrene in the present work, is doped with a small amount of a laser dye as to constitute the active layer of the laser device and deposited over a reusable DFB resonator. It is shown that solvent elimination translates into shifts in the DFB laser wavelength, as a consequence of changes in film thickness and refractive index. The proposed method is expected to be applicable to other types of annealing treatments, polymer-solvent combinations or film deposition methods, thus constituting a valuable tool to accurately control the quality and reproducibility of solution-processed polymer thin films.

Crash tests of sharply curved light-post guiderail. Third interim report.

Federal Highway Administration, Office of Research, Washington, D.C.

Evaluation of Potential Post-Glacial Faults Using Bare-Earth LiDAR Topographic Data Near Ridley Island, British Columbia

I present results of my evaluation to identify topographic lineaments that are potentially related to post-glacial faulting using bare-earth LiDAR topographic data near Ridley Island, British Columbia. The purpose of this evaluation has been to review bare-earth LiDAR data for evidence of post-glacial faulting in the area surrounding Ridley Island and provide a map of the potential faults to review and possibly field check. My work consisted of an extensive literature review to understand the tectonic, geologic, glacial and sea level history of the area and analysis of bare-earth LiDAR data for Ridley Island and the surrounding region. Ridley Island and the surrounding north coast of British Columbia have a long and complex tectonic and geologic history. The north coast of British Columbia consists of a series of accreted terranes and some post-accretionary deposits. The accreted terranes were attached to the North American continent during subduction of the Pacific Plate between approximately 200 Ma and 10 Ma. The terrane and post-accretionary deposits are metamorphosed sedimentary, volcanic and intrusive rocks. The rocks have experienced significant deformation and been intruded by plutonic bodies. Approximately 10 Ma subduction of the Pacific Plate beneath the North America Plate ceased along the central and north coast of British Columbia and the Queen Charlotte Fault Zone was formed. The Queen Charlotte Fault Zone is a transform-type fault that separates the Pacific Plate from the North America Plate. Within the past 1 million years, the area has experienced multiple glacial/interglacial cycles. The most recent glacial cycle occurred approximately 23,000 to 13,500 years ago. Few Quaternary deposits have been mapped in the area. The vast majority of seismicity around the northwest coast of British Columbia occurs along the Queen Charlotte Fault Zone. Numerous faults have been mapped in the area, but there is currently no evidence to suggest these faults are active (i.e. have evidence for post-glacial surface displacement or deformation). No earthquakes have been recorded within 50 km of Ridley Island. Several small earthquakes (less than magnitude 6) have been recorded within 100 km of the island. These earthquakes have not been correlated to active faults. GPS data suggests there is ongoing strain in the vicinity of Ridley Island. The strain has the potential to be released along faults, but the calculated strain may be a result of erroneous data or accommodated aseismically. Currently, the greatest known seismic hazard to Ridley Island is the Queen Charlotte Fault Zone. LiDAR data for Ridley Island, Digby Island, Lelu Island and portions of Kaien Island, Smith Island and the British Columbia mainland were reviewed and analyzed for evidence of postglacial faulting. The data showed a strong fabric across the landscape with a northwest-southeast trend that appears to mirror the observed foliation in the area. A total of 80 potential post-glacial faults were identified. Three lineaments are categorized as high, forty-one lineaments are categorized as medium and thirty-six lineaments are categorized as low. The identified features should be examined in the field to further assess potential activity. My analysis did not include areas outside of the LiDAR coverage; however faulting may be present there. LiDAR data analysis is only useful for detecting faults with surficial expressions. Faulting without obvious surficial expressions may be present in the study area.

Post-crystallization treatments for improving diffraction quality of protein crystals

X-ray crystallography is the most powerful method for determining the three-dimensional structure of biological macromolecules. One of the major obstacles in the process is the production of high-quality crystals for structure determination. All too often, crystals are produced that are of poor quality and are unsuitable for diffraction studies. This review provides a compilation of post-crystallization methods that can convert poorly diffracting crystals into data-quality crystals. Protocols for annealing, dehydration, soaking and cross-linking are outlined and examples of some spectacular changes in crystal quality are provided. The protocols are easily incorporated into the structure-determination pipeline and a practical guide is provided that shows how and when to use the different post-crystallization treatments for improving crystal quality.

Partitioning of deformation within a subduction channel during exhumation of high-pressure rocks: a case study from the Western Alps

The metamorphic belt of the Western Alps was subjected to widespread extensional tectonism at the end of the Eocene (ca. 45-35 Ma). Extension was accommodated by hinterland-directed movements along gently inclined extensional shear zones, which facilitated rapid exhumation of high-pressure and ultra-high-pressure rocks. This deformation resulted in a normal metamorphic sequence. Extension in the inner parts of the Western Alps was coeval with shortening at the front of the belt (foreland-directed thrusts), which took place during decompression, and emplaced higher grade metamorphic units over lower grade metamorphic rocks, thus forming an inverse metamorphic sequence. Two mechanisms for this extensional episode are discussed: (1) collapse of an overthickened lithosphere, and (2) internal readjustments within the orogenic wedge due to subduction channel dynamics. We favour the latter mechanism because it can account for the development of the observed inverse and normal metamorphic sequences along foreland-directed thrusts and hinterland-directed detachments, respectively. This hypothesis is supported by published structural, metamorphic and geochronological data from four geological transects through the Western Alps. This study also emphasizes the importance of post-shearing deformation (e.g. horizontal buckling versus vertical flattening), which can modify the distribution of hinterland- and foreland-directed shear zones in orogenic belts. (c) 2006 Elsevier Ltd. All rights reserved.

Deformation microstructures and recrystallization in heavily worked copper

Deformation microstructures in two batches of commercially pure copper (A and B) of allnost similar composition have been studied after rolling reductions from 5% to 95%. X- ray diffraction, optical metallography, scanning electron microscopy in the back-scattered mode, transmission and scanning electron microscopy have been used to examine the deformation microstructure. At low strains (~10 %) the deformation is accommodated by uniform octahedral slip. Microbands that occur as sheet like features usually on the {111} slip planes are formed after 10% reduction. The misorientations between rnicrobonds ond the matrix are usually small (1 - 2° ) and the dislocations within the bands suggest that a single slip system has been operative. The number of microbands increases with strain, they start to cluster and rotate after 60% reduction and, after 90 %, they become almost perfectly aligned with the rolling direction. There were no detectable differences in deformation microstructure between the two materials up to a deformation level of 60% but subsequently, copper B started to develop shear bands which became very profuse by 90% reduction. By contrast, copper A at this stage of deformation developed a smooth laminated structure. This difference in the deformation microstructures has been attributed to traces of unknown impurity in D which inhibit recovery of work hardening. The preferred orientations of both were typical of deformed copper although the presence of shear bands was associated wth a slightly weaker texture. The effects of rolling temperature and grain size on deformation microstructure were also investigated. It was concluded that lowering the rolling temperature or increasing the initial grain size encourages the material to develop shear bands after heavy deformation. Recovery and recrystallization have been studied in both materials during annealing. During recrystallization the growth of new grains showed quite different characteristics in the two cases. Where shear bands were present these acted as nucleation sites and produced a wide spread of recrystallized grain orientations. The resulting annealing textures were very weak. In the absence of shear bands, nucleation occurs by a remarkably long range bulging process which creates the cube orientation and an intensely sharp annealing texture. Cube oriented regions occur in long bands of highly elongated and well recovered cells which contain long range cumulative micorientations. They are transition bands with structural characteristics ideally suited for nucleation of recrystallization. Shear banding inhibits the cube texture both by creating alternative nuclei and by destroying the microstructural features necessary for cube nucleation.

Annealing and spectral characteristics of femtosecond laser inscribed long period gratings written into a photonic crystal fibre

A series of LPGs with the same period was inscribed by femtosecond laser into photonic crystal fibre with various powers. All suffered post-fabrication spectral evolution at low temperatures, apparently related to inscription power.

Annealing and spectral characteristics of femtosecond laser inscribed long period gratings written into a photonic crystal fibre

A series of LPGs with the same period was inscribed by femtosecond laser into photonic crystal fibre with various powers. All suffered post-fabrication spectral evolution at low temperatures, apparently related to inscription power.

Enhanced ductility and reduced asymmetry of Mg-2Al-1Zn alloy plate processed by torsion and annealing

The ductility and plastic asymmetry of an as-annealed magnesium alloy plate were studied in compression through combined process of torsion and subsequent annealing by optical microscope and EBSD. The yield strength (YS) and ultimate compression strength (UCS) as well as the compression ductility (CD) were simultaneously raised by prior torsion at room temperature. The CD was further enhanced by subsequent annealing. Also, the torqued sample followed by annealing experienced a rising CD with the increase in prior strain, leading to the maximum true strain of 0.279, which is twice that of the as-annealed original one. The sample showed a largely reduced tension-compression yield asymmetry by subjecting to pre-torsion alone or combined with a subsequent annealing. The enhanced ductility and reduced asymmetry are attributed to the development of a gradient microstructure with refined grains, and also randomization of the weakened texture due to torsion and subsequent annealing.

Equal-channel angular pressing and annealing of a twinning-induced plasticity steel : microstructure, texture, and mechanical properties

In this work, a high-manganese Fe-23Mn-1.5Al-0.3C Twinning-Induced Plasticity (TWIP) steel was subjected to plastic shear deformation using Equal-Channel Angular Pressing (ECAP) at 300 °C following route BC and additional annealing. The microstructure evolution during both deformation by ECAP and subsequent annealing was investigated and correlated with the mechanical properties. The successive grain refinement during ECAP was promoted by two parallel mechanisms, namely dislocation driven grain fragmentation and twin fragmentation, and accounted for the ultra-high strength. In addition, due to the relatively low volume fraction of deformation twins after ECAP at 300 °C, further contribution of deformation twinning during room temperature deformation allowed additional work-hardening capacity and elongation. During subsequent recovery annealing the ultra-fine grains and deformation twins were thermally stable, which supported retainment of the high yield strength along with regained uniform elongation. For the first time, the texture evolution during ECAP and during the following heat treatment was analyzed. After 1, 2, and 4 ECAP passes a transition texture with the characteristic texture components of both high- and low-SFE materials developed. During the following heat treatment the texture evolution proceeded similar to that observed in the same material after cold rolling. Retaining of the ECAP texture components due to oriented nucleation at grain boundaries and triple junctions as well as annealing twinning accounted for the formation of a weak, retained ECAP texture after recrystallization.

Hot deformation and recrystallization of a metastable beta titanium ingot

Hot deformation behavior and microstructure evolution of a coarse grain metastable beta titanium alloy (Ti-5Al-5Mo-5V-3Cr) was investigated using uniaxial compression testing followed by a subsequent beta annealing treatment. Compression testing was carried out at 720 °C and strain rates between 0.001-10 s-1 on samples with beta annealed condition and aged microstructure containing high volume fraction of relatively large alpha precipitates. The peak load of the aged samples are higher than the non-aged specimens but they show rather similar steady state flow stress. The subsequent beta annealing treatment on the compressed aged samples leads to breaking down the ingot microstructure and formation of a fully recrystallized beta phase with massive grain refinement (order of millimeter to ∼100 μm). However, after annealing such grain refinement is not seen for the non-aged samples except at high strain rates that showed partial and local recrystallization.

Self-healing thermal annealing: surface morphological restructuring control of GaN nanorods

With advances in nanolithography and dry etching, top-down methods of nanostructuring have become a widely used tool for improving the efficiency of optoelectronics. These nano dimensions can offer various benefits to the device performance in terms of light extraction and efficiency, but often at the expense of emission color quality. Broadening of the target emission peak and unwanted yellow luminescence are characteristic defect-related effects due to the ion beam etching damage, particularly for III–N based materials. In this article we focus on GaN based nanorods, showing that through thermal annealing the surface roughness and deformities of the crystal structure can be “self-healed”. Correlative electron microscopy and atomic force microscopy show the change from spherical nanorods to faceted hexagonal structures, revealing the temperature-dependent surface morphology faceting evolution. The faceted nanorods were shown to be strain- and defect-free by cathodoluminescence hyperspectral imaging, micro-Raman, and transmission electron microscopy (TEM). In-situ TEM thermal annealing experiments allowed for real time observation of dislocation movements and surface restructuring observed in ex-situ annealing TEM sampling. This thermal annealing investigation gives new insight into the redistribution path of GaN material and dislocation movement post growth, allowing for improved understanding and in turn advances in optoelectronic device processing of compound semiconductors.